Spermiogenesis deficiency and germ-cell apoptosis in CREM-mutant mice.


:Spermiogenesis is a complex process by which postmeiotic male germ cells differentiate into mature spermatozoa. This process involves remarkable structural and biochemical changes including nuclear DNA compaction and acrosome formation. Transcription activator CREM (cyclic AMP-responsive element modulator) is highly expressed in postmeiotic cells, and CREM may be responsible for the activation of several haploid germ cell-specific genes involved in the structuring of the spermatozoon. The specific role of CREM in spermiogenesis was addressed using CREM-mutant mice generated by homologous recombination. Analysis of the seminiferous epithelium in mutant male mice reveals postmeiotic arrest at the first step of spermiogenesis. Late spermatids are completely absent, and there is a significant increase in apoptotic germ cells. We show that CREM deficiency results in the lack of postmeiotic cell-specific gene expression. The complete lack of spermatozoa in the mutant mice is reminiscent of cases of human infertility.






Nantel F,Monaco L,Foulkes NS,Masquilier D,LeMeur M,Henriksén K,Dierich A,Parvinen M,Sassone-Corsi P




Has Abstract


1996-03-14 00:00:00














  • The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network.

    abstract::Nuclear exclusion of the PTEN (phosphatase and tensin homologue deleted in chromosome 10) tumour suppressor has been associated with cancer progression. However, the mechanisms leading to this aberrant PTEN localization in human cancers are currently unknown. We have previously reported that ubiquitinylation of PTEN a...


    pub_type: 杂志文章


    authors: Song MS,Salmena L,Carracedo A,Egia A,Lo-Coco F,Teruya-Feldstein J,Pandolfi PP

    更新日期:2008-10-09 00:00:00

  • Evidence that a free-running oscillator drives G1 events in the budding yeast cell cycle.

    abstract::In yeast and somatic cells, mechanisms ensure cell-cycle events are initiated only when preceding events have been completed. In contrast, interruption of specific cell-cycle processes in early embryonic cells of many organisms does not affect the timing of subsequent events, indicating that cell-cycle events are trig...


    pub_type: 杂志文章


    authors: Haase SB,Reed SI

    更新日期:1999-09-23 00:00:00

  • A crystallizing dense magma ocean at the base of the Earth's mantle.

    abstract::The distribution of geochemical species in the Earth's interior is largely controlled by fractional melting and crystallization processes that are intimately linked to the thermal state and evolution of the mantle. The existence of patches of dense partial melt at the base of the Earth's mantle, together with estimate...


    pub_type: 杂志文章


    authors: Labrosse S,Hernlund JW,Coltice N

    更新日期:2007-12-06 00:00:00

  • ATP is a mediator of chemosensory transduction in the central nervous system.

    abstract::Extracellular signalling by the purine nucleotide ATP has long been associated with sensory function. In the periphery, ATP mediates nociception, mechanosensitivity, thermal sensitivity and O2 chemosensitivity. These processes share a common mechanism that involves the release of ATP to excite afferent fibres via acti...


    pub_type: 杂志文章


    authors: Gourine AV,Llaudet E,Dale N,Spyer KM

    更新日期:2005-07-07 00:00:00

  • Water vapour jets inside the plume of gas leaving Enceladus.

    abstract::A plume of water vapour escapes from fissures crossing the south polar region of the Saturnian moon Enceladus. Tidal deformation of a thin surface crust above an internal ocean could result in tensile and compressive stresses that would affect the width of the fissures; therefore, the quantity of water vapour released...


    pub_type: 杂志文章


    authors: Hansen CJ,Esposito LW,Stewart AI,Meinke B,Wallis B,Colwell JE,Hendrix AR,Larsen K,Pryor W,Tian F

    更新日期:2008-11-27 00:00:00

  • Structural basis for transcription elongation by bacterial RNA polymerase.

    abstract::The RNA polymerase elongation complex (EC) is both highly stable and processive, rapidly extending RNA chains for thousands of nucleotides. Understanding the mechanisms of elongation and its regulation requires detailed information about the structural organization of the EC. Here we report the 2.5-A resolution struct...


    pub_type: 杂志文章


    authors: Vassylyev DG,Vassylyeva MN,Perederina A,Tahirov TH,Artsimovitch I

    更新日期:2007-07-12 00:00:00

  • Hidden magnetism and quantum criticality in the heavy fermion superconductor CeRhIn5.

    abstract::With only a few exceptions that are well understood, conventional superconductivity does not coexist with long-range magnetic order (for example, ref. 1). Unconventional superconductivity, on the other hand, develops near a phase boundary separating magnetically ordered and magnetically disordered phases. A maximum in...


