Purification and fine characterization of a major allergen from Olea europaea pollen extract.

Abstract:

:Olea europaea (olive) pollen extract was prepared by aqueous extraction and characterized by biochemical and immunochemical methods. Two components, displaying respective mol. wt. of 17,000 and 19,000, were the most reactive allergens, being the doublet (designated Ole e I) recognized by most sera tested. The 19,000 mol. wt. component, purified by conventional biochemical procedure and lectin-affinity chromatography from the Ole e I doublet, was deglycosylated and analyzed by SDS-PAGE and by ELISA inhibition. The results obtained suggest that the 19,000 mol. wt. component represents the glycosylated form of the 17,000 component.

journal_name

Allergy

journal_title

Allergy

authors

De Cesare F,Pini C,Di Felice G,Caiaffa MF,Macchia L,Tursi A,Tinghino R,Palumbo S,Sallusto F,Federico R

doi

10.1111/j.1398-9995.1993.tb00724.x

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

248-54

issue

4

eissn

0105-4538

issn

1398-9995

journal_volume

48

pub_type

杂志文章

相关文献

ALLERGY文献大全
  • Pulmonary function changes in asthmatics associated with low-level SO2 and NO2 air pollution, weather, and medicine intake. An 8-month prospective study analyzed by neural networks.

    abstract::This paper presents the response in subjects with asthma to gaseous air-pollution levels, weather, and medicine intake as identified by principal-component analysis and neural network techniques. Pulmonary function measured by respiratory peak-flow rate in nonallergic asthmatics was associated with ambient, low-level,...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1993.tb02402.x

    authors: Moseholm L,Taudorf E,Frøsig A

    更新日期:1993-07-01 00:00:00

  • Occupational asthma caused by exposure to cyanoacrylate.

    abstract:BACKGROUND:Exposure to acrylates may give rise to rhinitis and asthma in both industrial and domestic environments. The mechanisms underlying these respiratory conditions caused by acrylates remain largely unknown. METHODS:We studied two assembly operators exposed to cyanoacrylate glue who developed rhinitis and asthm...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2001.056005446.x

    authors: Quirce S,Baeza ML,Tornero P,Blasco A,Barranco R,Sastre J

    更新日期:2001-05-01 00:00:00

  • Low vitamin D serum level is inversely associated with eczema in children and adolescents in Germany.

    abstract:BACKGROUND:Recent studies have yielded heterogeneous results regarding the relationship between vitamin D and atopic conditions. The aim of this study was to investigate the association between serum vitamin D level and the prevalence of eczema in German children and adolescents. METHODS:Data were drawn for children a...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12167

    authors: Heimbeck I,Wjst M,Apfelbacher CJ

    更新日期:2013-07-01 00:00:00

  • Th17 cells: new players in asthma pathogenesis.

    abstract::CD4+ T effector lymphocytes are distinguished in different subsets on the basis of their patterns of cytokine secretion. Th1 cells, thank to IFN-γ production, are responsible for cell-mediated immunity against intracellular pathogens, Th2 cells, through the production of IL-4, provide some degree of protection against...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.2011.02576.x

    authors: Cosmi L,Liotta F,Maggi E,Romagnani S,Annunziato F

    更新日期:2011-08-01 00:00:00

  • Comparison of skin tests and RAST for the diagnosis of bee sting allergy.

    abstract::Skin tests and estimation of specific IgE-antibodies by the RAST are evaluated as diagnostic procedures in bee sting hypersensitivity with the followiny extracts. (2) With bee venom results of skin tests and RAST correspond in 82%, with bee wholebody extracts in 68%. (3) Both with skin tests and the RAST with bee veno...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1979.tb01705.x

    authors: Patrizzi R,Müller U,Yman L,Hoigné R

    更新日期:1979-08-01 00:00:00

  • Montelukast 10 mg improves nasal function and nasal response to aspirin in ASA-sensitive asthmatics: a controlled study vs placebo.

    abstract:UNLABELLED:Aspirin-induced asthma (AIA) is a clinical syndrome characterized by acute airway reaction to aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDS). The most recent etiological hypothesises is that an overexpression of the enzyme LTC(4) synthase occurs in AIA, with the consequent production of sulf...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1398-9995.2003.00351.x

    authors: Micheletto C,Tognella S,Visconti M,Pomari C,Trevisan F,Dal Negro RW

    更新日期:2004-03-01 00:00:00

  • Fexofenadine reduces nasal congestion in perennial allergic rhinitis.

    abstract:BACKGROUND:Nasal congestion is the predominant symptom in perennial allergic rhinitis (PAR), and it seems to be mainly related to the late-phase inflammatory events. The present pilot study aimed to evaluate the therapeutic effect exerted by fexofenadine in patients with PAR due to mite allergy. METHODS:This study was...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1034/j.1398-9995.2001.00191.x

    authors: Ciprandi G,Cosentino C,Milanese M,Mondino C,Canonica GW

    更新日期:2001-11-01 00:00:00

  • A polymorphism in the TH 2 locus control region is associated with changes in DNA methylation and gene expression.

