Abstract:
:We have demonstrated the formation of entorhinodentate projections by axons arising from explants of embryonic mouse entorhinal cortex or slices of postnatal rat entorhinal area co-cultured in contact with slices of postnatal rat hippocampus in roller tube and static culture. Species-specific markers (Thy-1 alleles and M6) showed that the most dense part of the projection was to the outer part of the molecular layer of the dentate gyrus (i.e. excluding the commissural-association zone). Retrograde axonal transport of fluorescent tracers placed in the dentate gyrus labelled a densely packed superficial layer of stellate cells in the entorhinal cortex. Anterograde axonal transport of biocytin placed in the entorhinal cortex showed that the entorhinodentate fibres formed typical parallel bundles oriented at right angles to the dentate granule cell dendrites and had short-stalked boutons. The formation of entorhinodentate synapses was confirmed in the electron microscope by electron-dense degeneration after cutting the previously formed connection between the co-cultures. Synaptic transmission was demonstrated by extracellular recording of postsynaptic field potentials after entorhinal stimulation. The entorhinal fibres also projected to the hippocampal stratum lacunosum-moleculare of fields CA1 and CA3, and were present in the outer part of the stratum oriens of the subiculum; in some cases they perforated the pyramidal cell layer of the subiculum. We conclude that the necessary molecular and tissue organizational signals for the formation of an entorhinodentate projection are present in tissues maintained in organotypic slice co-culture, and remain effective in the cross-species mouse-to-rat situation.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Li D,Field PM,Starega U,Li Y,Raisman Gdoi
10.1016/0306-4522(93)90530-ssubject
Has Abstractpub_date
1993-02-01 00:00:00pages
799-813issue
4eissn
0306-4522issn
1873-7544pii
0306-4522(93)90530-Sjournal_volume
52pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::In awake, freely moving rats, the intracerebral administration of the excitatory amino acid L-glutamate (30 nmol/0.5 microliters) into discrete regions of the brainstem resulted in a transient and spontaneous pain-like syndrome characterized by an initial vocalization and vigorous escape behavior. Systematic microinje...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90142-o
更新日期:1992-01-01 00:00:00
abstract::Depolarization-induced transmitter release in synaptosomes prepared from the hippocampus of aged rats is decreased compared with release from young animals. Although the underlying cause of this deficit is not known, some evidence suggests that increased membrane rigidity may contribute to these age-related synaptic c...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00219-5
更新日期:1999-01-01 00:00:00
abstract::We have previously described a population of 5-hydroxytryptamine neurons which repetitively fires bursts of usually two (but occasionally three or four) action potentials, with a short (<20 ms) interspike interval within a regular low-frequency firing pattern. Here we used a paradigm of electrical stimulation comprisi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00060-9
更新日期:2000-01-01 00:00:00
abstract::Midbrain dopamine neurons in the ventral tegmental area, substantia nigra and retrorubral field play key roles in reward processing, learning and memory, and movement. Within these midbrain regions and admixed with the dopamine neurons, are also substantial populations of GABAergic neurons that regulate dopamine neuro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.01.046
更新日期:2008-04-09 00:00:00
abstract::Interactions between neurotransmitters and the immune system represent new prospects for understanding neuroinflammation and associated neurological disease. GABA is the chief inhibitory neurotransmitter but its actions on immune pathways in the brain are unclear. In the present study, we investigated GABAergic transp...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.04.037
更新日期:2014-07-25 00:00:00
abstract::Acetylcholinesterase is released from both axon terminals and dendrites of nigrostriatal neurons. The relationship of this phenomenon to: (1) activation of receptors, and (2) firing rate, has been examined. In the first series of experiments, apomorphine or acetylcholine were infused into substantiae nigrae of urethan...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90078-3
更新日期:1986-04-01 00:00:00
abstract::Pain is processed in a large neural network that partially overlaps structures involved in emotion processing. Despite the fact that pain and emotion are known to share neural regions and interact in numerous clinical conditions, relatively little is known about the interaction of pain and emotion at the neural level....
