Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.

abstract：:Canonical Wnt signaling plays an important role in development and disease, regulating transcription of target genes and stabilizing many proteins phosphorylated by glycogen synthase kinase 3 (GSK3). We observed that the MiT family of transcription factors, which includes the melanoma oncogene MITF (micropthalmia-asso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ploper D,Taelman VF,Robert L,Perez BS,Titz B,Chen HW,Graeber TG,von Euw E,Ribas A,De Robertis EM

更新日期：2015-02-03 00:00:00

abstract：:A high-resolution compositional map of glass-forming ability (GFA) in the Ni-Cr-Nb-P-B system is experimentally determined along various compositional planes. GFA is shown to be a piecewise continuous function formed by intersecting compositional subsurfaces, each associated with a nucleation pathway for a specific cr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Na JH,Demetriou MD,Floyd M,Hoff A,Garrett GR,Johnson WL

更新日期：2014-06-24 00:00:00

abstract：:Accumulation of misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response (UPR), an intracellular signaling pathway that adjusts the protein folding capacity of the ER according to need. If homeostasis in the ER protein folding environment cannot be reestablished, cells commit to apop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Korennykh AV,Behrman SL,Walter P

更新日期：2010-09-14 00:00:00

abstract：:Gene regulation by imposed localization was studied by using designed zinc finger proteins that bind 18-bp DNA sequences in the 5' untranslated regions of the protooncogenes erbB-2 and erbB-3. Transcription factors were generated by fusion of the DNA-binding proteins to repression or activation domains. When introduce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beerli RR,Dreier B,Barbas CF 3rd

更新日期：2000-02-15 00:00:00

abstract：:A construct, MRE-beta Geo, with five metal response elements fused to a selectable reporter gene was transfected into BHK cells and a stable clone that could be induced up to 100-fold by zinc, cadmium, bismuth, silver, cobalt, copper, mercury, or nickle was isolated. Some, and perhaps all, of these metals induce MRE-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palmiter RD

更新日期：1994-02-15 00:00:00

abstract：:Photoactivation of the retinal photoreceptor rhodopsin proceeds through a cascade of intermediates, resulting in protein-protein interactions catalyzing the activation of the G-protein transducin (Gt). Using stabilization and photoregeneration of the receptor's signaling state and Gt activation assays, we provide evid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kisselev OG,Meyer CK,Heck M,Ernst OP,Hofmann KP

更新日期：1999-04-27 00:00:00

abstract：:In Trimenia moorei, an extant member of the ancient angiosperm clade Austrobaileyales, we found a remarkable pattern of female gametophyte (egg-producing structure) development that strikingly resembles that of pollen tubes and their intrasexual competition within the maternal pollen tube transmitting tissues of most ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachelier JB,Friedman WE

更新日期：2011-07-26 00:00:00

abstract：:Cognitive flexibility depends on a fast neural learning mechanism for enhancing momentary relevant over irrelevant information. A possible neural mechanism realizing this enhancement uses fast spiking interneurons (FSIs) in the striatum to train striatal projection neurons to gate relevant and suppress distracting cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banaie Boroujeni K,Oemisch M,Hassani SA,Womelsdorf T

更新日期：2020-07-28 00:00:00

abstract：:Growing knowledge of the key molecular components involved in biological processes such as endocytosis, exocytosis, and motility has enabled direct testing of proposed mechanistic models by reconstitution. However, current techniques for building increasingly complex cellular structures and functions from purified com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richmond DL,Schmid EM,Martens S,Stachowiak JC,Liska N,Fletcher DA

更新日期：2011-06-07 00:00:00

abstract：:Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis. Two pyrrole-imidazole polyamides were designed to bind DNA sequences immediately adja...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickinson LA,Gulizia RJ,Trauger JW,Baird EE,Mosier DE,Gottesfeld JM,Dervan PB

更新日期：1998-10-27 00:00:00

abstract：:Surgical removal of one or both atrial appendages was employed in rats to reduce the intrinsic stores of atriopeptin (AP). In conscious rats (with intact baroreceptor reflexes), bilateral or unilateral atrial appendectomy suppressed the diuresis and natriuresis produced by acute volume expansion. Surprisingly, volume ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakata M,Greenwald JE,Needleman P

更新日期：1988-05-01 00:00:00

abstract：:Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van der Werf S,Wychowski C,Bruneau P,Blondel B,Crainic R,Horodniceanu F,Girard M

更新日期：1983-08-01 00:00:00

abstract：:DNA polymerases from Xenopus laevis oocytes, unfertilized eggs, and kidney cells grown in culture have been characterized. The same three major DNA polymerase activities are present in all cell types examined. We attempt to relate the characteristics of amphibian enzymes to those of mammalian polymerases. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tatò F,Gandini DA,Tocchini-Valentini GP

更新日期：1974-09-01 00:00:00

abstract：:Recoverin, a recently discovered member of the EF-hand superfamily of Ca(2+)-binding proteins, serves as a Ca2+ sensor in vision. The amino terminus of the protein from retinal rod cells contains a covalently attached myristoyl or related N-acyl group. We report here studies of unmyristoylated and myristoylated recomb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zozulya S,Stryer L

更新日期：1992-12-01 00:00:00

abstract：:Relatively little is known about the biochemical mechanisms through which the Epstein-Barr virus latent infection integral membrane protein 1 (LMP1) transmembrane domains cause constitutive LMP1 aggregation and continuous cytoplasmic C terminus-mediated signal transduction. We now evaluate the role of the three consec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yasui T,Luftig M,Soni V,Kieff E