    abstract:BACKGROUND:Genomewide association and epigenetic studies found a region within the RAD50 gene on chromosome 5q31 to be associated with total serum IgE levels and asthma. In mice, this region harbors a locus control region for nearby TH 2 cytokines, which is characterized by four Rad50 DNase I hypersensitive sites (RHS4...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12450

    authors: Schieck M,Sharma V,Michel S,Toncheva AA,Worth L,Potaczek DP,Genuneit J,Kretschmer A,Depner M,Dalphin JC,Riedler J,Frei R,Pekkanen J,Tost J,Kabesch M

    更新日期:2014-09-01 00:00:00

  • IgE concentrations measured by PRIST in serum of healthy adults and in patients with respiratory allergy. A diagnostic approach.

    abstract::In order to establish reference values for total serum IgE in an adult non-atopic population, 175 individuals, 17-85 years of age, were investigated. The usefulness of a sensitive method (PRIST) for serum IgE determinations in discriminating atopy from non-atopic conditions in allergological routine diagnosis was eluc...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1981.tb01871.x

    authors: Zetterström O,Johansson SG

    更新日期:1981-11-01 00:00:00

  • Paving the way of systems biology and precision medicine in allergic diseases: the MeDALL success story: Mechanisms of the Development of ALLergy; EU FP7-CP-IP; Project No: 261357; 2010-2015.

    abstract::MeDALL (Mechanisms of the Development of ALLergy; EU FP7-CP-IP; Project No: 261357; 2010-2015) has proposed an innovative approach to develop early indicators for the prediction, diagnosis, prevention and targets for therapy. MeDALL has linked epidemiological, clinical and basic research using a stepwise, large-scale ...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/all.12880

    authors: Bousquet J,Anto JM,Akdis M,Auffray C,Keil T,Momas I,Postma DS,Valenta R,Wickman M,Cambon-Thomsen A,Haahtela T,Lambrecht BN,Lodrup Carlsen KC,Koppelman GH,Sunyer J,Zuberbier T,Annesi-Maesano I,Arno A,Bindslev-Jensen C

    更新日期:2016-11-01 00:00:00

  • Single-dose inhaled budesonide in subjects with chronic asthma.

    abstract::In 30 stable asthmatics, a comparison was made between the changes in pulmonary function (FEV1, FVC, PEF, MEF75, MEF50 and MEF25) hourly for 9 h after a single dose of inhaled budesonide 1,600 micrograms, and placebo. All subjects used inhaled steroids daily; this medication was, however, withheld 8 days prior to the ...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1991.tb00619.x

    authors: Engel T,Dirksen A,Heinig JH,Nielsen NH,Weeke B,Johansson SA

    更新日期:1991-10-01 00:00:00

  • Allergic respiratory disease: Different allergens, different symptoms.

    abstract:BACKGROUND:Spanish population is rather homogeneous in its genetic and sociocultural characteristics, but allergen sensitization shows wide geographical variations. We aimed at assessing whether sensitization to different allergens in the diverse geographical areas induced different clinical and quality-of-life charact...

    journal_title:Allergy

    pub_type: 杂志文章,多中心研究

    doi:10.1111/all.13141

    authors: Valero A,Quirce S,Dávila I,Delgado J,Domínguez-Ortega J

    更新日期:2017-09-01 00:00:00

  • Priming and inducing effects of interleukin-3 on histamine release from cord-blood basophils.

    abstract::The effect of interleukin-3 (IL-3) on histamine release from cord and adult blood basophils were evaluated. Leukocyte suspensions, obtained from adult patients with respiratory allergy (n = 15), normal adult subjects (n = 15), and neonates with (n = 15) and without (n = 19) atopic disposition, were stimulated with ant...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1997.tb02419.x

    authors: Miadonna A,Salmaso C,Marco MP,Pugni L,Milazzo N,Tedeschi A,Marini A

    更新日期:1997-10-01 00:00:00

  • Weight loss interventions in asthma: EAACI evidence-based clinical practice guideline (part I).

    abstract:BACKGROUND:Asthma and obesity are chronic multifactorial conditions that are associated with gene-environment interaction and immune function. Although the data are not fully consistent, it seems that obesity increases the risk of asthma and compromises asthma control. OBJECTIVE:To investigate the impact that weight c...

    journal_title:Allergy

    pub_type: 杂志文章,meta分析

    doi:10.1111/all.12106

    authors: Moreira A,Bonini M,Garcia-Larsen V,Bonini S,Del Giacco SR,Agache I,Fonseca J,Papadopoulos NG,Carlsen KH,Delgado L,Haahtela T

