Abstract:
:The carcinogenic heterocyclic amine 2-amino-3,8-dimethyl-imidazo[4,5-f]quinoxaline (MeIQx) is widely distributed in cooked foods. The nuclease P1 method increased the sensitivity of the standard 32P-postlabeling analysis about 1000-fold for detection of MeIQx-DNA adducts. The recovery of MeIQx-DNA adducts by the nuclease P1 method was determined to be about 50% using liver DNA of a rat treated with [14C]MeIQx intragastrically. By the nuclease P1 method five adducts were detected in the liver DNA of rats fed MeIQx and two of them, including the most abundant one, were identified as MeIQx-deoxyguanosine adducts by comparison with the adducts formed in in vitro reactions of N-acetoxy-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline with the four 2'-deoxyribonucleotides. The most abundant adduct in vivo was identified as N2-(deoxyguanosin-8-yl)-MeIQx 3',5'-diphosphate (3',5'-pdGp-C8-MeIQx). MeIQx-DNA adduct levels in human tissues could be determined by the nuclease P1 modification of the 32P-postlabeling method in combination with HPLC, and thus provide information on the roles of MeIQx in human carcinogenesis.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Ochiai M,Nagaoka H,Wakabayashi K,Tanaka Y,Kim SB,Tada A,Nukaya H,Sugimura T,Nagao Mdoi
10.1093/carcin/14.10.2165subject
Has Abstractpub_date
1993-10-01 00:00:00pages
2165-70issue
10eissn
0143-3334issn
1460-2180journal_volume
14pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::The tumor suppressor PTEN antagonizes phosphatidylinositol 3-kinase (PI3K), which contributes to tumorigenesis in many cancer types. While PTEN mutations occur in some melanomas, their precise mechanistic consequences have yet to be elucidated. We sought to identify novel downstream effectors of PI3K using a combinati...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl016
更新日期:2006-09-01 00:00:00
abstract::In light of clinical and biological evidence that bile constituents exert preventive effects against colorectal cancer, we evaluated the influence of oral bilirubin and sodium taurocholate (NaTC) on intestinal tumor formation in APC(Min/+) mice. Mice received bilirubin and/or bovine serum albumin (BSA) and NaTC in the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq050
更新日期:2010-06-01 00:00:00
abstract::Caucasian renal transplant recipients from Queensland, Australia have the highest non-melanoma skin cancer (NMSC) risk worldwide. Although ultraviolet light (UVR) exposure is critical, genetic factors also appear important. We and others have shown that polymorphism in the glutathione S-transferases (GST) is associate...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh291
更新日期:2005-01-01 00:00:00
abstract::Using an initiation--selection--promotion protocol for induction of liver tumors in Wistar rats, the modulating action of various peroxisome proliferators on neoplasia as well as on selected biochemical parameters was studied. After treatment with diethylnitrosamine (DEN), the animals were subsequently subjected to a ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.11.1899
更新日期:1990-11-01 00:00:00
abstract::The human homolog of the Drosophila headcase (HECA) belongs to a new class of cell differentiation regulators. In Drosophila, the HECA protein regulates the proliferation and differentiation of cells during adult morphogenesis. There is growing evidence that HECA plays an important role in human carcinogenesis. In dif...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp189
更新日期:2009-10-01 00:00:00
abstract::Semi-permeable magnetic microcapsules previously shown able to trap gastrointestinal carcinogens and containing polyethyleneimine (PEI) were covalently labelled with [14CH3], and administered for the first time to humans (six healthy volunteers, 1.3 microCi/dose) in gelatin capsules together with radio-opaque gut tran...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.4.683
更新日期:1992-04-01 00:00:00
abstract::The resistant hepatocyte model was used to study expression of tumor-associated aldehyde dehydrogenase (ALDH) activity during the course of rat hepatocarcinogenesis. The hepatic ALDH phenotype was determined at intervals over 280 days by histochemical analysis, total ALDH activity assays and gel electrophoresis, using...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.12.1679
更新日期:1984-12-01 00:00:00
