Abstract：
:Southern blot analyses using reduced stringency hybridization conditions have been employed to search for sequence homologies between rodent VL30 genes and murine leukemia virus (MuLV) proviruses. These constitute two classes of transposon-like elements previously believed to be genetically unrelated. Our results demonstrate that cloned representatives of both ecotropic and xenotropic-like proviruses share discrete regions of sequence homology with VL30 genes of both rat and mouse origin. These regions of homology exist in both 3' and 5' halves of the MuLV genome but do not include extensive portions of the long terminal repeat (LTR) or a 0.4 Kbp segment of the env gene specific for recently acquired ecotropic-type MuLV proviruses. DNA sequencing, however, revealed that the short inverted terminal repeat sequence of MuLV proviral LTRs is almost perfectly conserved at the terminus of an integrated mouse VL30 gene. These results suggest that recombination events with rodent VL30-type sequences occurred during early MuLV evolution. The strong conservation of the inverted terminal repeat sequence may reflect a common integration mechanism for VL30 elements and MuLV proviruses.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Giri CP,Hodgson CP,Elder PK,Courtney MG,Getz MJdoi
10.1093/nar/11.2.305subject
Has Abstractpub_date
1983-01-25 00:00:00pages
305-19issue
2eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract：:An improved algorithm was elaborated to analyse tRNA interaction with aminoacyl-tRNA synthetase based on analysis of tRNA sequences. The fundamental element defining the interaction between the tRNA and the synthetase is not a single nucleotide but a nucleotide combination named a tile which comprises of a given nucle...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.8.1941
更新日期：1993-04-25 00:00:00
abstract：:Dlx2, Lymphoid Enhancer Factor (Lef-1) and Msx2 transcription factors are required for several developmental processes. To understand the control of gene expression by these factors, chromatin immunoprecipitation (ChIP) assays identified Msx2 as a downstream target of Dlx2 and Lef-1. Dlx2 activates the Msx2 promoter i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl689
更新日期：2006-01-01 00:00:00
abstract：:Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks927
更新日期：2012-12-01 00:00:00
abstract：:AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku341
更新日期：2014-06-01 00:00:00
abstract：:RNA interference represents a potent intervention for cancer treatment but requires a robust delivery agent for transporting gene-modulating molecules, such as small interfering RNAs (siRNAs). Although numerous molecular approaches for siRNA delivery are adequate in vitro, delivery to therapeutic targets in vivo is li...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz900
更新日期：2019-12-02 00:00:00
abstract：:We present the PATHOgenic YEAst Search for Transcriptional Regulators And Consensus Tracking (PathoYeastract - http://pathoyeastract.org) database, a tool for the analysis and prediction of transcription regulatory associations at the gene and genomic levels in the pathogenic yeasts Candida albicans and C. glabrata Up...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw817
更新日期：2017-01-04 00:00:00
abstract：:Nuclear DNA binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein Oct-1. It binds to octamer related sequences from the enhancer of human papillomavirus type 18. The activity and intracellular distribution of p92 is regulated by ext...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.20.5725
更新日期：1991-10-25 00:00:00
abstract：:Various antitumor drugs stabilize DNA topoisomerase II-DNA transient covalent complexes. The complexes distribution along pBR322 DNA was shown previously to depend upon the nature of the drug (Tewey et al. (1984) Science 226, 466-468). The position in pBR322 of DNA cleavage by calf DNA topoisomerase II for 115 such si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.2861
更新日期：1991-06-11 00:00:00
abstract：:Many mutations occur as a result of DNA synthesis past the site of DNA damage by DNA damage bypass polymerases. The frequency and types of mutations not only depend on the nature of the damage, but also on the sequence context, as revealed from analysis of mutation spectra of DNA exposed to mutagens. Herein we report ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn595
更新日期：2008-10-01 00:00:00
abstract：:Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku303
更新日期：2014-06-01 00:00:00
abstract：:Homologous recombination (HR) is important in repairing errors of replication and other forms of DNA damage. In mammalian cells, potential templates include the homologous chromosome, and after DNA replication, the sister chromatid. Previous work has shown that the mammalian recombination machinery is organized to sup...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh280
更新日期：2004-02-20 00:00:00
