Enzymatic assay for cholesterol ester hydrolase activity.

abstract：:Major neutral glycosphingolipid components were extracted from Sporothrix schenckii, a dimorphic fungus exhibiting a hyphal saprophytic phase and a yeast parasitic phase responsible for chronic mycotic infections in mammalian hosts. These components, one from the mycelial form and two from the yeast form, were purifie...

journal_title：Journal of lipid research

authors： Toledo MS,Levery SB,Straus AH,Takahashi HK

更新日期：2000-05-01 00:00:00

abstract：:Conventional ideas concerning the unidirectional movement of triacylglycerol from intestinal lumen to lymph with sn-2-monoacylglycerol being the major glyceride-glycerol precursor were challenged by our finding that steady state specific activities of radiolabeled triacylglycerol (glyceryl moiety) in the intestinal mu...

journal_title：Journal of lipid research

authors： Mansbach CM 2nd,Parthasarathy S

更新日期：1982-09-01 00:00:00

abstract：:A gas-liquid chromatography-flame ionization detection method is described for measuring plasma levels of eicosatetraynoic acid using eicosatriynoic acid as an internal standard. The technique is simple, rapid, and reproducible. Eicosatetraynoic acid and eicosatriynoic acid behave similarly in the extraction system ch...

journal_title：Journal of lipid research

authors： Goodman RP,Brash AR

更新日期：1981-03-01 00:00:00

abstract：:Supersaturation and rapid nucleation of cholesterol in bile are of key importance in the pathogenesis of cholesterol gallstones. While the effects of bile acids and phospholipids on cholesterol saturation of bile have been extensively studied, their influence on the cholesterol nucleation time has not been compared. W...

journal_title：Journal of lipid research

authors： Jüngst D,Lang T,Huber P,Lange V,Paumgartner G

更新日期：1993-09-01 00:00:00

abstract：:Hepatic steatosis is one of the most common liver disorders in the general population. The main cause of hepatic steatosis is nonalcoholic fatty liver disease (NAFLD), representing the hepatic component of the metabolic syndrome, which is characterized by type 2 diabetes, obesity, and dyslipidemia. Insulin resistance ...

journal_title：Journal of lipid research

authors： Hooper AJ,Adams LA,Burnett JR

更新日期：2011-04-01 00:00:00

abstract：:Apolipoprotein A-IV (apoA-IV) has been postulated to be antiatherogenic. Transgenic APOA4/Apoe-/- mice are protected against atherosclerosis, with plasma apoA-IV displaying antioxidant activity in vitro. In humans, there is an inverse relationship between apoA-IV levels and risk of coronary heart disease (CHD). Furthe...

journal_title：Journal of lipid research

authors： Wong WM,Stephens JW,Acharya J,Hurel SJ,Humphries SE,Talmud PJ

更新日期：2004-08-01 00:00:00

abstract：:Cholelithiasis is a multifactorial process, and several mechanisms have been postulated. A decreased expression of the ileal apical sodium-dependent bile acid transporter (ASBT) and of the cytosolic ileal lipid binding protein (ILBP) was recently described in female non-obese patients. The role of the recently identif...

journal_title：Journal of lipid research

authors： Renner O,Harsch S,Strohmeyer A,Schimmel S,Stange EF

更新日期：2008-09-01 00:00:00

abstract：:Lipid metabolism plays an important role in the regulation of cellular homeostasis. However, because it is difficult to measure the actual rates of synthesis and degradation of individual lipid species, lipid compositions are often used as a surrogate to evaluate lipid metabolism even though they provide only static s...

journal_title：Journal of lipid research

authors： Schlame M,Xu Y,Erdjument-Bromage H,Neubert TA,Ren M

更新日期：2020-01-01 00:00:00

abstract：:Sixteen hyperlipidemic men were enrolled in a randomized, placebo-controlled, double-blind, cross-over study to evaluate the effect of ezetimibe 10 mg and simvastatin 40 mg, coadministered and alone, on the in vivo kinetics of apolipoprotein (apo) B-48 and B-100 in humans. Subjects underwent a primed-constant infusion...

journal_title：Journal of lipid research

authors： Tremblay AJ,Lamarche B,Hogue JC,Couture P

更新日期：2009-07-01 00:00:00

abstract：:The inverse relationship between plasma levels of high density lipoprotein (HDL) and coronary heart disease rates has suggested that HDL might influence body stores of cholesterol. Therefore, we have investigated potential relationships between the parameters of body cholesterol metabolism and the plasma levels of HDL...