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.02.047
更新日期:2017-05-04 00:00:00
abstract::Intrauterine growth restriction (IUGR) associates with increased preference for palatable foods and altered insulin sensitivity. Insulin modulates the central dopaminergic response and changes behavioral responses to reward. We measured the release of dopamine in the accumbens during palatable food intake in IUGR rats...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.12.036
更新日期:2019-02-21 00:00:00
abstract::Huntington's disease (HD) is a severe genetically inherited neurodegenerative disorder. Patients present with three principal phenotypes of motor symptoms: choreatic, hypokinetic-rigid and mixed. The Q175 mouse model of disease offers an opportunity to investigate the cellular basis of the hypokinetic-rigid form of HD...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.10.039
更新日期:2015-12-17 00:00:00
abstract::Anhedonia is a core symptom of clinical depression. Two brain neuropeptides that have been implicated in anhedonia symptomology in preclinical depression models are dynorphin and orexin; which are concentrated along lateral hypothalamic dopamine reward pathways. These affect regulating neuropeptides modulate each othe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.05.033
更新日期:2012-08-30 00:00:00
abstract::Persistence of horizontal cell (HC) light responses in extracellular solutions containing low Ca2+ plus divalent cations to block Ca2+ currents (ICa) has been attributed to Ca2+-independent neurotransmission. Using a retinal slice preparation to record both ICa and light responses, we demonstrate that persistence of H...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.08.043
更新日期:2004-01-01 00:00:00
abstract::The neuromodulator adenosine maintains brain homeostasis and regulates complex behaviour via activation of inhibitory and excitatory adenosine receptors (ARs) in a brain region-specific manner. AR antagonists such as caffeine have been shown to ameliorate cognitive impairments in animal disease models but their effect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.03.051
更新日期:2012-06-28 00:00:00
abstract::A variety of voltage-gated ion channels are expressed on principal cell dendrites and have been proposed to play a pivotal role in the regulation of dendritic excitability. Previous studies at the light microscopic level demonstrated that the K+ channel subunit Kv2.1 expression was polarized to the cell soma and dendr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00519-8
更新日期:1998-05-01 00:00:00
abstract::Laminins are the major glycoproteins present in basement membrane, a type of extracellular matrix. We showed that the LAMB1 gene, which encodes the laminin beta1 subunit, is transcriptionally activated by retinoic acid in embryonic stem cells. However, little information is available concerning LAMB1 developmental reg...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.03.064
更新日期:2004-01-01 00:00:00
abstract::Cell surface glycoconjugates are thought to mediate cell-cell recognition and play roles in neuronal development and functions. We demonstrated here that exposure of neuronal cells to nanomolar levels of gangliosides Neu5Acalpha 8Neu5Acalpha 3Galbeta 4GlcCer, Galbeta 3GalNAcbeta 4(Neu5Acalpha 8Neu5Acalpha 3)Galbeta 4G...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00259-8
更新日期:2003-01-01 00:00:00
abstract::Activation of microglia is regulated by controlling both its population size (through modulation of proliferation/death) and the production of inflammatory mediators. Retinoids control cellular proliferation, differentiation, and death. Natural retinoids have been shown to exhibit anti-inflammatory actions against act...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.053
更新日期:2011-09-29 00:00:00
abstract::A major input to the substantia nigra is from the 5-hydroxytryptamine-containing neurons in the dorsal raphe nucleus. In order to examine the morphology and distribution of this projection, rats were given injections of the anterograde tracers, Phaseolus vulgaris-leucoagglutinin or biocytin, in the dorsal raphe nucleu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90510-m
更新日期:1993-07-01 00:00:00
abstract::Considerable evidence suggests that dynorphin participates in the regulation of energy balance. In this study, we have used immunohistochemistry to investigate in detail the cellular localization of pro-dynorphin (DYN) immunoreactive cell bodies in the mediobasal hypothalamus with special reference to neurons producin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.04.011
更新日期:2008-06-26 00:00:00
