Desmoplakin expression and organization at human umbilical vein endothelial cell-to-cell junctions.

abstract：:Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response, viral infections, DNA damage responses and apoptosis. To date, 28 HECT E3 ubiquitin ligases have been identifi...

journal_title：Journal of cell science

authors： Wang Y,Argiles-Castillo D,Kane EI,Zhou A,Spratt DE

更新日期：2020-04-07 00:00:00

abstract：:The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two subcomplexes: a membrane-bound coat comprising sorting nexin-1 (SNX1) and possibly sort...

journal_title：Journal of cell science

authors： Bujny MV,Popoff V,Johannes L,Cullen PJ

更新日期：2007-06-15 00:00:00

abstract：:The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...

journal_title：Journal of cell science

authors： Osterwald S,Deeg KI,Chung I,Parisotto D,Wörz S,Rohr K,Erfle H,Rippe K

更新日期：2015-05-15 00:00:00

abstract：:Megasporogenesis in the heterosporous fern Marsilea (Hydropterideae) shows features intermediate between sporogenesis in homosporous ferns and that in heterosporous seed plants. The plastids in the dyads and young spores were associated with tubules 30-35 nm in diameter, probably a specialized form of endoplasmic reti...

journal_title：Journal of cell science

authors： Bell PR

更新日期：1981-10-01 00:00:00

abstract：:Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...

journal_title：Journal of cell science

authors： Gonsior SM,Platz S,Buchmeier S,Scheer U,Jockusch BM,Hinssen H

更新日期：1999-03-01 00:00:00

abstract：:The CCAAT/enhancer-binding protein beta (C/EBPbeta, also known as CEBPB) was first identified as a regulator of differentiation and inflammatory processes in adipose tissue and liver. Although C/EBPbeta was initially implicated in synaptic plasticity, its function in the brain remains largely unknown. We have previous...

journal_title：Journal of cell science

authors： Cortes-Canteli M,Luna-Medina R,Sanz-Sancristobal M,Alvarez-Barrientos A,Santos A,Perez-Castillo A

更新日期：2008-04-15 00:00:00

abstract：:The mechanical implementation of phagocytosis requires a well-coordinated deployment of cytoplasm and membrane during the creation of a phagosome. We follow the time course of this process in initially round passive neutrophils presented with antibody-coated beads of radii 1.1 to 5.5 microm. In particular, we monitor ...

journal_title：Journal of cell science

authors： Herant M,Heinrich V,Dembo M

更新日期：2005-05-01 00:00:00

abstract：:Epidermal growth factor receptor has been localized to the proliferative cell layers in a variety of stratified squamous epithelia. In the current study, the rat cornea was used as an experimental model to determine if epidermal growth factor receptor is concentrated in epithelial stem cells. Epidermal growth factor r...

journal_title：Journal of cell science

authors： Zieske JD,Wasson M

更新日期：1993-09-01 00:00:00

abstract：:Uptake of cadmium into cultured cells and its effects on cell growth and DNA synthesis are measured over a range of Cd concentrations of seven orders of magnitude. Cd uptake is found to be proportional to the external Cd concentration and to incubation time over a very broad range of concentrations. At least 200 mmol ...

journal_title：Journal of cell science

authors： von Zglinicki T,Edwall C,Ostlund E,Lind B,Nordberg M,Ringertz NR,Wroblewski J

更新日期：1992-12-01 00:00:00

abstract：:Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation toleranc...

journal_title：Journal of cell science

authors： Cardon ZG,Peredo EL,Dohnalkova AC,Gershone HL,Bezanilla M

更新日期：2018-04-10 00:00:00

abstract：:We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

journal_title：Journal of cell science

authors： Coukell MB,Cameron AM,Adames NR

更新日期：1992-10-01 00:00:00

abstract：:Eggs of Urechis caupo are surrounded by a congruent to 0.9 micrometer thick egg envelope and, attached to that, a peripheral jelly layer about 3 micrometers thick. Before fertilization, the sperm undergoes the acrosome reaction and binds to the egg envelope. As part of a study of the induction of the acrosome reaction...

journal_title：Journal of cell science

authors： Cross NL,Slezynger TC,Holland LZ

更新日期：1985-03-01 00:00:00

abstract：:The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal de...

journal_title：Journal of cell science

authors： Ferrari ME,Bernis ME,McLeod F,Podpolny M,Coullery RP,Casadei IM,Salinas PC,Rosso SB

更新日期：2018-07-09 00:00:00

abstract：:Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tig...

journal_title：Journal of cell science

authors： Murphy CR,Martin B

更新日期：1985-10-01 00:00:00

abstract：:SARA, an early endosomal protein, plays a key role in TGFβ signalling, as it presents SMAD2 and SMAD3 for phosphorylation by the activated TGFβ receptors. Here, we show that ERBIN is a new SARA-interacting protein that can be recruited by SARA to early endosomes. ERBIN was recently shown to bind and segregate phosphor...

journal_title：Journal of cell science

authors： Sflomos G,Kostaras E,Panopoulou E,Pappas N,Kyrkou A,Politou AS,Fotsis T,Murphy C

更新日期：2011-10-01 00:00:00

abstract：:When the human red cell consumes its ATP, the cell loses its discoid character in favour of a spiculated and eventually a spherical form. This discocyte-echinocyte transformation parallels both degradation of phosphatidylinositol 4,5-bisphosphate and phosphatidic acid but not dephosphorylation of cytoskeletal proteins...

