Antiproliferative effects of interferon alpha on human pancreatic carcinoma cell lines are associated with differential regulation of protein kinase C isoenzymes.


BACKGROUND:The molecular mechanisms mediating the antiproliferative effects of interferon alpha on human pancreatic carcinoma cells are poorly understood. AIM:To characterise the effects of interferon alpha on protein kinase C isoenzyme expression in interferon alpha sensitive and resistant human pancreatic tumour cell lines. METHODS:The ductal human pancreatic carcinoma cell lines Capan 1 and Capan 2 were investigated. Anchorage dependent and independent growth was determined by cell number and a human tumour clonogenic assay. Interferon alpha receptor expression was examined by reverse-transcriptase polymerase chain reaction and electrophoretic mobility shift assay. Protein kinase C isoenzyme expression was evaluated by western blotting using monospecific polyclonal antibodies. RESULTS:Interferon alpha treatment results in a time and dose dependent inhibition of anchorage dependent and independent growth in Capan 1 cells while Capan 2 cells were not affected by interferon alpha. Both cell lines express interferon alpha receptor mRNA transcripts. Growth inhibition by interferon alpha in Capan 1 cells was paralleled by a profound decrease of protein kinase C alpha and zeta expression while these isoenzymes were unaffected in the interferon resistant cell line Capan 2. CONCLUSION:Inhibition of protein kinase C isoenzyme expression might determine the sensitivity of a given pancreatic carcinoma to respond to the antiproliferative action of interferon alpha.






Rosewicz S,Weder M,Kaiser A,Riecken EO




Has Abstract


1996-08-01 00:00:00














  • Zinc carnosine, a health food supplement that stabilises small bowel integrity and stimulates gut repair processes.

    abstract:BACKGROUND:Zinc carnosine (ZnC) is a health food product claimed to possess health-promoting and gastrointestinal supportive activity. Scientific evidence underlying these claims is, however, limited. AIM:To examine the effect of ZnC on various models of gut injury and repair, and in a clinical trial. METHODS:In vitr...


    pub_type: 杂志文章,随机对照试验


    authors: Mahmood A,FitzGerald AJ,Marchbank T,Ntatsaki E,Murray D,Ghosh S,Playford RJ

    更新日期:2007-02-01 00:00:00

  • Gastroparesis: separate entity or just a part of dyspepsia?

    abstract::Gastroparesis is defined by the presence of delayed gastric emptying (GE) in the absence of mechanical obstruction. Symptoms that have been attributed to gastroparesis include postprandial fullness, early satiation nausea and vomiting. Gastroprokinetic drugs are the preferred treatment option. A number of problems wit...


    pub_type: 杂志文章


    authors: Stanghellini V,Tack J

    更新日期:2014-12-01 00:00:00

  • Randomised, double blind, placebo controlled trial of intravenous antioxidant (n-acetylcysteine, selenium, vitamin C) therapy in severe acute pancreatitis.

    abstract:BACKGROUND:Based on equivocal clinical data, intravenous antioxidant therapy has been used for the treatment of severe acute pancreatitis. To date there is no randomised comparison of this therapy in severe acute pancreatitis. METHODS:We conducted a randomised, double blind, placebo controlled trial of intravenous ant...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Siriwardena AK,Mason JM,Balachandra S,Bagul A,Galloway S,Formela L,Hardman JG,Jamdar S

    更新日期:2007-10-01 00:00:00

  • Outcome of restorative proctocolectomy with ileal reservoir for ulcerative colitis: comparison of distal colitis with more proximal disease.

    abstract:BACKGROUND:An increasing number of patients with severe or refractory ulcerative colitis involving only the rectum and sigmoid colon are being offered restorative proctocolectomy with ileal reservoir but very few data are available concerning the outcome for these patients. AIM:This study was designed to compare the o...


