An analysis of actin isoforms expressed in hair-cell enriched fractions of the chick basilar papilla by the polymerase chain reaction technique.

Abstract:

:Actin mRNA was characterised in hair-cell enriched fractions of the chick basilar papilla, by means of the reverse-transcription polymerase chain reaction technique. Primers were directed against the 3' untranslated portions of the actin mRNAs. Evidence for beta-cytoplasmic and gamma-cytoplasmic actin mRNA was found; no evidence was found for alpha-skeletal, alpha-cardiac or type 5 cytoplasmic actin mRNAs. Since beta-actin is known to form bundles of filaments whereas gamma-actin does not, this suggests that the hair-cell stereocilia are composed of beta-actin.

journal_name

Hear Res

journal_title

Hearing research

authors

Pickles JO

doi

10.1016/0378-5955(93)90038-3

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

225-9

issue

1-2

eissn

0378-5955

issn

1878-5891

pii

0378-5955(93)90038-3

journal_volume

71

pub_type

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