The use of initiated cells as a test system for the detection of inhibitors of gap junctional intercellular communication.


:The effects of five non-mutagenic carcinogens--Aroclor 1260, benzoyl peroxide (BP), phenobarbital (PB), 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1,1'-(2,2,2-trichloroethylidene)bis[4-chlorobenzene] (DDT)--on gap junctional intercellular communication (GJIC) were tested in a cell line consisting of initiated cells (3PC). Four agents suspected of tumor promotion activity--o-anisidine, clofibrate, L-ethionone and d-limonene--were also tested for their effects on GJIC. Finally sodium fluoride (NaF), whose carcinogenic property is still unclear, was tested for its effects on GJIC in the 3PC cell line. Four of the five selected tumor promoters (Aroclor 1260, BP, DDT and TPA) decreased GJIC between these initiated epidermal cells. The four non-mutagenic carcinogens with tumor-promoting activity in vivo (o-anisidine, clofibrate, L-ethionine and d-limonene) all inhibited GJIC, whereas NaF had no effect. Seven compounds (o-anisidine, Aroclor 1260, BP, DDT, L-ethionine, d-limonene and TPA) had a dose-dependent as well as time-dependent inhibitory effect on GJIC. Under the experimental conditions used, clofibrate showed only a dose-related inhibition of GJIC. PB showed no inhibitory effect on GJIC in the 3PC cell line. In order to determine the role of biotransformation in the tumor-promoting activity of PB, its effect on GJIC was also examined in the presence of an Aroclor 1254-induced rat liver homogenate (S9 mix) and in the hepatoma cell line HepG2. In the presence of rat liver homogenate PB decreased GJIC in the 3PC cell line, whereas in the HepG2 cells PB showed a time- and dose-dependent inhibitory effect. To study the potential differences in susceptibility of cells representing different stages in the process of tumor formation, the effect of the selected tumor promoters on GJIC was also investigated in primary mouse keratinocytes and in a mouse skin carcinoma-derived cell line (CA3/7). Primary keratinocytes were sometimes more (BP and clofibrate) and sometimes less sensitive (ethionine and limonene) for inhibitory effects on GJIC compared to the effects in the cell line 3PC. Except for TPA and anisidin, GJIC between the CA3/7 cells was less affected by the selected agents compared to the 3PC cell line. These results show that, during the process of tumor formation the susceptibility of cells to inhibition of GJIC by tumor promoters is variable. Overall the CA3/7 cells are less sensitive compared to 3PC cells. The susceptibility of primary keratinocytes is variable compared to 3PC cells, depending on the agent used. These results also show that GJIC is a valid parameter for testing the tumor-promoting activity of compounds. Finally, this study demonstrates that mouse keratinocyte cell lines could serve as an in vitro model for the detection of non-mutagenic carcinogens with diverse target organs in vivo. For this use the cell line consisting of initiated cells (3PC) is more sensitive than the carcinoma-derived cell line CA3/7.






Jansen LA,Jongen WM




Has Abstract


1996-02-01 00:00:00












  • Measurement and characterization of the denitrosation of tauromustine and related nitrosoureas by glutathione transferases in liver cytosol from various species.

    abstract::The denitrosation of a novel nitrosourea, 1-(2-chloroethyl)-3-[(2-dimethylaminosulfonyl)-ethyl]-1-nitrosoure a, tauromustine (TCNU), has been investigated in liver cytosol from various species and with a pool of cytosolic rat liver glutathione transferases (GSTs). In addition, the denitrosation of related nitrosoureas...


    pub_type: 杂志文章


    authors: Tuvesson H,Gunnarsson PO,Seidegård J

    更新日期:1993-06-01 00:00:00

  • Dependence of u.v.-induced DNA excision repair on deoxyribonucleoside triphosphate concentrations in permeable human fibroblasts: a model for the inhibition of repair by hydroxyurea.

    abstract::We have tested the hypothesis that the inhibition by hydroxyurea of repair patch ligation and chromatin rearrangement during u.v.-induced DNA excision repair results from a reduction in cellular deoxyribonucleotide concentrations and not from a direct effect of hydroxyurea on the repair process. Using permeable human ...


    pub_type: 杂志文章


    authors: Hunting DJ,Dresler SL

    更新日期:1985-10-01 00:00:00

  • 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: effects of sequence contexts on mutation spectra.

