当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Three types of gap junctions interconnecting intestinal epithelial cells visualized by freeze-etching.

Three types of gap junctions interconnecting intestinal epithelial cells visualized by freeze-etching.

Abstract：

:Gap junctions are specialized membrane regions that seem to mediate intercellular communication. They appear to contain closely packed arrays of equally sized particles all of which, upon freeze-cleaving, remain attached to one membrane leaflet and not to the other. One gap junction cleavage face, therefore, always exhibits a closely packed array of particles, while the other features a corresponding array of pits. By using these morphological criteria, we have been able to distinguish three different types of gap junctions interlinking adjacent epithelial cells of the small intestine. All three types may be found in close proximity to each other, and in all cases, the particles remain attached to the cleavage face of the cytoplasmic membrane leaflet (A-face). The most frequently encountered type-I gap junctions, which have already been observed in many other tissues, possess 8- to 9-nm (80- to 90-A) particles with a center-to-center spacing of 9-10 nm (90-100 A) when packed in a hexagonal lattice. Type-II gap junctions are always found in close association with type-I junctions. They can be distinguished from the type-I junctions by the greater size [10-11 nm (100-110 A) in diameter] and the greater spacing (190-200 A) of their hexagonally arrayed particles. In contrast, the particles of the type-III gap junctions are arranged in very small rectilinear arrays with a spacing of only 6-8 nm (60-80 A). Gap junctions may be involved in the control of intercellular flow of different types of regulatory molecules.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Staehelin LA

doi

10.1073/pnas.69.5.1318

subject

Has Abstract

pub_date

1972-05-01 00:00:00

pages

1318-21

issue

5

eissn

0027-8424

issn

1091-6490

journal_volume

69

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Evidence that the Y chromosome influences autoimmune disease in male and female mice.

    abstract：:Experimental allergic encephalomyelitis (EAE), an autoimmune model of multiple sclerosis, is a complex disease influenced by genetic, intrinsic, and environmental factors. In this study, we questioned whether parent-of-origin effects influence EAE, using reciprocal F2 intercross progeny generated between EAE-susceptib...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0600536103

    authors： Teuscher C,Noubade R,Spach K,McElvany B,Bunn JY,Fillmore PD,Zachary JF,Blankenhorn EP

    更新日期：2006-05-23 00:00:00

  • In vitro conversion of estradiol-receptor protein to its nuclear form: dependence on hormone and DNA.

    abstract：:Early events in the action of 17-beta-estradiol can be studied in soluble extracts of rat uterus by exposure of the estradiol-receptor protein to a DNA-cellulose matrix. After complexing with [(3)H]estradiol, the 4S receptor protein binds to the DNA, and it can be eluted with buffer of high ionic strength as a more ti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.8.2105

    authors： Yamamoto KR,Alberts BM

    更新日期：1972-08-01 00:00:00

  • Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants.

    abstract：:Polycomb-group (Pc-G) proteins ensure late maintenance of transcriptional repression outside the expression domain of target genes in flies and vertebrates. They act in complexes, presumably by modulating chromatin structure. In Drosophila, they have been found to be associated with transcriptionally inactive loci but...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2237046100

    authors： d Graaff W,Tomotsune D,Oosterveen T,Takihara Y,Koseki H,Deschamps J

    更新日期：2003-11-11 00:00:00

  • Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors.

    abstract：:PCR was used to isolate nucleotide sequences that may encode novel members of the neuropeptide Y receptor family. By use of a PCR product as a hybridization probe, a full-length human cDNA was isolated that encodes a 375-aa protein with a predicted membrane topology identifying it as a member of the G-protein-coupled ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.10.4661

    authors： Yan H,Yang J,Marasco J,Yamaguchi K,Brenner S,Collins F,Karbon W

    更新日期：1996-05-14 00:00:00

  • Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers.

    abstract：:We have made direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture. AChR were fluorescently marked with tetramethylrhodamine-labeled alpha-bungarotoxin and AChR lateral motion was measured by the fluore...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.12.4594

    authors： Axelrod D,Ravdin P,Koppel DE,Schlessinger J,Webb WW,Elson EL,Podleski TR