    pub_type: 杂志文章


    authors: Samarasekera DN,Stebbing JF,Kettlewell MG,Jewell DP,Mortensen NJ

    更新日期:1996-04-01 00:00:00

  • Colonic mass movements in idiopathic chronic constipation.

    abstract::As relatively little is known of human colonic motor activity either in health, or in pathological conditions, we investigated mass movements in 14 chronically constipated patients and 18 healthy volunteers. Mass movements were recorded from proximal and distal colon during 24 h (12 noon-12 noon) by a colonoscopically...


    pub_type: 杂志文章


    authors: Bassotti G,Gaburri M,Imbimbo BP,Rossi L,Farroni F,Pelli MA,Morelli A

    更新日期:1988-09-01 00:00:00

  • Computer aided positioning of manometric probe in the upper digestive tract.

    abstract::A method not needing fluoroscopic control for positioning a manometric probe in the gastroduodenal tract is described. It is based on hydrostatic pressure variations occurring at each recording port of a multichannel perfused probe after changes in patient's position. The data are fed into a computer and the schematic...


    pub_type: 杂志文章


    authors: Bortolotti M,Bersani G

    更新日期:1988-07-01 00:00:00

  • Lymphocyte subpopulations in adult coeliac disease.

    abstract::Rosetting techniques were used to estimate T and B cell subpopulations in the peripheral blood in patients with treated and untreated adult coeliac disease and in control subjects. In patients with untreated coeliac disease, T cell numbers were significantly lower than in controls or treated patients, although there w...


    pub_type: 杂志文章


    authors: Bullen AW,Losowsky MS

    更新日期:1978-10-01 00:00:00

  • Development of chronic hepatocerebral degeneration eight years after a distal splenorenal (Warren) shunt.

    abstract::It is well known that chronic encephalopathy may be a major complication after the establishment of a surgical portal caval shunt for an episode of variceal haemorrhage. In an effort to minimise this problem Warren and colleagues developed the distal splenorenal shunt where the portal and mesenteric blood flow to the ...


    pub_type: 杂志文章


    authors: Bleasel AF,Waugh RC,McCaughan GW

    更新日期:1989-10-01 00:00:00

  • Metabolism of orally administered tauroursodeoxycholic acid in patients with primary biliary cirrhosis.

    abstract::The metabolism of tauroursodeoxycholic acid orally administered and its effects on the bile acid pool of patients with asymptomatic/mildly symptomatic primary biliary cirrhosis is described. Patients were randomly assigned 500, 1000, or 1500 mg/day of tauroursodeoxycholate for six months. Biliary and serum bile acids ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Setchell KD,Rodrigues CM,Podda M,Crosignani A

    更新日期:1996-03-01 00:00:00

  • Effect of immunisation against vasoactive intestinal polypeptide on gastric corpus tone and motility in the ferret.

    abstract::The role of vasoactive intestinal polypeptide in the control of gastric corpus tone and motility was investigated using auto-antibodies to neutralise endogenous vasoactive intestinal polypeptide. Six ferrets were immunised with vasoactive intestinal polypeptide thyroglobulin conjugate in Freund's complete adjuvant whi...


    pub_type: 杂志文章


    authors: Grundy D,Gharib-Naseri MK,Hutson D

    更新日期:1992-11-01 00:00:00

  • CDP571, a humanised monoclonal antibody to tumour necrosis factor alpha, for moderate to severe Crohn's disease: a randomised, double blind, placebo controlled trial.

    abstract:BACKGROUND:Targeting tumour necrosis factor alpha (TNF-alpha) has demonstrated efficacy in Crohn's disease. AIM:To evaluate CDP571, a humanised antibody to TNF-alpha, for treating active Crohn's disease. PATIENTS:A total of 396 patients with moderate to severe Crohn's disease. METHODS:In a 28 week, randomised, doubl...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Sandborn WJ,Feagan BG,Radford-Smith G,Kovacs A,Enns R,Innes A,Patel J

    更新日期:2004-10-01 00:00:00

  • Bone marrow transplantation improves hepatic fibrosis in Abcb4-/- mice via Th1 response and matrix metalloproteinase activity.

