Abstract:
BACKGROUND/AIMS:Hepatic glucuronidation is quantitatively the most important conjugation reaction by which an array of endogenous compounds and xenobiotics undergo biotransformation and detoxification. The active site of the uridine diphosphate (UDP) glucuronosyltransferases, which catalyze glucuronidation reactions, has been postulated to reside in the lumen of the endoplasmic reticulum. The aim of this study was to characterize the process whereby UDP glucuronic acid (UDP-GlcUA), the cosubstrate for all glucuronidation reactions, is transported into microsomal vesicles. METHODS:The uptake process was analyzed using rapid filtration techniques, radiolabeled UDP-GlcUA, and rat liver microsomes. RESULTS:Uptake was saturable with respect to time and concentration, inhibited by 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid and 4-acetamido-4'-isothio-cyanatostilbene-2-2'-disulfonic acid, and was osmotically sensitive. Transport was stimulated by Mg2+ and guanosine triphosphate (50 mumol/L) but not guanosine 5'-O-(3-thiotriphosphate) or adenosine triphosphate. Luminal UDP-N-acetylglucosamine (1 mmol/L) produced enhanced uptake of UDP-GlcUA (trans stimulation). In contrast to nucleotide sugar transport in the Golgi apparatus, trans uridine monophosphate and UDP did not alter UDP-GlcUA transport in microsomes, indicating distinct processes. CONCLUSIONS:These data provide unambiguous evidence for the existence of a unique, substrate-specific, regulated, carrier-mediated process that transports UDP-GlcUA into the lumen of hepatocyte microsomes. This transporter may regulate glucuronidation in vivo.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Berg CL,Radominska A,Lester R,Gollan JLdoi
10.1016/0016-5085(95)90023-3subject
Has Abstractpub_date
1995-01-01 00:00:00pages
183-92issue
1eissn
0016-5085issn
1528-0012pii
0016-5085(95)90023-3journal_volume
108pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Chronic hepatitis C virus infection activates an intrahepatic immune response, leading to increased expression of interferon (IFN)-stimulated genes and activation of natural killer (NK) cells-the most prevalent innate immune cell in the liver. We investigated whether the elimination of hepatitis C vir...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.03.004
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND & AIMS:Intrahepatic bile ducts express a range of lymphocyte adhesion molecules during episodes of inflammation. This may contribute to the susceptibility of biliary epithelium to immunologic damage during liver allograft rejection, graft-versus-host disease, and primary biliary cirrhosis. However, no previo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v112.pm9041260
更新日期:1997-03-01 00:00:00
abstract::Ketoconazole has only recently been recognized as a cause of hepatic injury, with most reports coming from outside the United States. In order to characterize more fully the U.S. experience, we undertook an analysis of 54 reports of alleged ketoconazole-induced liver injury submitted to the Food and Drug Administratio...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND & AIMS:In experimental animals, the indigenous microbiota modulates mucosal immunity. In humans, such direct evidence is scarce. The aim of this study was to examine the effect of intestinal bacteria on the local immunoglobulin (Ig) response. METHODS:The numbers of IgA-, IgM-, and IgG-producing immunocytes ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90389-5
更新日期:1995-09-01 00:00:00
abstract::The effect of deoxycholate ingestion, 750 mg per day, on bile acid kinetics, biliary bile acid composition, and biliary lipid secretion was studied in 7 healthy volunteers. Bile acid kinetics were measured by isotope dilution, and hourly outputs of bile acid, cholesterol, and phospholipid were quantitated by a duodena...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,评审
doi:
更新日期:1977-01-01 00:00:00
abstract::We have been able to isolate mycobacteria from intestinal specimens obtained by surgical resection or endoscopic biopsy from patients with Crohn's disease, ulcerative colitis, and noninflammatory bowel diseases. Nineteen slow-growing (Runyon groups I and III) and 17 rapid-growing (Runyon group IV) mycobacterial isolat...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)90139-9
更新日期:1987-02-01 00:00:00
abstract:BACKGROUND & AIMS:Alcoholic hepatitis (AH) is characterized by hepatocellular damage, inflammation, and fibrosis. We performed a prospective study to associate hepatic expression of the CXC subfamily of chemokines with histology findings and prognosis of patients with AH. METHODS:Liver biopsy samples from 105 patients...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.01.056
