Abstract：
:Although aqueous outflow is most likely a passive, nonenergy-dependent process, alterations in cellular function in the trabecular meshwork presumably are involved in the development of some types of glaucoma. Accordingly, it seems important to define both the normal and abnormal biochemistry of this tissue. The authors have chosen glycolysis as their starting point, concentrating on the regulatory enzymes, hexokinase, and, in a companion paper, phosphofructokinase. Hexokinase activity has been measured in the 100,000 X g supernatant of homogenates prepared from excised calf trabecular meshwork. Treatment of the homogenate with Triton X-100 before centrifuging caused a twofold increase in measurable activity. Electrophoresis on cellulose acetate revealed types I and II isoenzymes. Electrophoresis on starch gel further resolved type 1 into the adult and fetal subtypes. The principal isoenzyme type released into solution by Triton X-100 was type 1. The kinetic behavior of hexokinase was measured by varying the concentrations of glucose at saturating levels of ATP. Kms calculated from these plots were 7.15 X 10(-2) M, 1.78 X 10(-3) M, and 1.19 X 10(-4) M. Apart from the fetal form of type I hexokinase, the isoenzymes from trabecular meshwork resemble those of other ocular tissues and most extraocular tissues. The special role, if any, of the fetal isoenzyme in regulating glycolysis is not known. Possibly, it is a "kinetically adaptable" isoenzyme.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Anderson PJ,Karageuzian LN,Cheng HM,Epstein DLsubject
Has Abstractpub_date
1984-11-01 00:00:00pages
1258-61issue
11eissn
0146-0404issn
1552-5783journal_volume
25pub_type
杂志文章abstract：:Cell membranes from the human lens contain two major polypeptides with molecular weights near 26,000 and 22,000. These two species have been isolated in a highly purified state and purified by means of an antiserum against the 26,000 dalton membrane polypeptide from bovine lens. Immunochemical data are presented that ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：PURPOSE:The purpose of this study was to determine whether messenger RNA coding for hepatocyte growth factor (HGF), HGF receptor (MET), keratinocyte growth factor (KGF), KGF receptor, and fibroblast growth factor (FGF) receptor-2 were produced in primary cultures of human corneal epithelial, stromal fibroblast, and end...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：PURPOSE:To describe an in vivo mouse model for the study of recurrent corneal erosion syndrome (RCES) in mice and to characterize the changes in alpha9 integrin expression during wound healing. METHODS:Corneal epithelial debridement wounds of two sizes (1.5 and 2.5 mm) were made on the ocular surface of BALB/c mice an...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.03-1194
更新日期：2004-06-01 00:00:00
abstract：PURPOSE:To determine whether nonglaucomatous optic disc cupping in children violates the ISNT rule (which states that for normal optic discs the neuroretinal rim width is greatest in the order inferior >or= superior >or= nasal >or= temporal). METHODS:Digital ocular fundus photographs from a random cohort of children w...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-3837
更新日期：2010-02-01 00:00:00
abstract：Purposes:Trabeculectomy is a surgical procedure for lowering intraocular pressure in glaucoma patients, in which excessive scarring leading to failure of the filtering bleb adversely affects the surgical outcome. Heightened Tenon's capsule fibroblast (TCF) proliferation and extracellular matrix (ECM) deposition are imp...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.16-19480
更新日期：2016-10-01 00:00:00
abstract：Purpose:The trabecular meshwork (TM) has an important role in the regulation of aqueous humor outflow and IOP. Regulation of the extracellular matrix (ECM) by TGFβ2 has been studied extensively. Bone morphogenetic protein (BMP) and activin membrane-bound inhibitor (BAMBI) has been shown to inhibit or modulate TGFβ2 sig...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-23282
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:Retinal nerve fiber (RNFL) thickness and visual field loss data from patients with glaucoma were analyzed in the context of a model, to better understand individual variation in structure versus function. METHODS:Optical coherence tomography (OCT) RNFL thickness and standard automated perimetry (SAP) visual fi...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.08-2697
更新日期：2009-09-01 00:00:00
