Desmoplakin expression and organization at human umbilical vein endothelial cell-to-cell junctions.

abstract：:We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

journal_title：Journal of cell science

authors： Coukell MB,Cameron AM,Adames NR

更新日期：1992-10-01 00:00:00

abstract：:Alix is a cytosolic protein in mammalian cells that was originally identified on the basis of its association with pro-apoptotic signaling. More recent evidence has established that Alix has a hand in regulating other cellular mechanisms, including endocytic membrane trafficking and cell adhesion. Although Alix appear...

journal_title：Journal of cell science

authors： Odorizzi G

更新日期：2006-08-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate [PtdIns(3)P] regulates endocytic and autophagic membrane traffic. In order to understand the downstream effects of PtdIns(3)P in these processes, it is important to identify PtdIns(3)P-binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYV...

journal_title：Journal of cell science

authors： Simonsen A,Birkeland HC,Gillooly DJ,Mizushima N,Kuma A,Yoshimori T,Slagsvold T,Brech A,Stenmark H

更新日期：2004-08-15 00:00:00

abstract：:Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Stan...

journal_title：Journal of cell science

authors： Warley A,Stephen J,Hockaday A,Appleton TC

更新日期：1983-03-01 00:00:00

abstract：:The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...

journal_title：Journal of cell science

authors： James SW,Mirabito PM,Scacheri PC,Morris NR

更新日期：1995-11-01 00:00:00

abstract：:Re-epithelialization of wounded skin is necessary for wound closure and restoration of barrier function and requires directional keratinocyte migration towards the center of the wound. The electric field (EF) generated immediately upon wounding could be the earliest signal keratinocytes receive to initiate directional...

journal_title：Journal of cell science

authors： Pullar CE,Isseroff RR

更新日期：2005-05-01 00:00:00

abstract：:Glutathione (GSH) levels progressively decline during aging and in neurodegenerative disorders. However, the contribution of such event in mediating neuronal cell death is still uncertain. In this report, we show that, in neuroblastoma cells as well as in primary mouse cortical neurons, GSH decrease, induced by buthio...

journal_title：Journal of cell science

authors： Aquilano K,Baldelli S,Cardaci S,Rotilio G,Ciriolo MR

更新日期：2011-04-01 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:In sciarid flies (Diptera, Sciaridae), one or two paternally derived X chromosomes are discarded from the soma at early cleavages to determine the sex of the embryo (XX, females; X0, males). X chromosome(s) elimination is achieved by an abnormal anaphase segregation so that X sister chromatids do not reach the poles a...

journal_title：Journal of cell science

authors： Escribá MC,Goday C

更新日期：2013-07-15 00:00:00

abstract：:RAS isoforms have been proposed to exhibit differing biological outputs due to differences in their relative occupancy of cellular organelles and signalling microdomains. The membrane binding and targeting motifs of RAS are encoded by the C-terminal hypervariable region (HVR), and the precise localisation depends upon...

journal_title：Journal of cell science

authors： Laude AJ,Prior IA

更新日期：2008-02-15 00:00:00

abstract：:Tritiated-thymidine autoradiography was used to follow the progress of cells through meiosis in male P. cinereus at 20 degrees C. Spermatocytes spend 7 days in leptotene, 5 days in zygotene and 3 days in pachytene before entering the diffuse stage. Diffuse lasts for 8 days and is followed by a diplotene of 2 days. Fir...

journal_title：Journal of cell science

authors： Morgan GT

更新日期：1979-08-01 00:00:00

abstract：:In situ observations of the development of hippocampal and cortical neurons indicate that final axon-dendrite identity is defined at the time of generation of the first two, oppositely positioned, neurites. Quite differently, in vitro studies demonstrated that axonal fate is defined by the stochastic selection of one ...

journal_title：Journal of cell science

authors： Calderon de Anda F,Gärtner A,Tsai LH,Dotti CG

更新日期：2008-01-15 00:00:00

abstract：:The essential gene noa (CG 3971; also known as Baldspot) encodes a very long chain fatty acid elongase which is most similar to the mammalian elongase ELOVL6. noa is expressed in the nervous system from embryogenesis on, in imaginal discs, the fat body, malpighian tubules and in the gonads of both sexes. Its function ...

journal_title：Journal of cell science

authors： Jung A,Hollmann M,Schäfer MA

更新日期：2007-08-15 00:00:00

abstract：:The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmo...

journal_title：Journal of cell science

authors： Niyogi S,Jimenez V,Girard-Dias W,de Souza W,Miranda K,Docampo R

更新日期：2015-06-15 00:00:00

abstract：:This study examined the role of Ca(2+) in regulatory volume decrease by Necturus erythrocytes. Hypotonic shock (50% tonicity) stimulated an increase in cytosolic free Ca(2+), detected using epi-fluorescence microscopy and the fluorescent Ca(2+) indicator fluo-4-AM (10 microM). A similar increase in fluorescence did no...

journal_title：Journal of cell science

authors： Light DB,Attwood AJ,Siegel C,Baumann NL

更新日期：2003-01-01 00:00:00

abstract：:We have examined the pattern of expression of four different matrix metalloproteinases (MMPs), collagenase, stromelysin, 92 kD gelatinase, and 72 kD gelatinase, by primary and passaged cultures of rabbit corneal fibroblasts. Primary cultures of this cell type have previously been shown to reproduce the normal tissue r...

journal_title：Journal of cell science

authors： Fini ME,Girard MT

更新日期：1990-10-01 00:00:00

abstract：:Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on the actomoysin ring and septum assembly, little information is available c...