    abstract::We previously reported that 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) at the second position of codon 12 of the c-Ha-ras gene induces many types of mutations in NIH3T3 cells. In this study we incorporated the modified base into the first and second positions of codon 12 in the coding strand and into the first positi...


    pub_type: 杂志文章


    authors: Kamiya H,Murata-Kamiya N,Koizume S,Inoue H,Nishimura S,Ohtsuka E

    更新日期:1995-04-01 00:00:00

  • Peroxisome proliferation and modulation of rat liver carcinogenesis by 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, perfluorooctanoic acid and nafenopin.

    abstract::Using an initiation--selection--promotion protocol for induction of liver tumors in Wistar rats, the modulating action of various peroxisome proliferators on neoplasia as well as on selected biochemical parameters was studied. After treatment with diethylnitrosamine (DEN), the animals were subsequently subjected to a ...


    pub_type: 杂志文章


    authors: Abdellatif AG,Préat V,Vamecq J,Nilsson R,Roberfroid M

    更新日期:1990-11-01 00:00:00

  • U.v.-induced DNA damage and its repair in human skin in vivo studied by sensitive immunohistochemical methods.

    abstract::Antibodies specific for u.v.-induced DNA damage were raised in rabbits, and used to study damage and repair of nuclear DNA in nude mouse and human skin in vivo by immuno-fluorescence and immunoperoxidase techniques. Purification of the antibodies by affinity chromatography strongly reduced unspecific background staini...


    pub_type: 杂志文章


    authors: Eggset G,Volden G,Krokan H

    更新日期:1983-01-01 00:00:00

  • Comparative benefits of Nab-paclitaxel over gemcitabine or polysorbate-based docetaxel in experimental pancreatic cancer.

    abstract::Gemcitabine has limited clinical benefits in pancreatic ductal adenocarcinoma. The solvent-based traditional taxanes docetaxel and paclitaxel have not shown clinical results superior to gemcitabine. Nab-paclitaxel, a water-soluble albumin-bound paclitaxel, may carry superior distribution properties into the tumor micr...


    pub_type: 杂志文章


    authors: Awasthi N,Zhang C,Schwarz AM,Hinz S,Wang C,Williams NS,Schwarz MA,Schwarz RE

    更新日期:2013-10-01 00:00:00

  • Cancer stemness in Wnt-driven mammary tumorigenesis.

    abstract::Wnt signaling plays a central role in mammary stem cell (MaSC) homeostasis and in breast cancer. In particular, epigenetic alterations at different members of the Wnt pathway have been identified among triple-negative, basal-like breast cancers. Previously, we developed a mouse model for metaplastic breast adenocarcin...


    pub_type: 杂志文章


    authors: Monteiro J,Gaspar C,Richer W,Franken PF,Sacchetti A,Joosten R,Idali A,Brandao J,Decraene C,Fodde R

    更新日期:2014-01-01 00:00:00

  • Calcium ameliorates the toxic effect of deoxycholic acid on colonic epithelium.

    abstract::The effect of a deoxycholic acid instillation on colonic epithelium was investigated and compared with the effect of an intervention scheme in which deoxycholic acid (DCA) treated mice received oral supplements of calcium lactate. The morphology of colonic tissue exposed to DCA was markedly affected when compared to t...


    pub_type: 杂志文章


    authors: Wargovich MJ,Eng VW,Newmark HL,Bruce WR

    更新日期:1983-09-01 00:00:00

  • The redox protein thioredoxin-1 regulates the constitutive and inducible expression of the estrogen metabolizing cytochromes P450 1B1 and 1A1 in MCF-7 human breast cancer cells.

    abstract::The oxidative metabolites of estrogen have been proposed to play an important role in the development of some human cancers. The two major pathways of estrogen metabolism, to the carcinogenic 4-hydroxyestradiol (4-OHE2) and to the non-carcinogenic 2-hydroxyestradiol (2-OHE2), are mediated by cytochromes P450 CYP1B1 an...


    pub_type: 杂志文章


    authors: Husbeck B,Powis G

    更新日期:2002-10-01 00:00:00

  • Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.