abstract：:Well defined biomacromolecular patterns such as binding sites, catalytic sites, specific protein or nucleic acid sequences, etc. precisely modulate many important biological phenomena. We introduce PatternQuery, a web-based application designed for detection and fast extraction of such patterns. The application uses a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv561
更新日期：2015-07-01 00:00:00
abstract：:The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been proposed as regulators, but a potential role of the nuclear ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky368
更新日期：2018-07-06 00:00:00
abstract：:A new procedure for the purification of B. subtilis RNA polymerase, based on mild lysis of cells, low speed centrifugation, gel filtration, DEAE-Sephadex chromatography and affinity chromatography on DNA-cellulose, yields three forms of enzyme referred here as enzyme A, B and C. As revealed by SDS gel electrophoresis,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.3.603
更新日期：1977-03-01 00:00:00
abstract：:The three CD3 genes on human chromosome 11q23 encode proteins (gamma, delta and epsilon) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.3.459
更新日期：1990-02-11 00:00:00
abstract：:A number of different protecting groups were compared with respect to their usefulness for protection of 2'-hydroxyl functions during synthesis of oligoribonucleotides using the H-phosphonate approach. The comparison was between the t-butyldimethylsilyl (t-BDMSi), the o-chlorobenzoyl (o-CIBz), the tetrahydropyranyl (T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.1.94
更新日期：1994-01-11 00:00:00
abstract：:Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl772
更新日期：2006-01-01 00:00:00
abstract：:YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh889
更新日期：2004-10-19 00:00:00
abstract：:We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.9.3671
更新日期：1988-05-11 00:00:00
abstract：:Cells internalized synthetic oligonucleotides (oligos) in culture. The hybridization of these molecules to target RNA in the living cell was subsequently detected and characterized after fixation of the cells, with or without previous detergent extraction. Hybridized oligo was distinguished from free oligo in the cell...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.24.4946
更新日期：1995-12-25 00:00:00
abstract：:The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr914
更新日期：2012-01-01 00:00:00
abstract：:Numerous eukaryotic genes are alternatively spliced. Recently, deep transcriptome sequencing has skyrocketed proportion of alternatively spliced genes; over 95% human multi-exon genes are alternatively spliced. One fundamental question is: are all these alternative splicing (AS) events functional? To look into this is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1253
更新日期：2014-12-16 00:00:00
abstract：:High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.24.5051
更新日期：1996-12-15 00:00:00
abstract：:The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.12.2105
更新日期：1995-06-25 00:00:00
abstract：:In Saccharomyces cerevisiae, the DNA damage response (DDR) is activated by the spatio-temporal colocalization of Mec1-Ddc2 kinase and the 9-1-1 clamp. In the absence of direct means to monitor Mec1 kinase activation in vivo, activation of the checkpoint kinase Rad53 has been taken as a proxy for DDR activation. Here, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1222
更新日期：2010-04-01 00:00:00
abstract：:The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1112
更新日期：2018-01-04 00:00:00
abstract：:DNA topoisomerases and DNA site-specific recombinases are biologically important enzymes involved in a diverse set of cellular processes. We show that replacement of a phosphodiester linkage by a 5'-bridging phosphorothioate linkage creates an efficient suicide substrate for calf thymus topoisomerase I and lambda inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.2973
更新日期：1995-08-11 00:00:00
abstract：:The identification of genes with specific patterns of change (e.g. down-regulated and methylated) as phenotype drivers or samples with similar profiles for a given gene set as drivers of clinical outcome, requires the integration of several genomic data types for which an 'integrate by intersection' (IBI) approach is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1503
更新日期：2016-04-20 00:00:00
abstract：:Transcription factors (TFs) regulate gene expression by binding to short DNA sequence motifs, yet their binding specificities alone cannot explain how certain TFs drive a diversity of biological processes. In order to investigate the factors that control the functions of the pleiotropic TF STAT3, we studied its genome...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1300
更新日期：2013-02-01 00:00:00
abstract：:The initial genomic response to serum growth factors is the transcriptional activation of a set of immediate-early genes. Serum-induced transcriptional activation of several of these genes involves the formation of a ternary complex that includes the serum response factor (SRF), a 62 kDa ternary complex factor (TCF) a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.7.1345
更新日期：1996-04-01 00:00:00
