Abstract：
:We undertook a study to determine the role and regulation of expression of urokinase-type plasminogen activator in squamous-cell carcinoma of the oral cavity. The contribution of urokinase to the invasive process was clearly demonstrated in experiments in which in vitro invasion by a cultured squamous-cell carcinoma cell line was substantially reduced by a monoclonal antibody directed at the catalytic site of urokinase. The conditioned medium from 2 invasive cell lines contained high levels of urokinase. Examination of resected tumors for urokinase revealed (a) localization of the antigen to the tumor cells and (b) higher levels of the plasminogen activator in tumor tissue than in adjacent non-malignant tissue. These results suggested that elevated expression of urokinase is a common feature of this malignancy. The mRNA half-lives of cell lines expressing high and low levels of urokinase were similar, indicating that elevated levels of mRNA for the plasminogen activator were not a consequence of a more stable transcript. No evidence of u-PA gene amplification was obtained by Southern blotting of DNA derived from the cell lines expressing high urokinase levels. Transfection of squamous-cell carcinoma cells, which express high levels of urokinase, with a construct bearing the chloramphenicol acetyl transferase gene driven by the full-length (2345 bp) urokinase promoter indicated activation of the urokinase promoter in trans. In conclusion, our results suggest that transcriptional activation of the urokinase gene, in squamous-cell carcinomas of the oral cavity, leads to elevated levels of urokinase mRNA/protein which, in turn, can promote the invasive phenotype.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Clayman G,Wang SW,Nicolson GL,el-Naggar A,Mazar A,Henkin J,Blasi F,Goepfert H,Boyd DDdoi
10.1002/ijc.2910540113subject
Has Abstractpub_date
1993-04-22 00:00:00pages
73-80issue
1eissn
0020-7136issn
1097-0215journal_volume
54pub_type
杂志文章abstract：:The specific aim of three-dimensional conformal radiotherapy is to deliver adequate therapeutic radiation dose to the target volume while concomitantly keeping the dose to surrounding and intervening normal tissues to a minimum. The objective of this study is to examine dose distributions produced by various radiother...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20010220)96:1<55::aid-ijc6>3.0.c
更新日期：2001-02-20 00:00:00
abstract：:Human blood monocytes are very potent to take up antigens. Like macrophages in tissue, they efficiently degrade exogenous protein and are less efficient than dendritic cells (DCs) at cross-presenting antigens to CD8+ T cells. Although it is generally accepted that DCs take up tissue antigens and then migrate to lymph ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31037
更新日期：2018-01-01 00:00:00
abstract：:Twenty-seven human colorectal adenocarcinomas were implanted s.c. into nude mice. A comparative study of chromosomes and DNA between fresh tumor (FT) and xenografted tumor cells (XT) could be performed in 9 cases. Losses or deletions were consistently found in FTs as well as in XTs, particularly on chromosomes 17 and/...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910440521
更新日期：1989-11-15 00:00:00
abstract：:The purpose was to evaluate alternative cytological screening methods in population-based screening for cervical cancer up to cancer incidence and mortality outcome. Automation-assisted screening was compared to conventional cytological screening in a randomized design. The study was based on follow-up of 503,391 wome...
journal_title：International journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1002/ijc.25677
更新日期：2011-03-01 00:00:00
abstract：:To generate non-small cell lung cancer (NSCLC)-reactive lymphocytes, we transfected an HLA-A2-expressing human NSCLC line (1355) with the cDNA encoding the lymphocyte co-stimulatory molecule CD80. Following selection in G418, 1355.7 demonstrated stable cell-surface expression of CD80. Allogeneic mixed lymphocyte tumor...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19981209)78:6<685::aid-ijc
更新日期：1998-12-09 00:00:00
abstract：:Pancreatic ductal adenocarcinoma is among the top 10 causes of death from cancer in industrialized countries. In comparison with other gastrointestinal malignancies, pancreatic cancer is one of the tumors most resistant to chemotherapy. An important mechanism of tumor multidrug resistance is increased drug efflux medi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20831
更新日期：2005-06-20 00:00:00
abstract：:Paclitaxel has antiangiogenic properties, but the mechanisms for the enhanced sensitivity of endothelial cells (ECs) to this drug are not established. The aims of our study were to compare the distribution of paclitaxel into ECs with other cell types, to assess the effects of low doses of paclitaxel on Cox-2 expressio...
