The use of initiated cells as a test system for the detection of inhibitors of gap junctional intercellular communication.


:The effects of five non-mutagenic carcinogens--Aroclor 1260, benzoyl peroxide (BP), phenobarbital (PB), 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1,1'-(2,2,2-trichloroethylidene)bis[4-chlorobenzene] (DDT)--on gap junctional intercellular communication (GJIC) were tested in a cell line consisting of initiated cells (3PC). Four agents suspected of tumor promotion activity--o-anisidine, clofibrate, L-ethionone and d-limonene--were also tested for their effects on GJIC. Finally sodium fluoride (NaF), whose carcinogenic property is still unclear, was tested for its effects on GJIC in the 3PC cell line. Four of the five selected tumor promoters (Aroclor 1260, BP, DDT and TPA) decreased GJIC between these initiated epidermal cells. The four non-mutagenic carcinogens with tumor-promoting activity in vivo (o-anisidine, clofibrate, L-ethionine and d-limonene) all inhibited GJIC, whereas NaF had no effect. Seven compounds (o-anisidine, Aroclor 1260, BP, DDT, L-ethionine, d-limonene and TPA) had a dose-dependent as well as time-dependent inhibitory effect on GJIC. Under the experimental conditions used, clofibrate showed only a dose-related inhibition of GJIC. PB showed no inhibitory effect on GJIC in the 3PC cell line. In order to determine the role of biotransformation in the tumor-promoting activity of PB, its effect on GJIC was also examined in the presence of an Aroclor 1254-induced rat liver homogenate (S9 mix) and in the hepatoma cell line HepG2. In the presence of rat liver homogenate PB decreased GJIC in the 3PC cell line, whereas in the HepG2 cells PB showed a time- and dose-dependent inhibitory effect. To study the potential differences in susceptibility of cells representing different stages in the process of tumor formation, the effect of the selected tumor promoters on GJIC was also investigated in primary mouse keratinocytes and in a mouse skin carcinoma-derived cell line (CA3/7). Primary keratinocytes were sometimes more (BP and clofibrate) and sometimes less sensitive (ethionine and limonene) for inhibitory effects on GJIC compared to the effects in the cell line 3PC. Except for TPA and anisidin, GJIC between the CA3/7 cells was less affected by the selected agents compared to the 3PC cell line. These results show that, during the process of tumor formation the susceptibility of cells to inhibition of GJIC by tumor promoters is variable. Overall the CA3/7 cells are less sensitive compared to 3PC cells. The susceptibility of primary keratinocytes is variable compared to 3PC cells, depending on the agent used. These results also show that GJIC is a valid parameter for testing the tumor-promoting activity of compounds. Finally, this study demonstrates that mouse keratinocyte cell lines could serve as an in vitro model for the detection of non-mutagenic carcinogens with diverse target organs in vivo. For this use the cell line consisting of initiated cells (3PC) is more sensitive than the carcinoma-derived cell line CA3/7.






Jansen LA,Jongen WM




Has Abstract


1996-02-01 00:00:00












  • Influence of DNA structure on hypoxanthine and 1,N(6)-ethenoadenine removal by murine 3-methyladenine DNA glycosylase.

    abstract::3-Methyladenine DNA glycosylases initiate base excision repair by flipping the nucleotide bearing the target base out of double-stranded DNA into an active site pocket for glycosylic bond cleavage and base release. Substrate bases for the murine 3-methyladenine DNA glycosylase (other than 3-methyladenine) include hypo...


    pub_type: 杂志文章


    authors: Wyatt MD,Samson LD

    更新日期:2000-05-01 00:00:00

  • Effect of cAMP elevating compounds on inhibition of gap junctional communication and induction of morphological transformation in Syrian hamster embryo cells.

    abstract::An enhancement of the cellular cAMP level has been shown to protect against phorbol ester-induced inhibition of gap junctional intercellular communication (GJIC) and induction of morphological transformation in Syrian hamster embryo (SHE) cells. Cholera toxin, forskolin, 3-isobutyl-1-methylxanthine (IBMX) and theophyl...