    abstract:OBJECTIVE:Reports on the effects of bone marrow-derived cells on hepatic fibrosis are contradictory. Impaired fibrosis but increased inflammation has recently been demonstrated 10 weeks after bone marrow transplantation (BM-Tx) in Abcb4-/- mice. It is hypothesised that BM-Tx might have long-term therapeutic potential b...


    pub_type: 杂志文章


    authors: Roderfeld M,Rath T,Pasupuleti S,Zimmermann M,Neumann C,Churin Y,Dierkes C,Voswinckel R,Barth PJ,Zahner D,Graf J,Roeb E

    更新日期:2012-06-01 00:00:00

  • Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.

    abstract:BACKGROUND:Hepatic stellate cells (HSCs) are a major fibrogenic cell type that contributes to collagen accumulation during chronic liver disease. With increasing interest in developing antifibrotic therapies, there is a need for cell lines that preserve the in vivo phenotype of human HSCs to elucidate pathways of human...


    pub_type: 杂志文章


    authors: Xu L,Hui AY,Albanis E,Arthur MJ,O'Byrne SM,Blaner WS,Mukherjee P,Friedman SL,Eng FJ

    更新日期:2005-01-01 00:00:00

  • Low dose interferon alfa-2b for chronic hepatitis B in Asian countries.

    abstract::In a study of low dose interferon alfa-2b in 587 patients expected to show a good response to treatment, 76-87% patients in different countries had alanine amino-transferase activities returned to normal after four months' treatment, 49-72% were negative for hepatitis B virus (HBV)-DNA, 51-66% were negative for hepati...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Mauracher E

    更新日期:1993-01-01 00:00:00

  • Eradication of Helicobacter pylori with clarithromycin and omeprazole.

    abstract::Clarithromycin, a new and well tolerated, acid stable macrolide antibiotic, has a similar antimicrobial spectrum to erythromycin but a better in vitro MIC90 (0.03 microgram/l-1) against Helicobacter pylori (H pylori). This study aimed at determining the eradication rate using clarithromycin 500 mg thrice daily and ome...


    pub_type: 杂志文章


    authors: Logan RP,Gummett PA,Schaufelberger HD,Greaves RR,Mendelson GM,Walker MM,Thomas PH,Baron JH,Misiewicz JJ

    更新日期:1994-03-01 00:00:00

  • Toll-like receptors 2 and 4 are differentially involved in Fas dependent apoptosis in Peyer's patch and the liver at an early stage after bile duct ligation in mice.

    abstract:BACKGROUND AND AIMS:Surgical management of extrahepatic cholestasis is frequently complicated by bacterial translocation and severe liver injury. The aim of this study was to clarify the involvement of Toll-like receptors (TLRs) in the pathogenesis of bacterial translocation and liver injury in obstructive cholestasis....


    pub_type: 杂志文章


    authors: Ogawa A,Tagawa T,Nishimura H,Yajima T,Abe T,Arai T,Taniguchi M,Takeda K,Akira S,Nimura Y,Yoshikai Y

    更新日期:2006-01-01 00:00:00

  • Blockade of the integrin alphaLbeta2 but not of integrins alpha4 and/or beta7 significantly prolongs intestinal allograft survival in mice.

    abstract:BACKGROUND:Small bowel transplantation remains a difficult therapeutic option endangered by a high rate of rejection and infectious complications. To improve these clinical results, it is mandatory to set up animal models to test alternative immunosuppressive regimens which may lead to immunotolerance. AIMS:To determi...


    pub_type: 杂志文章


    authors: Sarnacki S,Auber F,Crétolle C,Camby C,Cavazzana-Calvo M,Müller W,Wagner N,Brousse N,Révillon Y,Fischer A,Cerf-Bensussan N

    更新日期:2000-07-01 00:00:00

  • Natural history of polypoid lesions in the gall bladder.