journal_title：Journal of cell science

authors： Backman L

更新日期：1986-02-01 00:00:00

abstract：:In trypanosomes, the flagellum is rooted in the flagellar pocket, a surface micro-domain that is the sole site for endocytosis and exocytosis. By analysis of anterograde or retrograde intraflagellar transport in IFT88RNAi or IFT140RNAi mutant cells, we show that elongation of the new flagellum is not required for flag...

journal_title：Journal of cell science

authors： Absalon S,Blisnick T,Bonhivers M,Kohl L,Cayet N,Toutirais G,Buisson J,Robinson D,Bastin P

更新日期：2008-11-15 00:00:00

abstract：:An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...

journal_title：Journal of cell science

authors： Hearn PR,Russell RG,Farmer J

更新日期：1981-02-01 00:00:00

abstract：:Gap junctions provide direct intercellular communication by linking adjacent cells with aqueous pores permeable to molecules up to 1 kDa in molecular mass and 8-14 A in diameter. The identification of over a dozen connexins in the mammalian gap junction family has stimulated interest in the functional significance of ...

journal_title：Journal of cell science

authors： Cao F,Eckert R,Elfgang C,Nitsche JM,Snyder SA,H-ulser DF,Willecke K,Nicholson BJ

更新日期：1998-01-01 00:00:00

abstract：:Misfolded endoplasmic reticulum (ER) proteins are dislocated towards the cytosol and degraded by the ubiquitin-proteasome system in a process called ER-associated protein degradation (ERAD). During infection with human cytomegalovirus (HCMV), the viral US2 protein targets HLA class I molecules (HLA-I) for degradation ...

journal_title：Journal of cell science

authors： van de Weijer ML,Schuren ABC,van den Boomen DJH,Mulder A,Claas FHJ,Lehner PJ,Lebbink RJ,Wiertz EJHJ

更新日期：2017-09-01 00:00:00

abstract：:Tight junctions between endothelial cells of brain capillaries are the most important structural elements of the blood-brain barrier. Cultured brain endothelial cells are known to loose tight junction-dependent blood-brain barrier characteristics such as macromolecular impermeability and high electrical resistance. We...

journal_title：Journal of cell science

authors： Wolburg H,Neuhaus J,Kniesel U,Krauss B,Schmid EM,Ocalan M,Farrell C,Risau W

更新日期：1994-05-01 00:00:00

abstract：:Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension. Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood. Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to the...

journal_title：Journal of cell science

authors： Fu R,Zhu Y,Jiang X,Li Y,Zhu M,Dong M,Huang Z,Wang C,Labouesse M,Zhang H

更新日期：2018-06-11 00:00:00

abstract：:Extracellular matrix receptors of the integrin family have a dual role in the epidermis, regulating both adhesion and differentiation. Loss of contact with the extracellular matrix causes keratinocytes to become committed to terminal differentiation, and results in a decrease in the ability of the alpha 5 beta 1 integ...

journal_title：Journal of cell science

authors： Hotchin NA,Kovach NL,Watt FM

更新日期：1993-12-01 00:00:00

abstract：:Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly ...

journal_title：Journal of cell science

authors： Lone M,Kungl T,Koper A,Bottenberg W,Kammerer R,Klein M,Sweeney ST,Auburn RP,O'Kane CJ,Prokop A

更新日期：2010-07-15 00:00:00

abstract：:Actin cytoskeleton self-organization in two cell types, fibroblasts and epitheliocytes, was studied in cells confined to isotropic adhesive islands. In fibroblasts plated onto islands of optimal size, an initially circular actin pattern evolves into a radial pattern of actin bundles that undergo asymmetric chiral swir...

journal_title：Journal of cell science

authors： Jalal S,Shi S,Acharya V,Huang RY,Viasnoff V,Bershadsky AD,Tee YH

更新日期：2019-03-07 00:00:00

abstract：:We have cloned and characterized a single copy C. reinhardtii gene containing an open reading frame of 333 nucleotides encoding a 12.7 kDa protein. The novel protein, DIP13, exhibits 60% identity with two mammalian proteins, human NA14 and an unnamed mouse protein. Homologous sequences are also present in several prot...

journal_title：Journal of cell science

authors： Pfannenschmid F,Wimmer VC,Rios RM,Geimer S,Kröckel U,Leiherer A,Haller K,Nemcová Y,Mages W

更新日期：2003-04-15 00:00:00

abstract：:Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...

journal_title：Journal of cell science

authors： Malínsky J,Koberna K,Stanĕk D,Masata M,Votruba I,Raska I

更新日期：2001-02-01 00:00:00

abstract：:The Na(+)/H(+) exchanger NHE1 is involved in intracellular pH homeostasis and cell volume regulation and accumulates with actin in the lamellipodia of fibroblasts. In order to determine the role of NHE1 following epithelial transformation and the acquisition of motile and invasive properties, we studied NHE1 expressio...

journal_title：Journal of cell science

authors： Lagana A,Vadnais J,Le PU,Nguyen TN,Laprade R,Nabi IR,Noël J

更新日期：2000-10-01 00:00:00

abstract：:Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However...

journal_title：Journal of cell science

authors： Nie ZW,Niu YJ,Zhou W,Zhou DJ,Kim JY,Cui XS

更新日期：2020-12-07 00:00:00

abstract：:Dystrophic forms of epidermolysis bullosa (DEB) are associated with mutations in the type VII collagen gene (Col7a1) which encodes the major component of anchoring fibrils. To develop a DEB animal model, type VII collagen deficient mice were generated by targeted homologous recombination. The targeting vector replaced...

journal_title：Journal of cell science

authors： Heinonen S,Männikkö M,Klement JF,Whitaker-Menezes D,Murphy GF,Uitto J

更新日期：1999-11-01 00:00:00