    pub_type: 杂志文章


    authors: Roseng LE,Rivedal E,Sanner T

    更新日期:1992-10-01 00:00:00

  • Myeloperoxidase-enhanced formation of (+-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene-DNA adducts in lung tissue in vitro: a role of pulmonary inflammation in the bioactivation of a procarcinogen.

    abstract::Several studies have indicated a correlation between the presence of inflammation and the development of cancer. The aim of our study was to determine if pulmonary neutrophils could transform the proximate respiratory carcinogen (+-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (B[a]P-7,8-diol), to an ultimate carcin...


    pub_type: 杂志文章


    authors: Petruska JM,Mosebrook DR,Jakab GJ,Trush MA

    更新日期:1992-07-01 00:00:00

  • Clove (Syzygium aromaticum L.), a potential chemopreventive agent for lung cancer.

    abstract::Spices and flavoring plants part rich in supposedly health-promoting phytochemicals are currently receiving much attention as a possible source of cancer chemopreventive compounds. Clove, the sun-dried unopened flower bud from the plant Syzygium aromaticum L. is a commonly used spice and food flavor. In the present wo...


    pub_type: 杂志文章


    authors: Banerjee S,Panda CK,Das S

    更新日期:2006-08-01 00:00:00

  • Contribution of the orphan nuclear receptor Nur77 to the apoptotic action of IGFBP-3.

    abstract::Tumor suppression by insulin-like growth factor-binding protein-3 (IGFBP-3) has been demonstrated to occur via insulin-like growth factor-dependent and -independent mechanisms in vitro and in vivo. We have recently described IGFBP-3-induced mitochondrial translocation of the nuclear receptors RXRalpha/Nur77 in the ind...


    pub_type: 杂志文章


    authors: Lee KW,Cobb LJ,Paharkova-Vatchkova V,Liu B,Milbrandt J,Cohen P

    更新日期:2007-08-01 00:00:00

  • Basic properties and molecular mechanisms of exogenous chemical carcinogens.

    abstract::Exogenous chemical carcinogenesis is an extremely complex multifactorial process during which gene-environment interactions involving chronic exposure to exogenous chemical carcinogens (ECCs) and polymorphisms of cancer susceptibility genes add further complexity. We describe the properties and molecular mechanisms of...


    pub_type: 杂志文章,评审


    authors: Irigaray P,Belpomme D

    更新日期:2010-02-01 00:00:00

  • YAP is a candidate oncogene for esophageal squamous cell carcinoma.

    abstract::Yes-associated protein (YAP), the nuclear effector of the Hippo pathway, is a key regulator of organ size and a candidate human oncogene located at chromosome 11q22. Since we previously reported amplification of 11q22 region in esophageal squamous cell carcinoma (ESCC), in this study we focused on the clinical signifi...


    pub_type: 杂志文章


    authors: Muramatsu T,Imoto I,Matsui T,Kozaki K,Haruki S,Sudol M,Shimada Y,Tsuda H,Kawano T,Inazawa J

    更新日期:2011-03-01 00:00:00

  • Ionomycin downregulates beta-catenin/Tcf signaling in colon cancer cell line.

    abstract::Functional activation of beta-catenin/Tcf signaling plays an important role in the early events in colorectal carcinogenesis. We examined the effect of ionomycin against beta-catenin/Tcf signaling in colon cancer cells. Reporter gene assay showed that ionomycin inhibited beta-catenin/Tcf signaling efficiently. In addi...


    pub_type: 杂志文章


    authors: Park CH,Hahm ER,Lee JH,Jung KC,Rhee HS,Yang CH

    更新日期:2005-11-01 00:00:00

  • Dose-related differences in DNA adduct levels in rodent tissues following skin application of complex mixtures from air pollution sources.

    abstract::Dose-related differences in the binding of DNA reactive intermediates for three environmentally important complex mixture particulate extracts and a well-studied carcinogen, benzo[a]pyrene (BaP), were examined in female C-57 mice following multiple topical treatments ranging from 1 to 120 mg/mouse. Particulate extract...


    pub_type: 杂志文章


    authors: Gallagher JE,Jackson MA,George MH,Lewtas J

    更新日期:1990-01-01 00:00:00

  • Selective changes of retroelement expression in human prostate cancer.