    pub_type: 杂志文章


    authors: Park T,Ronning F,Yuan HQ,Salamon MB,Movshovich R,Sarrao JL,Thompson JD

    更新日期:2006-03-02 00:00:00

  • Mice lacking the M3 muscarinic acetylcholine receptor are hypophagic and lean.

    abstract::Members of the muscarinic acetylcholine receptor family (M1-M5) have central roles in the regulation of many fundamental physiological functions. Identifying the specific receptor subtype(s) that mediate the diverse muscarinic actions of acetylcholine is of considerable therapeutic interest, but has proved difficult p...


    pub_type: 杂志文章


    authors: Yamada M,Miyakawa T,Duttaroy A,Yamanaka A,Moriguchi T,Makita R,Ogawa M,Chou CJ,Xia B,Crawley JN,Felder CC,Deng CX,Wess J

    更新日期:2001-03-08 00:00:00

  • A complement-microglial axis drives synapse loss during virus-induced memory impairment.

    abstract::Over 50% of patients who survive neuroinvasive infection with West Nile virus (WNV) exhibit chronic cognitive sequelae. Although thousands of cases of WNV-mediated memory dysfunction accrue annually, the mechanisms responsible for these impairments are unknown. The classical complement cascade, a key component of inna...


    pub_type: 杂志文章


    authors: Vasek MJ,Garber C,Dorsey D,Durrant DM,Bollman B,Soung A,Yu J,Perez-Torres C,Frouin A,Wilton DK,Funk K,DeMasters BK,Jiang X,Bowen JR,Mennerick S,Robinson JK,Garbow JR,Tyler KL,Suthar MS,Schmidt RE,Stevens B,Klein

    更新日期:2016-06-23 00:00:00

  • Association of folding intermediates of glycoproteins with calnexin during protein maturation.

    abstract::Calnexin, an endoplasmic reticulum transmembrane protein, represents a new type of molecular chaperone that selectively associates in a transient fashion with newly synthesized monomeric glycoproteins in HepG2 cells. Calnexin only recognizes glycoproteins when they are incompletely folded. Dissociation of glycoprotein...


    pub_type: 杂志文章


    authors: Ou WJ,Cameron PH,Thomas DY,Bergeron JJ

    更新日期:1993-08-26 00:00:00

  • Detection of metastable electronic states by Penning trap mass spectrometry.

    abstract::State-of-the-art optical clocks1 achieve precisions of 10-18 or better using ensembles of atoms in optical lattices2,3 or individual ions in radio-frequency traps4,5. Promising candidates for use in atomic clocks are highly charged ions6 (HCIs) and nuclear transitions7, which are largely insensitive to external pertur...


    pub_type: 杂志文章


    authors: Schüssler RX,Bekker H,Braß M,Cakir H,Crespo López-Urrutia JR,Door M,Filianin P,Harman Z,Haverkort MW,Huang WJ,Indelicato P,Keitel CH,König CM,Kromer K,Müller M,Novikov YN,Rischka A,Schweiger C,Sturm S,Ulmer S,Elis

    更新日期:2020-05-01 00:00:00

  • Dynamic cortical activity in the human brain reveals motor equivalence.

    abstract::That animals and humans can accomplish the same goal using different effectors and different goals using the same effectors attests to the remarkable flexibility of the central nervous system. This phenomenon has been termed 'motor equivalence', an example being the writing of a name with a pencil held between the toe...


    pub_type: 杂志文章


    authors: Kelso JA,Fuchs A,Lancaster R,Holroyd T,Cheyne D,Weinberg H

    更新日期:1998-04-23 00:00:00

  • Topological domains in mammalian genomes identified by analysis of chromatin interactions.

    abstract::The spatial organization of the genome is intimately linked to its biological function, yet our understanding of higher order genomic structure is coarse, fragmented and incomplete. In the nucleus of eukaryotic cells, interphase chromosomes occupy distinct chromosome territories, and numerous models have been proposed...


    pub_type: 杂志文章


    authors: Dixon JR,Selvaraj S,Yue F,Kim A,Li Y,Shen Y,Hu M,Liu JS,Ren B

    更新日期:2012-04-11 00:00:00

  • A distance to the Large Magellanic Cloud that is precise to one per cent.

    abstract::In the era of precision cosmology, it is essential to determine the Hubble constant empirically with an accuracy of one per cent or better1. At present, the uncertainty on this constant is dominated by the uncertainty in the calibration of the Cepheid period-luminosity relationship2,3 (also known as the Leavitt law). ...


    pub_type: 杂志文章


    authors: Pietrzyński G,Graczyk D,Gallenne A,Gieren W,Thompson IB,Pilecki B,Karczmarek P,Górski M,Suchomska K,Taormina M,Zgirski B,Wielgórski P,Kołaczkowski Z,Konorski P,Villanova S,Nardetto N,Kervella P,Bresolin F,Kudritzki RP

