Transformation of immortalized woodchuck hepatic cell lines with the c-Ha-ras proto-oncogene.


:Woodchuck hepatocytes were immortalized with the simian virus 40 T antigen (SV40 T-ag) oncogene and utilized in an oncogenic transformation assay. Transfection of these cell lines with an activated C-Ha-ras oncogene (EJ6.6) resulted in the transformation of cells to a phenotype characterized by anchorage-independent growth in soft agar. Colonies of transformed cells were subcloned and up to 80% were positive for oncoprotein expression detected by immunoblot and Northern blot procedures. When compared with the parental cell lines, ras-transformed derivatives were altered both morphologically and in growth rate. The tumorigenic potential of c-Ha-ras transformed cells was demonstrated in severe combined immunodeficient (SCID) mice. There was a latency period of 1 to 4 weeks before tumors were detectable and a period of 7 weeks was required for tumors to reach a diameter of 1 cm. Histologically, tumors derived from cell lines fully transformed by the SV40 T-ag had the appearance of well-differentiated hepatocellular carcinoma (HCC) while tumors derived from c-Ha-ras transformed cell lines had the appearance of poorly differentiated HCC. The capacity to induce oncogenic transformation events in immortalized woodchuck hepatic cell lines should provide the opportunity to study the cooperative effects of hepadnaviral genes in hepatocarcinogenesis in vitro.






Jacob JR,Tennant BC




Has Abstract


1996-04-01 00:00:00












  • Genetic variations in the transforming growth factor-beta pathway as predictors of survival in advanced non-small cell lung cancer.

    abstract::The magnitude of benefit is variable for advanced non-small cell lung cancer (NSCLC) patients receiving platinum-based chemotherapy. The purpose of this study is to determine whether genetic variations in the transforming growth factor-beta (TGF-β) pathway are associated with clinical outcomes in NSCLC patients receiv...


    pub_type: 杂志文章


    authors: Lin M,Stewart DJ,Spitz MR,Hildebrandt MA,Lu C,Lin J,Gu J,Huang M,Lippman SM,Wu X

    更新日期:2011-07-01 00:00:00

  • International Lung Cancer Consortium: coordinated association study of 10 potential lung cancer susceptibility variants.

    abstract:BACKGROUND:Analysis of candidate genes in individual studies has had only limited success in identifying particular gene variants that are conclusively associated with lung cancer risk. In the International Lung Cancer Consortium (ILCCO), we conducted a coordinated genotyping study of 10 common variants selected becaus...


    pub_type: 杂志文章


    authors: Truong T,Sauter W,McKay JD,Hosgood HD 3rd,Gallagher C,Amos CI,Spitz M,Muscat J,Lazarus P,Illig T,Wichmann HE,Bickeböller H,Risch A,Dienemann H,Zhang ZF,Naeim BP,Yang P,Zienolddiny S,Haugen A,Le Marchand L,Hong YC

    更新日期:2010-04-01 00:00:00

  • Two types of normal human breast epithelial cells derived from reduction mammoplasty: phenotypic characterization and response to SV40 transfection.

    abstract::A culture method to grow two morphologically distinguishable normal human breast epithelial cell types derived from reduction mammoplasty has been developed. Type I cells were characterized by a more variable cell shape, smooth cell colony boundaries, the expression of epithelial membrane antigen (EMA) and keratin 18 ...


    pub_type: 杂志文章


    authors: Kao CY,Nomata K,Oakley CS,Welsch CW,Chang CC

    更新日期:1995-03-01 00:00:00

  • Peroxiredoxin I contributes to TRAIL resistance through suppression of redox-sensitive caspase activation in human hepatoma cells.

    abstract::Reactive oxygen species (ROS) have been implicated in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) resistance of many cancers. We evaluated the role of peroxiredoxin (Prx) I in TRAIL resistance governed by coupling of nicotinamide adenosine dinucleotide phosphate oxidase (Nox)-derived ROS signaling ...


    pub_type: 杂志文章


    authors: Song IS,Kim SU,Oh NS,Kim J,Yu DY,Huang SM,Kim JM,Lee DS,Kim NS

    更新日期:2009-07-01 00:00:00

  • Identification of somatic mutations in non-small cell lung carcinomas using whole-exome sequencing.