    abstract:BACKGROUND:Although polypoid lesions of the gall bladder are frequently observed in asymptomatic subjects, the natural history of these lesions has never been studied using ultrasonography. AIM:The natural history of polypoid lesions of the gall bladder was investigated using ultrasonography. SUBJECTS:Among 4343 pati...


    pub_type: 杂志文章


    authors: Moriguchi H,Tazawa J,Hayashi Y,Takenawa H,Nakayama E,Marumo F,Sato C

    更新日期:1996-12-01 00:00:00

  • Fine needle aspiration cytology of rectal masses.

    abstract::This paper describes the results of transproctoscopic fine needle aspiration cytology in the diagnosis of rectal lesions. Fifty one consecutive patients referred with a presumptive diagnosis of rectal mass were subjected to proctoscopic examination when fine needle aspiration cytology, brush cytology and biopsy sample...


    pub_type: 杂志文章


    authors: Kochhar R,Rajwanshi A,Wig JD,Gupta NM,Kesiezie V,Bhasin DK,Malik AK,Gupta SK,Mehta SK

    更新日期:1990-03-01 00:00:00

  • miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer.

    abstract:BACKGROUND:MicroRNAs (miRNA) have potential as prognostic biomarkers and therapeutic targets in cancer. A study was undertaken to investigate the association between miRNA expression patterns and the prognosis and therapeutic outcome of colorectal cancer (CRC). METHODS:miRNA expression profiling in tumour, adenoma and...


    pub_type: 杂志文章


    authors: Ma Y,Zhang P,Wang F,Zhang H,Yang J,Peng J,Liu W,Qin H

    更新日期:2012-10-01 00:00:00

  • MicroRNA-29c mediates initiation of gastric carcinogenesis by directly targeting ITGB1.

    abstract:OBJECTIVE:Gastric cancer (GC) remains difficult to cure due to heterogeneity in a clinical challenge and the molecular mechanisms underlying this disease are complex and not completely understood. Accumulating evidence suggests that microRNAs (miRNAs) play an important role in GC, but the role of specific miRNAs involv...


    pub_type: 杂志文章


    authors: Han TS,Hur K,Xu G,Choi B,Okugawa Y,Toiyama Y,Oshima H,Oshima M,Lee HJ,Kim VN,Chang AN,Goel A,Yang HK

    更新日期:2015-02-01 00:00:00

  • Methylation of the oestrogen receptor gene in non-neoplastic epithelium as a marker of colorectal neoplasia risk in longstanding and extensive ulcerative colitis.

    abstract:BACKGROUND:Surveillance colonoscopy is widely recommended in patients with longstanding and extensive ulcerative colitis (UC) in order to detect colorectal neoplasia at an early stage. However, it still remains questionable whether surveillance colonoscopy effectively enables early detection of UC associated neoplasia....


    pub_type: 杂志文章


    authors: Fujii S,Tominaga K,Kitajima K,Takeda J,Kusaka T,Fujita M,Ichikawa K,Tomita S,Ohkura Y,Ono Y,Imura J,Chiba T,Fujimori T

    更新日期:2005-09-01 00:00:00

  • The effect of heritability and host genetics on the gut microbiota and metabolic syndrome.

    abstract:OBJECTIVE:Metabolic syndrome (MetS) arises from complex interactions between host genetic and environmental factors. Although it is now widely accepted that the gut microbiota plays a crucial role in host metabolism, current knowledge on the effect of host genetics on specific gut microbes related to MetS status remain...


    pub_type: 杂志文章


    authors: Lim MY,You HJ,Yoon HS,Kwon B,Lee JY,Lee S,Song YM,Lee K,Sung J,Ko G

    更新日期:2017-06-01 00:00:00

  • High level perinuclear antineutrophil cytoplasmic antibody (pANCA) in ulcerative colitis patients before colectomy predicts the development of chronic pouchitis after ileal pouch-anal anastomosis.