    abstract::Retroelements constitute a large part of the human genome. These sequences are mostly silenced in normal cells, but genome-wide DNA hypomethylation in cancers might lead to their re-expression. Whether this re-expression really occurs in human cancers is largely unkown. We therefore investigated expression and DNA met...


    pub_type: 杂志文章


    authors: Goering W,Ribarska T,Schulz WA

    更新日期:2011-10-01 00:00:00

  • DNA polymerase action on an oligonucleotide containing a site-specifically located N-(deoxyguanosin-8-yl)-1-aminopyrene.

    abstract::A 25mer oligonucleotide containing a single N-(deoxyguanosin-8-yl-)-1-aminopyrene (dGAP), the major DNA adduct formed by reductively activated 1-nitropyrene, was synthesized. The adduct was located at nucleotide 21 from the 3' end. DNA synthesis on this template by human DNA polymerases alpha and beta, HIV reverse tra...


    pub_type: 杂志文章


    authors: Vyas RR,Basu AK

    更新日期:1995-04-01 00:00:00

  • Ellagic acid induces transcription of the rat glutathione S-transferase-Ya gene.

    abstract::Induction of glutathione S-transferase (GST) enzymes can increase detoxification of carcinogens and reduce carcinogen-induced mutagenesis and tumorigenesis. To determine if the anticarcinogen ellagic acid induces cellular enzymes which detoxify carcinogens, we examined the effect of ellagic acid on the expression of g...


    pub_type: 杂志文章


    authors: Barch DH,Rundhaugen LM,Pillay NS

    更新日期:1995-03-01 00:00:00

  • Evidence that weak promotion of carcinogen-initiated cells prevents their progression to malignancy.

    abstract::Repeated promotion of 7,12-dimethylbenz[alpha]anthracene-initiated cells in mouse skin with 12-O-tetradecanoylphorbol-13-acetate (TPA) induces them to grow as premalignant skin papillomas and some of these subsequently progress to carcinomas. In this study, we demonstrate that this TPA-induced progression of initiated...


    pub_type: 杂志文章


    authors: Reddy AL,Fialkow PJ

    更新日期:1990-12-01 00:00:00

  • Instability of (+/-)-7 beta,8 alpha-dihydroxy-9 beta,10 beta-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (syn-BaPDE)-DNA adducts formed in benzo[a]pyrene-treated Wistar rat embryo cell cultures.

    abstract::One of the peaks present in HPLC profiles of [3H]benzo[a]-pyrene (BaP)-deoxyribonucleosides prepared by enzymatic degradation of [3H]BaP-DNA isolated from Wistar rat embryo cell cultures exposed to [G-3H]BaP was found to be r-7,c-9,c-10,t-8-tetrahydroxy-7,8,9,10-tetrahydroBaP, a BaP-DNA adduct decomposition product (P...


    pub_type: 杂志文章


    authors: Pruess-Schwartz D,Mauthe RJ,Baird WM

    更新日期:1988-10-01 00:00:00

  • Effects of testosterone, testosterone propionate, 17 beta-trenbolone and progesterone on cell transformation and mutagenesis in Syrian hamster embryo cells.

    abstract::Testosterone, testosterone propionate, 17 beta-trenbolone and progesterone, which represent the main endogenous and synthetic androgens and a progestin, were evaluated for possible cell transformation and genetic effects in Syrian hamster embryo (SHE) cells. Cell growth was reduced by treatment with the steroids at 10...


    pub_type: 杂志文章


    authors: Tsutsui T,Komine A,Huff J,Barrett JC

    更新日期:1995-06-01 00:00:00

  • Inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone carcinogenesis in mice by D-limonene and citrus fruit oils.

    abstract::The effects of D-limonene and citrus fruit oils, i.e. orange oil and lemon oil, on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced neoplasia of the lungs and forestomach of female A/J mice were investigated. D-Limonene and the citrus fruit oils given p.o. 1 h prior to NNK, also administered p.o., inhibite...


    pub_type: 杂志文章


    authors: Wattenberg LW,Coccia JB

    更新日期:1991-01-01 00:00:00

  • Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes.

