Abstract:
:To examine the influence of thyrotropin (TSH) on the thyroglobulin (Tgb) mRNA content, the latter was evaluated in the cytoplasm of hog thyroid cells cultured in the absence (control cells) or presence of TSH. The Tgb mRNA levels were determined by, (i) kinetics of hybridization to sheep Tgb cDNA, (ii) capacity of coding for peptides immunologically related to Tgb in reticulocyte lysate. In cells cultured for 4 days in the absence of TSH, the content of Tgb mRNA sequences decreased to 30% of its initial value and the messenger activity to 15%. Conversely, TSH maintained the initial Tgb mRNA level in cells cultured in its presence, and TSH concentrations 50 micronU/ml or 5 mU/ml gave identical results. At each period tested poly (A) content was the same in TSH-treated and control cells. When TSH was added to media after 4 or 8 days culture without TSH, the Tgb mRNA level was partially restored. These results suggest that TSH exerts a positive control on Tgb gene expression through modulation of Tgb mRNA content of thyroid cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chebath J,Chabaud O,Mauchamp Jdoi
10.1093/nar/6.10.3353subject
Has Abstractpub_date
1979-07-25 00:00:00pages
3353-67issue
10eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract::The transcriptional promoter of satellite 2 from the eastern newt, Notophthalmus viridescens, was analyzed by assaying the activity of deleted or mutated satellite 2 clones in Xenopus laevis oocytes. Two elements in the promoter were found to be important for transcription. These elements have sequences that are simil...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.22.4697
更新日期:1994-11-11 00:00:00
abstract::The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa479
更新日期:2020-07-09 00:00:00
abstract::The editing function of aminoacyl-tRNA synthetases (aaRSs) is indispensible for formation of the correct aminoacyl-tRNAs. Editing deficiency may lead to growth inhibition and the pathogenesis of various diseases. Herein, we confirmed that norvaline (Nva) but not isoleucine or valine is the major threat to the editing ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx487
更新日期:2017-07-07 00:00:00
abstract::We have isolated a rat cDNA, named FE65, hybridizing to an mRNA of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. An mRNA of the same size is present in human neuroblastoma cells and is absent from other human cell lines. The FE65 cDNA contains an ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5269
更新日期:1991-10-11 00:00:00
abstract::Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki919
更新日期:2005-10-24 00:00:00
abstract::The transactivator responsive region (TAR) present in the 5'-NTR of the HIV-1 genome represents a potential target for antiretroviral intervention and a model system for the development of specific inhibitors of RNA-protein interaction. Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA(TAR)) con...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki743
更新日期:2005-08-02 00:00:00
abstract::Plant viruses ubiquitously mediate the induction of miR168 trough the activities of viral suppressors of RNA silencing (VSRs) controlling the accumulation of ARGONAUTE1 (AGO1), one of the main components of RNA silencing based host defence system. Here we used a mutant Tombusvirus p19 VSR (p19-3M) disabled in its main...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt846
更新日期:2014-01-01 00:00:00
abstract::Polyploidy, the presence of multiple sets of chromosomes that are similar but not identical, complicates both chromosome walking and assembly of sequence-ready contigs for many plant taxa including a large number of economically-significant crops. Traditional 'dot-blot hybridization' or PCR-based assays for identifyin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.7.e23
更新日期:2000-04-01 00:00:00
abstract::Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library scree...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr609
更新日期:2011-10-01 00:00:00
abstract::Solution structures of DNA duplexes containing oxanine (Oxa, O) opposite a cytosine (O:C duplex) and opposite a thymine (O:T duplex) have been solved by the combined use of (1)H NMR and restrained molecular dynamics calculation. One mismatch pair was introduced into the center of the 11-mer duplex of [d(GTGACO(6)CACTG...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr872
更新日期:2012-02-01 00:00:00
abstract::Toxin-antitoxin systems are widespread in bacteria and archaea. They perform diverse functional roles, including the generation of persistence, maintenance of genetic loci and resistance to bacteriophages through abortive infection. Toxin-antitoxin systems have been divided into three types, depending on the nature of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks231
更新日期:2012-07-01 00:00:00
abstract::Nuclear DNA binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein Oct-1. It binds to octamer related sequences from the enhancer of human papillomavirus type 18. The activity and intracellular distribution of p92 is regulated by ext...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.20.5725
更新日期:1991-10-25 00:00:00
abstract::Activating sequence factor 1 (ASF-1) is a nuclear DNA-binding activity that is found in monocots and dicots. It interacts with several TGACG-containing elements that have been characterized from viral and T-DNA genes, the prototypes of which are the as-1 element of the CaMV 35S promoter and the ocs element from the oc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.18.3778
更新日期:1995-09-25 00:00:00
abstract::A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.7.2189
更新日期:1982-04-10 00:00:00
abstract::The activity of MHC class II promoters depends upon conserved regulatory signals one of which, the extended X-box, contains in its X2 subregion a sequence related to the cAMP response element, CRE and to the TPA response element, TRE. Accordingly, X2 is recognized by the AP-1 factor and by other c-Jun or c-Fos contain...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.8.1811