    abstract:BACKGROUND:The reported cumulative risk of developing pouchitis in ulcerative colitis (UC) patients undergoing ileal pouch-anal anastomosis (IPAA) approaches 50% after 10 years. To date, no preoperative serological predictor of pouchitis has been found. AIMS:To assess whether preoperative perinuclear antineutrophil cy...


    pub_type: 杂志文章


    authors: Fleshner PR,Vasiliauskas EA,Kam LY,Fleshner NE,Gaiennie J,Abreu-Martin MT,Targan SR

    更新日期:2001-11-01 00:00:00

  • Cholestasis and liver failure with lambda-AL amyloidosis.

    abstract::Clinically significant liver involvement in systemic amyloidosis, especially with cholestasis, is rare. We report a case of primary amyloidosis with severe intrahepatic cholestasis leading to terminal liver failure. The present case is the first of its kind reported involving Lambda light chains only as the associated...


    pub_type: 杂志文章


    authors: Konikoff F,Mor C,Stern S,Shaklai M,Halevy J,Theodor E

    更新日期:1987-07-01 00:00:00

  • Small bowel haemorrhage due to cytomegalovirus vasculitis.

    abstract::A case is described of a life threatening vasculitis of the small bowel leading to massive gastrointestinal haemorrhage which was apparently due to cytomegalovirus inclusion disease. Reactivation of cytomegalovirus probably followed the treatment of Wegener's granulomatosis with corticosteroids and azathioprine. This ...


    pub_type: 杂志文章


    authors: Sackier JM,Kelly SB,Clarke D,Rees AJ,Wood CB

    更新日期:1991-11-01 00:00:00

  • Prognosis and risk factors of metastasis in colorectal carcinoids: results of a nationwide registry over 15 years.

    abstract:BACKGROUND:Colorectal carcinoids are often described as low-grade malignant. However, no study has compared the survival between patients with colorectal carcinoids and those with carcinomas, in a large series. In addition, no global consensus has been established on the crucial determinants of metastasis in colorectal...


    pub_type: 杂志文章,多中心研究


    authors: Konishi T,Watanabe T,Kishimoto J,Kotake K,Muto T,Nagawa H,Japanese Society for Cancer of the Colon and Rectum.

    更新日期:2007-06-01 00:00:00

  • Early cancer of the gastric remnant.

    abstract::Early carcinoma of the gastric remnant was diagnosed in 19 patients between January 1976 and January 1986. In all patients early cancer was suspected at endoscopy and confirmed by biopsy and histology. The main reason for endoscopic examination was diffuse epigastric pain suggestive of stump gastritis. The surgical pr...


    pub_type: 杂志文章


    authors: Pointner R,Schwab G,Königsrainer A,Bodner E,Schmid KW

    更新日期:1988-03-01 00:00:00

  • Diagnosis of pancreatic cancer by 2[18F]-fluoro-2-deoxy-D-glucose positron emission tomography.

    abstract::The detection of pancreatic cancer or the discrimination between pancreatic cancer and chronic pancreatitis remains an important diagnostic problem. The increased glucose metabolism in malignant tumours formed the basis for this investigation, which focused on the role of positron emission tomography (PET) with 2[18F]...


    pub_type: 杂志文章


    authors: Friess H,Langhans J,Ebert M,Beger HG,Stollfuss J,Reske SN,Büchler MW

    更新日期:1995-05-01 00:00:00

  • Statistical analysis of the lactulose/breath hydrogen test in the measurement of orocaecal transit: its variability and predictive value in assessing drug action.

    abstract::The variability in the orocaecal transit time as measured by the lactulose/breath hydrogen method has been studied for three conditions: lactulose given with a meal, subjects sitting; lactulose given with a meal, subjects semirecumbent; lactulose given in aqueous solution, subjects semirecumbent. Thirty three healthy ...


    pub_type: 杂志文章


    authors: Staniforth DH,Rose D

    更新日期:1989-02-01 00:00:00