Abstract：
:Three monoclonal antibodies (MAbs) against p53 protein (PAb 24o, DO-I and PAb1801) were used to define the immunophenotype of 13 melanoma cell lines. Immunoreactions could be detected in 12 out of 13 cell lines by using the indirect immunofluorescence technique. In 7 of these the majority of cells displayed cytoplasmic staining whereas positive nuclei were detected in only a few cells. Two cell lines had predominantly nuclear reactivity, while the remaining 3 cell lines showed signals in both locations. Despite identical nuclear staining patterns, the 3 MAbs produced qualitatively distinct cytoplasmic immunoreactions. PAb240 and DO-1, which showed similar staining frequencies, appeared more sensitive in the detection of p53 protein than did PAb1801. Immunoprecipitations of lysates from each of the cell lines, with MAbs DO-1 and 1801 (which bind to both wild-type and mutant p53 species) detected 53-kDa proteins, whereas PAb240 (which recognizes the mutant conformation of the protein in this type of assay) detected 53-kDa proteins in only 4 cell lines. Nucleotide sequencing of exons 5 to 9 of TP53 in these latter cell lines showed that each has homozygous point mutations in the locus, whereas in the others no TP53 alterations were found. Three of the 4 mutations were C-to-T transversions, alterations possibly caused by damage from UV-light. Our findings indicate that immunostaining with p53 antibodies, although common in malignant melanoma, results from the presence of mutant p53 protein in about 30% of the cases tested. Neither immunostaining with PAb240 nor the patterns of intracellular distributions of the signals are sufficient to detect TP53 mutations.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Weiss J,Schwechheimer K,Cavenee WK,Herlyn M,Arden KCdoi
10.1002/ijc.2910540427subject
Has Abstractpub_date
1993-06-19 00:00:00pages
693-9issue
4eissn
0020-7136issn
1097-0215journal_volume
54pub_type
杂志文章abstract：:The incidence of testicular cancer was examined in the Nordic and Baltic countries, Poland and Germany by collaboration among 10 cancer registries. Population-based registers were used to analyze a total of 34,309 cases, diagnosed from the start of registration (varying from 1943 in Denmark to 1980 in Latvia and Lithu...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910590108
更新日期：1994-10-01 00:00:00
abstract：:Folate receptor alpha (FRA) is a GPI-anchored glycoprotein and encoded by the FOLR1 gene. High expression of FRA is observed in specific malignant tumors of epithelial origin, including ovarian cancer, but exhibits very limited normal tissue expression, making it as an attractive target for the ovarian cancer therapy....
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29937
更新日期：2016-04-15 00:00:00
abstract：:The growth of 7/9 Burkitt's lymphoma (BL) cell lines and 5/6 human melanoma cell lines was inhibited by certain human fibroblast-like cell lines, especially human myofibroblast cell lines. In contrast to BL lines, 3/5 lymphoblastoid cell lines were less susceptible and 3/4 human leukaemic lines were resistant. The eff...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910350410
更新日期：1985-04-15 00:00:00
abstract：:The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell l...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20000701)87:1<37::aid-ijc6>3.0.c
更新日期：2000-07-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity, representing 90% of all oral carcinomas and accounting for 3-5% of all malignancies. The WWOX gene (WW-domain containing oxidoreductase) is a candidate tumor suppressor gene located at 16q23.3-24.1, spanning the second most c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21446
更新日期：2006-03-01 00:00:00
abstract：:Turkey poults were inoculated with lymphoproliferative disease virus (LPDV) of turkey, and the organotropism of the inoculated virus was determined from the kinetics of virus expression and tumor formation. Molecular hybridization experiments, conducted to determine the level of viral RNA expression in the various org...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910290518
更新日期：1982-05-15 00:00:00
abstract：:Cells of the melanocytic lineage have been shown to be capable of producing laminin and to lose this property during transformation. In the present study, we have evaluated immunohistochemically whether these changes are paralleled by an altered expression of the alpha 6/beta 1 laminin receptor. Results of this analys...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910490203
更新日期：1991-09-09 00:00:00
abstract：:There is good evidence for an association between Epstein-Barr virus (EBV) and Hodgkin's disease (HD). In approximately one-third of cases, the EBV genome is detectable in Reed-Sternberg (RS) cells and there is expression of the viral nuclear antigen EBNA-1 and the latent membrane protein LMP-1. Expression of LMP-2 ha...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19970807)72:4<614::aid-ijc
更新日期：1997-08-07 00:00:00
