当前位置： SCI文献检索 > NATURE期刊下所有文献 > Functional V region formation during in vitro culture of a murine immature B precursor cell line.

Functional V region formation during in vitro culture of a murine immature B precursor cell line.

Abstract：

:B lymphocytes originate from pluripotential haematopoietic stem cells and differentiate into immunoglobulin (Ig)-producing cells. B-cell lineage differentiation is accompanied by two types of immunoglobulin gene rearrangements--rearrangement of V, D and J gene segments to create a functional V gene and rearrangement of CH genes for heavy-chain switching. These results, however, have been obtained mainly by analysis of immunoglobulin gene organization of myeloma cells. Baltimore and his colleagues have established Abelson murine leukaemia virus (A-MuLV)-transformed cell lines and found a few lines capable of carrying out kappa-gene rearrangement or undergoing isotype switching during in vitro culture. To study early B-cell lineage differentiation events, we have now also established A-MuLV-transformed cell lines which are capable of differentiating from mu- to mu+ and of undergoing continuing rearrangement of heavy-chain genes in culture. Analysis of immunoglobulin gene organization of these transformed cells revealed that mu- cells have already undergone DNA rearrangements involving JH segments but an additional rearrangement of JH segments is required for initiation of mu-chain synthesis. Southern blot analysis of the DNA and two-dimensional gel electrophoresis of intracytoplasmic mu-chain show that mu-chain diversity with respect to antigen specificity may be generated during this second rearrangement process. As no rearrangement of light-chain genes takes place in these cells, this implies that light-chain gene rearrangement requires some further change, or a different enzyme.

journal_name

Nature

journal_title

Nature

authors

Sugiyama H,Akira S,Kikutani H,Kishimoto S,Yamamura Y,Kishimoto T

doi

10.1038/303812a0

subject

Has Abstract

pub_date

1983-06-30 00:00:00

pages

812-5

issue

5920

eissn

0028-0836

issn

1476-4687

journal_volume

303

pub_type

杂志文章

相关文献

NATURE文献大全
  • Comparison of human genetic and sequence-based physical maps.

    abstract：:Recombination is the exchange of information between two homologous chromosomes during meiosis. The rate of recombination per nucleotide, which profoundly affects the evolution of chromosomal segments, is calculated by comparing genetic and physical maps. Human physical maps have been constructed using cytogenetics, o...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35057185

    authors： Yu A,Zhao C,Fan Y,Jang W,Mungall AJ,Deloukas P,Olsen A,Doggett NA,Ghebranious N,Broman KW,Weber JL

    更新日期：2001-02-15 00:00:00

  • Possible role for salivary gland protein in taste reception indicated by homology to lipophilic-ligand carrier proteins.

    abstract：:Sensory transduction in taste and olfaction, the principal chemical senses, seems to be mediated by membrane-associated proteins on the apical surfaces of the respective receptor cells. The recent isolation and characterization of soluble 'odorant-binding proteins' secreted from the nasal glands of rat, cow and frog, ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/343366a0

    authors： Schmale H,Holtgreve-Grez H,Christiansen H

    更新日期：1990-01-25 00:00:00

  • Dopamine D2 receptors in discrimination learning and spine enlargement.

    abstract：:Dopamine D2 receptors (D2Rs) are densely expressed in the striatum and have been linked to neuropsychiatric disorders such as schizophrenia1,2. High-affinity binding of dopamine suggests that D2Rs detect transient reductions in dopamine concentration (the dopamine dip) during punishment learning3-5. However, the natur...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-020-2115-1

    authors： Iino Y,Sawada T,Yamaguchi K,Tajiri M,Ishii S,Kasai H,Yagishita S

    更新日期：2020-03-01 00:00:00

  • Recruitment of cytosolic proteins to a secretory granule membrane depends on Ca2+-calmodulin.

    abstract：:An increase in free calcium triggers catecholamine secretion from chromaffin cells and calmodulin is strongly implicated as the intracellular Ca2+ receptor. In our recent studies of calmodulin action in the chromaffin cell, micromolar Ca2+ concentrations resulted in calmodulin and cytosolic proteins becoming bound to ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/301432a0

    authors： Geisow MJ,Burgoyne RD

    更新日期：1983-02-03 00:00:00

  • Perovskite oxides for visible-light-absorbing ferroelectric and photovoltaic materials.

