Identification of intracellular degradation intermediates of aldolase B by antiserum to the denatured enzyme.

abstract：:While cells within tissues generate and sense 3D states of strain, the current understanding of the mechanics of fibrous extracellular matrices (ECMs) stems mainly from uniaxial, biaxial, and shear tests. Here, we demonstrate that the multiaxial deformations of fiber networks in 3D cannot be inferred solely based on t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ban E,Wang H,Franklin JM,Liphardt JT,Janmey PA,Shenoy VB

更新日期：2019-04-02 00:00:00

abstract：:Canavan's disease (CD) is a fatal, hereditary disorder of CNS development that has been linked to mutations in the gene for the enzyme aspartoacylase (ASPA) (EC 3.5.1.15). ASPA acts to hydrolyze N-acetylaspartate (NAA) into l-aspartate and acetate, but the connection between ASPA deficiency and the failure of proper C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madhavarao CN,Arun P,Moffett JR,Szucs S,Surendran S,Matalon R,Garbern J,Hristova D,Johnson A,Jiang W,Namboodiri MA

更新日期：2005-04-05 00:00:00

abstract：:Whether G protein-coupled receptors signal from endosomes to control important pathophysiological processes and are therapeutic targets is uncertain. We report that opioids from the inflamed colon activate δ-opioid receptors (DOPr) in endosomes of nociceptors. Biopsy samples of inflamed colonic mucosa from patients an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jimenez-Vargas NN,Gong J,Wisdom MJ,Jensen DD,Latorre R,Hegron A,Teng S,DiCello JJ,Rajasekhar P,Veldhuis NA,Carbone SE,Yu Y,Lopez-Lopez C,Jaramillo-Polanco J,Canals M,Reed DE,Lomax AE,Schmidt BL,Leong KW,Vanner SJ,

更新日期：2020-06-30 00:00:00

abstract：:The D1 reaction center protein of photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chotewutmontri P,Barkan A

更新日期：2020-09-01 00:00:00

abstract：:Mitochondrial protein kinases (mPKs) are molecular switches that down-regulate the oxidation of branched-chain alpha-ketoacids and pyruvate. Elevated levels of these metabolites are implicated in disease states such as insulin-resistant Type II diabetes, branched-chain ketoaciduria, and primary lactic acidosis. We rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Machius M,Chuang JL,Wynn RM,Tomchick DR,Chuang DT

更新日期：2001-09-25 00:00:00

abstract：:Yeast mutants in which genes encoding subunits of the vacuolar H(+)-ATPase were interrupted were assayed for their vacuolar ATPase and proton-uptake activities. The vacuoles from the mutants lacking subunits A (72 kDa), B (57 kDa), or c (proteolipid, 16 kDa) were completely inactive in these reactions. Immunological s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noumi T,Beltrán C,Nelson H,Nelson N

更新日期：1991-03-01 00:00:00

abstract：:The 5'-terminal triphosphate of the 35S RNA isolated from Rous sarcoma virus is blocked by 7-methylguanosine in 5' linkage with the penultimate nucleoside which is methylated in the 2'-O-ribose position, a type of endgroup found in all animal mRNAs investigated during the past year. The specific nuclease-resistant ter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keith J,Fraenkel-Conrat H

更新日期：1975-09-01 00:00:00

abstract：:Despite the growing interest in collective phenomena such as "swarm intelligence" and "wisdom of the crowds," little is known about the mechanisms underlying decision-making in vertebrate animal groups. How do animals use the behavior of others to make more accurate decisions, especially when it is not possible to ide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ward AJ,Sumpter DJ,Couzin ID,Hart PJ,Krause J

更新日期：2008-05-13 00:00:00

abstract：:Entorhinal grid cells integrate sensory and self-motion inputs to provide a spatial metric of a characteristic scale. One function of this metric may be to help localize the firing fields of hippocampal place cells during formation and use of the hippocampal spatial representation ("cognitive map"). Of theoretical imp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casali G,Bush D,Jeffery K