    更新日期:2013-04-01 00:00:00

  • Proteome analysis identifies L1CAM/CD171 and DPP4/CD26 as novel markers of human skin mast cells.

    abstract:BACKGROUND:The function of skin mast cells has been well documented in IgE-mediated allergic reactions, whereas other mast cell functions are poorly defined. This study aimed at identifying novel mast cell proteins by proteome analysis of primary human skin mast cells. METHODS:The proteome of skin mast cells was compa...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12919

    authors: Gschwandtner M,Paulitschke V,Mildner M,Brunner PM,Hacker S,Eisenwort G,Sperr WR,Valent P,Gerner C,Tschachler E

    更新日期:2017-01-01 00:00:00

  • The influence of air conditioning, humidity, temperature and other household characteristics on mite allergen concentrations in the northeastern United States.

    abstract:BACKGROUND:Information about the influence of housing and occupant characteristics on mite allergen concentrations is crucial to determine which methods could be used to decrease exposure of susceptible subjects. OBJECTIVES:To identify housing and occupant characteristics that are associated with mite allergen concent...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2004.00470.x

    authors: van Strien RT,Gehring U,Belanger K,Triche E,Gent J,Bracken MB,Leaderer BP

    更新日期:2004-06-01 00:00:00

  • Comparative immunophenotyping of monocytes from symptomatic and asymptomatic atopic individuals.

    abstract:BACKGROUND:Allergy has at least two components - a genetic predisposition referred to as atopy and the progress from an atopic state to clinically apparent disease. Peripheral blood monocytes are circulating myeloid precursors of antigen-presenting cells. The expression of cell surface proteins on monocytes may therefo...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2004.00546.x

    authors: von Bubnoff D,Scheler M,Hinz T,Matz H,Koch S,Bieber T

    更新日期:2004-09-01 00:00:00

  • Atopy and intolerance of antimicrobial drugs increase the risk of reactions to acetaminophen and nimesulide in patients allergic to nonsteroidal anti-inflammatory drugs.

    abstract::This study evaluated the risk factors for developing allergic reactions to alternative drugs such as acetaminophen and nimesulide in 367 patients intolerant of nonsteroidal anti-inflammatory drugs (NSAID) compared to 243 healthy controls. All subjects were given test doses (TD) of acetaminophen and nimesulide, and age...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1998.tb03995.x

    authors: Pastorello EA,Zara C,Riario-Sforza GG,Pravettoni V,Incorvaia C

    更新日期:1998-09-01 00:00:00

  • Treatment of mast cells with carbon dioxide suppresses degranulation via a novel mechanism involving repression of increased intracellular calcium levels.

    abstract:BACKGROUND:Intranasal noninhaled delivery of carbon dioxide (CO₂) is efficacious in the symptomatic treatment of seasonal allergic rhinitis. The goal of this study was to determine whether and how 100% CO₂ inhibits mast cell degranulation, thereby possibly contributing to the reduction of symptoms in seasonal allergic ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2010.02482.x

    authors: Strider JW,Masterson CG,Durham PL

    更新日期:2011-03-01 00:00:00

  • Helminths can protect themselves against rejection inhibiting hostile respiratory allergy symptoms.

    abstract::The ability of common environmental allergens to stimulate IgE responses and thus to produce allergic diseases has tended to overshadow the fact that helminthic parasites are possibly the most potent inducers of this immunoglobulin that exists in nature. Although it has been well established that during these infectio...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.2006.00983.x

    authors: Mingomataj EC,Xhixha F,Gjata E

    更新日期:2006-04-01 00:00:00

  • Artemisia annua-sublingual immunotherapy for seasonal allergic rhinitis: A randomized controlled trial.

    abstract:BACKGROUND:Artemisia annua is an important autumnal pollen allergen for seasonal allergic rhinitis (SAR) in northern China. To date, no study has investigated allergen immunotherapy with A annua. We aimed to investigate the efficacy and mechanisms underlying A annua-sublingual immunotherapy (SLIT). METHODS:This was a ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.14218

    authors: Lou H,Huang Y,Ouyang Y,Zhang Y,Xi L,Chu X,Wang Y,Wang C,Zhang L

    更新日期:2020-08-01 00:00:00

  • The role of hen's egg-specific IgE, IgG and IgG4 in the diagnostic procedure of hen's egg allergy.

    abstract:BACKGROUND:Hen's egg (HE) allergy is a common disease in childhood. HE-specific serum IgE has been correlated with the outcome of oral food challenge tests, and diagnostic decision points have been described as helpful but still not sufficient to reduce the requirement for oral food challenges. The aim of the study was...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2010.02429.x

    authors: Ahrens B,Lopes de Oliveira LC,Schulz G,Borres MP,Niggemann B,Wahn U,Beyer K