    abstract：:Ferroelectrics have recently attracted attention as a candidate class of materials for use in photovoltaic devices, and for the coupling of light absorption with other functional properties. In these materials, the strong inversion symmetry breaking that is due to spontaneous electric polarization promotes the desirab...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature12622

    authors： Grinberg I,West DV,Torres M,Gou G,Stein DM,Wu L,Chen G,Gallo EM,Akbashev AR,Davies PK,Spanier JE,Rappe AM

    更新日期：2013-11-28 00:00:00

  • Seeing mesoatomic distortions in soft-matter crystals of a double-gyroid block copolymer.

    abstract：:Supramolecular soft crystals are periodic structures that are formed by the hierarchical assembly of complex constituents, and occur in a broad variety of 'soft-matter' systems1. Such soft crystals exhibit many of the basic features (such as three-dimensional lattices and space groups) and properties (such as band str...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-019-1706-1

    authors： Feng X,Burke CJ,Zhuo M,Guo H,Yang K,Reddy A,Prasad I,Ho RM,Avgeropoulos A,Grason GM,Thomas EL

    更新日期：2019-11-01 00:00:00

  • Rise of the temporary workforce.

    abstract：: ...

    journal_title：Nature

    pub_type： 评论,新闻

    doi：10.1038/d41586-019-00450-0

    authors： Gewin V

    更新日期：2019-02-01 00:00:00

  • Innate immunity and intestinal microbiota in the development of Type 1 diabetes.

    abstract：:Type 1 diabetes (T1D) is a debilitating autoimmune disease that results from T-cell-mediated destruction of insulin-producing beta-cells. Its incidence has increased during the past several decades in developed countries, suggesting that changes in the environment (including the human microbial environment) may influe...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature07336

    authors： Wen L,Ley RE,Volchkov PY,Stranges PB,Avanesyan L,Stonebraker AC,Hu C,Wong FS,Szot GL,Bluestone JA,Gordon JI,Chervonsky AV

    更新日期：2008-10-23 00:00:00

  • TFIIIC relieves repression of U6 snRNA transcription by chromatin.

    abstract：:The U6 small nuclear (sn)RNA gene (SNR6) from the yeast Saccharomyces cerevisiae is transcribed by RNA polymerase III in vivo. This gene is unusual in having a TATA box at position -30, and an essential B-block element located downstream of the T-rich termination signal. The B block is one of the two intragenic promot...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/362475a0

    authors： Burnol AF,Margottin F,Huet J,Almouzni G,Prioleau MN,Méchali M,Sentenac A

    更新日期：1993-04-01 00:00:00

  • The cellular prion protein binds copper in vivo.

    abstract：:The normal cellular form of prion protein (PrPC) is a precursor to the pathogenic protease-resistant forms (PrPSc) believed to cause scrapie, bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease. Its amino terminus contains the octapeptide PHGGGWGQ, which is repeated four times and is among the best-pr...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/37783

    authors： Brown DR,Qin K,Herms JW,Madlung A,Manson J,Strome R,Fraser PE,Kruck T,von Bohlen A,Schulz-Schaeffer W,Giese A,Westaway D,Kretzschmar H

    更新日期：1997-12-18 00:00:00

  • Transposon tagging of a male sterility gene in Arabidopsis.

    abstract：:Transformation of the well-studied maize transposable elements into other plant species should enable transposon tagging methodology to be used for the isolation of interesting genes in the heterologous host. Here we describe the isolation of a transposon-tagged male sterile mutant in Arabidopsis thaliana using the ma...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/363715a0

    authors： Aarts MG,Dirkse WG,Stiekema WJ,Pereira A

    更新日期：1993-06-24 00:00:00

  • Rate of turnover of structural variants in the rDNA gene family of Drosophila melanogaster.

    abstract：:A high degree of polymorphism for the length and copy number of rDNA spacers, in both the X and Y chromosome clusters, has been found in a wild population of Drosophila melanogaster. The genetic behaviour of rDNA structural variants in separate and mixed populations derived from isofemale lines suggest that they are n...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/295564a0

    authors： Coen ES,Thoday JM,Dover G

    更新日期：1982-02-18 00:00:00

  • 5-HT release in nucleus accumbens rescues social deficits in mouse autism model.

