Isolation of glomeruli from areas of bovine cerebellum and comparison of [3H]serotonin uptake.

Abstract:

:A method for bulk preparation of glomerular particles from subdivisions of the bovine cerebellum is presented. This method represents a modification of that previously reported by Hajos et al. [8], which increases the yield of glomerular protein by five-fold (5.5 mg/g wet wt) without compromising structural integrity or homogeneity. In addition, it offers the advantage of allowing one to study intraregional variations in the metabolic properties of cerebellar glomeruli. [3H]Serotonin (5-HT) uptake was measured in this preparation and it was demonstrated that glomeruli possess an active high affinity mechanism for this substrate. Comparison of [3H]5-HT uptake by glomeruli isolated from the cerebellar cortices of the lateral hemispheres and vermis revealed no differences in their kinetic properties.

journal_name

Brain Res Bull

journal_title

Brain research bulletin

authors

Terrian DM,Butcher WI,Wu PH,Armstrong DL

doi

10.1016/0361-9230(85)90025-5

subject

Has Abstract

pub_date

1985-05-01 00:00:00

pages

469-75

issue

5

eissn

0361-9230

issn

1873-2747

pii

0361-9230(85)90025-5

journal_volume

14

pub_type

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