abstract::Changes in lipid metabolism were monitored in rat hepatocyte nodules at certain time points over 9 months. Tissue obtained from partially hepatectomized rats, collected over a period of 7 days, were included as a control for normal hepatocyte cell proliferation. Two important features regarding the lipid profiles of h...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.5.795
更新日期:2001-05-01 00:00:00
abstract::Male and female strain A/J mice were exposed to a mixture of cigarette sidestream and mainstream smoke at a chamber concentration of total suspended particulates of 82.5 mg/m3. Exposure time was 6 h/day, 5 days/week for 5 months. The animals were allowed to recover for another 4 months in filtered air before sacrifice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.10.1789
更新日期:1998-10-01 00:00:00
abstract::5,9-Dimethyldibenzo[c,g]carbazole (DMDBC), a potent mouse hepatocarcinogen, has been shown to induce a non-linear increase in mutant frequency in the liver of the transgenic MutaMouse. To gain insight into the mechanisms underlying the mutagenicity of DMDBC in vivo, DNA damage formation and removal were monitored in m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.2.289
更新日期:2000-02-01 00:00:00
abstract::Transforming growth factor beta1 (TGFbeta1) expression is elevated by tumor promoters in the mouse skin, but its role in tumor promotion has not been well defined. To investigate this, we have compared TGFbeta1+/+ and +/- mice in a two-stage skin chemical carcinogenesis protocol. Surprisingly, TGFbeta1+/- mice had few...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq041
更新日期:2010-06-01 00:00:00
abstract::Species differences in the metabolism of toxic chemicals can confound the extrapolation of experimentally determined risk data to man. However, it is often difficult to obtain reliable information on human metabolism, particularly of genotoxic agents. In this study, comparisons of chromatographic profiles of DNA adduc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.6.965
更新日期:1986-06-01 00:00:00
abstract::The rates of formation and disappearance of benzo[a]pyrene (B[a]P) DNA-adducts were analyzed in the epidermis of SENCAR mice over a 21-day time course. Mice were treated topically with 200 nmol/mouse of [3H]B[a]P at various times prior to sacrifice. The formation and disappearance of total adducts as well as individua...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.5.741
更新日期:1985-05-01 00:00:00
abstract::Zinc has been shown to be accumulated in N-methyl-N-nitrosourea (MNU)-induced rat mammary tumors. Zinc is required for cell proliferation and tumorigenesis is characterized by dysregulation of cell proliferation. An accumulation of zinc in mammary tumors perhaps indicates a reliance on zinc to sustain tumor growth. Li...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh214
更新日期:2004-10-01 00:00:00
abstract::The effect of a deoxycholic acid instillation on colonic epithelium was investigated and compared with the effect of an intervention scheme in which deoxycholic acid (DCA) treated mice received oral supplements of calcium lactate. The morphology of colonic tissue exposed to DCA was markedly affected when compared to t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.9.1205
更新日期:1983-09-01 00:00:00
abstract::The aims of this study were to examine the anti-proliferative effects of different concentrations of a commercial preparation of conjugated linoleic acids (CLA) mixture of isomers [cis-9, trans-11 CLA (c9,t11 CLA): trans-10, cis-12 CLA (50:50)] and their constituent isomers on PC-3, a human prostatic carcinoma cell li...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh116
更新日期:2004-07-01 00:00:00
abstract::The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Int...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.12.2335
更新日期:1989-12-01 00:00:00
abstract::The enzyme O6-methylguanine-DNA methyltransferase repairs alkylation-induced DNA damage, O6-methylguanine and O4-methylthymine, the former being formed more frequently. Previously, by means of gene targeting, we generated mice in which alleles for methyltransferase were disrupted. We now use these mouse lines, which a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.8.1631
更新日期:1997-08-01 00:00:00
abstract::Environmental contributions to cancer development are widely accepted, but only a fraction of all pertinent exposures have probably been identified. Traditional toxicological approaches to the problem have largely focused on the effects of individual agents at singular endpoints. As such, they have incompletely addres...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgv037
更新日期:2015-06-01 00:00:00