    abstract::Tamoxifen is a non-steroidal anti-estrogen used for the treatment of breast cancer and, more recently, as a chemopreventive agent in healthy women at high risk of developing breast cancer. On the other hand, tamoxifen is a potent hepatocarcinogen in rats, with both tumor-initiating and tumor-promoting properties. Ther...


    pub_type: 杂志文章


    authors: Tryndyak VP,Muskhelishvili L,Kovalchuk O,Rodriguez-Juarez R,Montgomery B,Churchwell MI,Ross SA,Beland FA,Pogribny IP

    更新日期:2006-08-01 00:00:00

  • Reduced expression of EphB2 that parallels invasion and metastasis in colorectal tumours.

    abstract::EphB2, a receptor tyrosine kinase regulated by the beta-catenin/Tcf4 complex, is expressed in the proliferative compartment of mouse intestine and regulates bidirectional migration of intestinal precursor cells in the crypt-villus axis through repulsive interaction with Ephrin-B ligands. Recently, it has been shown th...


    pub_type: 杂志文章


    authors: Guo DL,Zhang J,Yuen ST,Tsui WY,Chan AS,Ho C,Ji J,Leung SY,Chen X

    更新日期:2006-03-01 00:00:00

  • Comparison of mutagenesis by O6-methyl- and O6-ethylguanine and O4-methylthymine in Escherichia coli using double-stranded and gapped plasmids.

    abstract::To compare mutagenesis by O6-methylguanine (m6G), O4-methylthymine (m4T) and O6-ethylguanine (e6G), and assess their genotoxicity in Escherichia coli, double-stranded and gapped plasmids were constructed containing a single m6G, e6G or m4T in the initiation codon (ATG) of a lacZ' gene. Modified base induced mutations ...


    pub_type: 杂志文章


    authors: Pauly GT,Hughes SH,Moschel RC

    更新日期:1998-03-01 00:00:00

  • Non-codingRNA sequence variations in human chronic lymphocytic leukemia and colorectal cancer.

    abstract::Cancer is a genetic disease in which the interplay between alterations in protein-coding genes and non-coding RNAs (ncRNAs) plays a fundamental role. In recent years, the full coding component of the human genome was sequenced in various cancers, whereas such attempts related to ncRNAs are still fragmentary. We screen...


    pub_type: 杂志文章


    authors: Wojcik SE,Rossi S,Shimizu M,Nicoloso MS,Cimmino A,Alder H,Herlea V,Rassenti LZ,Rai KR,Kipps TJ,Keating MJ,Croce CM,Calin GA

    更新日期:2010-02-01 00:00:00

  • Dose-related differences in DNA adduct levels in rodent tissues following skin application of complex mixtures from air pollution sources.

    abstract::Dose-related differences in the binding of DNA reactive intermediates for three environmentally important complex mixture particulate extracts and a well-studied carcinogen, benzo[a]pyrene (BaP), were examined in female C-57 mice following multiple topical treatments ranging from 1 to 120 mg/mouse. Particulate extract...


    pub_type: 杂志文章


    authors: Gallagher JE,Jackson MA,George MH,Lewtas J

    更新日期:1990-01-01 00:00:00

  • Transforming activity of the human mammary line HBL100 DNA is associated with SV40 large T antigen genetic information integrated in its genome.

    abstract::The HBL100 cell line, established in vitro from milk of an apparently healthy woman, expresses SV40 large T antigen defective in some of its functions. Here we demonstrate that the HBL100 genomic DNA possesses transforming activity in NIH3T3 cells and that this activity is associated with the viral information. ...


    pub_type: 杂志文章


    authors: Vanhamme L,Szpirer C

    更新日期:1988-04-01 00:00:00

  • An insulin-like growth factor-II intronic variant affects local DNA conformation and ovarian cancer survival.

    abstract::Insulin-like growth factor-II (IGF-II) may be a prognostic marker in ovarian cancer, and its intronic single nucleotide polymorphism (SNP) rs4320932 has been associated with risk of the disease. We determined whether rs4320932 is associated with IGF-II expression and patient survival in ovarian cancer, and explored wh...


    pub_type: 杂志文章


    authors: Lu L,Risch E,Deng Q,Biglia N,Picardo E,Katsaros D,Yu H

    更新日期:2013-09-01 00:00:00

  • Genomic pattern of intratumor heterogeneity predicts the risk of progression in early stage diffuse large B-cell lymphoma.