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND/AIMS:Conventional flow-cytometric analysis of colorectal tumor DNA content includes both tumor and stromal cell DNA. To improve the method, we evaluated the DNA content of purified epithelial cells using the crypt-isolation technique. METHODS:Normal and neoplastic crypts were isolated from fresh resected co...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(94)94651-5
更新日期:1994-01-01 00:00:00
abstract::Regional specific antibodies and chromatography were used to analyze the distributions and molecular forms of peptide histidine isoleucine (PHI) and vasoactive intestinal peptide (VIP) in the porcine intestine. Both peptides were present along the entire length of the intestine, the highest concentrations occurring in...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90445-7
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND & AIMS:Because current therapies for chronic hepatitis C virus (HCV) infections are suboptimal and associated with severe side effects, novel treatment options are needed. A small animal model has recently been developed to study HCV infections. To examine the usefulness of this human liver-urokinase-type pl...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.07.007
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND & AIMS:Activation of Fas or tumor necrosis factor receptor 1 (TNF-R1) on hepatocytes leads to apoptosis, which requires mitochondria activation. The pro-death Bcl-2 family protein, Bid, mediates this pathway by inducing mitochondrial releases of cytochrome c and other apoptotic factors. How Bid activates mit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(03)01066-7
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND & AIMS:We have shown previously that cure of Helicobacter pylori infection leads to the disappearance of acid-neutralizing substances. Also, patients with ulcer after cure may gain weight. The aim of this study was to investigate whether cure of the infection increases the risk of reflux esophagitis. METHOD...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1016/s0016-5085(97)70024-6
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND & AIMS:Postprandial glycemia excursions increase after gastric bypass surgery; this effect is even greater among patients with recurrent hypoglycemia. These patients also have increased postprandial levels of insulin and glucagon-like peptide 1 (GLP-1). We performed a clinical trial to determine the role of ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2013.11.044
更新日期:2014-03-01 00:00:00
abstract::We tested methyl tertiary butyl ether both in vitro and in vivo to evaluate its efficacy as a potential cholesterol gallstone solvent for direct instillation into the human gallbladder or bile duct. Like diethyl ether, methyl tertiary butyl ether is an aliphatic ether with an excellent cholesterol-solubilizing capacit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(85)80143-8
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND & AIMS:Anemia is associated commonly with acute and chronic inflammation, but the mechanisms of their interaction are not clear. We investigated whether microRNA 122 (MIR122), which is generated in the liver and is secreted into the blood, is involved in the development of anemia associated with inflammation...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2016.07.031
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND & AIMS:Qualitative endoscopic ultrasound (EUS) elastography is an accurate but subjective tool for the differential diagnosis of solid pancreatic masses. Second-generation EUS elastography allows quantitative analysis of tissue stiffness. We evaluated the accuracy of quantitative, second-generation EUS elast...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.06.059
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND & AIMS:Feeding intolerance because of functional gastrointestinal dysmotility and parenteral nutrition-associated cholestasis (PNAC) are common problems in preterm, very-low-birth-weight (VLBW) infants. This double-blind, randomized, placebo-controlled study aimed to assess the effectiveness of "high-dose" o...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2007.03.043
更新日期:2007-05-01 00:00:00
abstract::Gastric and esophageal emptying were assessed using scintigraphic techniques in 12 patients with progressive systemic sclerosis and 22 normal volunteers. Esophageal emptying was significantly delayed in the patient group, with 7 of the 12 patients beyond the normal range. Gastric emptying was slower in patients than i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::A rapid, sensitive, precise radioimmunoassay for conjugates of chenodeoxycholic (chenic) acid has been developed and validated. Immunogen was prepared with chenylglycine couples to bovine serum albumin, emulsified in Freund's complete adjuvant, and injected intracutaneously in rabbits. Antibodies of moderate titer (us...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
