Abstract：
:The effects of exogenous GM1 and GD1a on S20Y murine neuroblastoma cells were assessed by monitoring morphology, tumorigenicity, mitotic index, and plating efficiency. S20Y cells were seeded at a density equivalent to 5 X 10(4) cells per 35-mm tissue culture dish; 38-42 hr after seeding (preconfluent stage) the cells were treated for 12 hr with 100 micrograms of ganglioside per ml of medium in which the serum content was reduced from 10% to 0.5%. Analysis of the cell lipids indicated that added ganglioside became tightly associated with the membrane during the 12-hr exposure. GM1 treatment resulted in increased projections on the cell surface and fine structures projecting from the cell processes. GD1a treatment resulted in a reduction in the cellular mitotic index. Plating efficiency was reduced by both GM1 and GD1a. Neither ganglioside affected tumorigenicity of the S20Y cells. Twelve hours after removal of the added ganglioside and exposure of the cells to normal medium, the ganglioside composition of the membranes from treated cells approached that of the controls, and the ganglioside-induced effects had been reversed. These results suggest that addition of specific gangliosides induces different cellular responses and that these changes are dependent upon the continued presence of the ganglioside.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Carine K,Schengrund CLdoi
10.1002/jnr.490120106subject
Has Abstractpub_date
1984-01-01 00:00:00pages
59-69issue
1eissn
0360-4012issn
1097-4547journal_volume
12pub_type
杂志文章abstract：:We studied the postischemic alterations of second messenger and receptor systems focusing on the strionigral pathway in order to clarify the mechanism of the delayed neuronal changes in remote areas of the rat brain after transient focal ischemia. Chronological changes of [3H]forskolin and [3H]SCH 23390 binding sites ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330315
更新日期：1992-11-01 00:00:00
abstract：:There are two types of calcium-activated neutral protease (CANP), m-CANP and mu-CANP, following the nomenclature of Suzuki et al to show that each requires mM and microM Ca2+, respectively, for its activation. We found mu-CANP activity in a crude CANP fraction extracted from the peripheral nerve, which degraded the ne...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130306
更新日期：1985-01-01 00:00:00
abstract：:The cellular localization of calpain is important in understanding the roles that calpain may play in physiological function. We, therefore, examined calpain expression, activity, and immunofluorescent localization in primary cultures of rat oligodendrocytes. The mRNA expression of m-calpain was 64.8% (P = 0.0033) and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10414
更新日期：2002-11-15 00:00:00
abstract：:The Brn-3a transcription factor is critical for survival and differentiation of sensory neurons derived from neural crest cells (NCC). Interaction of Brn-3a with p53 results in differential effects on target gene expression, which profoundly affects fate of neuronal cells. Here we demonstrate colocalization of p53 in ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20299
更新日期：2004-12-15 00:00:00
abstract：:Most cases of early-onset torsion dystonia are caused by deletion of GAG in the coding region of the DYT1 gene encoding torsinA. This autosomal dominant neurologic disorder is characterized by abnormal movements, believed to originate from neuronal dysfunction in the basal ganglia of the human brain. The torsins (tors...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10567
更新日期：2003-04-15 00:00:00
abstract：:Metallothionein (MT) belongs to a family of metal-binding cysteine-rich proteins comprising several structurally related proteins implicated in tissue protection and regeneration after injuries and functioning as antiapoptotic antioxidants in neurological disorders. This has been demonstrated in animals receiving MT t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22118
更新日期：2009-10-01 00:00:00
abstract：:In this report, we address the molecular mechanisms that regulate axonal growth by focusing on the gene for one of the major axonal cytoskeletal proteins, T alpha1 alpha-tubulin. During the developmental growth of sympathetic neurons, transcription of a beta-galactosidase transgene driven by the T alpha1 promoter (T a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:The transplantation of dopaminergic (DA) progenitors derived from pluripotent stem cells improves the behavior of Parkinson's disease model animals. However, the survival of DA progenitors is low, and the final yield of DA neurons is only approximately 0.3%-2% the number of transplanted cells. Zonisamide (ZNS) increas...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24668
更新日期：2020-08-01 00:00:00
abstract：:Understanding the pathway for amyloid percursor protein (APP) catabolism has become an important line of investigation. APP is a ubiquitous membrane bound protein that is rapidly cleaved at the membrane, yielding a secreted protein identical to protease nexin II and an internalized 11.5 kDa 100 residue C terminal deri...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330121
更新日期：1992-09-01 00:00:00
abstract：:Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490150312
更新日期：1986-01-01 00:00:00
abstract：:We have examined the expression and distribution of the extracellular matrix molecule tenascin-C in and around lesions of the thoracic dorsal columns in adult rats 3 days to 8 weeks after injury, using in situ hybridization, immunofluorescence, electron microscopy and immunoelectron microscopy. Numerous tenascin-C mRN...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-08-15 00:00:00
abstract：:The periglomerular cells (PG) of the olfactory bulb (OB) are involved in the primary processing and the refinement of sensory information from the olfactory epithelium. The neurochemical composition of these neurons has been studied in depth in many species, and over the last decades such studies have focused mainly o...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22521
更新日期：2011-01-01 00:00:00
abstract：:In vertebrates and invertebrates, dopamine signaling modulates a wide variety of physical and behavioral functions and exerts these effects through heterotrimeric G proteins. The soil nematode Caenorhabditis elegans has been used to model dopamine signaling and reacts reproducibly to alterations in dopamine levels thr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22694
更新日期：2011-10-01 00:00:00