    abstract::Intratumor heterogeneity (ITH) plays an important role in tumor development, metastases, recurrence and has impact on clinical diagnosis, therapeutic responses. In this study, we used mutant-allele tumor heterogeneity (MATH) through whole-exome sequencing data to evaluate ITH in early stage diffuse large B-cell lympho...


    pub_type: 杂志文章


    authors: Wang Y,Feng W,Liu P

    更新日期:2019-11-25 00:00:00

  • Comparison of hydrocarbon-DNA adducts formed in mouse skin in vivo and in organ culture in vitro following treatment with benzo[a]pyrene.

    abstract::Species differences in the metabolism of toxic chemicals can confound the extrapolation of experimentally determined risk data to man. However, it is often difficult to obtain reliable information on human metabolism, particularly of genotoxic agents. In this study, comparisons of chromatographic profiles of DNA adduc...


    pub_type: 杂志文章


    authors: Huckle KR,Smith RJ,Watson WP,Wright AS

    更新日期:1986-06-01 00:00:00

  • The yin and yang of nitric oxide in cancer progression.

    abstract::Nitric oxide (NO) is a short-lived, pleiotropic molecule that affects numerous critical functions in the body. Presently, there are markedly conflicting findings in the literature regarding NO and its role in carcinogenesis and tumor progression. NO has been shown to have dichotomous effects on cellular proliferation,...


    pub_type: 杂志文章,评审


    authors: Burke AJ,Sullivan FJ,Giles FJ,Glynn SA

    更新日期:2013-03-01 00:00:00

  • Emergence of undifferentiated rat tracheal cell carcinomas, but not squamous cell carcinomas, is associated with a loss of expression of E-cadherin and of gap junction communication.

    abstract::A series of cells representing normal, non-tumorigenic cell lines, as well as differentiating neoplastic and undifferentiated neoplastic rat tracheal epithelial cell populations were evaluated for their ability to establish homologous and/or heterologous cell-cell gap junction communication in culture. Gap junction co...


    pub_type: 杂志文章


    authors: Terzaghi-Howe M,Chang GW,Popp D

    更新日期:1997-11-01 00:00:00

  • Possible anti-tumour-promoting activity of components in Japanese soybean fermented food, Natto: effect on gap junctional intercellular communication.

    abstract::In order to detect any protective agent against tumor formation, we examined the anti-tumor-promoting effect of a Japanese traditional soybean fermented food, Natto. Dye transfer was employed as an assay method. When fluorescent dye was microinjected into cultured BALB/3T3 cells, the dye was transformed into the neigh...


    pub_type: 杂志文章


    authors: Takahashi C,Kikuchi N,Katou N,Miki T,Yanagida F,Umeda M

    更新日期:1995-03-01 00:00:00

  • Cell specificity of rat cytokeratin p39 during azo dye-induced hepatocarcinogenesis.

    abstract::Monoclonal or affinity-purified antibodies specific to Novikoff hepatoma cytokeratin p39 were employed to study the origin and fate of p39-containing cell types during hepatocarcinogenesis induced with N,N-dimethyl-p(m-tolylazo)aniline. Frozen sections were obtained from the livers of animals autopsied temporally duri...


    pub_type: 杂志文章


    authors: Schmidt WN,Page DL,McKusick K,Hnilica LS

    更新日期:1985-08-01 00:00:00

  • Epidermal growth factor, like tumor promoters, enhances viral and radiation-induced cell transformation.

    abstract::The polypeptide hormone epidermal growth factor (EGF) has been shown to enhance adenovirus type 5 transformation of a cloned culture of rat embryo cells (CREF) and X-ray or u.v.-light induced transformation of 10T1/2 mouse embryo cells. In both systems, the degree of enhancement was quantitatively similar to that obse...


    pub_type: 杂志文章


    authors: Fisher PB,Mufson RA,Weinstein IB,Little JB

    更新日期:1981-01-01 00:00:00

  • CYP1A1 and GSTM1 genotypes affect benzo[a]pyrene DNA adducts in smokers' lung: comparison with aromatic/hydrophobic adduct formation.