    abstract::Lung cancer is the leading cause of cancer-related death, with non-small cell lung cancer (NSCLC) being the predominant form of the disease. Most lung cancer is caused by the accumulation of genomic alterations due to tobacco exposure. To uncover its mutational landscape, we performed whole-exome sequencing in 31 NSCL...


    pub_type: 杂志文章


    authors: Liu P,Morrison C,Wang L,Xiong D,Vedell P,Cui P,Hua X,Ding F,Lu Y,James M,Ebben JD,Xu H,Adjei AA,Head K,Andrae JW,Tschannen MR,Jacob H,Pan J,Zhang Q,Van den Bergh F,Xiao H,Lo KC,Patel J,Richmond T,Watt MA,

    更新日期:2012-07-01 00:00:00

  • Apoptosis and proliferation under conditions of deoxynucleotide pool imbalance in liver of folate/methyl deficient rats.

    abstract::Weanling male F344 rats were fed either a semi-purified diet low in methionine and lacking in choline and folic acid (folate/methyl deficient) or a supplemented control diet for periods of 2, 5, 7 days, 3 weeks, and 9 weeks. Two days after initiating the folate/methyl deficient diet in weanling F344 rats, the incidenc...


    pub_type: 杂志文章


    authors: James SJ,Miller BJ,Basnakian AG,Pogribny IP,Pogribna M,Muskhelishvili L

    更新日期:1997-02-01 00:00:00

  • Increased poly(ADP-ribose) polymerase activity during repair of (+/-)-anti-benzo[a]pyrene diolepoxide-induced DNA damage in human peripheral blood lymphocytes in vitro.

    abstract::Poly(ADP-ribose) polymerase, which catalyzes the formation of poly(ADP-ribose) polymers, is an enzyme involved in cell proliferation, differentiation and transformation as well as in recovery from DNA damage. Poly(ADP-ribose) polymers are rapidly synthesized from the ADP-ribose moieties from intracellular NAD+, which,...


    pub_type: 杂志文章


    authors: Stierum RH,van Herwijnen MH,Hageman GJ,Kleinjans JC

    更新日期:1994-04-01 00:00:00

  • Occupational exposure to heavy metals: DNA damage induction and DNA repair inhibition prove co-exposures to cadmium, cobalt and lead as more dangerous than hitherto expected.

    abstract::Co-exposure to cadmium, cobalt, lead and other heavy metals occurs in many occupational settings, such as pigment and batteries production, galvanization and recycling of electric tools. However, little is known about interactions between several heavy metals. In the present study we determined DNA single strand break...


    pub_type: 杂志文章


    authors: Hengstler JG,Bolm-Audorff U,Faldum A,Janssen K,Reifenrath M,Götte W,Jung D,Mayer-Popken O,Fuchs J,Gebhard S,Bienfait HG,Schlink K,Dietrich C,Faust D,Epe B,Oesch F

    更新日期:2003-01-01 00:00:00

  • Regulation and expression of four cytochrome P-450 isoenzymes, NADPH-cytochrome P-450 reductase, the glutathione transferases B and C and microsomal epoxide hydrolase in preneoplastic and neoplastic lesions in rat liver.

    abstract::Nitrosamine-induced hepatocarcinogenesis has been used to investigate the regulation and expression of different drug-metabolizing enzymes in preneoplastic and neoplastic lesions in the female Wistar rat. The enzymes investigated were two phenobarbital-inducible cytochrome P-450 (cyt. P-450) isoenzymes (PB1 and PB2, m...


    pub_type: 杂志文章


    authors: Buchmann A,Kuhlmann W,Schwarz M,Kunz W,Wolf CR,Moll E,Friedberg T,Oesch F

    更新日期:1985-04-01 00:00:00

  • Studies on glutathione transferases belonging to class pi in cell lines with different capacities for conjugating (+)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene.

    abstract::The glutathione transferases (GST) belonging to class pi are primarily responsible for the intracellular detoxification of the highly mutagenic and carcinogenic compound (+)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE). The aim of the present investigation was to study the n...