journal_title：Journal of lipid research

authors： Blum CB,Dell RB,Palmer RH,Ramakrishnan R,Seplowitz AH,Goodman DS

更新日期：1985-09-01 00:00:00

abstract：:Adiponectin, an adipocyte-derived protein, has anti-atherogenic and anti-diabetic effects, but how it confers the anti-atherogenic effects is not well understood. To study the anti-atherogenic mechanisms of adiponectin, we examined whether it interacts with atherogenic low-density lipoprotein (LDL) to attenuate LDL's ...

journal_title：Journal of lipid research

authors： Kakino A,Fujita Y,Ke LY,Chan HC,Tsai MH,Dai CY,Chen CH,Sawamura T

更新日期：2020-11-03 00:00:00

abstract：:Expression of bile salt export pump (BSEP) is regulated by the bile acid/farnesoid X receptor (FXR) signaling pathway. Two FXR isoforms, FXRα1 and FXRα2, are predominantly expressed in human liver. We previously showed that human BSEP was isoform-dependently regulated by FXR and diminished with altered expression of F...

journal_title：Journal of lipid research

authors： Song X,Chen Y,Valanejad L,Kaimal R,Yan B,Stoner M,Deng R

更新日期：2013-11-01 00:00:00

abstract：:The pregnane X receptor (PXR, also known as SXR) is a nuclear hormone receptor activated by xenobiotics as well as diverse sterols and their metabolites. PXR functions as a xenobiotic sensor to coordinately regulate xenobiotic metabolism via transcriptional regulation of xenobiotic-detoxifying enzymes and transporters...

journal_title：Journal of lipid research

authors： Sui Y,Xu J,Rios-Pilier J,Zhou C

更新日期：2011-09-01 00:00:00

abstract：:Gangliosides and sulfatides (STs) are acidic glycosphingolipids (GSLs) that have one or more sialic acids or sulfate substituents, in addition to neutral sugars, attached to the C-1 hydroxyl group of the ceramide long chain base. TLC is a widely employed and convenient technique for separation and characterization of ...

journal_title：Journal of lipid research

authors： Park H,Zhou Y,Costello CE

更新日期：2014-04-01 00:00:00

abstract：:Although numerous studies have investigated the relationship between cholesteryl ester transfer protein (CETP) and high density lipoprotein (HDL) remodeling, the relationship between CETP and low density lipoproteins (LDL) is still not fully understood. In the present study, we examined the effect of the inhibition of...

journal_title：Journal of lipid research

authors： Sugano M,Sawada S,Tsuchida K,Makino N,Kamada M

更新日期：2000-01-01 00:00:00

abstract：:A preparation of cerebral microvessels was used to demonstrate the presence of lipoprotein lipase and acid lipase activity in the microvasculature of rabbit brain. Microvessels, consisting predominantly of capillaries, small arterioles, and venules, were islated from rabbit brain. Homogenates were assayed for lipolyti...

journal_title：Journal of lipid research

authors： Brecher P,Kuan HT

更新日期：1979-05-01 00:00:00

abstract：:Adipocyte differentiation and function have become areas of intense focus in the field of energy metabolism; however, understanding the role of specific genes in the establishment and maintenance of fat cell function can be challenging and complex. In this review, we offer practical guidelines for the study of adipocy...

journal_title：Journal of lipid research

authors： Wang QA,Scherer PE,Gupta RK

更新日期：2014-04-01 00:00:00

abstract：:The present study was undertaken to investigate the potentiation by p-chlorophenoxyisobutyrate (CPIB) of the antilipolytic effect of insulin in isolated adipocytes from rats fed a (1) sucrose diet, (2) glycerol-lard diet, or (3) chow diet. CPIB supplementation in the diet consistently resulted in decreased serum trigl...

journal_title：Journal of lipid research

authors： Ip C,Tepperman HM,Tepperman J

更新日期：1976-01-01 00:00:00

abstract：:Low concentrations of oxidized low density lipoprotein (OxLDL) are cytoprotective for phagocytes, although the underlying mechanisms remain unclear. We investigated signaling pathways used by OxLDL to attenuate apoptosis in monocytic cells. OxLDL at 25-50 mug/ml inhibited staurosporine-induced apoptosis in THP-1 cells...

journal_title：Journal of lipid research

authors： Namgaladze D,Kollas A,Brüne B

更新日期：2008-01-01 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) induces the translocation of newly synthesized cholesterol as well as caveolin-1 to the cytosolic lipid-protein particle (CLPP) fraction in astrocytes before its appearance in high density lipoprotein generated in the medium (Ito, J., Y. Nagayasu, K. Kato, R. Sato, and S. Yokoyama. 2002. Ap...

