Thyrotropin-releasing hormone mobilizes Ca2+ from endoplasmic reticulum and mitochondria of GH3 pituitary cells: characterization of cellular Ca2+ pools by a method based on digitonin permeabilization.

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:Despite the increasing number of regulatory proteins identified in clathrin-independent endocytic (CIE) pathways, our understanding of the exact functions of these proteins and the sequential manner in which they function remains limited. In this study, using the Caenorhabditis elegans intestine as a model, we observe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen S,Li L,Li J,Liu B,Zhu X,Zheng L,Zhang R,Xu T

更新日期：2014-10-28 00:00:00

abstract：:Multiplex assays, involving the simultaneous use of multiple circulating tumor DNA (ctDNA) markers, can improve the performance of liquid biopsies so that they are highly predictive of cancer recurrence. We have developed a single-tube methylation-specific quantitative PCR assay (mqMSP) that uses 10 different methylat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin S,Zhu D,Shao F,Chen S,Guo Y,Li K,Wang Y,Ding R,Gao L,Ma W,Lu T,Li D,Zhang Z,Cai S,Liang X,Song H,Ji L,Li J,Zheng Z,Jiang F,Wu X,Luan J,Zhang H,Yang Z,Cantor CR,Xu C,Ding C

更新日期：2021-02-02 00:00:00

abstract：:Therapeutics for cerebral ischemia/hypoxia, which often results in ischemic stroke in humans, are a global unmet medical need. Here, we report that bryostatin-1, a highly potent protein kinase C (PKC) activator, interrupts pathophysiological molecular cascades and apoptosis triggered by cerebral ischemia/hypoxia, enha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun MK,Hongpaisan J,Alkon DL

更新日期：2009-08-25 00:00:00

abstract：:Factors that elevate amyloid-beta (Abeta) peptide levels are associated with an increased risk for Alzheimer's disease. Insulysin has been identified as one of several proteases potentially involved in Abeta degradation based on its hydrolysis of Abeta peptides in vitro. In this study, in vivo levels of brain Abeta40 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller BC,Eckman EA,Sambamurti K,Dobbs N,Chow KM,Eckman CB,Hersh LB,Thiele DL

更新日期：2003-05-13 00:00:00

abstract：:The biosynthetic origin of the 10,000 molecular weight neurophysins, carriers of the peptide hormones oxytocin and vasopressin, has been studied by cell-free synthesis, Poly(A)-RNA was isolated from bovine hypothalamus and translated in a wheat germ system containing (35)S- or (3)H-labeled amino acids. A number of uni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giudice LC,Chaiken IM

更新日期：1979-08-01 00:00:00

abstract：:It is well documented that human modification of the hydrological cycle has profoundly affected the flow of liquid water across the Earth's land surface. Alteration of water vapor flows through land-use changes has received comparatively less attention, despite compelling evidence that such alteration can influence th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon LJ,Steffen W,Jönsson BF,Folke C,Falkenmark M,Johannessen A

更新日期：2005-05-24 00:00:00

abstract：:Siphoviridae is the most abundant viral family on earth which infects bacteria as well as archaea. All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment. Here, we report analysis of the p2 phage baseplate structure by X-ray cry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sciara G,Bebeacua C,Bron P,Tremblay D,Ortiz-Lombardia M,Lichière J,van Heel M,Campanacci V,Moineau S,Cambillau C

更新日期：2010-04-13 00:00:00

abstract：:Expression of the homeobox protein CDX1 is lost or reduced in a significant proportion of colorectal carcinomas (CRCs) but the underlying mechanism for this is unclear. We have demonstrated absence of CDX1 mRNA expression in 7 of 37 CRC cell lines and shown that all 7 cell lines have a methylated CDX1 promoter. Twenty...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong NA,Britton MP,Choi GS,Stanton TK,Bicknell DC,Wilding JL,Bodmer WF