    pub_type: 杂志文章


    authors: Swedmark S,Romert L,Morgenstern R,Jenssen D

    更新日期:1992-10-01 00:00:00

  • Cloning, genetic mapping and expression studies of the rat Brca1 gene.

    abstract::The breast cancer gene BRCA1 has previously been cloned from both human and mouse. We cloned a fragment of the rat Brca1 homologue in order to map it and explore its biological function. Partial cDNA fragments of the rat Brca1 homologue were isolated by RT-PCR. Sequence analysis revealed that the RING-finger domain is...


    pub_type: 杂志文章


    authors: Chen KS,Shepel LA,Haag JD,Heil GM,Gould MN

    更新日期:1996-08-01 00:00:00

  • Benzoyl peroxide promotes the formation of melanotic tumors in the skin of 7,12-dimethylbenz[a]anthracene-initiated Syrian golden hamsters.

    abstract::A two-stage carcinogenesis experiment was performed in Syrian golden hamsters using a single intragastric initiation with 10 mg/kg body weight of 7,12-dimethylbenz[a]anthracene (DMBA) and repetitive topical promotion on the back skin with two different doses (80 and 160 mg, respectively) of benzoyl peroxide in 1 ml ac...


    pub_type: 杂志文章


    authors: Schweizer J,Loehrke H,Edler L,Goerttler K

    更新日期:1987-03-01 00:00:00

  • Monoclonal antibodies to 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP) and their use in the analysis of well-done fried beef.

    abstract::Four monoclonal antibodies, PhIP-1, -2, -3 and -4, have been produced that bind to PhIP (2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine), a mutagenic aromatic amine produced in meat by cooking. The immunogen was an analog of PhIP conjugated to a carrier protein through the 2-amino group of PhIP, leaving the pyridine...


    pub_type: 杂志文章


    authors: Vanderlaan M,Watkins BE,Hwang M,Knize MG,Felton JS

    更新日期:1989-12-01 00:00:00

  • Indole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene.

    abstract::The fetus and neonate are sensitive targets for chemically induced carcinogenesis. Few studies have examined the risk/benefit of chemoprotective phytochemicals, given in the maternal diet, against transplacental carcinogenesis. In this study, B6129 SF1/J (AHR(b-1/d)) and 129Sv/ImJ (AHR(d/d)) mice were cross-bred. The ...


    pub_type: 杂志文章


    authors: Yu Z,Mahadevan B,Löhr CV,Fischer KA,Louderback MA,Krueger SK,Pereira CB,Albershardt DJ,Baird WM,Bailey GS,Williams DE

    更新日期:2006-10-01 00:00:00

  • Dose-related differences in DNA adduct levels in rodent tissues following skin application of complex mixtures from air pollution sources.

    abstract::Dose-related differences in the binding of DNA reactive intermediates for three environmentally important complex mixture particulate extracts and a well-studied carcinogen, benzo[a]pyrene (BaP), were examined in female C-57 mice following multiple topical treatments ranging from 1 to 120 mg/mouse. Particulate extract...


    pub_type: 杂志文章


    authors: Gallagher JE,Jackson MA,George MH,Lewtas J

    更新日期:1990-01-01 00:00:00

  • Tumor-initiating activity, mutagenicity, and metabolism of methylated anthracenes.

    abstract::Specific methylated derivatives of anthracene are mutagenic in S. typhimurium and have tumor-initiating activity on mouse skin. In this study, the mutagenic activities of 1-, 2-, and 9-methylanthracene, 2,9- and 9,10-dimethylanthracene, 2,9,-10-trimethylanthracene, 2,3,9,10-tetramethylanthracene, and the photo-oxide o...


    pub_type: 杂志文章


    authors: La Voie EJ,Coleman DT,Rice JE,Geddie NG,Hoffmann D

    更新日期:1985-10-01 00:00:00

  • Single-nucleotide polymorphisms in selected cytokine genes and risk of adult glioma.

    abstract::A role of immunological factors in glioma etiology is suggested by reports of an inverse relationship with history of allergy or autoimmune disease. To test whether single-nucleotide polymorphisms (SNPs) in cytokine genes were related to risk of adult glioma, we genotyped 11 SNPs in seven cytokine genes within a hospi...