abstract::The expression of proteinase-activated receptor (PAR)(2) in human hepatocellular carcinoma (HCC) was established by reverse transcription-polymerase chain reaction, confocal immunofluorescence and electron microscopy in permanent cell lines, primary HCC cell cultures and HCC tumor tissue. Stimulation of HCC cells with...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp153
更新日期:2009-09-01 00:00:00
abstract::Retinoids have been shown to exert an anticarcinogenic effect through suppression of the cell cycle, induction of apoptosis and/or differentiation. In rat liver, in particular, retinoic acid has been shown to inhibit regeneration after partial hepatectomy, most probably through repression of the expression of c-fos an...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh221
更新日期:2004-11-01 00:00:00
abstract::Although microRNAs (miRNA) have extensively been investigated in cancer research, less attention has been paid to their regulation by carcinogens and/or protective factors in early stages of the carcinogenesis process. The present study was designed to evaluate the modulation of mRNA expression as related to exposure ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq037
更新日期:2010-05-01 00:00:00
abstract::Human fibroblasts repair DNA damaged by bleomycin through both short-patch and long-patch pathways, mediated by an aphidicolin-resistant (beta) and aphidicolin-sensitive (delta) DNA polymerase respectively (DiGiuseppe, J.A. and Dresler, S.L. (1989) Biochemistry, 28, 9515-9520). Despite certain similarities, aphidicoli...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.6.1021
更新日期:1990-06-01 00:00:00
abstract::Spindle cell carcinomas were identified using polyacrylamide gel electrophoresis and immunoblotting of proteins extracted from paraffin-embedded tissue sections. Immunohistochemistry using rabbit monospecific antisera against the mouse 55 kd keratin polypeptide also identified these tumors. A group of 53 SENCAR mice i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.11.2169
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:The hepatitis B virus x gene (HBx) is a promiscuous transactivator implicated in the development of hepatocellular carcinoma (HCC). The present study was designed to investigate the molecular events regulated by HBx. METHODS:Genomic and proteomic expression profiling was performed in Huh7 HCC cells transfec...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn111
更新日期:2008-06-01 00:00:00
abstract::Amarogentin, a secoiridoid glycoside, is an active component of the medicinal plant Swertia chirata. In this study, chemopreventive and chemotherapeutic actions of amarogentin were evaluated in a carbon tetrachloride (CCl(4))/N-nitrosodiethylamine (NDEA)-induced liver carcinogenesis mouse model system during continuou...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs276
更新日期:2012-12-01 00:00:00
abstract::Alcohol is a major risk factor for oesophageal squamous cell carcinoma (OSCC), the most prevalent histological subtype of oesophageal cancer (OC) worldwide. The metabolism of alcohol is regulated by specific enzymes whose activity and expression is influenced by genetic polymorphisms. We conducted a systematic review ...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgx067
更新日期:2017-09-01 00:00:00
abstract::The carcinogenicities of 1-nitropyrene (1-NP), 4-nitropyrene (4-NP), 1,3-dinitropyrene (1,3-DNP), 1,6-dinitropyrene (1,6-DNP), 1,8-dinitropyrene (1,8-DNP), 3-hydroxy-1-nitropyrene (3-OH-1-NP) and a mixture of 6- and 8-hydroxy-1-nitropyrene (6/8-OH-1-NP) were investigated in newborn female rats. Newborn female CD rats ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.12.3027
更新日期:1995-12-01 00:00:00
abstract::Angiogenesis, the formation of new blood vessels, is critical to tumor growth and metastasis. Vascular endothelial growth factor (VEGF), an important angiogenic activator, is essential for angiogenesis. Our laboratory has used a rodent model of human esophageal squamous cell carcinoma (ESCC) to identify putative chemo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl109
更新日期:2006-11-01 00:00:00
abstract::We previously reported that quinacrine (QC) has anticancer activity against breast cancer cells. Here, we examine the mechanism of action of QC and its ability to inhibit Wnt-TCF signaling in two independent breast cancer cell lines. QC altered Wnt-TCF signaling components by increasing the levels of adenomatous polyp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs351
更新日期:2013-02-01 00:00:00