abstract：:Spinocerebellar ataxia type 17 (SCA17) is a type of autosomal dominant cerebellar ataxia (ADCA) characterized by variable manifestations, including cerebellar ataxia, dementia, and psychiatric symptoms. Since the identification of a CAG repeat expansion in the TATA-box binding protein (TBP) gene in a patient with atax...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23984
更新日期：2017-08-01 00:00:00
abstract：:Previous studies have shown that the mechanical wounding of 3-week-old cultured rat astrocytes results in cell proliferation and hypertrophy resembling astrocyte responses to a brain injury in vivo. We now report the effects of basic fibroblast growth factor (bFGF) and an anti-bFGF antibody on astrocyte morphology, pr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400310
更新日期：1995-02-15 00:00:00
abstract：:Diabetes and obesity are characterized by hyperlipidemia and represent risk factors for premature neurological disorders. Diabetic/obese animals have impaired adult neurogenesis. We hypothesize that lipotoxicity leading to neurogenesis impairment plays a role in the development of neurological complications. If so, no...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22803
更新日期：2012-04-01 00:00:00
abstract：:Although upregulation of ryanodine receptor (RyR)-1 and -2 is mediated through the activation of dopamine D1 receptors (D1DRs) in the development of psychostimulant-induced place preference, little is known about how such increased expressions of RyRs are negatively regulated. This study investigated negative regulato...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23058
更新日期：2012-08-01 00:00:00
abstract：:Nicotinates, pantothenates, riboflavin, vitamins B6 and B12' free (acetyl) and total (free and bound) choline, biopterin, thiamin, biotin, methylated and nonmethylated folates in frontal, temporal, precentral, postcentral, and occipital cortex, thalamus, cerebellum, pons, basal ganglia, and substantia nigra were estim...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110409
更新日期：1984-01-01 00:00:00
abstract：:The N-methyl-D-aspartate receptor (NMDAR) is a major neurotransmitter receptor in the central nervous system (CNS), with functional roles in learning, memory, and sensation. Several mechanisms potentiate NMDARs, and NMDAR hyperexcitability plays pathophysiological roles in many conditions, such as neurodegenerative di...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10793
更新日期：2003-12-01 00:00:00
abstract：:The effects of ischemia (removal of oxygen and glucose for 4 h) and anoxia (removal of oxygen alone) on astrocytes were studied in dissociated cultures of E14 spinal cord containing both neurons and astrocytes. In addition, a group of cultures was treated with a low Na+, low Ca2+, and high K+ medium during the 4-h isc...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490170212
更新日期：1987-01-01 00:00:00
abstract：:Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characteriz...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20645
更新日期：2005-11-15 00:00:00
abstract：:Cerebral vasospasm is a potentially incapacitating or lethal complication in patients with aneurysmal subarachnoid hemorrhage (SAH). The development of effective preventative and therapeutic interventions has been largely hindered by the fact that the underlying pathogenic mechanisms of cerebral vasospasm remain poorl...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.21823
更新日期：2009-01-01 00:00:00
abstract：:A previous study demonstrated the release of newly loaded radiolabelled taurine (Tau) from cultured rabbit Müller glia not only following typical cell volume-increasing treatments with high (65 mM) potassium ions or hypotonic media, but also with ammonium chloride (further referred to as ammonia), in a dose-dependent ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961015)46:2<231::AID-JNR
更新日期：1996-10-15 00:00:00
abstract：:The hippocampus is an important brain region involved in memory and learning. Brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor B (TrkB), and phospho-p44/p42 mitogen-activated protein kinase (MAPK) are known to contribute to hippocampal memory/learning. The present study aimed to clarify the effects o...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24216
更新日期：2018-06-01 00:00:00
abstract：:Previously, we showed that in rat cortical neurons, chronic donepezil treatment (10 microM, 4 days) up-regulates nicotinic receptors (nAChR) and makes neurons more sensitive to the neuroprotective effect of donepezil. Here we examined the mechanism of donepezil-induced neuroprotection in neurons chronically treated wi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21798
更新日期：2008-12-01 00:00:00
abstract：:Amyotrophic lateral sclerosis is an age-related neurological disease, characterized by neurofilament (NF) accumulation in primary axons followed by degeneration of motor neurons. To elucidate age-related factors that might lead to pathological NF accumulation, NFs were compared between young and aged rats. Electron mi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-10-15 00:00:00
abstract：:The human immunodeficiency virus type 1 (HIV-1) glycoprotein gp120 causes neuronal cell death; however, the molecular mechanisms of the neurotoxic effect remain largely unresolved. It has been suggested that gp120 evokes cell death by inducing the release of neurotoxins, including glutamate. The objective of this work...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10826
更新日期：2004-01-01 00:00:00
abstract：:Transgenic mice with glial fibrillary acidic protein (GFAP) promoter driven-astrocyte production of the cytokines interleukin-6 (IL-6) and tumor necrosis factor (TNF) were used to determine whether the pre-existing production of these cytokines in vivo might modulate the sensitivity of neurons to excitotoxic agents. L...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10635
更新日期：2003-07-15 00:00:00
abstract：:Microglia are prominently involved in neural degenerative diseases of the CNS and the retina. In this study, we determined the activation and phagocytotic function of different subtypes of retinal microglial cells at 1 week and 1 month following optic axotomy. Fluorescent DiI crystals were placed at the stumps of the ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10713
更新日期：2003-09-15 00:00:00
abstract：:Cell death in outer hair cells of the mammalian inner ear induced by aminoglycoside antibiotics is mediated by reactive oxygen species (ROS) and can be prevented by antioxidants. The current study investigates the role of the nuclear factor (NF)-kappaB pathway in cell death or survival in adult CBA mice. Kanamycin (70...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20392
更新日期：2005-03-01 00:00:00