journal_title：Journal of lipid research

authors： Ito J,Li H,Nagayasu Y,Kheirollah A,Yokoyama S

更新日期：2004-12-01 00:00:00

abstract：:Extracted tissue phospholipid 31P NMR profiles, obtained from individual porcine lenses subjected to two preservation procedures (acetone desiccation and freeze-drying) and a perchloric acid-extraction procedure, were compared to those from freshly excised lens specimens. Each profile yielded quantitative data on 12 l...

journal_title：Journal of lipid research

authors： Meneses P,Para PF,Glonek T

更新日期：1989-03-01 00:00:00

abstract：:The extent and site(s) of inhibition of cholesterol absorption by plant sterols, sitosterol and fucosterol, were studied in rats. The intragastric administration of a single emulsified lipid meal containing 25 mg [3H]cholesterol and 25 mg of either sitosterol or fucosterol inhibited the lymphatic absorption of cholest...

journal_title：Journal of lipid research

authors： Ikeda I,Tanaka K,Sugano M,Vahouny GV,Gallo LL

更新日期：1988-12-01 00:00:00

abstract：:Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs). The premise of gene therapy for this group of diseases is borne of findings that genetic...

journal_title：Journal of lipid research

authors： Cheng SH

更新日期：2014-09-01 00:00:00

abstract：:Long-chain acyl-CoA synthetases (ACSLs) catalyze the thioesterification of long-chain FAs into their acyl-CoA derivatives. Purified ACSL4 is an arachidonic acid (20:4)-preferring ACSL isoform, and ACSL4 is therefore a probable regulator of lipid mediator production in intact cells. Eicosanoids play important roles in ...

journal_title：Journal of lipid research

authors： Golej DL,Askari B,Kramer F,Barnhart S,Vivekanandan-Giri A,Pennathur S,Bornfeldt KE

更新日期：2011-04-01 00:00:00

abstract：:A group of polyunsaturated fatty acids called conjugated linoleic acids (CLAs) are found in ruminant products, where the most common isomers are cis9, trans11 (c 9,t11) and trans10, cis12 (t10,c12) CLA. A crude mixture of these isomers has been shown in animal studies to alter body composition by a reduction in body f...

journal_title：Journal of lipid research

authors： Granlund L,Juvet LK,Pedersen JI,Nebb HI

更新日期：2003-08-01 00:00:00

abstract：:Thyroid hormone lowers serum cholesterol and alters sterol metabolic processes. This laboratory has previously reported increased biliary lipid secretion as an early effect of triiodothyronine (T3) in the rat. To evaluate whether the bile lipid action of T3 is a primary or secondary effect, the isolated-perfused rat l...

journal_title：Journal of lipid research

authors： Gebhard RL,Stone BG,Andreini JP,Duane WC,Evans CD,Prigge W

更新日期：1992-10-01 00:00:00

abstract：:ATP-citrate lyase (ACL) is a key lipogenic enzyme that converts citrate in the cytoplasm to acetyl-CoA, the initial precursor that yields malonyl-CoA for fatty acid biosynthesis. As cytosolic citrate is derived from the tricarboxylic acid cycle in the mitochondrion, ACL catalyzes a critical reaction linking cellular g...

journal_title：Journal of lipid research

authors： Wang Q,Li S,Jiang L,Zhou Y,Li Z,Shao M,Li W,Liu Y

更新日期：2010-09-01 00:00:00

abstract：:The methanolyzed lipids of the extreme halophile, Halobacterium cutirubrum, were separated into glycerol diether and glycerol monoether fractions. The diether was shown by synthesis to be 2,3-di-O-(3'R,7'R,11'R,15'-tetramethylhexadecyl)-sn-glycerol. The monoether fraction was separated by thin-layer chromatography on ...

journal_title：Journal of lipid research

authors： Joo CN,Shier T,Kates M

更新日期：1968-11-01 00:00:00

abstract：:Thermal and lyotropic phase behavior was studied by X-ray diffraction and differential scanning calorimetry for a diether phosphonolipid analog (DEPN-8) of the major lung surfactant glycerophospholipid dipalmitoyl phosphatidylcholine (DPPC). DEPN-8 differs in an ether, rather than an ester, bond at the acyl chain-back...

journal_title：Journal of lipid research

authors： Skita V,Chester DW,Oliver CJ,Turcotte JG,Notter RH

更新日期：1995-05-01 00:00:00

abstract：:3 alpha,7 alpha-dihydroxy-5 beta-cholestan-26-oic acid (DHCA) and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA) are metabolized into chenodeoxycholic acid and cholic acid, respectively, through oxidation and cleavage of the terminal three carbons of the side chain. The present study was desig...

journal_title：Journal of lipid research

authors： Cass OW,Williams GC,Hanson RF

更新日期：1980-02-01 00:00:00