    更新日期：1976-12-01 00:00:00

  • Universal behavior of the osmotically compressed cell and its analogy to the colloidal glass transition.

    abstract：:Mechanical robustness of the cell under different modes of stress and deformation is essential to its survival and function. Under tension, mechanical rigidity is provided by the cytoskeletal network; with increasing stress, this network stiffens, providing increased resistance to deformation. However, a cell must als...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0901462106

    authors： Zhou EH,Trepat X,Park CY,Lenormand G,Oliver MN,Mijailovich SM,Hardin C,Weitz DA,Butler JP,Fredberg JJ

    更新日期：2009-06-30 00:00:00

  • Oncogenic transformation by the tax gene of human T-cell leukemia virus type I in vitro.

    abstract：:Human T-cell leukemia virus type I (HTLV-I) is a causative agent of adult T-cell leukemia (ATL). To elucidate the role of HTLV-I in leukemogenesis, we examined the biological activity of a defective HTLV-I provirus with the env-pX 3' long terminal repeat region cloned from leukemic cells of an ATL patient. Transfectio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.3.1071

    authors： Tanaka A,Takahashi C,Yamaoka S,Nosaka T,Maki M,Hatanaka M

    更新日期：1990-02-01 00:00:00

  • Gallstones play a significant role in Salmonella spp. gallbladder colonization and carriage.

    abstract：:Salmonella enterica serovar Typhi can colonize the gallbladder and persist in an asymptomatic carrier state that is frequently associated with the presence of gallstones. We have shown that salmonellae form bile-mediated biofilms on human gallstones and cholesterol-coated surfaces in vitro. Here, we test the hypothesi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1000862107

    authors： Crawford RW,Rosales-Reyes R,Ramírez-Aguilar Mde L,Chapa-Azuela O,Alpuche-Aranda C,Gunn JS

    更新日期：2010-03-02 00:00:00

  • Single d(ApG)/cis-diamminedichloroplatinum(II) adduct-induced mutagenesis in Escherichia coli.

    abstract：:The mutation spectrum induced by the widely used antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP) showed that cisDDP[d(ApG)] adducts, although they account for only 25% of the lesions formed, are approximately 5 times more mutagenic than the major GG adduct. We report the construction of vectors bearing a sin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.16.6087

    authors： Burnouf D,Gauthier C,Chottard JC,Fuchs RP

    更新日期：1990-08-01 00:00:00

  • Impact of daily temperature fluctuations on dengue virus transmission by Aedes aegypti.

    abstract：:Most studies on the ability of insect populations to transmit pathogens consider only constant temperatures and do not account for realistic daily temperature fluctuations that can impact vector-pathogen interactions. Here, we show that diurnal temperature range (DTR) affects two important parameters underlying dengue...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1101377108

    authors： Lambrechts L,Paaijmans KP,Fansiri T,Carrington LB,Kramer LD,Thomas MB,Scott TW

    更新日期：2011-05-03 00:00:00

  • Androgen insensitivity in man: evidence for genetic heterogeneity.

    abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.3.891

    authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

    更新日期：1976-03-01 00:00:00

  • Kinetics of fiber solidification.

    abstract：:Many synthetic or natural fibers are produced via the transformation of a liquid solution into a solid filament, which allows the wet processing of high molecular weight polymers, proteins, or inorganic particles. Synthetic wet-spun fibers are used in our everyday life from clothing to composite reinforcement applicat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1003302107

    authors： Mercader C,Lucas A,Derré A,Zakri C,Moisan S,Maugey M,Poulin P

    更新日期：2010-10-26 00:00:00

  • Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.

    abstract：:Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1808645116

    authors： Akiyama Y,Shinose M,Watanabe H,Yamada S,Kanda Y

    更新日期：2019-04-16 00:00:00

  • Functional differentiation of T cells in the intestine of T cell receptor transgenic mice.

    abstract：:The intestinal lamina propria (LP) is a major effector site of the mucosal immune system where antigen-specific and antigen-nonspecific factors shape the functional responses of CD4+ T helper cells. To study the functional differentiation of LP T helper cells we utilized DO11.10 T cell receptor (TCR) transgenic (Tg) m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.8.3920

    authors： Hurst SD,Sitterding SM,Ji S,Barrett TA

    更新日期：1997-04-15 00:00:00

  • Temperature-sensitive tumorigenicity of cells transformed by a mutant of Moloney sarcoma virus.

