Multiple Differentially Methylated Regions Specific to Keratoconus Explain Known Keratoconus Linkage Loci.

abstract：PURPOSE:To characterize the reactions of retinal glial cells (astrocytes and Müller cells) to retinal injury in mice that lack glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) and to determine the role of glial cells in retinal detachment (RD)-induced photoreceptor degeneration. METHODS:RD was induc...

journal_title：Investigative ophthalmology & visual science

authors： Nakazawa T,Takeda M,Lewis GP,Cho KS,Jiao J,Wilhelmsson U,Fisher SK,Pekny M,Chen DF,Miller JW

更新日期：2007-06-01 00:00:00

abstract：PURPOSE:To examine the effect of combining angiostatin with photodynamic therapy (PDT) using Lutetium Texaphyrin (Lu-Tex; Alcon, Fort Worth, TX) as a photosensitizer in bovine retinal capillary endothelial (BRCE) and retinal pigment epithelial (RPE) cells and to determine the mode of PDT-induced cell death in these cel...

journal_title：Investigative ophthalmology & visual science

authors： Renno RZ,Delori FC,Holzer RA,Gragoudas ES,Miller JW

更新日期：2000-11-01 00:00:00

abstract：Purpose:IFT81, a core component of the IFT-B complex, involved in the bidirectional transport of ciliary proteins, has been recently implicated in syndromic ciliopathies. However, none of the IFT-B core complex proteins have been associated with nonsyndromic retinal dystrophies. Given the importance of ciliary transpor...

journal_title：Investigative ophthalmology & visual science

authors： Dharmat R,Liu W,Ge Z,Sun Z,Yang L,Li Y,Wang K,Thomas K,Sui R,Chen R

更新日期：2017-05-01 00:00:00

abstract：PURPOSE:To assess corneal epithelial cell survival after keratoplasty. METHODS:Corneal impression cytology (CIC) was performed on sex-mismatched corneal transplants. Fluorescent in situ hybridization (FISH) with sex chromosome-specific probes was performed to identify epithelial cell mosaicism and therefore allocate t...

journal_title：Investigative ophthalmology & visual science

authors： Catanese M,Popovici C,Proust H,Hoffart L,Matonti F,Cochereau I,Conrath J,Gabison EE

更新日期：2011-02-22 00:00:00

abstract：PURPOSE:The secreted Ly6/uPAR-related protein-1 (Slurp1), associated with the hyperkeratotic disorder mal de Meleda, is abundantly expressed in corneas. Here, we examine its corneal expression and functions. METHODS:Gene expression was quantified by quantitative PCR (qPCR), immunoblots, and immunofluorescent staining....

journal_title：Investigative ophthalmology & visual science

authors： Swamynathan S,Buela KA,Kinchington P,Lathrop KL,Misawa H,Hendricks RL,Swamynathan SK

更新日期：2012-12-19 00:00:00

abstract：Purpose:The purpose of this study was to establish and analyze a cell model of Leber congenital amaurosis type 16 (LCA16), which is caused by mutations in the KCNJ13 gene encoding Kir7.1, an inward-rectifying potassium ion channel. Methods:The two guide RNAs specific to the target sites in the KCNJ13 gene were designe...

journal_title：Investigative ophthalmology & visual science

authors： Kanzaki Y,Fujita H,Sato K,Hosokawa M,Matsumae H,Shiraga F,Morizane Y,Ohuchi H

更新日期：2020-05-11 00:00:00

abstract：PURPOSE:To describe and evaluate a new experimental reference plane for measuring rim area in scanning laser tomography. METHODS:The experimental reference plane was positioned so that (1) it always lay entirely below the margin of the optic nerve head (ONH), (2) it remained at a set z-axis distance below the ONH in i...

journal_title：Investigative ophthalmology & visual science

authors： Tan JC,Hitchings RA

更新日期：2003-03-01 00:00:00

abstract：PURPOSE:To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline. METHODS:A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Kr...

journal_title：Investigative ophthalmology & visual science

authors： Chikama T,Hayashi Y,Liu CY,Terai N,Terai K,Kao CW,Wang L,Hayashi M,Nishida T,Sanford P,Doestchman T,Kao WW

更新日期：2005-06-01 00:00:00

abstract：PURPOSE:A prior study showed that cholinergic agonists activate phospholipase D (PLD). The purpose of this study was to determine whether cholinergic agonists use the PLD pathway to alter protein secretion and to identify the molecular signaling components of this pathway in rat lacrimal gland acini. METHODS:Rat lacri...

journal_title：Investigative ophthalmology & visual science

authors： Hodges RR,Guilbert E,Shatos MA,Natarajan V,Dartt DA

更新日期：2011-04-06 00:00:00

abstract：PURPOSE:To develop and characterize a feline model of retinal degeneration induced by intravitreal injection of adenosine triphosphate (ATP). METHODS:Nineteen normally sighted adult cats received 100 μL intravitreal injections of ATP with a final concentration of 11, 22, or 55 mM at the retina. Four animals were eutha...