abstract::In the present normal anatomical light and electron microscopic study in the rat, histochemical (Nissl, Timm, Golgi) or immunocytochemical (microtubule-associated protein type 2, glutamate decarboxylase, glutamate receptor subunit 1, synaptophysin) stains were used to analyse neurons embedded within the stria terminal...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00560-5
更新日期:1998-06-01 00:00:00
abstract::Leptin and somatostatin (SRIF) have opposite effects on food seeking and ingestive behaviors, functions partially regulated by the frontoparietal cortex and hippocampus. Although it is known that the acute suppression of food intake mediated by leptin decreases with time, the counter-regulatory mechanisms remain uncle...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.019
更新日期:2013-11-12 00:00:00
abstract::We examined the effects of 3-nitropropionic acid-induced succinate dehydrogenase inhibition on neuronal ATP content, N-methyl-D-aspartate-induced neuronal death, resting membrane potential, and N-methyl-D-aspartate-induced changes in cytosolic calcium concentration ([Ca2+]c) in cultured rat striatal neurons. Exposure ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00389-8
更新日期:1998-05-01 00:00:00
abstract::Hippocampal anatomy and network organization are capable to generate drug-resistant temporal lobe epilepsy (TLE) in humans and particularly vulnerable to segmental neuronal cell loss. Surgical hippocampectomy has been proven successful in treatment and available human tissue specimens allow systematic clinico-patholog...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.08.003
更新日期:2015-11-19 00:00:00
abstract::In mice, microRNAs (miRNAs) are required for embryonic viability, and previous reports implicate miRNA participation in brain cortical neurogenesis. Here, we provide a more comprehensive analysis of miRNA involvement in cortical brain development. To accomplish this we used mice in which Dicer, the RNase III enzyme ne...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.08.009
更新日期:2012-10-25 00:00:00
abstract::This immunocytochemical study, using a double-staining method, showed that calcitonin gene-related peptide-like immunoreactive structures are widely distributed in the peripheral nervous system and that many of them coexist with substance P-like immunoreactive structures in single sensory ganglion cells. Neurons posit...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90265-9
更新日期:1985-08-01 00:00:00
abstract::Known to exert an important modulatory influence on the cerebral cortex, the cholinergic neurons of the basal forebrain are modulated in turn by neurotransmitters which may include acetylcholine released from processes of brainstem or forebrain neurons. In the present study, we examined the effect of carbachol, a non-...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00167-x
更新日期:1997-11-01 00:00:00
abstract::Hippocampal neurogenesis in adult mammals is influenced by many factors. Lesioning of the entorhinal cortex is a standard model used to study injury and repair in the hippocampus. Here we use bromodeoxyuridine (BrdU) labeling combined with immunohistochemical identification using cell type specific markers to follow t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.05.058
更新日期:2004-01-01 00:00:00
abstract::Regulation of adult hippocampal neurogenesis in mice responds to behavioral stimuli, including physical activity (RUN) and the exposure to enriched environments (ENR). If studied after days or weeks, these stimuli and the pathological stimulus of kainic acid-induced seizures (KA) show differential effects on different...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.04.023
更新日期:2008-06-23 00:00:00
abstract::Previous experiments with wheatgerm agglutinin, an inhibitor of ionotropic quisqualate receptor desensitization, suggest that desensitization regulates quisqualate receptor-mediated synaptic transmission and excitotoxicity. Using whole-cell recordings from cultured postnatal rat hippocampal neurons, we have examined t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90179-j
更新日期:1993-01-01 00:00:00
abstract::Brain microvascular endothelial cell (BMEC) injury induced by ischemia-reperfusion (I/R) is the initial stage of blood-brain barrier (BBB) disruption, which results in a poor prognosis in ischemic stroke patients. Autophagy has been shown to have protective effects on BMECs against cerebral ischemic insults. However, ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.04.017
更新日期:2017-06-23 00:00:00
abstract::Some studies have demonstrated that aging is associated with impaired vestibular reflexes, especially otolithic reflexes, resulting in postural instability. However, the neurochemical basis of these age-related changes is still poorly understood. The l-arginine metabolic system has been implicated in changes in the br...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.02.033
更新日期:2017-04-21 00:00:00