Abstract：
:The classic histamine release test involved washing and isolating of leukocytes from heparinized whole blood. Siraganian automatized the extraction procedure, working initially with isolated leukocytes in Tris-human serum albumin-Ca++Mg++ buffer, with optimal results. One year later, he described a continuous flow system that permitted the study of whole blood from atopic patients. This method was easier to perform since the isolation of the white blood cells is no longer necessary. On the other hand, the results working with whole blood seem to be better than with isolated leukocytes. We have published modifications of the continuous flow technique, working also with whole blood. Our results agree with those of Siraganian and others. An alternative to working with whole blood or isolated cells in synthetic buffers could be the use of autologous diluted plasma. In this way, the cells are centrifuged and the plasma poured off. The plasma can be used for other determinations, such as RIA, ELISA, etc. The blood is reconstituted to its original volume with a PBS-Ca++Mg++ buffer and processed as described. The results using the whole blood and "washed cells" were very similar. But in some cases we found positive histamine release in "washed leukocytes" but not in whole blood. This striking phenomenon can be explained by the presence in plasma of blocking non-IgE antibodies. If this is the case, the removal of approximately 85% of the plasma will also lead to the disappearance of the 85% of these blocking antibodies. On the other hand, all the positive results obtained using whole blood have been confirmed using "washed cells". Therefore, the use of "washed cells" has several advantages with respect to whole blood assay or leukocyte isolation.
journal_name
Allergol Immunopathol (Madr)journal_title
Allergologia et immunopathologiaauthors
Zabay JM,Moneo I,Bootello Asubject
Has Abstractpub_date
1982-05-01 00:00:00pages
195-200issue
3eissn
0301-0546issn
1578-1267journal_volume
10pub_type
杂志文章abstract：:Atopic dermatitis (AD) is a multifaceted disease that involves a complex interplay between the skin and the immune system. The course of the disease depends strongly on the genetic background of the patient and on yet poorly-defined environmental factors. Changes in lifestyle could be behind the dramatic rise in the p...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章,评审
doi：10.1016/j.aller.2017.07.002
更新日期：2018-01-01 00:00:00
abstract：:The presence of autoantibodies in 125 patients with cryptogenic chronic hepatitis (CH) was assessed by indirect immunofluorescence (IFL) on standard tissue sections and on Hep-2 cells. Smooth muscle antibodies (SMA) were found in a high percentage of patients without any difference between the anti-HBc positive and th...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:We report the cases of 2 patients diagnosed as having primula dermatitis. Both patients were housewives who had been exposed to primula plants. Although only one related her clinical manifestations with looking after plants, the symptoms of both remitted when contact with primula was avoided. The diagnosis was carried...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Serum zinc, copper and iron levels together with content of zinc in hair were investigated in 13 patients with common variable immunodeficiency (CVID) and in 13 controls. A significant decrease in serum zinc (mean = 11.3 mumol/l, SD = 2.9 in CVID patients compared to mean 14.3 mumol/l, SD = 2.4 in controls) and iron l...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：INTRODUCTION:Asthma is an inflammatory disorder of the airways associated with bronchial hyperresponsiveness, airway obstruction, and increased mucus production, with a predominance of type 2 immune response (Th2). According to the hygiene hypothesis, exposure to environmental bacterial lipopolysaccharide (LPS) may ind...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2014.10.005
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:sublingual-swallow immunotherapy has been recognized as safe and effective in adults. Different treatment schedules have been proposed and used, but the current experience in children is insufficient. We carried out a study to evaluate the clinical efficacy and the safety in children allergic to grass pollen...
journal_title：Allergologia et immunopathologia
pub_type： 临床试验,杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:Associations between liver function and serum IgE levels have recently been observed in children. However, the relationship in adults is unclear. Therefore, it was aimed to study associations of liver function and serum total and food-specific IgE concentrations in a national and population-based study. MET...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2014.02.009
更新日期：2015-07-01 00:00:00
abstract：:Chronic bronchitis is a disease related to numerous etiologic factors: infections, climate influence, air pollution, cigarette smoking, etc. From a pathogenetic point of view, chronic bronchitis is generally considered as the phlogistic resultant of various irritative conditions, with a characteristic neutrophil compo...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：BACKGROUND:Asthma is an important childhood disease. Recent surveys of the International Study of Asthma and Allergies in Childhood (ISAAC) suggest that the prevalence of asthma is increasing but these surveys do not include any pulmonary tests to confirm the possible diagnosis of asthma. OBJECTIVE:To compare bronchod...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/s0301-0546(04)79265-4
更新日期：2004-11-01 00:00:00
abstract：:It is suggested that increased reactivity in asthma is not limited to the bronchi but also exists in the mediator releasing system. We have previously observed that the basophils of atopic asthmatics are degranulated by polymyxin B in vitro. The present study attempts to relate this basophil degranulation to atopy. Th...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:For one year dust was sampled monthly from a single mattress. Until August mite counts were at background levels. In September and October there was a burst in the counts for the house-dust mite, Dermatophagoides farinae and for the house mite, Glycyphagus domesticus. Their occurrence might be interpreted as a respons...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:We report a case of nasal candidiasis in an immunocompetent host who denied the use of topical or systemic corticosteroids. The patient was treated with itraconazole 100 mg. with resolution of the symptoms. ...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:Although studies have reported an association between air pollutants and increased allergic airway diseases, such as allergic rhinitis and nasal polyposis, the underlying mechanisms are not fully understood. A limited number of studies have suggested that diesel exhaust particles (DEP) play a role in atopy a...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2016.10.015
更新日期：2017-01-01 00:00:00
abstract：:This work deals with a study of the changes observed throughout the four seasons of the year concerning the number of fungi which can be found in the atmosphere of Barcelona. The results obtained will bring us to the conclusion that during the autumn and winter months the number of conidia per m3 is greater than in sp...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：BACKGROUND:Allergic diseases are very common, with the estimated cumulative prevalence in Colombia for asthma and allergic rhinitis being 22.7% and 31.3% respectively, and with a clear upward trend. A major social as well as economic impact associated with allergic disease has been described. Because in Colombia there ...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2011.11.008
更新日期：2012-11-01 00:00:00
abstract：:A procedure is described for the cytochemical detection of esterase alpha naphtyl acetate (ANAE) and beta glucuronidase (BG) activity in the circulating lymphocytes of man. The number of BG positive cells (TBG) as evaluated on the smears of Ficoll Urovision purified cells, correlated well with the number of T gamma (E...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:Asthma morbidity and mortality has increased. One of the possible causes is the excessive use of beta agonists. The aim of this study is to compare the effects of six week treatment with beclomethasone alone (Ibec) or the combination of beclomethasone-salmeterol (Ibe + Isal) on serum potassium (K), CPK-MB and ECG in c...
