Abstract：
:Pancreatic DNase requires both Ca2+ and Mg2+ for its activity as measured by formation of an activated DNA template for in vitro DNA polymerase alpha assay and by the hyperchromic shift. Mn2+ can partially satisfy the Mg2+ requirement of the DNase for activation of DNA but the resulting template is only 50% as active in the DNA polymerase assay. When precautions are taken to avoid divalent ion contamination, pancreatic DNase is not active in the presence of Ca2+ or Mg2+ alone. analysis of the DNA by sucrose gradient centrifugation shows that only in the presence of Ca2+ plus Mg2+ or Mn2+ does pancreatic DNase produce extensive strand breaks in the DNA. The activated DNA template that yields maximal DNA polymerase activity is low molecular weight material of 30,000 to 50,000 daltons.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Baril E,Mitchener J,Lee L,Baril Bdoi
10.1093/nar/4.8.2641subject
Has Abstractpub_date
1977-08-01 00:00:00pages
2641-53issue
8eissn
0305-1048issn
1362-4962journal_volume
4pub_type
杂志文章abstract：:Repression of cellular reprogramming in germ cells is critical to maintaining cell fate and fertility. When germ cells mis-express somatic genes they can be directly converted into other cell types, resulting in loss of totipotency and reproductive potential. Identifying the molecular mechanisms that coordinate these ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa174
更新日期：2020-05-07 00:00:00
abstract：:The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modifi...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkp1117
更新日期：2010-03-01 00:00:00
abstract：:We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Repl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.9.3083
更新日期：1977-09-01 00:00:00
abstract：:We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.5.835
更新日期：1995-03-11 00:00:00
abstract：:Vitamin C breaks DNA only in the presence of oxygen. Superoxide dismutase has no effect on the reaction but catalase suppresses it. Superoxide also gives rise to breaks in DNA suppressible by both superoxide dismutase and catalase. The hydroxyl radical seems to be the agent responsible for strand cleavage itself. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1139
更新日期：1976-05-01 00:00:00
abstract：:Mammalian cryptochromes (Crys) are essential circadian clock factors implicated in diverse clock-independent physiological functions, including DNA damage responses. Here we show that Cry1 modulates the ATR-mediated DNA damage checkpoint (DDC) response by interacting with Timeless (Tim) in a time-of-day-dependent mann...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku094
更新日期：2014-04-01 00:00:00
abstract：:Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.6.1533
更新日期：1976-06-01 00:00:00
abstract：:The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1344
更新日期：2011-05-01 00:00:00
abstract：:The archaeal and eukaryotic evolutionary domains diverged from each other approximately 2 billion years ago, but many of the core components of their transcriptional and translational machineries still display a readily recognizable degree of similarity in their primary structures. The F and P subunits present in arch...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.21.4299
更新日期：2000-11-01 00:00:00
abstract：:The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku690
更新日期：2014-09-01 00:00:00
abstract：:PlantGDB (http://www.plantgdb.org/) is a genomics database encompassing sequence data for green plants (Viridiplantae). PlantGDB provides annotated transcript assemblies for >100 plant species, with transcripts mapped to their cognate genomic context where available, integrated with a variety of sequence analysis tool...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1041
更新日期：2008-01-01 00:00:00
abstract：:The goal of this study was to address antiparallel triplex formation at duplex targets that do not conform to a strict oligopurine.oligopyrimidine motif. We focused on the ability of natural bases and base analogs incorporated into oligonucleotide third strands to bind to so-called CG inversions. These are sites where...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.15.3233
更新日期：1994-08-11 00:00:00
abstract：:All exchangeable protons in a short DNA helix, d(CG)3 sodium salt, have been studied by proton nuclear magnetic resonance. The cytidine and guanosine amino protons have been assigned for the first time. As a function of temperature the cytidine amino protons and the imino protons behave very similarly, their relaxatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.21.8269
更新日期：1984-11-12 00:00:00
abstract：:The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam-Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E. coli ribosomal small subunit rRNA (S-rRNA) has been observ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.23.5779
更新日期：1980-12-11 00:00:00