更新日期：2019-03-05 00:00:00

abstract：:Sustained activity of mammalian methionine synthase (MS) requires MS reductase (MSR), but there have been few studies of the interactions between these two proteins. In this study, recombinant human MS (hMS) and MSR (hMSR) were expressed in baculovirus-infected insect cells and purified to homogeneity. hMSR maintained...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada K,Gravel RA,Toraya T,Matthews RG

更新日期：2006-06-20 00:00:00

abstract：:The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stashenko P,Nadler LM,Hardy R,Schlossman SF

更新日期：1981-06-01 00:00:00

abstract：:The mammalian RNase A superfamily comprises a diverse array of ribonucleolytic proteins that have a variety of biochemical activities and physiological functions. Two rapidly evolving RNases of higher primates are of particular interest as they are major secretory proteins of eosinophilic leukocytes and have been foun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang J,Dyer KD,Rosenberg HF

更新日期：2000-04-25 00:00:00

abstract：:Selective blockade of gene expression by designed small molecules is a fundamental challenge at the interface of chemistry, biology, and medicine. Transcription factors have been among the most elusive targets in genetics and drug discovery, but the fields of chemical biology and genetics have evolved to a point where...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kushal S,Lao BB,Henchey LK,Dubey R,Mesallati H,Traaseth NJ,Olenyuk BZ,Arora PS

更新日期：2013-09-24 00:00:00

abstract：:It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberley TD,Lauchner LJ,Pugh TD,Gonzalez A,Goldfarb S,Li SA,Li JJ

更新日期：1989-03-01 00:00:00

abstract：:Three proteins from cyanobacteria (KaiA, KaiB, and KaiC) can reconstitute circadian oscillations in vitro. At least three molecular properties oscillate during this reaction, namely rhythmic phosphorylation of KaiC, ATP hydrolytic activity of KaiC, and assembly/disassembly of intermolecular complexes among KaiA, KaiB,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin X,Byrne M,Mori T,Zou P,Williams DR,McHaourab H,Johnson CH

更新日期：2010-08-17 00:00:00

abstract：:The T helper type 2 (Th2) cytokine genes Il4, Il5, and Il13 are contained within a 140-kb region of mouse chromosome 11 and their expression is controlled by a locus control region (LCR) embedded within this locus. The LCR is composed of a number of DNase I-hypersensitive sites (HSs), which are believed to encompass t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams A,Lee GR,Spilianakis CG,Hwang SS,Eisenbarth SC,Flavell RA

更新日期：2013-04-23 00:00:00

abstract：:Proinflammatory signaling pathways are commonly up-regulated in breast cancer. In estrogen receptor-negative (ER-) and triple-negative breast cancer (TNBC), nitric oxide synthase-2 (NOS2) and cyclooxygenase-2 (COX2) have been described as independent predictors of disease outcome. We further explore these findings by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Basudhar D,Glynn SA,Greer M,Somasundaram V,No JH,Scheiblin DA,Garrido P,Heinz WF,Ryan AE,Weiss JM,Cheng RYS,Ridnour LA,Lockett SJ,McVicar DW,Ambs S,Wink DA

更新日期：2017-12-05 00:00:00

abstract：:Stressor exposure biases decision-making strategies from those based on the relationship between actions and their consequences to others restricted by stimulus-response associations. Chronic stressor exposure also desensitizes glucocorticoid receptors (GR) and diminishes motivation to acquire food reinforcement, alth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gourley SL,Swanson AM,Jacobs AM,Howell JL,Mo M,Dileone RJ,Koleske AJ,Taylor JR

更新日期：2012-12-11 00:00:00

abstract：:This paper reports the results of a randomized field experiment involving registered voters in the city of New Haven. Nonpartisan get-out-the-vote messages were delivered through personal canvassing shortly before the November 1998 election. We find that personal canvassing increased voter turnout by approximately 6. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerber AS,Green DP