    pub_type: 杂志文章


    authors: Brenner AV,Butler MA,Wang SS,Ruder AM,Rothman N,Schulte PA,Chanock SJ,Fine HA,Linet MS,Inskip PD

    更新日期:2007-12-01 00:00:00

  • Polymorphisms in genes involved in GH1 release and their association with breast cancer risk.

    abstract::The regulation of growth hormone 1 (GH1) and insulin-like-growth factor-1 (IGF-1) release is under the influence of three pituitary hormones [growth hormone releasing hormone (GHRH), ghrelin (GHRL) and somatostatin (SST)], which act in an autocrine/paracrine fashion in the breast. By binding to their respective recept...


    pub_type: 杂志文章


    authors: Wagner K,Hemminki K,Grzybowska E,Klaes R,Burwinkel B,Bugert P,Schmutzler RK,Wappenschmidt B,Butkiewicz D,Pamula J,Pekala W,Försti A

    更新日期:2006-09-01 00:00:00

  • Effects produced by the non-genotoxic hepatocarcinogen methylclofenapate in dwarf mice: peroxisome induction uncoupled from DNA synthesis and nuclearity changes.

    abstract::Both Snell dwarf mice (dw/dw) and their phenotypically normal heterozygotes (dw/+) were dosed with methylclofenapate (MCP) at daily intervals by gavage (25 mg/kg). Animals were killed at 12, 24, 36 and 72 h after the initial dose and the parameters of ploidy, nuclearity and DNA synthesis were measured in hepatocytes i...


    pub_type: 杂志文章


    authors: Styles JA,Kelly MD,Pritchard NR,Foster JR

    更新日期:1990-03-01 00:00:00

  • A COX-2 inhibitor prevents the esophageal inflammation-metaplasia-adenocarcinoma sequence in rats.

    abstract::Barrett's esophagus (BE) and esophageal adenocarcinoma (ADC) is associated with reflux of duodenal contents. Cyclooxygenase (COX)-2 is over-expressed in BE and ADC, and supposedly contributes to esophageal carcinogenesis. The aim of this study is to investigate what effect a COX-2 inhibitor has on esophageal adenocarc...


    pub_type: 杂志文章


    authors: Oyama K,Fujimura T,Ninomiya I,Miyashita T,Kinami S,Fushida S,Ohta T,Koichi M

    更新日期:2005-03-01 00:00:00

  • Absence of mutations in the functional parts of the p120-GAP gene in carcinogen-induced mouse liver tumors.

    abstract::The GTPase activating proteins (GAP) stimulate the intrinsic GTPase activity of ras-p21 thus converting the protein into its inactive form. We have now analyzed carcinogen-induced mouse liver tumors for the possible occurrence of mutational changes in one of the two known GAP genes, namely p120-GAP. RNA from a total o...


    pub_type: 杂志文章


    authors: Müller O,Kress S,Schwarz M

    更新日期:1992-10-01 00:00:00

  • Enhancement of dimethylnitrosamine-initiated hepatocarcinogenesis in hamsters by subsequent administration of carbon tetrachloride but not phenobarbital or p,p'-dichlorodiphenyltrichloroethane.

    abstract::The effect of phenobarbital (PB), p,p'-dichlorodiphenyltrichloroethane (DDT) or carbon tetrachloride (CCl4) on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in Syrian golden hamsters was examined. Hamsters were given a single injection of DMN (6 mg/kg body wt) followed by either PB or DDT in the diet or repea...


    pub_type: 杂志文章


    authors: Tanaka T,Mori H,Williams GM

    更新日期:1987-09-01 00:00:00

  • Association between expression of transforming growth factor-alpha and progression of hepatocellular foci to neoplasms.

    abstract::Hepatocarcinogenesis was initiated in rats with a single dose of either of two chemical mutagens--benzo[a]pyrene diolepoxide I and methyl(acetoxymethyl)nitrosamine--administered 15 h after partial hepatectomy. The development of hepatocellular foci and neoplasms was then promoted with dietary phenobarbital given for 4...


    pub_type: 杂志文章


    authors: Kaufmann WK,Zhang Y,Kaufman DG

    更新日期:1992-08-01 00:00:00

  • Organ-specific oxidative DNA damage associated with normal birth in rats.