abstract：:The transactivator responsive region (TAR) present in the 5'-NTR of the HIV-1 genome represents a potential target for antiretroviral intervention and a model system for the development of specific inhibitors of RNA-protein interaction. Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA(TAR)) con...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki743
更新日期：2005-08-02 00:00:00
abstract：:The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.11.4481
更新日期：1987-06-11 00:00:00
abstract：:The synthesis of stable RNA in Mycoplasma capricolum was studied by [32P] labeling of cellular RNA of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. The results indicate that M. capricolum employs the same stringent control mechanism used by E. coli cells, as ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.15.3641
更新日期：1981-08-11 00:00:00
abstract：:DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities. In this work we investigated the in vitro miscoding properties of full-length, human pol lambda either in the absence or in the presence of the human auxiliary proteins proliferating cell n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl032
更新日期：2006-03-06 00:00:00
abstract：:Earlier we have shown that exogenous expression of HIPPI, a molecular partner of Huntingtin interacting protein HIP-1, induces apoptosis and increases expression of caspases-1, -8 and -10 in HeLa and Neuro2A cells. The C-terminal pseudo death effector domain of HIPPI (pDED-HIPPI) specifically interacts with the putati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1011
更新日期：2010-01-01 00:00:00
abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.8.1329
更新日期：1975-08-01 00:00:00
abstract：:Tomato golden mosaic geminivirus has a genome of two single-stranded (ss) DNA components, A and B. An almost identical 'common' region in DNA A and DNA B is thought to contain sequence elements controlling replication and transcription. Hence investigation of sequences important for DNA replication by in vitro mutagen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.24.10213
更新日期：1989-12-25 00:00:00
abstract：:Transcription factor binding sites (TFBSs) are short DNA sequences interacting with transcription factors (TFs), which regulate gene expression. Due to the relatively short length of such binding sites, it is largely unclear how the specificity of protein-DNA interaction is achieved. Here, we have performed a genome-w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl248
更新日期：2006-05-02 00:00:00
abstract：:DNA target recognizing domains of different multispecific DNA-cytosine-methyltransferases can be rearranged through engineering of the corresponding genes to generate enzymes with novel combinations of target recognition. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.14.6649
更新日期：1988-07-25 00:00:00
abstract：:Replication factor C (RF-C) is a eukaryotic heteropentameric protein required for DNA replication and repair processes by loading proliferating cell nuclear antigen (PCNA) onto DNA in an ATP-dependent manner. Prior to loading PCNA, RF-C binds to DNA. This binding is thought to be restricted to a specific DNA structure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.17.3433
更新日期：1999-09-01 00:00:00
abstract：:Protein-induced DNA bending is of importance in the formation of complex nucleoprotein assemblies such as those involved in the initiation of DNA replication or transcription initiation. We have compared the DNA bending characteristics of the Escherichia coli cyclic AMP receptor protein (CRP or CAP), an archetypal DNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.13.3391
更新日期：1992-07-11 00:00:00
abstract：:We have synthesized a family of double-stranded cDNAs (ds cDNAs) using as a template the family of highly purified protamine mRNAs from rainbow trout testis. Individual pure protamine cDNA components were isolated by cloning this family of protamine ds cDNAs in a plasmid vector (pMB9). Clones containing protamine sequ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.6.1463
更新日期：1981-03-25 00:00:00
abstract：:Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1029
更新日期：2020-12-02 00:00:00
abstract：:Clustered damages are formed in DNA by ionising radiation and radiomimetic anticancer agents and are thought to be biologically severe. 7,8-dihydro-8-oxoguanine (8-oxoG), a major DNA damage resulting from oxidative attack, is highly mutagenic leading to a high level of G.C-->T.A transversions if not previously excised...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.5.1107
更新日期：2001-03-01 00:00:00
abstract：:The viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz313
更新日期：2019-07-09 00:00:00
abstract：:In Saccharomyces cerevisiae, the DNA damage response (DDR) is activated by the spatio-temporal colocalization of Mec1-Ddc2 kinase and the 9-1-1 clamp. In the absence of direct means to monitor Mec1 kinase activation in vivo, activation of the checkpoint kinase Rad53 has been taken as a proxy for DDR activation. Here, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1222
更新日期：2010-04-01 00:00:00