更新日期：2004-01-13 00:00:00

abstract：:To identify genes important for hair-cell function, we conducted a mutagenic screen in zebrafish. Larvae from one mutant line, ru848, were unresponsive to acoustic stimuli and unable to balance. The mutation results in a 90% reduction in hair-cell number and partial retinal degeneration by 5 days postfertilization. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Starr CJ,Kappler JA,Chan DK,Kollmar R,Hudspeth AJ

更新日期：2004-02-24 00:00:00

abstract：:Rat liver membrane adenylate cyclase (EC 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. Choleragen similarly increases the sensitivity of cyclase to other peptide (ACTH, vasoactive intestinal polypeptide) and nonpeptide (ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett V,O'Keefe E,Cuatrecasaş P

更新日期：1975-01-01 00:00:00

abstract：:Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Campanacci V,Lartigue A,Hällberg BM,Jones TA,Giudici-Orticoni MT,Tegoni M,Cambillau C

更新日期：2003-04-29 00:00:00

abstract：:Respiratory cytochrome c has been found to be phosphorylated at tyrosine 97 in the postischemic brain upon neuroprotective insulin treatment, but how such posttranslational modification affects mitochondrial metabolism is unclear. Here, we report the structural features and functional behavior of a phosphomimetic cyto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guerra-Castellano A,Díaz-Quintana A,Pérez-Mejías G,Elena-Real CA,González-Arzola K,García-Mauriño SM,De la Rosa MA,Díaz-Moreno I

更新日期：2018-07-31 00:00:00

abstract：:A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit glo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aviv H,Leder P

更新日期：1972-06-01 00:00:00

abstract：:Management of the diverse fisheries of the world has had mixed success. While managing single species in data-rich environments has been largely effective, perhaps the greatest challenge facing fishery managers is how to deal with mixed stocks of fish with a range of life histories that reside in the same location. Be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hastings A,Gaines SD,Costello C

更新日期：2017-08-22 00:00:00

abstract：:BHK cells were inoculated sparsely on one face ("sparse- or s-face") of a thin glass film whose opposite face was covered with a 2- to 3-day-old confluent layer of BHK cells ("confluent- or c-face"). After 7 hr of attaching and spreading in the absence of visible light, most of the cells on the s-face traversed with t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albrecht-Buehler G

更新日期：1992-09-01 00:00:00

abstract：:Phytosulfokine-alpha [PSK-alpha, Tyr(SO(3)H)-Ile-Tyr(SO(3)H)-Thr-Gln], a sulfated mitogenic peptide found in plants, strongly promotes proliferation of plant cells in culture at very low concentrations. Oryza sativa PSK (OsPSK) cDNA encoding a PSK-alpha precursor has been isolated. The cDNA is 725 base pairs long, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang H,Matsubayashi Y,Nakamura K,Sakagami Y

更新日期：1999-11-09 00:00:00

abstract：:A sensitive assay using biotinylated ubiquitin revealed extensive ubiquitination of the large subunit of RNA polymerase II during incubations of transcription reactions in vitro. Phosphorylation of the repetitive carboxyl-terminal domain of the large subunit was a signal for ubiquitination. Specific inhibitors of cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsui A,Sharp PA

更新日期：1999-05-25 00:00:00

abstract：:Inherited deficiencies of the enzymes adenosine deaminase (adenosine aminohydrolase; EC 3.5.4.4) and purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase; EC 2.4.2.1) preferentially interfere with lymphocyte development while sparing most other organ systems. Previous experiments have s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carson DA,Kaye J,Seegmiller JE

更新日期：1977-12-01 00:00:00

abstract：:A strong mutator effect has been observed in Escherichia coli K-12 strains mutated in the bglY gene (27 min). The frequency of point mutations is not modified in bglY mutant strains. In contrast, a strong increase in spontaneous generation of large deletions has been observed in these strains, both for chromosomal mar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lejeune P,Danchin A