    abstract::Benzo[a]pyrene diol epoxide (BPDE)-DNA adducts are involved in the induction of p53 mutations and probably in the causation of human lung cancer associated with cigarette smoking. The ratio between CYP1A1 and GST enzyme activities is a critical determinant of the target dose of carcinogenic BPDE and other DNA-reactive...


    pub_type: 杂志文章,评审


    authors: Alexandrov K,Cascorbi I,Rojas M,Bouvier G,Kriek E,Bartsch H

    更新日期:2002-12-01 00:00:00

  • Genetic differences in the enzymic properties of the aromatic hydrocarbon inducible N-hydroxylation of 2-acetylaminofluorene in mouse liver.

    abstract::3-Methylcholanthrene (3-MC) pretreatment induces the liver microsomal 2-acetylaminofluorene (2-AAF) N-hydroxylase activity of C57BL6 responsive mice. The same pretreatment modifies this enzyme by significantly increasing its apparent Km value which, moreover, becomes protein concentration dependent. After 3-MC inducti...


    pub_type: 杂志文章


    authors: Razzouk C,Batardy-Grégoire M,Roberfroid M

    更新日期:1982-01-01 00:00:00

  • Repair of lethal, mutagenic and transforming damage induced by X-rays in golden hamster embryo cells.

    abstract::We compared the frequencies of mutation and morphological transformation in relation to the repair of potentially lethal damage (PLD) and sublethal damage (SLD) in X-irradiated golden hamster embryo (GHE) cells. The repair of PLD induced in GHE cells by X-rays was shown to be an error-free process which reduced the fr...


    pub_type: 杂志文章


    authors: Watanabe M,Suzuki N,Sawada S,Nikaido O

    更新日期:1984-10-01 00:00:00

  • Read-through transcripts in lung: germline genetic regulation and correlation with the expression of other genes.

    abstract::Transcripts originating from the transcriptional read through of two adjacent, similarly oriented genes have been identified in normal and neoplastic tissues, but their functional role and the mechanisms that regulate their expression are mostly unknown. Here, we investigated whether the expression of read-through tra...


    pub_type: 杂志文章


    authors: Maspero D,Dassano A,Pintarelli G,Noci S,De Cecco L,Incarbone M,Tosi D,Santambrogio L,Dragani TA,Colombo F

    更新日期:2020-07-14 00:00:00

  • Deficiency of tumor suppressor Merlin facilitates metabolic adaptation by co-operative engagement of SMAD-Hippo signaling in breast cancer.

    abstract::The NF2 gene encodes the tumor and metastasis suppressor protein Merlin. Merlin exerts its tumor suppressive role by inhibiting proliferation and inducing contact-growth inhibition and apoptosis. In the current investigation, we determined that loss of Merlin in breast cancer tissues is concordant with the loss of the...


    pub_type: 杂志文章


    authors: Mota MSV,Jackson WP,Bailey SK,Vayalil P,Landar A,Rostas JW 3rd,Mulekar MS,Samant RS,Shevde LA

    更新日期:2018-09-21 00:00:00

  • Glucolimnanthin, a plant glucosinolate, increases the metabolism and DNA binding of benzo[a]pyrene in hamster embryo cell cultures.

    abstract::Glucosinolates are common components of cruciferous vegetables that can be hydrolyzed during food processing to yield isothiocyanates, some of which have been shown to inhibit the induction of mammary tumors in rats by 7,12-dimethyl-benz[a]anthracene. To determine how intact glucosinolates affect the metabolism of ben...


    pub_type: 杂志文章


    authors: Baird WM,Zennie TM,Ferin M,Chae YH,Hatchell J,Cassady JM

    更新日期:1988-04-01 00:00:00

  • Epigenetic therapy using the histone deacetylase inhibitor for increasing therapeutic gain in oral cancer: prevention of radiation-induced oral mucositis and inhibition of chemical-induced oral carcinogenesis.

    abstract::In addition to genetic changes, epigenetic aberrations also play important roles in radiation- and chemical-induced disorders and carcinogenesis. The present study investigated whether epigenetic therapy with a histone deacetylase (HDAC) inhibitor has dual benefits for radiation-induced oral mucositis and chemical-ind...


    pub_type: 杂志文章


    authors: Chung YL,Lee MY,Pui NN

    更新日期:2009-08-01 00:00:00