abstract：PURPOSE:Dry eye disease (DED) is associated with ocular surface inflammation that is thought to be mediated primarily by CD4 T cells. The purpose of this study was to investigate whether this T cell-mediated immune response is generated in the lymphoid compartment and to characterize the functional phenotype of the T c...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.08-2417
更新日期：2009-08-01 00:00:00
abstract：:Cultured porcine retinal pigment epithelial cells release superoxide, measured as superoxide dismutase (SOD)-suppressible reduction of cytochrome C, and SOD-suppressible luminol-dependent chemiluminescence. Latex beads stimulated a significant release of superoxide that reached 82 nmol/mg protein in the first 15 min a...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：:A simple clinical method for measurement of light backscatter from defined regions of the lens in vivo is described. Light scattering was quantitated in 168 lenses of 85 "normal" subjects spanning 8 decades of life. The results indicate that light scatter from the anterior capsule does not change with age. The light s...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：PURPOSE:To identify the differently expressed micro (mi) RNAs in pterygium compared with normal conjunctiva and investigate the potential role of miRNAs in the pathogenesis of pterygium. METHODS:With microRNA microarray and quantitative RT-PCR, we identified that microRNA-122 (miR-122) was significantly decreased in p...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.16-19402
更新日期：2016-07-01 00:00:00
abstract：PURPOSE:To measure high-resolution eye-specific displacements and deformations induced within the human LC microstructure by an acute increase in IOP. METHODS:Six eyes from donors aged 23 to 82 were scanned using second harmonic-generated (SHG) imaging at various levels of IOP from 10 to 50 mm Hg. An image registratio...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.13-12724
更新日期：2014-01-02 00:00:00
abstract：Purpose:Keratoconus (KTCN) is a complex eye disorder resulting in loss of visual function. Its development is affected by genetic and environmental components. The aim of this study was to unravel the role of epigenetic factors in KTCN. Methods:To verify if DNA methylation may play a role in KTCN development, reduced ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.18-25916
更新日期：2019-04-01 00:00:00
abstract：PURPOSE:Peroxynitrite generated during the early phase of experimental autoimmune uveoretinitis (EAU) causes peroxidation of docosahexaenoic acid (22:6), a principal unsaturated fatty acid of the photoreceptor membrane, to its hydroperoxide (22:6HP). During this phase, microglia migrate to the site of photoreceptors. T...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-0221
更新日期：2006-08-01 00:00:00
abstract：Purpose:To investigate by optical coherence tomography angiography (OCT-A) the choroidal vascular response to experimentally increased blood pressure in patients with central serous chorioretinopathy (CSCR). Methods:For this multicenter, observational, case-control study, we enrolled 35 patients with an established di...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,多中心研究
doi：10.1167/iovs.18-23995
更新日期：2018-08-01 00:00:00
abstract：PURPOSE:To determine the levels of free UV filters and selected amino acids in cataract lenses compared with normal lenses. METHODS:Indian cataract lenses (n=39) and normal lenses (n=6) were examined by HPLC to quantify levels of UV filter compounds, the UV filter precursor amino acid tryptophan (Trp), as well as tyro...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.04-0178
更新日期：2004-11-01 00:00:00
abstract：:Electroretinographic studies of myctalopic Appaloosa horses demonstrated photopic and scotopic abnormalities similar to those in humans with congenital stationary night blindness (CSNB) of the Schubert-Bornschein type. The phototopic abnormalities consisted of reduced b-wave amplitudes and slower than normal b-wave im...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
abstract：PURPOSE:Ibuprofen and indomethacin are nonselective prostaglandin synthetase inhibitors that have been shown to improve oxygen-induced retinopathy in mice. Vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF)-I, and growth hormone (GH) are potent growth factors involved in retinal development. Th...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-0057
更新日期：2006-07-01 00:00:00