    更新日期:2019-03-01 00:00:00

  • Degeneration in vitro of post-mitotic neurons overexpressing the Alzheimer amyloid protein precursor.

    abstract::A pathological hallmark of Alzheimer's disease is the deposition of amyloid fibrils in the brain. The principal component of amyloid fibrils is beta/A4 amyloid protein, which can be generated by the aberrant processing of a large membrane-bound glycoprotein, the beta/A4 amyloid protein precursor (APP)3. To test whethe...


    pub_type: 杂志文章


    authors: Yoshikawa K,Aizawa T,Hayashi Y

    更新日期:1992-09-03 00:00:00

  • Palaeontological evidence for an Oligocene divergence between Old World monkeys and apes.

    abstract::Apes and Old World monkeys are prominent components of modern African and Asian ecosystems, yet the earliest phases of their evolutionary history have remained largely undocumented. The absence of crown catarrhine fossils older than ∼20 million years (Myr) has stood in stark contrast to molecular divergence estimates ...


    pub_type: 历史文章,杂志文章


    authors: Stevens NJ,Seiffert ER,O'Connor PM,Roberts EM,Schmitz MD,Krause C,Gorscak E,Ngasala S,Hieronymus TL,Temu J

    更新日期:2013-05-30 00:00:00

  • Hippocampal neurogenesis confers stress resilience by inhibiting the ventral dentate gyrus.

    abstract::Adult neurogenesis in the dentate gyrus of the hippocampus is highly regulated by environmental influences, and functionally implicated in behavioural responses to stress and antidepressants1-4. However, how adult-born neurons regulate dentate gyrus information processing to protect from stress-induced anxiety-like be...


    pub_type: 杂志文章


    authors: Anacker C,Luna VM,Stevens GS,Millette A,Shores R,Jimenez JC,Chen B,Hen R

    更新日期:2018-07-01 00:00:00

  • Contour from motion processing occurs in primary visual cortex.

    abstract::Relative motion is one of the most salient cues for segmentation of a visual scene into separate objects. This is illustrated by the vivid contours that are perceived when random dot patterns move in different directions. Once motion is halted in such displays the segmentation contours disappear. This makes random dot...


    pub_type: 杂志文章


    authors: Lamme VA,van Dijk BW,Spekreijse H

    更新日期:1993-06-10 00:00:00

  • Sodium channel mutation leading to saxitoxin resistance in clams increases risk of PSP.

    abstract::Bivalve molluscs, the primary vectors of paralytic shellfish poisoning (PSP) in humans, show marked inter-species variation in their capacity to accumulate PSP toxins (PSTs) which has a neural basis. PSTs cause human fatalities by blocking sodium conductance in nerve fibres. Here we identify a molecular basis for inte...


    pub_type: 杂志文章


    authors: Bricelj VM,Connell L,Konoki K,Macquarrie SP,Scheuer T,Catterall WA,Trainer VL

    更新日期:2005-04-07 00:00:00

  • Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis.

    abstract::The protease responsible for the cleavage of poly(ADP-ribose) polymerase and necessary for apoptosis has been purified and characterized. This enzyme, named apopain, is composed of two subunits of relative molecular mass (M(r)) 17K and 12K that are derived from a common proenzyme identified as CPP32. This proenzyme is...


    pub_type: 杂志文章


    authors: Nicholson DW,Ali A,Thornberry NA,Vaillancourt JP,Ding CK,Gallant M,Gareau Y,Griffin PR,Labelle M,Lazebnik YA

    更新日期:1995-07-06 00:00:00

  • Control of TH17 cells occurs in the small intestine.

    abstract::Interleukin (IL)-17-producing T helper cells (T(H)17) are a recently identified CD4(+) T cell subset distinct from T helper type 1 (T(H)1) and T helper type 2 (T(H)2) cells. T(H)17 cells can drive antigen-specific autoimmune diseases and are considered the main population of pathogenic T cells driving experimental aut...


    pub_type: 杂志文章


    authors: Esplugues E,Huber S,Gagliani N,Hauser AE,Town T,Wan YY,O'Connor W Jr,Rongvaux A,Van Rooijen N,Haberman AM,Iwakura Y,Kuchroo VK,Kolls JK,Bluestone JA,Herold KC,Flavell RA

    更新日期:2011-07-17 00:00:00

  • Reversing histone methylation.

    abstract::Histones package DNA, and post-translational modifications of histones can regulate access to DNA. Until recently, histone methylation-unlike all other histone modifications-was considered a permanent mark. The discovery of enzymes that reverse the methylation of lysines and arginines challenges our current thinking o...


    pub_type: 杂志文章,评审


    authors: Bannister AJ,Kouzarides T

    更新日期:2005-08-25 00:00:00

  • Germ-line transmission and expression of a human-derived yeast artificial chromosome.