journal_title：Investigative ophthalmology & visual science

authors： Aplin FP,Luu CD,Vessey KA,Guymer RH,Shepherd RK,Fletcher EL

更新日期：2014-11-25 00:00:00

abstract：PURPOSE:To investigate the alterations in microRNA (miRNA) expression during replicative senescence (RS) in human trabecular meshwork (HTM) cells. METHODS:Two HTM cell lines were serially passaged until they reached RS. Changes in expression of 30 miRNAs were assessed by real-time quantitative (q)-PCR. The effects of ...

journal_title：Investigative ophthalmology & visual science

authors： Li G,Luna C,Qiu J,Epstein DL,Gonzalez P

更新日期：2010-06-01 00:00:00

abstract：PURPOSE:To examine the expression of Angiotensin II (Ang II) and its type I, and type II receptors (AT1R, AT2R) in rabbit Tenon's capsule fibroblasts after trabeculectomy, and to investigate the effects of Ang II on cultured human Tenon's capsule fibroblasts (HTFs) proliferation, migration, phenotype transition, and ex...

journal_title：Investigative ophthalmology & visual science

authors： Shi H,Zhang Y,Fu S,Lu Z,Ye W,Xiao Y

更新日期：2015-01-06 00:00:00

abstract：PURPOSE:We investigated the effects of semaphorin 3E (Sema3E)/PlexinD1 signaling in the development of choroidal neovascularization (CNV) and explored the therapeutic potential of the pathway. METHODS:We used a laser-induced CNV model in the mouse. The expression of Sema3E and PlexinD1 was evaluated with immunohistoch...

journal_title：Investigative ophthalmology & visual science

authors： Suda K,Guo C,Oishi A,Ikeda S,Uemura A,Yoshimura N

更新日期：2014-07-01 00:00:00

abstract：:A monoclonal antibody has been produced that binds to the apical squames (flattened cells) of the rat ocular surface epithelium and to the goblet cells of the conjunctiva. Immunoelectron microscopic localization of the antigen indicates that in apical cells it is present along the apical-microplical membrane in the re...

journal_title：Investigative ophthalmology & visual science

authors： Gipson IK,Yankauckas M,Spurr-Michaud SJ,Tisdale AS,Rinehart W

更新日期：1992-01-01 00:00:00

abstract：Purpose:Examine associations between the vasculature at arteriovenous (AV) crossings and the onset of branch retinal vein occlusion (BRVO). Methods:We included 78 patients with major BRVO, 35 patients with macular BRVO, and 110 controls without BRVO and determined the vessel positions at AV crossings, where the first-...

journal_title：Investigative ophthalmology & visual science

authors： Kogo T,Muraoka Y,Iida Y,Ooto S,Murakami T,Kadomoto S,Iida-Miwa Y,Numa S,Miyake M,Miyata M,Uji A,Tsujikawa A

更新日期：2020-02-07 00:00:00

abstract：:N-formimidoyl thienamycin (MK-787) is a new beta-lactam with potent activity against both aerobic and anaerobic gram-positive and gram-negative bacteria. Its spectrum and activity suggest it may be useful in treatment of complicated intraocular infections. Its ocular penetration was studied in New Zealand white rabbit...

journal_title：Investigative ophthalmology & visual science

authors： Finlay KR,Carlson CL,Chow AW

更新日期：1983-08-01 00:00:00

abstract：PURPOSE:To evaluate a novel objective perimetry using multifocal chromatic pupil light reflex in normal participants and patients with photoreceptor dysfunction, and to relate this new technique with subjective dark-adapted chromatic Goldmann perimetry. METHODS:Thirty-two eyes of 17 retinitis pigmentosa (RP) or cone-r...

journal_title：Investigative ophthalmology & visual science

authors： Skaat A,Sher I,Kolker A,Elyasiv S,Rosenfeld E,Mhajna M,Melamed S,Belkin M,Rotenstreich Y

更新日期：2013-04-17 00:00:00

abstract：:The adhesion complex of the corneal epithelium consists of the hemidesmosome and its associated structures, such as anchoring filaments, lamina densa of the basement membrane, and anchoring fibrils. It contributes to the adhesion of the corneal epithelium to Bowman's layer. To understand the adhesion complex better, a...

journal_title：Investigative ophthalmology & visual science

authors： Stock EL,Kurpakus MA,Sambol B,Jones JC

更新日期：1992-02-01 00:00:00

abstract：PURPOSE:The link between nasal-temporal motion asymmetries and anomalous binocular sensory function in infantile esotropia (ET) has led to the idea that visual evoked potential responses to horizontal motion (mVE) is an alternative measure of sensory binocularity to stereopsis. A second hypothesis is that the mVEP resp...

journal_title：Investigative ophthalmology & visual science

authors： Fawcett SL,Birch EE

更新日期：2000-02-01 00:00:00

abstract：PURPOSE:To investigate the hypothesis that tumor-infiltrating macrophages contribute to prognosis of uveal melanoma and to study their association with tumor characteristics, especially microvessels. METHODS:This was a retrospective, population-based cohort study of 167 consecutive patients who had had an eye with cho...