journal_title：International journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1002/ijc.20595
更新日期：2005-01-20 00:00:00
abstract：:We have addressed the role of bacterial co-infection in viral oncogenesis using as model Epstein-Barr virus (EBV), a human herpesvirus that causes lymphoid malignancies and epithelial cancers. Infection of EBV carrying epithelial cells with the common oral pathogenic Gram-negative bacterium Aggregatibacter actinomycet...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31652
更新日期：2019-01-01 00:00:00
abstract：:Antioxidants, primarily from fruits and vegetables, have been hypothesized to protect against non-Hodgkin lymphoma (NHL). The oxygen radical absorbance capacity (ORAC) assay, which measures total antioxidant capacity of individual foods and accounts for synergism, can be estimated using a food-frequency questionnaire ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.26491
更新日期：2012-09-01 00:00:00
abstract：:The large geographic variations in the incidence of gastric cancer (GC) are likely due to differential environmental exposures, in particular to Helicobacter pylori (H. pylori) infection. We aimed to investigate the impact of H. pylori on the epigenome in normal gastric mucosa and methylation changes associated with c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31381
更新日期：2018-08-01 00:00:00
abstract：:A population-based case-control study was conducted to evaluate risk of developing multiple myeloma (MM) associated with personal history of autoimmune diseases and occurrence of autoimmune and selected hematologic disorders in first-degree relatives. Data were obtained for all (n = 8,406) MM cases diagnosed in Sweden...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21745
更新日期：2006-06-15 00:00:00
abstract：:We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer and metastatic cancer respectively. We examined the expression of matrix-degrading enzymes and their inhibitors in these cell lines. Both cell lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9,...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19970106)70:1<120::aid-ijc
更新日期：1997-01-06 00:00:00
abstract：:Activation-induced cytidine deaminase (AID) plays a role as a genome mutator in activated B cells, and inappropriate expression of AID has been implicated in the immunopathological phenotype of human B-cell malignancies. Notably, we found that the transgenic mice overexpressing AID developed lung adenocarcinoma and he...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22292
更新日期：2007-02-01 00:00:00
abstract：:The effect of all-trans-retinoic acid (RA) was examined on (1) transformation induced in C127 cells by transfection with plasmid pdBPV-1 (142-6), which contains DNA of bovine papillomavirus (BPV), (2) the capacity of transformed BPV DNA-containing clones to form colonies with transformed properties (e.g., piling up in...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910420118
更新日期：1988-07-15 00:00:00
abstract：:Loss of heterozygosity (LOH) on 10q is associated with late-stage events in urothelial neoplastic progression. The tumor suppressor gene PTEN, which is mutated or homozygously deleted in numerous cancers, maps to a region of 10q within the reported region of minimal loss in bladder tumors. In two recent studies altera...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20001115)88:4<620::aid-ijc16>3.0
更新日期：2000-11-15 00:00:00
abstract：:We examined the effects of acivicin, a specific inhibitor of the ectoenzyme gamma-glutamyltransferase (gamma-GT), on gamma-GT activity and apoptosis in 2 human T-lymphoblastoid CEM cell lines, CCRF and VBL-100. In both cell lines, acivicin was found to cause morphological and biochemical changes of apoptosis in a dose...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910620414
更新日期：1995-08-09 00:00:00
abstract：:The age-adjusted incidence rates of cutaneous melanoma in Queensland in 1987 have been analysed for 16 anatomic sites, taking into account their surface areas. In men, the incidence of invasive melanoma on the ears, a chronically sun-exposed site, was extraordinarily high with annual rates of over 200 per 10(5) units ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910530210
更新日期：1993-01-21 00:00:00
abstract：:UDP-glucuronosyltransferase 1A1 (UGT1A1) is an enzyme which catalyses not only the glucuronidation of tobacco smoke carcinogens like benzopyrene, but also of the endogenous substrate bilirubin. Bilirubin for a long time was considered to be only a toxic waste product of hemoglobin degradation, but recent findings have...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25296
更新日期：2010-12-15 00:00:00
abstract：:The antigen-induced leukocyte adherence inhibition (LAI) test is an assay of cellular immune reactivity which was expected to offer a promising adjunct to currently available diagnostic and monitoring procedures in cancer patients. We have applied this assay to (1) 83 inpatients clinically suspected of having breast c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910290306
更新日期：1982-03-15 00:00:00
abstract：:G-protein-coupled receptor (GPCR)-stimulated androgen-independent activation of androgen receptor (AR) contributes to acquisition of a hormone-refractory phenotype by prostate cancer. We previously reported that regulator of G-protein signaling (RGS) 2, an inhibitor of GPCRs, inhibits androgen-independent AR activatio...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.26138
更新日期：2012-04-01 00:00:00
abstract：:Under the aegis of EUROCARE, a European Union project to assemble survival data from population-based cancer registries and analyze them according to standardized procedures, we have investigated and compared colon-cancer survival in 10 European countries. We analyzed 68,283 colon-cancer cases diagnosed between 1978 a...