    abstract::Ovarian surface epithelium (OSE) is considered to give rise to epithelial ovarian carcinomas (EOCs). To elucidate early processes contributing to the development of EOCs from the OSE, two batches of primary human OSE cells were transduced with non-viral human genes (mutant Cdk4, cyclinD1 and hTERT) so as to efficientl...


    pub_type: 杂志文章


    authors: Sasaki R,Narisawa-Saito M,Yugawa T,Fujita M,Tashiro H,Katabuchi H,Kiyono T

    更新日期:2009-03-01 00:00:00

  • Synergistic induction of DNA strand breakage by cigarette tar and nitric oxide.

    abstract::Cigarette smoking is a major cause of human cancer at a variety of sites, although its carcinogenic mechanisms remains unestablished. Cigarette smoke can be divided into two phases, gas phase and particulate matter (tar). Both phases contain high concentrations of oxidants and free radicals, especially nitric oxide (N...


    pub_type: 杂志文章


    authors: Yoshie Y,Ohshima H

    更新日期:1997-07-01 00:00:00

  • All-trans and 9-cis retinoic acids, retinol and beta-carotene chemopreventive activities during the initial phases of hepatocarcinogenesis involve distinct actions on glutathione S-transferase positive preneoplastic lesions remodeling and DNA damage.

    abstract::Chemopreventive activities of all-trans retinoic acid (AtRA), 9-cis retinoic acid (9cRA), retinol (ROL) and beta-carotene (betaC) were evaluated during hepatocarcinogenesis. Rats received 1 mg/100 g body wt AtRA (AtRA group), 9cRA (9cRA group), ROL (ROL group), 7 mg/100 g body wt betaC (betaC group) or corn oil (CO gr...


    pub_type: 杂志文章


    authors: de Almeida Vasconcelos Fonseca EM,Chagas CE,Mazzantini RP,Heidor R,Ong TP,Moreno FS

    更新日期:2005-11-01 00:00:00

  • The coordinate regulation of DNA synthesis and suppression of apoptosis is differentially regulated by the liver growth agents, phenobarbital and methylclofenapate.

    abstract::The coordinate regulation of DNA synthesis and suppression of apoptosis was investigated in a rat hepatocyte cell culture system which supports high level induction of DNA synthesis by the peroxisome proliferator, methylclofenapate (MCP) (Plant, N.J. et al., 1998, Carcinogenesis, 19, 925-931). The peroxisome prolifera...


    pub_type: 杂志文章


    authors: Plant NJ,Horley NJ,Dickins M,Hasmall S,Elcombe CR,Bell DR

    更新日期:1998-09-01 00:00:00

  • Hepatic and extrahepatic bioactivation and GSH conjugation of aflatoxin B1 in sheep.

    abstract::Whole-body autoradiography of [3H]aflatoxin B1 ([3H]AFB1) in lamb showed a localization of bound labelling, in addition to the liver, in the nasal olfactory and respiratory mucosa, in the mucosa of the nasopharynx, pharynx, oesophagus, larynx, trachea, bronchi and bronchioles and in the palpebral and bulbar conjunctiv...


    pub_type: 杂志文章


    authors: Larsson P,Busk L,Tjälve H

    更新日期:1994-05-01 00:00:00

  • Characterization of neutral metabolites of benzo[a]pyrene in urine from germfree rats.

    abstract::[14C]Benzo[a]pyrene (BP) was administered to male germfree rats. Urinary metabolites, constituting 9% of the administered radioactivity, were fractionated by lipophilic ion exchange chromatography. More than 80% of the urinary metabolites were conjugated, while neutral metabolites constituted 13-18%. The latter group ...


    pub_type: 杂志文章


    authors: Yang Y,Sjövall J,Rafter J,Gustafsson JA

    更新日期:1994-04-01 00:00:00

  • U.v.-induced DNA damage and its repair in human skin in vivo studied by sensitive immunohistochemical methods.

    abstract::Antibodies specific for u.v.-induced DNA damage were raised in rabbits, and used to study damage and repair of nuclear DNA in nude mouse and human skin in vivo by immuno-fluorescence and immunoperoxidase techniques. Purification of the antibodies by affinity chromatography strongly reduced unspecific background staini...


    pub_type: 杂志文章


    authors: Eggset G,Volden G,Krokan H

    更新日期:1983-01-01 00:00:00

  • Two-stage skin carcinogenesis in sensitive and resistant mouse strains.