    abstract：:Normal rat kidney cells were nonproductively infected either with CP27, a mutant of Moloney sarcoma virus that is temperature-sensitive for maintenance of transformation, or with the parental wild-type virus. The nonproducer cells were inoculated into the tails of athymic nude mice that were subsequently incubated at ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.3.1501

    authors： Klarlund JK,Forchhammer J

    更新日期：1980-03-01 00:00:00

  • Community-based conservation in a globalized world.

    abstract：:Communities have an important role to play in biodiversity conservation. However, community-based conservation as a panacea, like government-based conservation as a panacea, ignores the necessity of managing commons at multiple levels, with vertical and horizontal interplay among institutions. The study of conservatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702098104

    authors： Berkes F

    更新日期：2007-09-25 00:00:00

  • Transformations, transport, and potential unintended consequences of high sulfur inputs to Napa Valley vineyards.

    abstract：:Unintended anthropogenic deposition of sulfur (S) to forest ecosystems has a range of negative consequences, identified through decades of research. There has been far less study of purposeful S use in agricultural systems around the world, including the application of elemental sulfur (S(0)) as a quick-reacting fungi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1110741108

    authors： Hinckley EL,Matson PA

    更新日期：2011-08-23 00:00:00

  • Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice.

    abstract：:Recessive N-ethyl-N-nitrosourea (ENU)-induced mutations recovered at the fitness-1 (fit1) locus in mouse chromosome 7 cause hematopoietic abnormalities, growth retardation, and shortened life span, with varying severity of the defects in different alleles. Abnormal iron distribution and metabolism and frequent scolios...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1432634100

    authors： Klebig ML,Wall MD,Potter MD,Rowe EL,Carpenter DA,Rinchik EM

    更新日期：2003-07-08 00:00:00

  • Minimal mechanistic model of siRNA-dependent target RNA slicing by recombinant human Argonaute 2 protein.

    abstract：:Argonaute (Ago) proteins are the key component of the RNA-induced silencing complex and mediate RNA interference (RNAi) in association with small RNAs. Although overall the mechanism of RNAi is well understood, many molecular details of this complex process are not. Here we report about in-depth steady-state and, in p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1217838110

    authors： Deerberg A,Willkomm S,Restle T

    更新日期：2013-10-29 00:00:00

  • Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells.

    abstract：:Recent evidence indicates there is a role for small membrane vesicles, including exosomes, as vehicles for intercellular communication. Exosomes secreted by most cell types can mediate transfer of proteins, mRNAs, and microRNAs, but their role in the transmission of infectious agents is less established. Recent studie...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1221899110

    authors： Ramakrishnaiah V,Thumann C,Fofana I,Habersetzer F,Pan Q,de Ruiter PE,Willemsen R,Demmers JA,Stalin Raj V,Jenster G,Kwekkeboom J,Tilanus HW,Haagmans BL,Baumert TF,van der Laan LJ

    更新日期：2013-08-06 00:00:00

  • Human settlement of East Polynesia earlier, incremental, and coincident with prolonged South Pacific drought.

    abstract：:The timing of human colonization of East Polynesia, a vast area lying between Hawai'i, Rapa Nui, and New Zealand, is much debated and the underlying causes of this great migration have been enigmatic. Our study generates evidence for human dispersal into eastern Polynesia from islands to the west from around AD 900 an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 历史文章,杂志文章

    doi：10.1073/pnas.1920975117

    authors： Sear DA,Allen MS,Hassall JD,Maloney AE,Langdon PG,Morrison AE,Henderson ACG,Mackay H,Croudace IW,Clarke C,Sachs JP,Macdonald G,Chiverrell RC,Leng MJ,Cisneros-Dozal LM,Fonville T,Pearson E

    更新日期：2020-04-21 00:00:00

  • Fluorescent probe environment and the structural and charge changes in energy coupling of mitochondrial membranes.