abstract：:Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Allelic losses on the chromosome arms 1p and 19q have been associated with favorable response to chemotherapy and good prognosis in anaplastic oligodendroglioma patients, but the molecular mechanisms responsible for this relationship are as yet unknown. The DNA repair enzyme O(6)-methylguanine DNA methyltransferase (M...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20575
更新日期：2005-01-20 00:00:00
abstract：:Malignant mesothelioma (MM) is an aggressive tumor associated with environmental or occupational exposure to asbestos fibers. Erionite is a fibrous zeolite, morphologically similar to asbestos and it is assumed to be even more carcinogenic. Onset and progression of MM has been suggested as the result of the cooperatio...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22687
更新日期：2007-07-01 00:00:00
abstract：:Suppressor of cytokine signaling 3 (SOCS3), as a key regulator of cytokine signaling, has the potential to modulate numerous cellular processes. Its involvement in inflammatory disease is well established, and there is increasing evidence for a role in breast cancer as a regulator of signal transducers and activators ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.24172
更新日期：2009-04-15 00:00:00
abstract：:Nuclear factor-κB (NF-κB) is constitutively activated in many cancers, including oral squamous cell carcinoma (OSCC), and is involved in the invasive characteristics of OSCC, such as growth, antiapoptotic activity and protease production. However, the cellular mechanism underlying NF-κB's promotion of bone invasion by...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.27435
更新日期：2012-09-01 00:00:00
abstract：:Single-cell suspensions prepared from five human melanomas, grown serially as xenografts in athymic nude mice, were exposed in vitro to increasing concentrations of DTIC (Dacarbazine), CCNU (Lomustine), procarbazine, vinblastine, and the cancerostatic lectins abrin and ricin. The in vitro chemosensitivity of the cells...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910260604
更新日期：1980-12-15 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis in tumors. Basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) have both been shown to interact with VEGF. The involvement of VEGF, bFGF, and EGF in melanoma angiogenesis was investigated here. Four human melanoma cell lin...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980610)76:6<836::aid-ijc
更新日期：1998-06-10 00:00:00
abstract：:In STU mice bearing metastasizing SV40-transformed 51A-232B-M tumors, an immune response against a cellular 60kDa protein (p60) developed in about 50% of the tumor-bearing animals, in addition to the response against SV40 large T-antigen and cellular protein p53. The anti-p60 auto-immune response could be observed as ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910420620
更新日期：1988-12-15 00:00:00
abstract：:The metastatic spread of tumor cells is the most lethal aspect of cancer and can occur via various routes, including the lymphatic vasculature. Studies of tumor models in animals and clinicopathological data have indicated that growth of lymphatic vessels (lymphangiogenesis) in the vicinity of solid tumors may contrib...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.21899
更新日期：2006-10-15 00:00:00
abstract：:Physical activity might reduce the risk for lung cancer by various mechanisms, but the evidence is inconclusive. We therefore examined the relationship between physical activity and risk for lung cancer in a large population-based Danish cohort with detailed information about number of hours per week spent on specific...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21085
更新日期：2005-09-01 00:00:00
abstract：:Expression of the candidate metastasis-suppressor gene nm23-H1 has been shown to correlate inversely with metastatic potential in some human tumors, but not in all. Until now, few studies have been carried out on the activity of the homologous nm23-H2 gene in human cancer. No nm23 transcription studies exist for human...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910550306
更新日期：1993-09-30 00:00:00
abstract：:Epidermal growth factor receptor (EGFR) plays an important role in essential cellular processes such as proliferation, survival and migration. Aberrant activation of EGFR is frequently found in human cancers of various origins and has been implicated in cancer pathogenesis. The therapeutic antibody cetuximab (Erbitux)...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28585
更新日期：2014-06-01 00:00:00
abstract：:Neoplasms constituted an enormous burden and contributed to an estimated 8.2 million deaths in 2012 worldwide. FOXO1 (forkhead box O1), a member of the forkhead box (FOX) family, is a transcriptional factor involved in diverse cellular functions. Herein, we concentrate on recent studies of the antineoplastic roles of ...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.31338
更新日期：2018-10-01 00:00:00
abstract：:The mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) encodes a protein that plays a critical role in tumor suppression, in part by modulating bioavailability of a potent mitogen, insulin-like growth factor-2 (IGF2). We tested the hypothesis that the common nonsynonymous genetic variants in M6P/IGF...
