Abstract:
:The polypeptide core of mucin glycoprotein subunits resists cleavage by proteases. To determine if the carbohydrate side chains of these subunits protect the underlying polypeptide core from proteolytic cleavage, we compared the effect of pancreatic proteases on hog gastric mucin before and after cleavage of its carbohydrate moieties by bacterial glycosidases from an anaerobic human fecal culture supernate. Hog gastric mucin was resistant to pancreatic proteases: less than 10% of the mucin protein became soluble in 80% vol/vol ethanol after 24-h incubation with trypsin, alpha-chymotrypsin, and elastase, and its elution pattern from Sephadex G-200 remained unchanged after elastase treatment, with 90% eluting at the void volume. By contrast, after removal of 50% of its carbohydrates, mucin was susceptible to pancreatic proteases: 50% of mucin protein became soluble in 80% vol/vol ethanol after 24-h incubation with alpha-chymotrypsin and elastase, and 24% of mucin protein became soluble in 80% vol/vol ethanol after 24-h incubation with trypsin; after elastase treatment, its elution from Sephadex G-200 was markedly retarded. We conclude that the carbohydrate side chains of hog gastric mucin glycoprotein protect the underlying polypeptide core from proteolysis and that degradation of the carbohydrate side chains by glycosidases from fecal bacteria renders the polypeptide core susceptible to pancreatic proteases.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Variyam EP,Hoskins LCsubject
Has Abstractpub_date
1983-03-01 00:00:00pages
533-7issue
3eissn
0016-5085issn
1528-0012pii
S001650858300044Xjournal_volume
84pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Mutational inactivation of adenomatous polyposis coli (APC) is an early event in colorectal cancer (CRC) progression that affects the stability and increases the activity of β-catenin, a mediator of Wnt signaling. Progression of CRC also involves inactivation of signaling via transforming growth facto...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.11.026
更新日期:2012-03-01 00:00:00
abstract::Non-major histocompatibility complex-restricted cytotoxicity triggered by antibodies to the CD3 component of the human T-cell receptor complex is thought to be an indirect measure of in vivo primed cytotoxic T-cell activity. We have used this technique to examine the lytic activity of freshly isolated T cells from non...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90554-9
更新日期:1988-04-01 00:00:00
abstract:DESCRIPTION:Thanks to ready access to hydrogen breath testing, small intestinal bacterial overgrowth (SIBO) is now commonly diagnosed among individuals presenting with a variety of gastrointestinal and even nongastrointestinal symptoms and is increasingly implicated in lay press and media in the causation of a diverse ...
journal_title:Gastroenterology
pub_type: 实务指引
doi:10.1053/j.gastro.2020.06.090
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND & AIMS:Hereditary hemochromatosis is a common disorder of iron homeostasis characterized by increased dietary iron absorption and progressive iron accumulation, mainly in the liver. Most patients are homozygous for the C282Y mutation in the HFE gene. However, not all individuals carrying the hemochromatosis-...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2004.01.021
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND & AIMS:Changes in the properties of visceral sensory neurons contribute to the development of gastrointestinal pain. However, little is known about the molecules involved in mechanosensation from the gastrointestinal tract. We investigated the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.01.031
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND & AIMS:Body weight (BW) loss and reduction of blood ghrelin level are commonly observed after total gastrectomy (TG). A prospective study was designed to elucidate whether exogenous ghrelin administration prevents postoperative BW loss by improving appetite and oral food intake in patients with gastric cance...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2009.12.058
更新日期:2010-04-01 00:00:00
abstract::Absorptive cells of the small intestine are highly polarized cells with distinct microvillus membrane (MVM) and basolateral plasma membrane domains. We compared membrane structure in the following preparations of rat small intestine commonly used for in vitro study of MVM function: epithelial sheets, isolated epitheli...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90194-0
更新日期:1986-12-01 00:00:00
abstract:BACKGROUND & AIMS:Fecal microbiota transplantation (FMT) is a highly effective therapy for recurrent Clostridium difficile infection (CDI). However, transferring undefined living bacteria entails uncontrollable risks for infectious and metabolic or malignant diseases, particularly in immunocompromised patients. We inve...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2016.11.010
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND & AIMS:Sjögren's syndrome and autoimmune pancreatitis are disorders with decreased function of salivary, lacrimal glands, and the exocrine pancreas. Nonobese diabetic/ShiLTJ mice and mice transduced with the cytokine BMP6 develop Sjögren's syndrome and chronic pancreatitis and MRL/Mp mice are models of autoi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.06.011
更新日期:2017-10-01 00:00:00
abstract::We have developed an isolated intestinal cell system to study the regulation of cholecystokinin release. Enzymatically dispersed canine jejunal mucosal cells were separated by counterflow elutriation to enrich cholecystokinin content 20-fold. Release of cholecystokinin from freshly isolated cells was determined by rad...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90632-3
更新日期:1986-09-01 00:00:00
abstract:BACKGROUND & AIMS:Outcomes of liver transplantation for hepatocellular carcinoma (HCC) are determined by cancer-related and non-related events. Treatments for hepatitis C virus infection have reduced non-cancer events among patients receiving liver transplants, so reducing HCC-related death might be an actionable end p...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.09.025
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND & AIMS:Abnormal processing of visceral sensation at the level of the central nervous system is believed to be involved in functional dyspepsia. However, compared with studies of stimulation-related changes in brain activity, few studies have focused on resting brain activity, which also is important in patho...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.05.003
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND & AIMS:Gastroesophageal reflux disease (GERD) interferes with the quality of life and carries an increased risk for esophageal adenocarcinoma. We investigated genetic influence in the development of reflux. METHODS:We compared concordance for reflux in monozygotic (MZ) and dizygotic (DZ) twins. All twins ag...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.30301
