Abstract:
:Mitochondria, isolated from the ubiquinone-deficient nuclear mutant of Saccharomyces cerevisiae E3-24, are practically unable to oxidize exogenous substrates. Respiratory activity, coupled to ATP synthesis, can, however, be reconstituted by the simple addition of ethanolic solutions of ubiquinones. A minimal length of the isoprenoid side chain (greater than or equal to 3) was required for the restoration. Saturation of the reconstitution required a large amount of exogeneous ubiquinone, in excess over the normal content present in the mitochondria of the wild type strain. A similar pattern of reconstituted activities could be also obtained using sonicated inverted particles. Mitochondria and sonicated particles are also able to carry out a dye-mediated electron flow coupled to ATP synthesis in the absence of added ubiquinone, using ascorbate or succinate as electron donor. This demonstrates that the energy conserving mechanism at the third coupling site of the respiratory chain is fully independent of the presence of the large mobile pool of ubiquinone in the membrane.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
De Santis A,Bertoli E,Di Gioia A,Melandri BA,Baccarini Melandri Adoi
10.1007/BF00745017subject
Has Abstractpub_date
1982-06-01 00:00:00pages
159-69issue
3eissn
0145-479Xissn
1573-6881journal_volume
14pub_type
杂志文章abstract::Ferrochelatase [heme synthase, protoheme ferrolyase (EC 4.99.1.1)], the terminal enzyme of the heme biosynthetic pathway, catalyzes the incorporation of ferrous ion into protoporphyrin IX to form protoheme IX. The genes and cDNAs for ferrochelatase from mammals and micro-organisms have been isolated. The gene for huma...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110038
更新日期:1995-04-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00744524
更新日期:1983-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:1991-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00745047
更新日期:1983-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:1992-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-016-9643-2
更新日期:2016-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00745010
更新日期:1980-04-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00744687
更新日期:1980-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1020595108560
更新日期:1998-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00750283
更新日期:1976-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-010-9268-9
更新日期:2010-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-011-9377-0
更新日期:2011-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00771014
更新日期:1991-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/B:JOBB.0000041776.31885.45
更新日期:2004-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1005488105076
更新日期:1999-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762352
更新日期:1992-12-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-011-9340-0
更新日期:2011-04-01 00:00:00
abstract::The multi-subunit vacuolar-type H(+)-ATPase consists of a V(1) domain (A-H subunits) catalyzing ATP hydrolysis and a V(0) domain (a, c, c', c", d, e) responsible for H(+) translocation. The mammalian V(0) d subunit is one of the least-well characterized, and its function and position within the pump are still unclear....
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-008-9161-y
更新日期:2008-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1005580020547
更新日期:2000-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type:
doi:10.1007/s10863-012-9470-z
更新日期:2012-12-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9441-4
更新日期:2012-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00769536
更新日期:1992-02-01 00:00:00
abstract::In this review, I focus on the bioenergetics of the methanogenic bacteria, with particular attention directed to the roles of transmembrane electrochemical gradients of sodium and proton. In addition, the mechanism of coupling ATP synthesis to methanogenic electron transfer is addressed. Evidence is reviewed which sug...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762689
更新日期:1989-12-01 00:00:00
abstract::Protein phosphorylation is an important mechanism in the modulation of voltage-dependent ionic channels. In squid giant axons, the potassium delayed rectifier channel is modulated by an ATP-mediated phosphorylation mechanism, producing important changes in amplitude and kinetics of the outward current. The characteris...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00785813
更新日期:1991-08-01 00:00:00