journal_title：International journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1002/ijc.24623
更新日期：2009-12-01 00:00:00
abstract：:The cytokines that regulate angiogenesis in normal and malignant prostate tissue are not well studied. Using an RT-PCR-based screen, we observed that cultured, low-passage normal human prostate epithelial cells (PrECs) express a variety of cytokines which have been shown to have angiogenic and/or endothelial cell-acti...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19990315)80:6<868::aid-ijc
更新日期：1999-03-15 00:00:00
abstract：:Exfoliated nasopharyngeal (NP) cells from 62 normal Cantonese Chinese having IgA/VCA antibodies for more than a year and from 39 similar persons without IgA/VCA antibodies, were tested for the presence of EBV/DNA sequences by spot followed by blot hybridization tests, using the cloned internal repeat of B95-8 viral DN...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910320504
更新日期：1983-11-15 00:00:00
abstract：:The mutated in colorectal cancer (MCC) is a multifunctional gene showing loss of expression in colorectal and liver cancers. MCC mutations can drive colon carcinogenesis in the mouse and in vitro experiments suggest that loss of MCC function promotes cancer through several important cellular pathways. In particular, t...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28967
更新日期：2015-01-01 00:00:00
abstract：:Leukocyte-transforming agents were isolated in baboon leukocytes inoculated with oral excretions from immunosuppressed chimpanzees. The transformed lymphoblasts had B cell surface markers and harbored herpes-type virus particles; 5-10% of the cells contained cytoplasmic antigens reactive with Epstein-Barr virus (EBV)-...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910200318
更新日期：1977-09-15 00:00:00
abstract：:The amplification of the q21 band of chromosome 8 (8q21) occurs in a large percentage of breast cancers. WWP1, an HECT domain-containing ubiquitin E3 ligase located in the 8q21 region, negatively regulates the TGF-beta tumor suppressor pathway. To characterize the role of WWP1 in breast cancer, we analyzed WWP1 gene d...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22653
更新日期：2007-07-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) reactive with the ganglioside fucosyl GM1 (Fuc-GM1), an antigen associated with small-cell carcinoma of the lung (SCLC), were tested for their ability to mediate tumor-cell killing in vitro in conjunction with humoral and cellular effectors and to inhibit tumor engraftment in nude mice in ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910490619
更新日期：1991-12-02 00:00:00
abstract：:Inactivation of the tumor-suppressor gene p16 (MTS1/ CDKN2/INK4a) has been described in various human malignancies. Although p16 deletion has been found in various ovarian tumor cell lines, p16 inactivation by homozygous deletion or mutation has been reported only sporadically in primary ovarian carcinomas. In a compr...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980220)79:1<61::aid-ijc1
更新日期：1998-02-20 00:00:00
abstract：:The interactions of MCF-7 tumor cells with human vascular endothelial cells (EC) and subendothelial extracellular matrices (ECM) were morphologically observed by electron microscopy and quantitatively evaluated by labelling tumor cells with 111Indium-oxine. MCF-7 tumor cells adhered more rapidly to ECM than to the api...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910400416
更新日期：1987-10-15 00:00:00