更新日期：2004-01-06 00:00:00

abstract：:In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doelling JH,Gaudino RJ,Pikaard CS

更新日期：1993-08-15 00:00:00

abstract：:The evolution of sensory systems has let mammals develop complicated tactile end organs to enable sophisticated sensory tasks, including social interaction, environmental exploration, and tactile discrimination. The Merkel disc, a main type of tactile end organ consisting of Merkel cells (MCs) and Aβ-afferent endings,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang W,Kanda H,Ikeda R,Ling J,DeBerry JJ,Gu JG

更新日期：2016-09-13 00:00:00

abstract：:Protective immunity against human schistosome infection develops slowly, for reasons that are not yet fully understood. For many decades, researchers have attempted to infer properties of the immune response from epidemiological studies, with mathematical models frequently being used to bridge the gap between immunolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell KM,Mutapi F,Savill NJ,Woolhouse ME

更新日期：2012-08-14 00:00:00

abstract：:Previous attempts to express functional DNA cytosine methyltransferase (EC 2.1.1.37) in cells transfected with the available Dnmt cDNAs have met with little or no success. We show that the published Dnmt sequence encodes an amino terminal-truncated protein that is tolerated only at very low levels when stably expresse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tucker KL,Talbot D,Lee MA,Leonhardt H,Jaenisch R

更新日期：1996-11-12 00:00:00

abstract：:Animal epithelial tissue becomes reproducibly colonized by specific environmental bacteria. The bacteria (microbiota) perform critical functions for the host's tissue development, immune system development, and nutrition; yet the processes by which bacterial diversity in the environment is selected to assemble the cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brooks JF 2nd,Gyllborg MC,Cronin DC,Quillin SJ,Mallama CA,Foxall R,Whistler C,Goodman AL,Mandel MJ

更新日期：2014-12-02 00:00:00

abstract：:Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turcotte R,Liang Y,Tanimoto M,Zhang Q,Li Z,Koyama M,Betzig E,Ji N

更新日期：2019-05-07 00:00:00

abstract：:Eleocharis vivipara Link, a freshwater amphibious leafless plant belonging to the Cyperaceae can grow in both terrestrial and submersed aquatic conditions. Two forms of E. vivipara obtained from these contrasting environments were examined for the characteristics associated with C(4) and C(3) photosynthesis. In the te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ueno O,Samejima M,Muto S,Miyachi S

更新日期：1988-09-01 00:00:00

abstract：:Hearing is one of the last sensory modalities to be subjected to genetic analysis in Drosophila melanogaster. We describe a behavioral assay for auditory function involving courtship among groups of males triggered by the pulse component of the courtship song. In a mutagenesis screen for mutations that disrupt the aud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eberl DF,Duyk GM,Perrimon N

更新日期：1997-12-23 00:00:00

abstract：:Aminoacyl-tRNA synthetases are multidomain proteins responsible for the attachment of specific amino acids to their tRNA substrates. Prolyl-tRNA synthetases (ProRSs) are notable due to their particularly diverse architectures through evolution. For example, Saccharomyces cerevisiae ProRS possesses an N-terminal extens...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： SternJohn J,Hati S,Siliciano PG,Musier-Forsyth K

更新日期：2007-02-13 00:00:00

abstract：:In animals, fatty acid desaturases catalyze key reactions in the synthesis of arachidonic acid and other polyunsaturated fatty acids. A search of the Caenorhabditis elegans DNA databases, using the sequences of Arabidopsis genes, identified several putative desaturases. Here we describe the characterization of the fir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spychalla JP,Kinney AJ,Browse J

更新日期：1997-02-18 00:00:00

abstract：:Tolerance and resistance provide hosts with two distinct defense strategies against parasitism. In resistance the hosts "fight" the parasite directly, whereas in tolerance the hosts fight the disease by ameliorating the damage that infection causes. There is increasing recognition that the two mechanisms may exhibit v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Best A,White A,Boots M

更新日期：2008-12-30 00:00:00

abstract：:The labeled inorganic phosphate formed by enzymatic hydrolysis of [gamma-18O]ATP in normal water was derivatized to trimethyl phosphate and analyzed for the proportions of [18O3]Pi, [18O2]Pi, [18O1]Pi, and [18O0]Pi. The proportions observed were correlated with the kinetics of intermediate exchange by using a kinetic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Midelfort CF

更新日期：1981-04-01 00:00:00

abstract：:The output of the cerebellar cortex is controlled by two main inputs, (i.e., the climbing fiber and mossy fiber-parallel fiber pathway) and activations of these inputs elicit characteristic effects in its Purkinje cells: that is, the so-called complex spikes and simple spikes. Target neurons of the Purkinje cells in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoebeek FE,Witter L,Ruigrok TJ,De Zeeuw CI

更新日期：2010-05-04 00:00:00

abstract：:Members of the voltage-gated-like ion channel superfamily have a conserved pore structure. Transmembrane helices that line the pore (M2 or S6) are thought to gate it at the cytoplasmic end by bending at a hinge glycine residue. Proline residues favor bending of alpha-helices, and substitution of proline for this glyci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Y,Scheuer T,Catterall WA

更新日期：2004-12-21 00:00:00

abstract：:Cysteine-bound hemes are key components of many enzymes and biological sensors. Protonation (deprotonation) of the Cys ligand often accompanies redox transformations of these centers. To characterize these phenomena, we have engineered a series of Thr78Cys/Lys79Gly/Met80X mutants of yeast cytochrome c (cyt c) in which...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhong F,Lisi GP,Collins DP,Dawson JH,Pletneva EV

更新日期：2014-01-21 00:00:00