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND & AIMS:Gain-of-function mutations in the KIT receptor tyrosine kinase gene and rare mutations in the platelet-derived growth factor receptor alpha (PDGFRA) gene are important events in gastrointestinal stromal tumor (GIST) development. Different mutations are reportedly associated with distinctive phenotypes...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.01.043
更新日期:2006-05-01 00:00:00
abstract::Endosonography was performed preoperatively in 33 patients with common bile duct carcinoma and in 43 patients with carcinoma of the common hepatic duct and its bifurcation. The results were correlated with the histology of resected specimens according to the new (1987) TNM (tumor, node, metastasis) classification. End...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Effects of opiates on intestinal motor activity and transport of water and electrolytes have been studied separately in previous investigations. The aim of these experiments was to evaluate simultaneously the effects of a synthetic opiate, loperamide, on motor activity and transport in the human intestine. Jejunal, il...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1016/0016-5085(86)90079-x
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND & AIMS:In patients with chronic hepatitis C who failed to respond to standard therapy, high-dose pegylated interferon (IFN)-α and/or ribavirin could induce a stronger antiviral response and prevent treatment failure and HCV resistance when combined with direct-acting antivirals. The influence of genetic dete...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2011.03.039
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND & AIMS:There is controversy over the optimal management strategy for patients with acute pancreatitis (AP). Studies have shown a hospital volume benefit for in-hospital mortality after surgery, and we examined whether a similar mortality benefit exists for patients admitted with AP. METHODS:Using the Nation...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.08.056
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND & AIMS:There are intra- and interobserver variations in endoscopic assessment of ulcerative colitis (UC) and biopsies are often collected for histologic evaluation. We sought to develop a deep neural network system for consistent, objective, and real-time analysis of endoscopic images from patients with UC. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.02.012
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND & AIMS:Liver disease has been associated with cardiovascular disorders, but little is known about the relationship between serum levels of alanine aminotransferase (ALT) and markers of atherogenesis. We investigated the relationship between low-normal and high-normal levels of ALT and an extended panel of ca...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2013.08.036
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND & AIMS:The finding of bone morphogenetic protein (BMP) receptor 1a mutations in juvenile polyposis suggests that BMPs are important in colorectal cancer (CRC). We investigated the BMP pathway in sporadic CRC. METHODS:We investigated BMP receptor (BMPR) expression using immunoblotting and sequenced BMPR2 in ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.02.059
更新日期:2008-05-01 00:00:00
abstract::The efficacy of various adsorbents for endotoxin was tested in vitro and in vivo using a murine experimental model of gut-derived endotoxemia. A quantitative limulus amebocyte lysate microtiter test and the limulus amebocyte lysate tube test were used to determine intestinal and circulating levels of endotoxin. Kaopec...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND & AIMS:Canalicular secretion of bile acids is stimulated by tauroursodesoxycholate (TUDC). This study investigates the underlying mechanisms. METHODS:TUDC effects on mitogen-activated protein (MAP) kinases, taurocholate (TC) excretion, proteolysis, and the localization of the bile salt export pump (Bsep) we...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.26262
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND & AIMS:Recently, the immediate early gene h-sgk was cloned as a hypertonicity-induced gene from human hepatoma cells. The aim of this study was to localize h-sgk messenger RNA (mRNA) expression in normal and inflamed intestinal mucosa and to identify potential transcriptional regulators. METHODS:h-sgk mRNA ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70011-9
更新日期:1999-05-01 00:00:00
abstract::Uptake of inorganic sulfate into brush-border membrane vesicles isolated by a calcium precipitation method from rat small intestine was investigated using a rapid filtration technique and 35Sulfur acid as tracer. Sulfate uptake by membrane vesicles was osmotically sensitive, suggesting transport into an intravesicular...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Initially, scintigraphy was established as a valid method for detecting and quantitating enterogastric reflux. A new, tubeless technique for the measurement of enterogastric reflux was developed. 99mTc bound to [(2,6 dimethylphenylcarbamoylmethyl) iminodiacetic acid] (5 mCi) was administered intravenously to visualize...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::To examine the premise that exercise reduces the gastrointestinal transit time, we evaluated the effect of walking 4.5 km in an hour on mouth-to-cecum transit time. Twenty-three healthy volunteers, 9 men and 14 women, with an age range of 19-28 yr, were studied. After an overnight fast, the subjects ingested 10 g of l...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-03-01 00:00:00
abstract::One of the least anticipated and less heralded outcomes of mouse genetics has been to rediscover whole organism physiology. Among the many unexpected findings that it has brought to our attention has been the realization that gut-derived serotonin is a hormone-inhibiting bone formation. The importance of this discover...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2011.06.011
更新日期:2011-08-01 00:00:00
abstract::For decades, interactions between the enteric neuromuscular apparatus and the central nervous system have served as the primary focus of pathophysiological research in the functional gastrointestinal disorders. The accumulation of patient reports, as well as clinical observations, has belatedly led to an interest in t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2016.02.028
更新日期:2016-02-18 00:00:00
abstract:BACKGROUND & AIMS:The adenoma detection rate (ADR) is an important quality indicator of screening colonoscopy; it is inversely associated with risk of interval cancers and colorectal cancer mortality. We assessed trends in the ADR in the first 10 years of the German screening colonoscopy program. METHODS:We calculated...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.04.012
更新日期:2015-08-01 00:00:00