更新日期：1999-09-14 00:00:00

abstract：:Recent research has emphasized the importance of the metabolic cluster, which includes glucose intolerance, dyslipidemia, and high blood pressure, as a strong predictor of the obesity-related morbidities and premature mortality. Fundamental to this association, commonly referred to as the metabolic syndrome, is the cl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kissebah AH,Sonnenberg GE,Myklebust J,Goldstein M,Broman K,James RG,Marks JA,Krakower GR,Jacob HJ,Weber J,Martin L,Blangero J,Comuzzie AG

更新日期：2000-12-19 00:00:00

abstract：:UDP-xylose is a sugar donor required for the synthesis of diverse and important glycan structures in animals, plants, fungi, and bacteria. Xylose-containing glycans are particularly abundant in plants and in the polysaccharide capsule that is the major virulence factor of the pathogenic fungus Cryptococcus neoformans....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bar-Peled M,Griffith CL,Doering TL

更新日期：2001-10-09 00:00:00

abstract：:Proteins, nucleic acids, and fluorescein-conjugated antibody are shown to be identifidable in situ via the fluorescence excited by the focused electron beam of a canning electron microscope. A molecular species is identified by its characteristic fluorescence spectrum and by a characteristic alteration of the spectrum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hough PV,McKinney WR,Ledbeter MC,Pollack RE,Moos HW

更新日期：1976-02-01 00:00:00

abstract：:The exemplary search capabilities of flying insects have established them as one of the most diverse taxa on Earth. However, we still lack the fundamental ability to quantify, represent, and predict trajectories under natural contexts to understand search and its applications. For example, flying insects have evolved ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaushik PK,Renz M,Olsson SB

更新日期：2020-06-02 00:00:00

abstract：:In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doelling JH,Gaudino RJ,Pikaard CS

更新日期：1993-08-15 00:00:00

abstract：:cDNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by screening with a pool of sera from patients with invasive amoebiasis that had been absorbed with nonpathogenic amoebae. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tannich E,Horstmann RD,Knobloch J,Arnold HH

更新日期：1989-07-01 00:00:00

abstract：:A ribosomal RNA-encoding DNA (rDNA) cloned sequence, consisting of a 0.8-kilobase fragment from the 26S/nontranscribed spacer region, was used to identify diagnostic restriction enzyme fragments that distinguish the Moreton and Torresian subspecies of the grasshopper Caledia captiva. These restriction fragments were t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnold ML,Shaw DD,Contreras N

更新日期：1987-06-01 00:00:00

abstract：:The three-dimensional structures of RNA enzymes form catalytic centers that include specific substrate binding sites. High-resolution determination of these and other RNA structures is essential for a detailed understanding of the function of RNA in biological systems. The crystal structures of only a few RNA molecule...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doudna JA,Grosshans C,Gooding A,Kundrot CE

更新日期：1993-08-15 00:00:00

abstract：:Whereas all mammals have one glutamate dehydrogenase gene (GLUD1), humans and apes carry an additional gene (GLUD2), which encodes an enzyme with distinct biochemical properties. We inserted a bacterial artificial chromosome containing the human GLUD2 gene into mice and analyzed the resulting changes in the transcript...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Q,Guo S,Jiang X,Bryk J,Naumann R,Enard W,Tomita M,Sugimoto M,Khaitovich P,Pääbo S

更新日期：2016-05-10 00:00:00

abstract：:In recent years, a large body of research has demonstrated that judgments and behaviors can propagate from person to person. Phenomena as diverse as political mobilization, health practices, altruism, and emotional states exhibit similar dynamics of social contagion. The precise mechanisms of judgment propagation are ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moussaïd M,Herzog SM,Kämmer JE,Hertwig R

更新日期：2017-04-18 00:00:00

abstract：:A molecular genetic approach employing polymorphic DNA markers has been used to investigate the role of chromosomal aberrations in meningioma, one of the most common tumors of the human nervous system. Comparison of the alleles detected by DNA markers in tumor DNA versus DNA from normal tissue revealed chromosomal alt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seizinger BR,de la Monte S,Atkins L,Gusella JF,Martuza RL

更新日期：1987-08-01 00:00:00