Abstract:
:Considerable evidence suggests that dynorphin participates in the regulation of energy balance. In this study, we have used immunohistochemistry to investigate in detail the cellular localization of pro-dynorphin (DYN) immunoreactive cell bodies in the mediobasal hypothalamus with special reference to neurons producing orexigenic or anorexigenic transmitters. In colchicine-treated rats, DYN immunoreactivity was demonstrated in many cell bodies of the arcuate nucleus (Arc). Double-labeling revealed that DYN immunoreactivity was present in approximately 30% of pro-opiomelanocortin (POMC) neurons in the ventrolateral Arc as shown by presence of alpha-melanocyte-stimulating hormone (alpha-MSH) and cocaine- and amphetamine-regulated transcript (CART). In contrast, DYN immunoreactivity was not demonstrated in agouti-related peptide (AgRP)- or neuropeptide Y (NPY) -containing neurons in the ventromedial aspect of the Arc. Dynorphin immunoreactivity was also colocalized with the vesicular acetylcholine (ACh) transporter (VAChT; a marker for cholinergic neurons) in the cell soma of Arc POMC neurons. Brainstem POMC neurons in the commissural part of the solitary tract nucleus (NTS) were devoid of DYN immunoreactivity, whereas DYN immunoreactivity was detected in a few NPY-containing NTS neurons and cholinergic DMX neurons. Our results showing presence of DYN together with alpha-MSH in a subpopulation of hypothalamic POMC neurons further point to the neurochemical heterogeneity of hypothalamic POMC neurons. The results suggest a role for DYN in control of energy balance by mediating the effect of peripheral hormones such as leptin and insulin.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Maolood N,Meister Bdoi
10.1016/j.neuroscience.2008.04.011subject
Has Abstractpub_date
2008-06-26 00:00:00pages
1121-31issue
3eissn
0306-4522issn
1873-7544pii
S0306-4522(08)00577-0journal_volume
154pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The distribution of axoplasmic organelles at consecutive nodes of Ranvier was investigated light- and electronmicroscopically in horseradish peroxidase-transporting muscle nerve fibres of the adult cat. Three main and apparently temporally interrelated distribution patterns were noted. These patterns were referred to ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90148-x
更新日期:1986-12-01 00:00:00
abstract::In the adult CNS, GABA is the predominant inhibitory neurotransmitter, mediating the hyperpolarization of membrane potential and regulating the glutamatergic activity. In the immature CNS, on the other hand, GABA mediates depolarization and is involved in controlling morphogenesis. This developmental shift in GABA act...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.08.044
更新日期:2006-12-01 00:00:00
abstract::The adverse consequences of early-life sleep deprivation on mental health are well recognized, yet many aspects remain unknown, therefore, animal studies can offer useful insights. Male Sprague-Dawley rats at postnatal day (PND) 19 were subjected to sleep deprivation (SD) for 14 days (6-8 hours/day). Control (CON) rat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.02.021
更新日期:2019-05-15 00:00:00
abstract::The expression pattern of the pannexin2 protein (Px2) in healthy and ischemized brains of adult rats was investigated. A polyclonal antibody for rat Px2 was generated in chicken and purified for affinity. This antibody was used to study by Western blot, Enzyme-Linked Immunosorbent Assay, and immunohistochemistry, the ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.06.028
更新日期:2007-09-07 00:00:00
abstract::Brainstem projections to hypoglossal motoneurons innervating the intrinsic and extrinsic muscles of the tongue were determined using the transneuronal transfer of Herpes simplex virus-1. Injections of Herpes simplex virus-1 into the intrinsic muscles of the anterior tongue, the geniohyoid and styloglossus muscles each...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00203-u
更新日期:1995-10-01 00:00:00
abstract::Neuronal loss is the most common and critical feature of a spectrum of brain traumas and neurodegenerative disorders such as Alzheimer's disease (AD). The capacity to generate new neurons in the central nervous system diminishes early during brain development and is restricted mainly to two brain areas in the mature b...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.11.054
更新日期:2014-02-14 00:00:00
abstract::We studied the distribution and cellular localization of Na(+)-coupled neutral amino acid transporter 2, a member of the system A family of amino acid transporters, in the rat and human cerebral cortex using immunocytochemical methods. Na(+)-coupled neutral amino acid transporter 2-positive neurons were pyramidal and ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.02.042