abstract：:Interactions between direction-selective mechanisms for motion were examined in strabismic and anisometropic amblyopia. Amblyopes were found to have a marked reduction in sensitivity to flicker or movement when the amblyopic eye was presented with a combination of two gratings drifting in opposite directions at the sa...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:N-formimidoyl thienamycin (MK-787) is a new beta-lactam with potent activity against both aerobic and anaerobic gram-positive and gram-negative bacteria. Its spectrum and activity suggest it may be useful in treatment of complicated intraocular infections. Its ocular penetration was studied in New Zealand white rabbit...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：PURPOSE:To isolate all constituent mucins from human conjunctival mucus. METHODS:Mucins were extracted from human conjunctiva in guanidine hydrochloride and protease inhibitors. The mucins were isolated by density gradient centrifugation, gel filtration, and ion exchange chromatography. Throughout purification, the mu...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：Purpose:The cone-rod homeobox (CRX) transcription factor is essential for photoreceptor gene expression, differentiation, and survival. Human CRX mutations can cause dominant retinopathies of varying onset and phenotype severity. In animal models, dominant frameshift Crx mutations introduce a premature termination codo...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-22075
更新日期：2017-09-01 00:00:00
abstract：Purpose:Uveal melanoma (UM) is the most common primary intraocular malignancy in adults and approximately half of those diagnosed will die of metastasis. This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed "cancer stem cells" (CSCs). Methods:Expression of postulated s...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-22272
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:Point and null mutations in interphotoreceptor retinoid-binding protein (IRBP) cause retinal dystrophy in affected patients and IRBP-deficient mice with unknown mechanism. This study investigated whether IRBP protects cells from damages induced by all-trans-retinal (atRAL), which was increased in the Irbp(-/-) ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.15-18551
更新日期：2016-04-01 00:00:00
abstract：PURPOSE:To examine the expression of matrix metalloproteinases (MMPs) and their inhibitors during the development of retinal neovascularization (NV) in a mouse model. METHODS:A well-characterized murine model of retinal NV was used to study the expression of specific MMPs (MMP-2, MMP-9, and MT1-MMP) and tissue inhibit...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Purpose. To use optical coherence tomography (OCT) to evaluate the effect of punctal occlusion on tear meniscus volume in dry eye patients. Methods. Occlusion of both upper and lower puncta with collagen plugs was performed on one eye each in 20 dry eye patients and 20 normal subjects. The upper and lower tear menisci...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-4349
更新日期：2010-04-01 00:00:00
abstract：PURPOSE:CD4(+)CD25(+)FoxP3(+) naturally occurring regulatory T cells (Tregs) maintain self-tolerance and function to suppress overly exuberant immune responses. However, it is unclear whether innate immune cells modulate Treg function. Here the authors examined the role of innate immunity in lymphomyeloid homeostasis. ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6536
更新日期：2011-04-08 00:00:00
abstract：PURPOSE:To determine the effect of Latrunculin (LAT)-A, a macrolide that binds to G-actin, which leads to the disassembly of actin filaments, on shape, junctions, and the cytoskeleton of cultured bovine aortic endothelial cells (BAECs) and on outflow facility in living monkeys. METHODS:Latrunculin-A dose-time-response...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：Purpose:The purpose of this study was to evaluate macular preganglionic function and to verify its relationship with retinal and choroidal morphology in patients with intermediate age-related macular degeneration (iAMD) patients. Methods:All included patients performed multifocal electroretinogram (mfERG) for investig...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.61.5.11
更新日期：2020-05-11 00:00:00
abstract：PURPOSE:Current understanding of the genetic risk factors for age-related macular degeneration (AMD) is not sufficiently predictive of the clinical course. The VEGF pathway is a key therapeutic target for treatment of neovascular AMD; however, risk attributable to genetic variation within pathway genes is unclear. We s...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.14-14047
更新日期：2014-05-08 00:00:00