    abstract::Strains of laboratory mice vary markedly in their susceptibility to two-stage skin carcinogenesis using 7,12-dimethylbenz[a]anthracene (DMBA) as the initiating carcinogen and croton oil as promoter. This study has been undertaken in order to clarify the basis of the strain differences. LACA mice were used as the susce...


    pub_type: 杂志文章


    authors: Ashman LK,Murray AW,Cook MG,Kotlarski I

    更新日期:1982-01-01 00:00:00

  • Transformation of immortalized woodchuck hepatic cell lines with the c-Ha-ras proto-oncogene.

    abstract::Woodchuck hepatocytes were immortalized with the simian virus 40 T antigen (SV40 T-ag) oncogene and utilized in an oncogenic transformation assay. Transfection of these cell lines with an activated C-Ha-ras oncogene (EJ6.6) resulted in the transformation of cells to a phenotype characterized by anchorage-independent g...


    pub_type: 杂志文章


    authors: Jacob JR,Tennant BC

    更新日期:1996-04-01 00:00:00

  • Induction by asbestos fibers of anaphase abnormalities: mechanism for aneuploidy induction and possibly carcinogenesis.

    abstract::Syrian hamster embryo cells were treated with crocidolite asbestos at a dose (1 microgram/cm2) which was shown in previous studies to induce cell transformation and aneuploidy in these cells. Treatment of cells with asbestos induced a greater than 20-fold increase in the incidence of cells in anaphase with abnormaliti...


    pub_type: 杂志文章


    authors: Hesterberg TW,Barrett JC

    更新日期:1985-03-01 00:00:00

  • Genetic variants in the calcium signaling pathway genes are associated with cutaneous melanoma-specific survival.

    abstract::Remodeling or deregulation of the calcium signaling pathway is a relevant hallmark of cancer including cutaneous melanoma (CM). In this study, using data from a published genome-wide association study (GWAS) from The University of Texas M.D. Anderson Cancer Center, we assessed the role of 41,377 common single-nucleoti...


    pub_type: 杂志文章


    authors: Wang X,Liu H,Xu Y,Xie J,Zhu D,Amos CI,Fang S,Lee JE,Li X,Nan H,Song Y,Wei Q

    更新日期:2019-04-29 00:00:00

  • Reactivity of benzidine diimine with DNA to form N-(deoxyguanosin-8-yl)-benzidine.

    abstract::Benzidine diimine (BZDI), a reactive intermediate in the metabolic peroxidation of the carcinogen benzidine, has been reported to bind covalently to cellular proteins and nucleic acids. We have examined the nature of this interaction with DNA and have identified a major carcinogen-nucleoside adduct. BZDI (20-50 microM...


    pub_type: 杂志文章


    authors: Yamazoe Y,Roth RW,Kadlubar FF

    更新日期:1986-01-01 00:00:00

  • Admixture mapping of lung cancer in 1812 African-Americans.

    abstract::Lung cancer continues to be the leading cause of cancer death in the USA and the best example of a cancer with undisputed evidence of environmental risk. However, a genetic contribution to lung cancer has also been demonstrated by studies of familial aggregation, family-based linkage, candidate gene studies and most r...


    pub_type: 杂志文章


    authors: Schwartz AG,Wenzlaff AS,Bock CH,Ruterbusch JJ,Chen W,Cote ML,Artis AS,Van Dyke AL,Land SJ,Harris CC,Pine SR,Spitz MR,Amos CI,Levin AM,McKeigue PM

    更新日期:2011-03-01 00:00:00

  • DNA polymerase, RNA polymerase and exonuclease activities on a DNA sequence modified by benzo[a]pyrene diolepoxide.

    abstract::Adducts produced by modification of DNA with benzo[a]pyrene diolepoxide (BPDE) are known to inhibit both DNA and RNA synthesis. This phenomenon has been used as a method for determining the distribution of carcinogen binding within defined DNA sequences. A critical comparison of different enzyme activities on adducted...


    pub_type: 杂志文章


    authors: Thrall BD,Mann DB,Smerdon MJ,Springer DL

    更新日期:1992-09-01 00:00:00