journal_title：Allergologia et immunopathologia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0301-0546(01)79010-6
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Asthma is the most common chronic disease affecting children and adolescents (AD). We evaluated the prevalence of asthma and associated factors in adolescents living in a developing region in northeast Brazil using the ISAAC (International Study of Asthma and Allergies in Childhood) methodology. METHODS:Acc...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2014.05.006
更新日期：2015-09-01 00:00:00
abstract：:We present the clinical case of a 8-years-old boy suffering a fixed drug reaction attributed to the oral intake of loratadine. He is an atopic child with perennial rhinitis and asthma and marked hypersensitivity to the house-dust mite Dermatophagoides pteronyssinus who is receiving inhaled corticosteroids and b2-agoni...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/s0301-0546(03)79199-x
更新日期：2003-09-01 00:00:00
abstract：:A 34-year-old woman referred episodes of gastrointestinal disorders and sometimes angioedema of the lips which appeared within 2 hours from the ingestion of packed food like biscuits, cereals or fruit juice. A patch test with the standard European series and with food preservatives was positive for fragrances mix. The...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1157/13112995
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:Scarcity of reliable data on food allergy prevalence exists in Turkey. We aimed to assess reported and confirmed IgE-mediated food allergy prevalence, and define the spectrum of allergenic food. METHODS:We prospectively evaluated the ISAAC Phase II study population for food allergy. Participants that report...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章,多中心研究
doi：10.1016/j.aller.2012.05.005
更新日期：2013-11-01 00:00:00
abstract：:Since the first decades of the twentieth century, some authors have believed bacterial respiratory infection to be an important triggering factor in bronchial asthma, drawing attention to an asthmatic response to infection. In this context, already in 1995, we presented a study on nasal secretion cultures and the rela...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章,评审
doi：10.1016/s0301-0546(01)79035-0
更新日期：2001-05-01 00:00:00
abstract：:The mechanisms of action of prophylactic antiallergic drugs, especially disodium cromoglycate (DSCG), have been reviewed. Some evidence is presented that suggests that DSCG acts not only as an inhibitor of mediator release from mast cells, but also by inhibiting the effects of mediators on bronchial and intestinal smo...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章,评审
doi：
更新日期：1981-11-01 00:00:00
abstract：:We studied a group of asthmatic patients with aspirin intolerance who were RAST-negative for house-dust and Dermatophagoides-pteronyssinus despite having positive skin tests and positive inhalation tests to these allergens. Total haemolytic activity (CH50), C3, C4 and C3PA were measured after allergen-induced bronchos...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Thirty-four patients suffering from rhinoconjunctivitis with or without asthma due to grass pollen, were submitted to sublingual immunotherapy according to a double blind placebo controlled experimental plan; eighteen patients received the active therapy, sixteen the placebo. A rush preseasonal treatment schedule was ...
journal_title：Allergologia et immunopathologia
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：1995-09-01 00:00:00
abstract：:The spore concentrations of Alternaria, a genera of microfungi widely considered as allergenic by different authors (Caplin & Haynes, Gravesen, Gregory, Larsen, Petersen & Sandberg), have been investigated in the atmosphere of Córdoba (Spain) during the period of April, 1983 to March, 1985 by means of the volumetric (...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:The system that protects body from infectious agents is immune system. On occasions, the system seldom reacts with some foreign particles and causes allergy. Allergies of the ear, nose and throat (ENT) often have serious consequences, including impairment and emotional strain that lowers the quality of life of patient...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章,评审
doi：10.1016/j.aller.2016.10.010
更新日期：2017-01-01 00:00:00
abstract：:A total of 181 allergic patients were studied and classified according to the causal antigen, 92 patients were sensitized to the mixed grass pollen, 55 to house dust, 28 to Candida albicans, 31 patients with egg allergy and 25 with milk allergy. All of them presented strong positivity to anamnesis, intracutaneous test...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:The modulation by norepinephrine and serotonin of the persistent antibody response to autologous and heterologous erythrocytes has been studied in [(C27Bl6xCBA/Ca) F1] mice. Serotonin (subcutaneously administered) transiently decreased the response to both autologous erythrocytes and rat erythrocytes; nevertheless, in...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND:Diagnosing asthma in children is a challenge and using a single biomarker from exhaled breath condensate (EBC) showed the lack of improvement in it. OBJECTIVE:The aim of this study was to assess the diagnostic potential of a pattern of simple chemical biomarkers from EBC in diagnosing asthma in children in ...
journal_title：Allergologia et immunopathologia
pub_type： 杂志文章
doi：10.1016/j.aller.2016.05.001
更新日期：2017-01-01 00:00:00