更新日期:2006-06-19 00:00:00
abstract::The loss of dopamine neurons combined or not with the subsequent administration of L-DOPA in patients with Parkinson's disease or in experimental models of the disease results in altered GABAergic signaling throughout the basal ganglia, including the striatum and the substantia nigra, pars reticulata. However, the mol...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.12.001
更新日期:2007-03-16 00:00:00
abstract::Localized distension of the intestine evokes an ascending excitatory reflex and a descending inhibitory reflex in the circular muscle layer. The sequential activation of these two reflexes is believed to underlie the motor pattern of peristalsis, which is responsible for the co-ordinated propulsion of intestinal conte...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00040-1
更新日期:1996-07-01 00:00:00
abstract::The sec6/8 (exocyst) complex is implicated in targeting of vesicles for regulated exocytosis in various cell types and is believed to play a role in synaptogenesis and brain development. We show that the subunits sec6 and sec8 are present at significant levels in neurons of adult rat brain, and that immunoreactivity f...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00065-4
更新日期:2003-01-01 00:00:00
abstract::The membrane properties and receptor-mediated responses of rat dorsal raphe nucleus neurons were measured using intracellular recording techniques in a slice preparation. After each experiment, the recorded neuron was filled with neurobiotin and immunohistochemically identified as 5-hydroxytryptamine (5-HT)-immunoposi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00584-5
更新日期:2003-01-01 00:00:00
abstract::In brainstem motor networks, hypoglossal motoneurons (HMs) play the physiological role of driving tongue contraction, an activity critical for inspiration, phonation, chewing and swallowing. HMs are an early target of neurodegenerative diseases like amyotrophic lateral sclerosis that, in its bulbar form, is manifested...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.10.019
更新日期:2017-12-26 00:00:00
abstract::The responses in T3-4, T10-11 and L2-3 white rami to stimulation of different zones of the bulbar ventrolateral surface were maximal when the region of about 4 mm laterally to the midline was stimulated. A weak surface stimulation of all these zones elicited only a long latency response consisting of three waves. A sh...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90236-8
更新日期:1986-01-01 00:00:00
abstract::Obesity may result from dysfunction of the reward system, especially in the nucleus accumbens (Acb). Based on this hypothesis, many researchers have tested the effect of high-frequency stimulation (HFS) of the Acb shell (Acb-Sh) and/or core (Acb-Co) on ingestive behaviors, but few studies have explored the possible me...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.006
更新日期:2015-08-20 00:00:00
abstract::Ablation of capsaicin-sensitive afferent neurons enhances experimentally induced ulceration in the rat gastric mucosa, which suggests that these neurons are involved in gastric mucosal protection. To provide direct evidence for such a function it was investigated whether stimulation of afferent nerve endings by the in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90201-1
更新日期:1988-12-01 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARγ) belongs to a family of ligand-activated transcription factors, and its ligands are known to control many physiological and pathological conditions. The hypothesis of our study was that the PPARγ agonist (rosiglitazone) could mediate tumor necrosis factor alpha (...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.11.003
更新日期:2013-01-15 00:00:00
abstract::Protein F1/GAP-43 is a protein kinase C substrate associated with axonal growth and synaptic plasticity. We used in situ hybridization in rat brain to determine the cellular distribution of its gene expression. Throughout the septotemporal axis of the adult hippocampus, pyramidal cells express F1/GAP-43 mRNA, but gran...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90284-u
更新日期:1991-01-01 00:00:00
abstract::The effect of intrathecal or intracerebroventricular administration of the GABAB receptor agonist, baclofen, on rhythmic contractions induced by distension of the urinary bladder (micturition reflex) was evaluated in urethane-anesthetized rats. Baclofen inhibited bladder motility acting at central nervous system sites...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90350-b
更新日期:1992-01-01 00:00:00