    abstract：:Dysfunction in prosocial interactions is a core symptom of autism spectrum disorder. However, the neural mechanisms that underlie sociability are poorly understood, limiting the rational development of therapies to treat social deficits. Here we show in mice that bidirectional modulation of the release of serotonin (5...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-018-0416-4

    authors： Walsh JJ,Christoffel DJ,Heifets BD,Ben-Dor GA,Selimbeyoglu A,Hung LW,Deisseroth K,Malenka RC

    更新日期：2018-08-01 00:00:00

  • Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.

    abstract：:The alternative non-homologous end-joining (NHEJ) machinery facilitates several genomic rearrangements, some of which can lead to cellular transformation. This error-prone repair pathway is triggered upon telomere de-protection to promote the formation of deleterious chromosome end-to-end fusions. Using next-generatio...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature14157

    authors： Mateos-Gomez PA,Gong F,Nair N,Miller KM,Lazzerini-Denchi E,Sfeir A

    更新日期：2015-02-12 00:00:00

  • Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer.

    abstract：:Replication of multipotential stem cells in long-term murine bone marrow cell culture is known to depend on the development of an adherent stromal cell layer. In these conditions, restricted haematopoietic progenitor cells have also been generated for up to several months1-3. However, maturation is observed only in th...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/305625a0

    authors： Wendling F,Shreeve MM,McLeod DL,Axelrad AA

    更新日期：1983-10-13 00:00:00

  • Dynamical transition of myoglobin revealed by inelastic neutron scattering.

    abstract：:Structural fluctuations in proteins on the picosecond timescale have been studied in considerable detail by theoretical methods such as molecular dynamics simulation, but there exist very few experimental data with which to test the conclusions. We have used the technique of inelastic neutron scattering to investigate...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/337754a0

    authors： Doster W,Cusack S,Petry W

    更新日期：1989-02-23 00:00:00

  • Competing memories of mitogen and p53 signalling control cell-cycle entry.

    abstract：:Regulation of cell proliferation is necessary for immune responses, tissue repair, and upkeep of organ function to maintain human health. When proliferating cells complete mitosis, a fraction of newly born daughter cells immediately enter the next cell cycle, while the remaining cells in the same population exit to a ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature23880

    authors： Yang HW,Chung M,Kudo T,Meyer T

    更新日期：2017-09-21 00:00:00

  • The oyster genome reveals stress adaptation and complexity of shell formation.

    abstract：:The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteom...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature11413

    authors： Zhang G,Fang X,Guo X,Li L,Luo R,Xu F,Yang P,Zhang L,Wang X,Qi H,Xiong Z,Que H,Xie Y,Holland PW,Paps J,Zhu Y,Wu F,Chen Y,Wang J,Peng C,Meng J,Yang L,Liu J,Wen B,Zhang N,Huang Z,Zhu Q,Feng Y,Mount A

    更新日期：2012-10-04 00:00:00

  • High resolution structure of the RNA duplex [U(U-A)6A]2.

    abstract：:RNA is involved in many biological functions, ranging from information storage and transfer to the catalysis of reactions involving both nucleic acids and proteins. Previous crystallographic studies on RNA oligomeric chains provide only averaged structures or information limited in resolution. The oligomer [U(U-A)6A]2...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/335375a0

    authors： Dock-Bregeon AC,Chevrier B,Podjarny A,Moras D,deBear JS,Gough GR,Gilham PT,Johnson JE

    更新日期：1988-09-22 00:00:00

  • Three-dimensional structure of the E. coli DNA-binding protein FIS.

    abstract：:The factor for inversion stimulation, FIS, is involved in several cellular processes, including site-specific recombination and transcriptional activation. In the reactions catalysed by the DNA invertases Gin, Hin and Cin, FIS stimulates recombination by binding to an enhancer sequence. Within the enhancer, two FIS di...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/349178a0

    authors： Kostrewa D,Granzin J,Koch C,Choe HW,Raghunathan S,Wolf W,Labahn J,Kahmann R,Saenger W

    更新日期：1991-01-10 00:00:00

  • FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes.

    abstract：:Mutations in the transcription factor FOXA1 define a unique subset of prostate cancers but the functional consequences of these mutations and whether they confer gain or loss of function is unknown1-9. Here, by annotating the landscape of FOXA1 mutations from 3,086 human prostate cancers, we define two hotspots in the...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-019-1318-9

    authors： Adams EJ,Karthaus WR,Hoover E,Liu D,Gruet A,Zhang Z,Cho H,DiLoreto R,Chhangawala S,Liu Y,Watson PA,Davicioni E,Sboner A,Barbieri CE,Bose R,Leslie CS,Sawyers CL