    abstract::Introduction of DNA fragments, hundreds of kilobases in size, into mouse embryonic stem (ES) cells would greatly advance the ability to manipulate the mouse genome. Mice generated from such modified cells would permit investigation of the function and expression of very large or crudely mapped genes. Large DNA molecul...


    pub_type: 杂志文章


    authors: Jakobovits A,Moore AL,Green LL,Vergara GJ,Maynard-Currie CE,Austin HA,Klapholz S

    更新日期:1993-03-18 00:00:00

  • High-throughput discovery of novel developmental phenotypes.

    abstract::Approximately one-third of all mammalian genes are essential for life. Phenotypes resulting from knockouts of these genes in mice have provided tremendous insight into gene function and congenital disorders. As part of the International Mouse Phenotyping Consortium effort to generate and phenotypically characterize 5,...


    pub_type: 杂志文章


    authors: Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs

    更新日期:2016-09-22 00:00:00

  • A stomatin-domain protein essential for touch sensation in the mouse.

    abstract::Touch and mechanical pain are first detected at our largest sensory surface, the skin. The cell bodies of sensory neurons that detect such stimuli are located in the dorsal root ganglia, and subtypes of these neurons are specialized to detect specific modalities of mechanical stimuli. Molecules have been identified th...


    pub_type: 杂志文章


    authors: Wetzel C,Hu J,Riethmacher D,Benckendorff A,Harder L,Eilers A,Moshourab R,Kozlenkov A,Labuz D,Caspani O,Erdmann B,Machelska H,Heppenstall PA,Lewin GR

    更新日期:2007-01-11 00:00:00

  • Episodic fresh surface waters in the Eocene Arctic Ocean.

    abstract::It has been suggested, on the basis of modern hydrology and fully coupled palaeoclimate simulations, that the warm greenhouse conditions that characterized the early Palaeogene period (55-45 Myr ago) probably induced an intensified hydrological cycle with precipitation exceeding evaporation at high latitudes. Little f...


    pub_type: 历史文章,杂志文章


    authors: Brinkhuis H,Schouten S,Collinson ME,Sluijs A,Sinninghe Damsté JS,Dickens GR,Huber M,Cronin TM,Onodera J,Takahashi K,Bujak JP,Stein R,van der Burgh J,Eldrett JS,Harding IC,Lotter AF,Sangiorgi F,van Konijnenburg-van Citte

    更新日期:2006-06-01 00:00:00

  • T-helper-1-cell cytokines drive cancer into senescence.

    abstract::Cancer control by adaptive immunity involves a number of defined death and clearance mechanisms. However, efficient inhibition of exponential cancer growth by T cells and interferon-γ (IFN-γ) requires additional undefined mechanisms that arrest cancer cell proliferation. Here we show that the combined action of the T-...


    pub_type: 杂志文章


    authors: Braumüller H,Wieder T,Brenner E,Aßmann S,Hahn M,Alkhaled M,Schilbach K,Essmann F,Kneilling M,Griessinger C,Ranta F,Ullrich S,Mocikat R,Braungart K,Mehra T,Fehrenbacher B,Berdel J,Niessner H,Meier F,van den Broek M,

    更新日期:2013-02-21 00:00:00

  • Support for a synaptic chain model of neuronal sequence generation.

    abstract::In songbirds, the remarkable temporal precision of song is generated by a sparse sequence of bursts in the premotor nucleus HVC. To distinguish between two possible classes of models of neural sequence generation, we carried out intracellular recordings of HVC neurons in singing zebra finches (Taeniopygia guttata). We...


    pub_type: 杂志文章


    authors: Long MA,Jin DZ,Fee MS

    更新日期:2010-11-18 00:00:00

  • A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene.

    abstract::The metabolic basis of the autosomal recessive disease cystic fibrosis (CF) remains unidentified. Elevated levels of a serum protein in CF homozygotes and obligate heterozygotes have been described. As heterozygotes are clinically unaffected, any consistently observed abnormality in these individuals is a likely point...


    pub_type: 杂志文章


    authors: Dorin JR,Novak M,Hill RE,Brock DJ,Secher DS,van Heyningen V

    更新日期:1987-04-09 00:00:00

  • A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.

    abstract::The cellular oncogene c-Ki-ras is amplified 30- to 60-fold in cells of the mouse adrenocortical tumour Y1. The amplified oncogene is located in double minute chromosomes and in a homogeneously staining chromosomal region, common karyotypical anomalies of tumour cells. The amounts of c-Ki-ras specific mRNA and of the p...


    pub_type: 杂志文章


    authors: Schwab M,Alitalo K,Varmus HE,Bishop JM,George D

    更新日期:1983-06-09 00:00:00