journal_title：Journal of cell science

authors： Martín-Cuadrado AB,Dueñas E,Sipiczki M,Vázquez de Aldana CR,del Rey F

更新日期：2003-05-01 00:00:00

abstract：:The large GTPase dynamin is a mechanoenzyme that participates in the scission of nascent vesicles from the plasma membrane. Recently, dynamin has been demonstrated to associate with the Golgi apparatus in mammalian cells by morphological and biochemical methods. Additional studies using a well characterized, cell-free...

journal_title：Journal of cell science

authors： Cao H,Thompson HM,Krueger EW,McNiven MA

更新日期：2000-06-01 00:00:00

abstract：:The invasiveness of cells is correlated with the presence of dynamic actin-rich membrane structures called invadopodia, which are membrane protrusions that are associated with localized polymerization of sub-membrane actin filaments. Similar to focal adhesions and podosomes, invadopodia are cell-matrix adhesion sites....

journal_title：Journal of cell science

authors： Albiges-Rizo C,Destaing O,Fourcade B,Planus E,Block MR

更新日期：2009-09-01 00:00:00

abstract：:If mitotic-arrested, cold-stored HeLa cells are incubated at 37 degrees C a proportion of the population divides by an aberrant process which we have called subdivision by extrusion. This process has been studied by time-lapse photography and shown to differ from normal cleavage in several respects. The cell surface b...

journal_title：Journal of cell science

authors： Mullinger AM,Johnson RT

更新日期：1976-11-01 00:00:00

abstract：:Autophagy is a pivotal cytoprotective process that secures cellular homeostasis, fulfills essential roles in development, immunity and defence against pathogens, and determines the lifespan of eukaryotic organisms. However, autophagy also crucially contributes to the development of age-related human pathologies, inclu...

journal_title：Journal of cell science

authors： Proikas-Cezanne T,Takacs Z,Dönnes P,Kohlbacher O

更新日期：2015-01-15 00:00:00

abstract：:For all eukaryotic cilia the basal bodies provide a template for the assembly of the doublet microtubules, and intraflagellar transport provides a mechanism for transport of axonemal components into the growing cilium. What is not known is how the central pair of microtubules is nucleated or how their associated polyp...

journal_title：Journal of cell science

authors： Dymek EE,Smith EF

更新日期：2012-07-15 00:00:00

abstract：:In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...

journal_title：Journal of cell science

authors： Staring J,Raaben M,Brummelkamp TR

更新日期：2018-08-03 00:00:00

abstract：:Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpre...

journal_title：Journal of cell science

authors： Daboussi F,Courbet S,Benhamou S,Kannouche P,Zdzienicka MZ,Debatisse M,Lopez BS

更新日期：2008-01-15 00:00:00

abstract：:Splice variants of certain genes impact on genetic biodiversity in mammals. The tumor suppressor TP53 gene (encoding p53) plays an important role in the regulation of tumorigenesis in hepatocellular carcinoma (HCC). Δ40p53α is a naturally occurring p53 isoform that lacks the N-terminal transactivation domain, yet litt...

journal_title：Journal of cell science

authors： Ota A,Nakao H,Sawada Y,Karnan S,Wahiduzzaman M,Inoue T,Kobayashi Y,Yamamoto T,Ishii N,Ohashi T,Nakade Y,Sato K,Itoh K,Konishi H,Hosokawa Y,Yoneda M

更新日期：2017-02-01 00:00:00

abstract：:During mitosis, the cell sequentially constructs two microtubule-based spindles to ensure faithful segregation of chromosomes. A bipolar spindle first pulls apart the sister chromatids, then a central spindle further separates them away. Although the assembly of the first spindle is well described, the assembly of the...

journal_title：Journal of cell science

authors： Courthéoux T,Reboutier D,Vazeille T,Cremet JY,Benaud C,Vernos I,Prigent C

更新日期：2019-05-16 00:00:00

abstract：:We have cloned and characterized a single copy C. reinhardtii gene containing an open reading frame of 333 nucleotides encoding a 12.7 kDa protein. The novel protein, DIP13, exhibits 60% identity with two mammalian proteins, human NA14 and an unnamed mouse protein. Homologous sequences are also present in several prot...

journal_title：Journal of cell science

authors： Pfannenschmid F,Wimmer VC,Rios RM,Geimer S,Kröckel U,Leiherer A,Haller K,Nemcová Y,Mages W

更新日期：2003-04-15 00:00:00

abstract：:Cell migration and invasion play a crucial role during normal and pathological development. The expression of several members of the Ets family of transcription factors has been shown to correlate with the occurrence of these processes. In the present study, we investigated the effect of the expression of Ets1-DB, the...

journal_title：Journal of cell science

authors： Delannoy-Courdent A,Mattot V,Fafeur V,Fauquette W,Pollet I,Calmels T,Vercamer C,Boilly B,Vandenbunder B,Desbiens X

更新日期：1998-06-01 00:00:00

abstract：:The protein product of the gene responsible for ocular albinism type 1, named OA1, is a pigment-cell-specific membrane glycoprotein, displaying features of G-protein-coupled receptors, yet exclusively localized to late endosomes, lysosomes and melanosomes. To dissect the signals responsible for the intracellular local...

journal_title：Journal of cell science

authors： Piccirillo R,Palmisano I,Innamorati G,Bagnato P,Altimare D,Schiaffino MV

更新日期：2006-05-15 00:00:00

abstract：:Selective autophagy entails cooperation between target recognition and assembly of the autophagic apparatus. Target recognition is conducted by receptors that often recognize tags, such as ubiquitin and galectins, although examples of selective autophagy independent of these tags are emerging. It is less known how rec...

journal_title：Journal of cell science

authors： Kimura T,Mandell M,Deretic V

更新日期：2016-03-01 00:00:00