    更新日期:2010-12-01 00:00:00

  • Sublingual immunotherapy with a standardized cat dander extract: evaluation of efficacy in a double blind placebo controlled study.

    abstract:BACKGROUND:Little information is available on the clinical efficacy of sublingual immunotherapy (SLIT) using extracts derived from mammalian epithelia. OBJECTIVES:To assess clinical efficacy of cat SLIT based on natural exposure challenge test (NCT). MATERIAL AND METHODS:Fifty cat allergic patients with rhinoconjunct...

    journal_title:Allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.2007.01365.x

    authors: Alvarez-Cuesta E,Berges-Gimeno P,González-Mancebo E,Fernández-Caldas E,Cuesta-Herranz J,Casanovas M

    更新日期:2007-07-01 00:00:00

  • Increased allergen concentration enhances IFN-gamma production by allergic donor T cells expressing a peripheral tissue trafficking phenotype.

    abstract:BACKGROUND:Clinically effective allergen-specific immunotherapy correlates with decreased circulating allergen-specific IL-4+ T cells but increased IFN-gamma+ cells at sites of allergen challenge. Whether immunotherapy promotes trafficking of IFN-gamma+ T cells to peripheral tissues is unknown. As aeroallergen is admin...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2004.00584.x

    authors: Gardner LM,Spyroglou L,O'Hehir RE,Rolland JM

    更新日期:2004-12-01 00:00:00

  • Protective effect of different doses of terfenadine on the conjunctival provocation test.

    abstract::The protective effect of terfenadine on inflammatory processes following the early phase of conjunctival provocation tests by specific allergen was assessed in 24 patients suffering from seasonal allergic rhinoconjunctivitis, in a single-blind, randomized, placebo-controlled, parallel-group study. Patients were random...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1992.tb02059.x

    authors: Ciprandi G,Buscaglia S,Iudice A,Canonica GW

    更新日期:1992-08-01 00:00:00

  • Bronchial responsiveness to bakery-derived allergens is strongly dependent on specific skin sensitivity.

    abstract:BACKGROUND:Quantitative relationships between immunological reactivity, non-specific bronchial responsiveness and bronchial responsiveness to allergens have scarcely been investigated in occupational asthma. METHODS:We assessed the above relationships in 24 subjects with baker's asthma. The skin endpoint titration to ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2006.01189.x

    authors: Quirce S,Fernández-Nieto M,Escudero C,Cuesta J,de Las Heras M,Sastre J

    更新日期:2006-10-01 00:00:00

  • Milk whey-specific immune complexes in allergic and non-allergic subjects.

    abstract::We developed an antigen- and isotype-specific ELISA for the rapid detection of native serum immune complexes (IC). It is a sandwich assay, in which a solid phase antigen-specific capture antibody selectively binds the antigen-specific IC via the antigen. The isotype of the bound IC is then identified using an enzyme-l...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1988.tb01626.x

    authors: Bell SJ,Potter PC

    更新日期:1988-10-01 00:00:00

  • Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations.

    abstract:BACKGROUND:The function of eosinophils has been attributed to host defense, immunomodulation, and fibrosis. Although eosinophils are found among infiltrating cells in a broad spectrum of skin diseases, their pathogenic role remains uncertain. This study aimed to analyze the cytokine expression by eosinophils in differe...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2011.02694.x

    authors: Roth N,Städler S,Lemann M,Hösli S,Simon HU,Simon D

    更新日期:2011-11-01 00:00:00

  • Crossed Immunoelectrophoresis analysis of bee venom. Enzymatic identification of antigens.

    abstract::Bee venom obtained by electrical stimulation has been analysed by crossed immunoelectrophoresis against high-titer rabbit antibodies. The antigenic analysis of bee venom revealed that the extract contained 17 antigens, which were detected in the crossed immunoelectrophoresis pattern after staining with Coomassie Brill...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1982.tb01910.x

    authors: Einarsson R,Annerhed A,Karlsson R,Olsson B,Renck B

    更新日期:1982-05-01 00:00:00

  • Heat-labile Escherichia coli toxin enhances the induction of allergen-specific IgG antibodies in epicutaneous patch vaccination.

    abstract::Epicutaneous allergen-specific immunotherapy (EPIT) is proposed as an alternative route for allergen-specific immunotherapy (AIT). The induction of allergen-specific blocking IgG antibodies represents an important mechanism underlying AIT, but has not been investigated for EPIT. Here, we compared the induction of alle...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.13036

    authors: Cabauatan CR,Campana R,Niespodziana K,Reinisch C,Lundberg U,Meinke A,Henning R,Neubauer A,Valenta R

    更新日期:2017-01-01 00:00:00