Abstract:
:Two virus-specific RNA species of 7.5 and 7.0 kilobases have been identified in avian myeloblastosis virus (AMV) by denaturing gel electrophoresis and blot hybridization analysis, and they were found to be in a 10:1 ratio. The individual RNAs direccted the cell-free synthesis of the 76,000-dalton "gag" protein and the 180,000-dalton "gal-pol" protein, thereby demonstrating 5' sequence homology of approximately 4.9 kilobases between the two species. Synthesis of these two precursor proteins by the AMV genome indicates structural differences between AMV and other avian acute leukemia viruses. The two viral RNAs were transcribed into complete cDNA copies with AMV DNA polymerase. Linear proviruses were found to be 90--100% resistant to S1 nuclease. Analysis of single-stranded transcripts demonstrated two distinct species of 2.6 and 2.3 x 10(6) daltons, and analysis of duplexes formed from the single-stranded transcripts demonstrated species of 5.2 and 4.0 x 10(6) daltons.
journal_name
Proc Natl Acad Sci U S Aauthors
Schulz RA,Chirikjian JG,Papas TSdoi
10.1073/pnas.78.4.2057subject
Has Abstractpub_date
1981-04-01 00:00:00pages
2057-61issue
4eissn
0027-8424issn
1091-6490journal_volume
78pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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