    更新日期：2019-07-01 00:00:00

  • Undermethylation associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid.

    abstract：:Genetic models predict that genomic rearrangement in hybrids can facilitate reproductive isolation and the formation of new species by preventing gene flow between the parent species and hybrid (sunflowers are an example). The mechanism underlying hybridization-induced chromosome remodelling is as yet unknown, althoug...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/29985

    authors： O'Neill RJ,O'Neill MJ,Graves JA

    更新日期：1998-05-07 00:00:00

  • The ecological cost of sex.

    abstract：:Why sex prevails in nature remains one of the great puzzles of evolution. Sexual reproduction has an immediate cost relative to asexual reproduction, as males only express their contribution to population growth through females. With no males to sustain, an asexual mutant can double its relative representation in the ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35005078

    authors： Doncaster CP,Pound GE,Cox SJ

    更新日期：2000-03-16 00:00:00

  • A stable silicon-based allene analogue with a formally sp-hybridized silicon atom.

    abstract：:Carbon chemistry exhibits a rich variety in bonding patterns, with homo- or heteronuclear multiple bonds involving sp-hybridized carbon atoms as found in molecules such as acetylenes, nitriles, allenes and carbon dioxide. Carbon's heavier homologues in group 14 of the periodic table--including silicon, germanium and t...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature01380

    authors： Ishida S,Iwamoto T,Kabuto C,Kira M

    更新日期：2003-02-13 00:00:00

  • Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9.

    abstract：:Angiosperms developed floral nectaries that reward pollinating insects. Although nectar function and composition have been characterized, the mechanism of nectar secretion has remained unclear. Here we identify SWEET9 as a nectary-specific sugar transporter in three eudicot species: Arabidopsis thaliana, Brassica rapa...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature13082

    authors： Lin IW,Sosso D,Chen LQ,Gase K,Kim SG,Kessler D,Klinkenberg PM,Gorder MK,Hou BH,Qu XQ,Carter CJ,Baldwin IT,Frommer WB

    更新日期：2014-04-24 00:00:00

  • Origin of Thy-1+ dendritic epidermal cells of adult mice from fetal thymic precursors.

    abstract：:The skin of mice contains dendritic epidermal cells carrying the Thy-1 antigen (Thy-1+ dEC) which express antigen receptors composed of the T-cell antigen receptor (TCR) gamma- and delta-chains. Although the role of the thymus in the generation of most T cells is well established, the involvement of the thymus in the ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/344068a0

    authors： Havran WL,Allison JP

    更新日期：1990-03-01 00:00:00

  • The background brightness of the milky way near 2500 A between lparallel= 72 degrees and 126 degrees.

    abstract：:Data from a rocket experiment show details which were not known from ground-based observations. ...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/226526a0

    authors： Sudbury GC,Ingham MF

    更新日期：1970-05-09 00:00:00

  • Origin of the orbital architecture of the giant planets of the Solar System.

    abstract：:Planetary formation theories suggest that the giant planets formed on circular and coplanar orbits. The eccentricities of Jupiter, Saturn and Uranus, however, reach values of 6 per cent, 9 per cent and 8 per cent, respectively. In addition, the inclinations of the orbital planes of Saturn, Uranus and Neptune take maxi...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/nature03539

    authors： Tsiganis K,Gomes R,Morbidelli A,Levison HF

    更新日期：2005-05-26 00:00:00

  • The emergence of transcriptional identity in somatosensory neurons.

    abstract：:More than twelve morphologically and physiologically distinct subtypes of primary somatosensory neuron report salient features of our internal and external environments1-4. It is unclear how specialized gene expression programs emerge during development to endow these subtypes with their unique properties. To assess t...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/s41586-019-1900-1

    authors： Sharma N,Flaherty K,Lezgiyeva K,Wagner DE,Klein AM,Ginty DD

    更新日期：2020-01-01 00:00:00

  • Osmotic generation of 'anomalous' fluid pressures in geological environments

    abstract：:Osmotic pressures are generated by differences in chemical potential of a solution across a membrane. But whether osmosis can have a significant effect on the pressure of fluids in geological environments has been controversial, because the membrane properties of geological media are poorly understood. 'Anomalous' pre...

    journal_title：Nature

    pub_type： 杂志文章

    doi：10.1038/35003174

    authors： Neuzil CE

    更新日期：2000-01-13 00:00:00