abstract::To be highly reliable, synaptic transmission needs postsynaptic receptors (Rs) in precise apposition to the presynaptic release sites. At inhibitory synapses, the postsynaptic protein gephyrin self-assembles to form a scaffold that anchors glycine and GABAARs to the cytoskeleton, thus ensuring the accurate accumulatio...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2019.07.036
更新日期:2020-07-15 00:00:00
abstract::In situ hybridization was used to measure the levels of proenkephalin mRNA and prodynorphin mRNA in regions of rat striatum and nucleus accumbens. Chronic administration of haloperidol (2.4 mg/kg/day for 7 days) increased the levels of proenkephalin mRNA in both striatum and nucleus accumbens. In contrast, the levels ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90256-4
更新日期:1988-05-01 00:00:00
abstract::Trigeminal neurogenic inflammation is one explanation for the development of vascular migraine. The triggers for this inflammation and pain are not well understood, but are probably vasoactive components acting on the blood vessel wall. Migraine-related inner ear symptoms like phonophobia, tinnitus, fluctuation in hea...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00521-2
更新日期:2001-01-01 00:00:00
abstract::Peptidergic dorsal root ganglion (DRG) neurons transmit sensory and nociceptive information from the periphery to the central nervous system. Their synaptic activity is profoundly affected by neuromodulatory peptides stored and released from large dense-core vesicles (LDCVs). However, the mechanism of peptide secretio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.01.006
更新日期:2017-03-27 00:00:00
abstract::Microtubule-associated protein 1B is a major constituent of the neuronal cytoskeleton during the early stages of development. This protein and its phosphorylated isoform, microtubule-associated protein 1B-P, defined by the monoclonal antibody 1B-P [Boyne L. J. et al. (1995) J. Neurosci. Res. 40, 439-450], are present ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00141-x
更新日期:2000-01-01 00:00:00
abstract::Cocaine- and amphetamine-regulated transcript (CART) and nesfatin-1/nucleobindin 2 (NUCB2) are assumed to play a role in feeding and adaptation to stress. Both peptides are highly expressed in the midbrain non-preganglionic Edinger-Westphal nucleus (npEW), a center implicated in the regulation of stress adaptation and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.07.001
更新日期:2010-10-13 00:00:00
abstract::The roles of acetylcholine and dynorphin (1-13) in the presynaptic control of the release of [3H]dopamine continuously synthesized from [3H]tyrosine were examined in a prominent striosomal enriched area and in an adjacent matrix enriched area of the cat caudate nucleus. This was achieved using microsuperfusion devices...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90340-t
更新日期:1991-01-01 00:00:00
abstract::A delayed-matching spatial working memory protocol in a 5-arm maze was used to test the hypothesis of differential roles for central nicotinic and muscarinic cholinergic receptors in mediating task performance. In experiment 1, using a within subjects-repeated design, groups of C57Bl/6 mice, previously trained to crit...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00403-2
更新日期:2003-01-01 00:00:00
abstract::The roles of the central noradrenergic and serotonergic system in the activity-dependent regulation of ocular dominance plasticity have been a contentious issue. Using c-Fos activity mapping, we have developed a new, straightforward method to measure the strength of ocular dominance plasticity: the number of c-Fos-imm...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.01.013
更新日期:2013-04-03 00:00:00
abstract::Astrocyte hypertrophy and swelling occur in a variety of pathophysiological conditions, including diseases associated with hyperammonemia. Ammonia is rapidly incorporated into glutamine by glutamine synthetase localized in astrocytes. We tested the hypotheses that (1) 6 h of hyperammonemia (500-600 microM) is adequate...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00462-9
更新日期:1996-03-01 00:00:00
abstract::Orientation tuning of 271 neurons of the cat visual cortex (area 17) was studied with a light bar flashing in the receptive field. Under different conditions, 27-57% of units were found to have double-orientation tuning: they demonstrated the main preferred orientation and an additional preferred orientation. The stat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90416-2
更新日期:1994-08-01 00:00:00
abstract::The present study set out to assess the possible role of the medial prefrontal cortex (mPFC) cannabinoid CB1 receptors and BDNF/cFOS signaling pathways in morphine-dextromethorphan (DXM) cross state-dependent memory (SDM) using male Wistar rats. Changes on the levels of BDNF and cFOS proteins in the PFC were examined ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.10.012
更新日期:2018-11-21 00:00:00