更新日期:1993-04-25 00:00:00
abstract::Restriction endonuclease Bse634I recognizes and cleaves the degenerate DNA sequence 5'-R/CCGGY-3' (R stands for A or G; Y for T or C, '/' indicates a cleavage position). Here, we report the crystal structures of the Bse634I R226A mutant complexed with cognate oligoduplexes containing ACCGGT and GCCGGC sites, respectiv...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks300
更新日期:2012-08-01 00:00:00
abstract::We have analyzed the molecular mechanism that makes translation of the MS2 replicase cistron dependent on the translation of the upstream coat cistron. Deletion mapping on cloned cDNA of the phage shows that the ribosomal binding site of the replicase cistron is masked by a long distance basepairing to an internal coa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.19.6955
更新日期:1985-10-11 00:00:00
abstract::Next-generation sequencing is excellently suited to evaluate the abundance of mRNAs to study gene expression. Here we compare two alternative technologies, cap analysis of gene expression (CAGE) and serial analysis of gene expression (SAGE), for the same RNA samples. Along with quantifying gene expression levels, CAGE...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq602
更新日期:2010-09-01 00:00:00
abstract::Exome sequencing strategy is promising for finding novel mutations of human monogenic disorders. However, pinpointing the casual mutation in a small number of samples is still a big challenge. Here, we propose a three-level filtration and prioritization framework to identify the casual mutation(s) in exome sequencing ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1257
更新日期:2012-04-01 00:00:00
abstract::Aminoglycosides antibiotics negate dissociation and recycling of the bacterial ribosome's subunits by binding to Helix 69 (H69) of 23S rRNA. The differential binding of various aminoglycosides to the chemically synthesized terminal domains of the Escherichia coli and human H69 has been characterized using spectroscopy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1253
更新日期:2010-05-01 00:00:00
abstract::The pac1+ gene of the fission yeast Schizosaccharomyces pombe is essential for viability and its overexpression induces sterility and suppresses mutations in the pat1+ and snm1+ genes. The pac1+ gene encodes a protein that is structurally similar to RNase III from Escherichia coli, but its normal function is unknown. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.12.2377
更新日期:1996-06-15 00:00:00
abstract::The TPA-inducible transcription factor AP-1, consisting of homo- or hetero-dimers of members of the Jun- and Fos-families, regulates transcription of a wide variety of genes containing the TPA response element (TRE). In P19 embryonal carcinoma (EC) cells, Jun D is the only component of AP-1 expressed, while in these c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.1.125
更新日期:1992-01-11 00:00:00
abstract::A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data, especially those obtained by the sequencing method using M13 phages. Programs NUCDAT and PROTEN are for analyzing DNA/RNA and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part1.101
更新日期:1984-01-11 00:00:00
abstract::Synthetic biology seeks to envision living cells as a matter of engineering. However, increasing evidence suggests that the genetic load imposed by the incorporation of synthetic devices in a living organism introduces a sort of unpredictability in the design process. As a result, individual part characterization is n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1280
更新日期:2016-01-08 00:00:00
abstract::DNA curvature plays a well-characterized role in many transcriptional regulation mechanisms. We present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. Genes with highly curved upstream regions were identified in orthologous groups, based o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg882
更新日期:2003-12-01 00:00:00
abstract::A new series of DNA bis-intercalators is reported in which acridine moieties are connected by rigid and extended pyridine-based linkers of varied length. Cross-linking of DNA by bis-intercalation is inferred from the unwinding and folding of linear DNA induced by the compounds; after ligation and removal of the bis-in...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.5.983
更新日期:1992-03-11 00:00:00
abstract::Cell specific expression of the insulin gene is achieved through transcriptional mechanisms operating on 5' flanking DNA elements. In the enhancer of rat I insulin gene, two elements, the Nir and Far boxes, located at positions -104 and -233 respectively and containing the same octameric motif are essential for B cell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.7.1595
更新日期:1993-04-11 00:00:00
abstract::A system for the tetracycline-inducible regulation of gene expression in mycobacteria has been developed. We have sub-cloned the tetRO region from the Corynebacterium glutamicum TetZ locus into a mycobacterial shuttle plasmid, making expression of genes cloned downstream of tetRO responsive to tetracycline. Using the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni023
更新日期:2005-02-01 00:00:00
abstract::Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (sha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx915
更新日期:2017-12-01 00:00:00
abstract::The phenotypic adjustments of Mycobacterium tuberculosis are commonly inferred from the analysis of transcript abundance. While mechanisms of transcriptional regulation have been extensively analysed in mycobacteria, little is known about mechanisms that shape the transcriptome by regulating RNA decay rates. The aim o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz251
更新日期:2019-06-20 00:00:00