    abstract：:The use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. A clear correlation of the probes' fluorescence characteristics with the acquisition of energy coupling...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.67.2.560

    authors： Chance B

    更新日期：1970-10-01 00:00:00

  • Polyglutamine aggregates alter protein folding homeostasis in Caenorhabditis elegans.

    abstract：:Expansion of polyglutamine repeats in several unrelated proteins causes neurodegenerative diseases with distinct but related pathologies. To provide a model system for investigating common pathogenic features, we have examined the behavior of polyglutamine expansions expressed in Caenorhabditis elegans. The expression...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.100107297

    authors： Satyal SH,Schmidt E,Kitagawa K,Sondheimer N,Lindquist S,Kramer JM,Morimoto RI

    更新日期：2000-05-23 00:00:00

  • Nematode sperm maturation triggered by protease involves sperm-secreted serine protease inhibitor (Serpin).

    abstract：:Spermiogenesis is a series of poorly understood morphological, physiological and biochemical processes that occur during the transition of immotile spermatids into motile, fertilization-competent spermatozoa. Here, we identified a Serpin (serine protease inhibitor) family protein (As_SRP-1) that is secreted from sperm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1109912109

    authors： Zhao Y,Sun W,Zhang P,Chi H,Zhang MJ,Song CQ,Ma X,Shang Y,Wang B,Hu Y,Hao Z,Hühmer AF,Meng F,L'hernault SW,He SM,Dong MQ,Miao L

    更新日期：2012-01-31 00:00:00

  • Structure of the body-centered cubic phase of lipid systems does not consist of indefinitely long straight rods.

    abstract：:The observed intensities of the reflections from the body-centered cubic phase of lipid systems are shown to be incompatible with a recently reported model consisting of straight, indefinitely long rods. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.8.4683

    authors： Luzzati V,Tardieu A,Gulik-Krzywicki T

    更新日期：1981-08-01 00:00:00

  • Anomalous anisotropic compression behavior of superconducting CrAs under high pressure.

    abstract：:CrAs was observed to possess the bulk superconductivity under high-pressure conditions. To understand the superconducting mechanism and explore the correlation between the structure and superconductivity, the high-pressure structural evolution of CrAs was investigated using the angle-dispersive X-ray diffraction (XRD)...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1520570112

    authors： Yu Z,Wu W,Hu Q,Zhao J,Li C,Yang K,Cheng J,Luo J,Wang L,Mao HK

    更新日期：2015-12-01 00:00:00

  • Coupling of replication to transcription in vitro.

    abstract：:In a coupled system consisting of RNA polymerase and DNA polymerase I of Escherichia coli, the four deoxyribo- and the four ribonucleoside triphosphates, and DNA of bacteriophage f1 as template, DNA synthesis depends on the concomitant synthesis of RNA. Over a wide range of concentrations of the two polymerases, RNA s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.8.2288

    authors： Karkas JD

    更新日期：1972-08-01 00:00:00

  • Reinitiation involving upstream ORFs regulates ATF4 mRNA translation in mammalian cells.

    abstract：:During cellular stresses, phosphorylation of eukaryotic initiation factor-2 (eIF2) elicits gene expression designed to ameliorate the underlying cellular disturbance. Central to this stress response is the transcriptional regulator activating transcription factor, ATF4. Here we describe the mechanism regulating ATF4 e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400541101

    authors： Vattem KM,Wek RC

    更新日期：2004-08-03 00:00:00

  • Dynamic contrast-enhanced magnetic resonance imaging reveals stress-induced angiogenesis in MCF7 human breast tumors.

    abstract：:The mechanism of contrast enhancement of tumors using magnetic resonance imaging was investigated in MCF7 human breast cancer implanted in nude mice. Dynamic contrast-enhanced images recorded at high spatial resolution were analyzed by an image analysis method based on a physiological model, which included the blood c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.13.6247

    authors： Furman-Haran E,Margalit R,Grobgeld D,Degani H

    更新日期：1996-06-25 00:00:00

  • In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.

    abstract：:We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackiev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.17.6272

    authors： Kandolf R,Ameis D,Kirschner P,Canu A,Hofschneider PH

    更新日期：1987-09-01 00:00:00