Abstract:
:We have developed a human artificial episomal chromosome (HAEC) system, based on the latent replication origin of the large herpes Epstein-Barr virus, for the propagation and stable maintenance of DNA as circular minichromosomes in human cells. Individual HAECs carried human genomic inserts ranging from 60-330 kb and appeared genetically stable. An HAEC library of 1,500 independent clones carrying random human genomic fragments with average sizes of 150-200 kb was established and allowed recovery of the HAEC DNA. Our autologous HAEC system, with human DNA cloned directly in human cells, provides an important tool for functional study of large mammalian DNA regions and gene therapy.
journal_name
Nat Genetjournal_title
Nature geneticsauthors
Sun TQ,Fenstermacher DA,Vos JMdoi
10.1038/ng0994-33subject
Has Abstractpub_date
1994-09-01 00:00:00pages
33-41issue
1eissn
1061-4036issn
1546-1718journal_volume
8pub_type
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