journal_title：Investigative ophthalmology & visual science

authors： Mäkitie T,Summanen P,Tarkkanen A,Kivelä T

更新日期：2001-06-01 00:00:00

abstract：:PURPOSE. Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of retina photoreceptors. Ceramide-1-pho...

journal_title：Investigative ophthalmology & visual science

authors： Miranda GE,Abrahan CE,Agnolazza DL,Politi LE,Rotstein NP

更新日期：2011-08-22 00:00:00

abstract：PURPOSE:To evaluate the potential role that FoxO transcription factors play in modulating resveratrol's protective effects against oxidative stress in lens epithelial cells. METHODS:Primary human or porcine lens epithelial cells (LECs) were treated with resveratrol (RES) 25 μM and incubated under either physiologic (5...

journal_title：Investigative ophthalmology & visual science

authors： Li G,Luna C,Navarro ID,Epstein DL,Huang W,Gonzalez P,Challa P

更新日期：2011-06-21 00:00:00

abstract：PURPOSE:The adult lacrimal gland (LG) is highly regenerative and is able to repair itself even after substantial damage; however, this ability to regenerate is lost with the development of dry eye conditions in chronically inflamed LGs.This study compares changes in the cell adhesion and cell matrix molecules and stem ...

journal_title：Investigative ophthalmology & visual science

authors： Umazume T,Thomas WM,Campbell S,Aluri H,Thotakura S,Zoukhri D,Makarenkova HP

更新日期：2015-12-01 00:00:00

abstract：PURPOSE:To investigate in impression cytology (IC) specimens the expression of inflammatory and apoptosis-related markers by conjunctival epithelial cells from patients with dry eye as a rationale for treatment with topical cyclosporine. METHODS:Immunologic anomalies were identified at baseline, before treatment with ...

journal_title：Investigative ophthalmology & visual science

authors： Brignole F,Pisella PJ,Goldschild M,De Saint Jean M,Goguel A,Baudouin C

更新日期：2000-05-01 00:00:00

abstract：PURPOSE:To quantify and compare the structural and functional changes in subjects with early age-related macular degeneration (AMD), using spectral-domain optical coherence tomography (SD-OCT) and microperimetry. METHODS:Twenty-one eyes of 21 subjects with early AMD were examined. MP-1 10-2 visual fields (VFs) and SD-...

journal_title：Investigative ophthalmology & visual science

authors： Acton JH,Smith RT,Hood DC,Greenstein VC

更新日期：2012-11-09 00:00:00

abstract：Purpose:The purpose of this study was to study the status and association among tear-soluble factors, corneal dendritic cell density, vitamin D, and signs and symptoms in dry eye disease (DED). Methods:A total of 33 control subjects and 47 evaporative dry eye patients were included in the study. DED diagnosis and clas...

journal_title：Investigative ophthalmology & visual science

authors： Khamar P,Nair AP,Shetty R,Vaidya T,Subramani M,Ponnalagu M,Dhamodaran K,D'souza S,Ghosh A,Pahuja N,Deshmukh R,Ahuja P,Sainani K,Nuijts RMMA,Das D,Ghosh A,Sethu S

更新日期：2019-06-03 00:00:00

abstract：:Freshly enucleated monkey, calf, and human eyes were quantitatively perfused with mock aqueous humor through the anterior chamber by a constant pressure technique. After baseline perfusion at 15 mm Hg, intraocular pressure was raised to 45 mm Hg and later reduced back to 15 mm Hg. Calf and human (both adult and infant...

journal_title：Investigative ophthalmology & visual science

authors： Hashimoto JM,Epstein DL

更新日期：1980-12-01 00:00:00

abstract：PURPOSE:A great deal of information about functionally significant domains of a protein may be obtained by comparison of primary sequences of gene homologues over a broad phylogenetic base. This study was designed to identify evolutionarily conserved domains of the photoreceptor disc membrane protein peripherin/rds by ...

journal_title：Investigative ophthalmology & visual science

authors： Li C,Ding XQ,O'Brien J,Al-Ubaidi MR,Naash MI

更新日期：2003-06-01 00:00:00

abstract：PURPOSE:To assess the impact of amblyopia, strabismus and glasses on subjective visual and psychological function among amblyopes. METHODS:Questionnaires were administered to 120 teenagers with amblyopia (cases), with residual amblyopia after treatment, or with or without strabismus and 120 control subjects (controls)...

journal_title：Investigative ophthalmology & visual science

authors： Sabri K,Knapp CM,Thompson JR,Gottlob I

更新日期：2006-10-01 00:00:00

abstract：PURPOSE:Somatostatin (SST) analogues have been used to treat proliferative diabetic retinopathy, pseudotumor cerebri, thyroid orbitopathy, and cystoid macular edema. There is a paucity of published data in regards to cell-specific distribution of SST receptors (SSTR) in normal human eye tissues. Gene expression for all...

journal_title：Investigative ophthalmology & visual science

authors： Klisovic DD,O'Dorisio MS,Katz SE,Sall JW,Balster D,O'Dorisio TM,Craig E,Lubow M

更新日期：2001-09-01 00:00:00