更新日期：1990-01-01 00:00:00

abstract：:New neurons are continuously generated in restricted regions of the adult mammalian brain. Although these adult-born neurons have been shown to receive synaptic inputs, little is known about their synaptic outputs. Using retrovirus-mediated birth-dating and labeling in combination with serial section electron microsco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faulkner RL,Jang MH,Liu XB,Duan X,Sailor KA,Kim JY,Ge S,Jones EG,Ming GL,Song H,Cheng HJ

更新日期：2008-09-16 00:00:00

abstract：:Prions are infectious agents which cause rapidly lethal neurodegenerative diseases in humans and animals following long, clinically silent incubation periods. They are composed of multichain assemblies of misfolded cellular prion protein. While it has long been assumed that prions are themselves neurotoxic, recent dev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benilova I,Reilly M,Terry C,Wenborn A,Schmidt C,Marinho AT,Risse E,Al-Doujaily H,Wiggins De Oliveira M,Sandberg MK,Wadsworth JDF,Jat PS,Collinge J

更新日期：2020-09-22 00:00:00

abstract：:The size of an organism reflects its metabolic rate, growth rate, mortality, and other important characteristics; therefore, the distribution of body size is a major determinant of ecosystem structure and function. Body-size distributions often are multimodal, with several peaks of abundant sizes, and previous studies...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lampert A,Tlusty T

更新日期：2013-01-02 00:00:00

abstract：:A light form of DNA ligase (EC 6.5.1.2), the only form present in oocytes of the axolotl (Ambystoma mexicanum), has been shown to be replaced by a heavy form of the enzyme when the egg enters cleavage. This early biochemical event has been assumed to rely on direct nuclear input. Sucrose gradient analysis permits disc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Signoret J,David JC,Lefresne J,Houillon C

更新日期：1983-06-01 00:00:00

abstract：:Although Hedgehog (Hh) signaling regulates cell differentiation during pancreas organogenesis, the consequences of pathway up-regulation in adult β-cells in vivo have not been investigated. Here, we elevate Hh signaling in β-cells by expressing an active version of the GLI2 transcription factor, a mediator of the Hh p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Landsman L,Parent A,Hebrok M

更新日期：2011-10-11 00:00:00

abstract：:Most higher plants develop severe toxicity symptoms when grown on ammonium (NH(4)(+)) as the sole nitrogen source. Recently, NH(4)(+) toxicity has been implicated as a cause of forest decline and even species extinction. Although mechanisms underlying NH(4)(+) toxicity have been extensively sought, the primary events ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Britto DT,Siddiqi MY,Glass AD,Kronzucker HJ

更新日期：2001-03-27 00:00:00

abstract：:The expression of the human HSP70 gene is induced by a wide range of physiological stresses, including exposure to heat shock and heavy metals, or under nonstress conditions, such as in response to serum stimulation. We have previously demonstrated that in either case the regulated expression is at the primary level o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu BJ,Kingston RE,Morimoto RI

更新日期：1986-02-01 00:00:00

abstract：:Equity is of fundamental concern in the quest for international cooperation to stabilize greenhouse gas concentrations by the reduction of emissions. By modeling the carbon cycle, we estimate the global CO(2) emissions that would be required to stabilize the atmospheric concentration of CO(2) at levels ranging from 45...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolin B,Kheshgi HS

更新日期：2001-04-24 00:00:00

abstract：:Murine suppressor T-cell hybridoma cells (231F1) secrete not only bioactive glycosylation-inhibiting factor (GIF) but also an inactive peptide comparable to bioactive GIF peptide in its molecular size and reactivity with anti-GIF; the amino acid sequence of the inactive peptide is identical to that of the bioactive ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano T,Liu YC,Mikayama T,Watarai H,Taniguchi M,Ishizaka K

更新日期：1995-09-26 00:00:00

abstract：:The product(s) of the Myxococcus xanthus spoC locus is required for two multicellular activities in fruiting body development, rippling and sporulation. Ripples, which are formed early in development, are spatially separated ridges of cells that move synchronously. Myxospores are heat-resistant resting cells that are ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimkets LJ,Gill RE,Kaiser D

更新日期：1983-03-01 00:00:00