journal_title：International journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1002/ijc.2910630109
更新日期：1995-09-27 00:00:00
abstract：:The 26S proteasome, consisting of the 20S core and 19S regulatory complexes, regulates intracellular protein concentration through proteolytic degradation of targeted substrates. Composition of the 19S regulatory complex as well as posttranslational modifications of the 19S subunits can effectively regulate the activi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20261
更新日期：2004-09-01 00:00:00
abstract：:Endometrial carcinoma (EC) is traditionally characterized as endometrioid and nonendometrioid based on histopathologic phenotypes. Molecular-based classifications have been proposed, but are not widely implemented. Herein we examine molecular profiles between EC histologic subtypes. 3133 ECs were submitted between Mar...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30537
更新日期：2017-03-15 00:00:00
abstract：:Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase overexpressed in tumors, which plays a major role in the first step of pro-MMP-2 activation, leading to the generation of an intermediate 62 kDa species. The second step of MMP-2 activation that yields to the mature form is less understoo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10134
更新日期：2002-03-01 00:00:00
abstract：:We analyzed the number of circulating tumor cells (CTCs) and Epstein-Barr virus DNA (EBV DNA) for diagnosis, monitoring and prognosis of patients with metastatic nasopharyngeal carcinoma (mNPC). The levels of CTCs and EBV DNA were measured at baseline and after first-line chemotherapy in 148 mNPC patients prospectivel...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32380
更新日期：2019-11-15 00:00:00
abstract：:To estimate the annual incidence of adult T-cell leukemia/lymphoma (ATL) by district in Japan, a large-scale nationwide survey of ATL and of non-Hodgkin's lymphoma was performed in 1988. Questionnaires for the registration of ATL and of T-cell and non-T-cell lymphoma were distributed to the physicians in charge of thi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910450206
更新日期：1990-02-15 00:00:00
abstract：:The partition-defective 3 (PAR-3) protein is implicated in the development and maintenance of cell polarity and is associated with proteins that mediate the changes in cytoskeleton organization required for cell polarity establishment. In this work, we used two original primary cell lines (R-180 and R-305) derived fro...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28548
更新日期：2014-05-01 00:00:00
abstract：:We investigated whether variants in sex steroid hormone metabolism genes modify the effect of hormone therapy (HT) on endometrial cancer risk in postmenopausal non-Hispanic white women. A nested case-control study was conducted within the California Teachers Study (CTS). We genotyped htSNPs in six genes involved in th...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.26163
更新日期：2012-04-01 00:00:00
abstract：:Gliomas are the most common tumors of the central nervous system and have a grave prognosis. Deletion of chromosome 10p15 is one of the most common chromosomal alterations in gliomas. Recently, a candidate tumor suppressor gene, KLF6, which is mapped to chromosome 10p, was found to be frequently mutated in prostate ca...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11123
更新日期：2003-07-10 00:00:00
abstract：:Cholangiocarcinoma is the second most common primary hepatic cancer, with a rising incidence worldwide. Owing to late diagnosis and limited treatment options, the prognosis for cholangiocarcinoma remains dismal, compelling a search for new treatments. As aspirin exhibits a well-supported chemopreventive effect on comm...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.33323
更新日期：2021-03-15 00:00:00