abstract::The pathogenesis of gastroesophageal reflux disease (GERD) is complex and involves changes in reflux exposure, epithelial resistance, and visceral sensitivity. The gastric refluxate is a noxious material that injures the esophagus and elicits symptoms. Esophageal exposure to gastric refluxate is the primary determinan...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2017.09.047
更新日期:2018-01-01 00:00:00
abstract::The purpose of this American Gastroenterological Association (AGA) Institute Clinical Practice Update was to rapidly review the emerging evidence and provide timely expert recommendations regarding the management of patients with inflammatory bowel disease during the coronavirus disease 2019 pandemic. This expert comm...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.04.012
更新日期:2020-07-01 00:00:00
abstract::In the previous decade, comprehensive genomic analyses have yielded important insights about the genetic alterations that underlie pancreatic tumorigenesis. Whole-exome and whole-genome sequencing of pancreatic ductal adenocarcinomas have confirmed the critical driver genes altered in the majority of pancreatic cancer...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2018.12.039
更新日期:2019-05-01 00:00:00
abstract::The bioavailability of parenteral cimetidine was tested in 12 volunteers in a balanced three-way crossover study. Blood levels and urinary excretion were compared after intramuscular and intravenous injection and oral administration of 300 mg of cinetidine. The results indicated that the intramuscular and intravenous ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract:BACKGROUND & AIMS:Our aim was to investigate the relationship between alcohol consumption and iron overload, iron deficiency, or iron deficiency anemia in the U.S. population. METHODS:Adult participants of the Third National Health and Nutrition Examination Survey who did not consume alcohol (n = 8839) were compared w...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2004.01.020
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND & AIMS:Dihydroxy bile acids induce a bicarbonate-rich hypercholeresis when secreted into canalicular bile in unconjugated form; the mechanism is cholehepatic shunting. The aim of this study was to identify a xenobiotic that induces hypercholeresis by a similar mechanism. METHODS:Five organic acids (sulindac...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70356-2
更新日期:1999-10-01 00:00:00
abstract::The prevalence of delta-markers among 71 patients with fulminant B viral hepatitis was found to be 33.8%. The majority of the patients with delta-markers showed serologic evidence of simultaneous acute delta-infection and B viral infection. Only 5 of the 24 patients with serologic markers of acute delta-infection in t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::Spinal cord stimulation is an increasingly popular form of pain treatment. An electrode positioned on the dorsal aspect of the spinal cord at the level of the nerve roots from the painful area stimulates the spinal cord. Current from the electrode is supplied by a pulse generator in the lower anterior abdominal wall. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70570-6
更新日期:1999-07-01 00:00:00
abstract::In recent years the hypothesis that the number of villus cells regulates crypt cell proliferation in the epithelium of the small intestine has been brought forward by a number of investigators. To test this hypothesis, the villus cell population was reduced by clamping the superior mesenteric artery and vein in rats f...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::In this review, we compared the outcome of 25 studies of experimentally induced pancreatitis in animals with 13 studies of human acute pancreatitis in which the same therapeutic agents were used (aprotinin, glucagon, 5-fluorouracil, somatostatin, peritoneal lavage). Whereas 81% of the animal studies had a positive out...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,评审
doi:10.1016/0016-5085(87)90275-7
更新日期:1987-12-01 00:00:00
abstract::In vitro studies have shown that some bile salts inhibit the growth of intestinal bacteria. We investigated the possibility that bile salts might have a similar action in vivo in the ileum by performing serial anaerobic cultures and bile salt analyses on postprandial ileal fluid obtained anaerobically via intestinal t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00