    abstract::Mammalian DNA contains bulky endogenous DNA modifications (I-compounds), which increase with age in unexposed animals, as shown by 32P-postlabeling. We have examined the perinatal formation of a subclass (type II) of I-compounds in rat liver, kidney, skin and lung. These I-compounds represent bulky oxidative DNA lesio...


    pub_type: 杂志文章


    authors: Randerath E,Zhou GD,Randerath K

    更新日期:1996-12-01 00:00:00

  • 5-Aminolevulinic acid mediates the in vivo and in vitro formation of 8-hydroxy-2'-deoxyguanosine in DNA.

    abstract::5-Aminolevulinic acid (ALA), a heme precursor accumulated in chemical and inborn porphyrias, may behave as an endogenous pro-oxidant. In chronically treated rats (40 mg ALA/kg body wt every 2 days for 15 days) the steady-state level of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in liver DNA (94.5 +/- 23.3 residues/10(6) dG)...


    pub_type: 杂志文章


    authors: Fraga CG,Onuki J,Lucesoli F,Bechara EJ,Di Mascio P

    更新日期:1994-10-01 00:00:00

  • The contribution of alternative polyadenylation to the cancer phenotype.

    abstract::Eukaryotic 3'-end formation is a critical step for mRNA maturation and involves a requisite cleavage and polyadenylation event downstream of a polyadenylation signal. Recent discoveries suggest that in nearly 70% of human genes, cleavage and polyadenylation can occur at multiple locations through a process known as al...


    pub_type: 杂志文章,评审


    authors: Masamha CP,Wagner EJ

    更新日期:2018-01-12 00:00:00

  • Interaction between polycyclic aromatic hydrocarbons, crude oil and oil dispersants in the Salmonella mutagenesis assay.

    abstract::Mutagenicity assays were carried out in the Salmonella/microsome test, using five S. typhimurium his(-) strains (TA1535, TA1537, TA1538, TA98 and TA100), both in the presence and absence of post-mitochondrial preparations from Aroclor-induced rat livers and suitable co-factors. Seven oil dispersants showed a wide rang...


    pub_type: 杂志文章


    authors: Petrilli FL,De Renzi GP,De Flora S

    更新日期:1980-01-01 00:00:00

  • Cyclosporine promotes the induction of thymic lymphomas in C57BL/6 mice initiated by a single dose of gamma-radiation.

    abstract::We previously demonstrated that a single dose of gamma-radiation (350 rads) was able to induce thymic lymphomas in C57BL mice when followed by promoting treatment with oral cyclosporine (CsA), a non-genotoxic immunosuppressant. We have now tested the efficacy of various doses of gamma-radiation as an initiator of CsA ...


    pub_type: 杂志文章


    authors: Yabu K,Warty VS,Gorelik E,Shinozuka H

    更新日期:1991-01-01 00:00:00

  • Tobacco and estrogen metabolic polymorphisms and risk of non-small cell lung cancer in women.

    abstract::To explore the potential role for estrogen in lung cancer susceptibility, candidate single-nucleotide polymorphism (SNPs) in tobacco and estrogen metabolism genes were evaluated. Population-based cases (n = 504) included women aged 18-74, diagnosed with NSCLC in metropolitan Detroit between November 2001 and October 2...


    pub_type: 杂志文章


    authors: Cote ML,Yoo W,Wenzlaff AS,Prysak GM,Santer SK,Claeys GB,Van Dyke AL,Land SJ,Schwartz AG

    更新日期:2009-04-01 00:00:00

  • Role of the maternal environment in determining susceptibility to transplacentally induced chemical carcinogenesis in mouse fetuses.

    abstract::Treatment of pregnant mice with 3-methylcholanthrene (MC) causes lung and liver tumors in the offspring, the incidences of which are greatly influenced by the Ah locus regulated induction phenotype for aryl hydrocarbon hydroxylase activity (AHH) in both the mother and fetuses. In order to examine the biochemical and m...


    pub_type: 杂志文章


    authors: Miller MS,Jones AB,Chauhan DP,Anderson LM

    更新日期:1990-11-01 00:00:00