Mechanism of ribosomal translocation. Translocation limits the rate of Escherichia coli elongation factor G-promoted GTP hydrolysis.

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:Gene transcription is regulated in response to environmental changes and developmental cues. In mammalian cells subjected to stress conditions such as heat shock, transcription of most protein-coding genes decreases, while the transcription of heat shock protein genes increases. Repression involves direct binding to R...

journal_title：Journal of molecular biology

authors： Kassube SA,Fang J,Grob P,Yakovchuk P,Goodrich JA,Nogales E

更新日期：2013-10-09 00:00:00

abstract：:Influenza viral entry into the host cell cytoplasm is accomplished by a process of membrane fusion mediated by the viral hemagglutinin protein. Hemagglutinin acts in a pH-triggered fashion, inserting a short fusion peptide into the host membrane followed by refolding of a coiled-coil structure to draw the viral envelo...

journal_title：Journal of molecular biology

authors： Zawada KE,Okamoto K,Kasson PM

更新日期：2018-03-02 00:00:00

abstract：:One mechanism for the repair of UV-induced DNA damage is the base excision repair pathway. The initial step in this pathway and the specificity for the type of damage that is to be repaired reside in DNA glycosylase/abasic (AP) lyases. Cleavage of the glycosyl bond of the 5' pyrimidine of a cyclobutane pyrimidine dime...

journal_title：Journal of molecular biology

authors： Garvish JF,Lloyd RS

更新日期：2000-01-21 00:00:00

abstract：:Oncogenes may play a crucial role in the genetic program of cellular differentiation; even, probably, at a very early stage in this program, which can be described as pre-commitment. We have investigated the variation in, and the control level of, the accumulation of the transformation-associated cellular protein p53 ...

journal_title：Journal of molecular biology

authors： Khochbin S,Principaud E,Chabanas A,Lawrence JJ

更新日期：1988-03-05 00:00:00

abstract：:The lateral diffusion coefficient of fluorescent lipid analogues incorporated in four cubic phases of lipid-water systems was determined by the modulated fringe pattern photobleaching technique. In two of the phases, Q230 and Q224, whose structure is bicontinuous, the diffusion is almost as fast as in the fluid lipid ...

journal_title：Journal of molecular biology

authors： Cribier S,Gulik A,Fellmann P,Vargas R,Devaux PF,Luzzati V

更新日期：1993-01-20 00:00:00

abstract：:A Rhizobium meliloti DNA region (nodD1) involved in the regulation of other early nodulation genes has been delimited by directed Tn5 mutagenesis and its nucleotide sequence has been determined. The sequence data indicate a large open reading frame with opposite polarity to nodA, -B and -C, coding for a protein of 308...

journal_title：Journal of molecular biology

authors： Göttfert M,Horvath B,Kondorosi E,Putnoky P,Rodriguez-Quiñones F,Kondorosi A

更新日期：1986-10-05 00:00:00

abstract：:Annexins constitute a family of phospholipid- and Ca(2+)-binding proteins involved in a variety of membrane-related processes. The property of several annexins, including annexin A5, to self-organize at the surface of lipid membranes into 2D ordered arrays has been proposed to be functionally relevant in cellular cont...

journal_title：Journal of molecular biology

authors： Oling F,Santos JS,Govorukhina N,Mazères-Dubut C,Bergsma-Schutter W,Oostergetel G,Keegstra W,Lambert O,Lewit-Bentley A,Brisson A

更新日期：2000-12-08 00:00:00

abstract：:The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an excised Ssp DnaE intein and the second from a splicing-deficient precu...

journal_title：Journal of molecular biology

authors： Sun P,Ye S,Ferrandon S,Evans TC,Xu MQ,Rao Z

更新日期：2005-11-11 00:00:00

abstract：:Proteolysis of rat liver chromatin by the Arg-C peptidase, clostripain, is characterized by a progressive fragmentation of the N-terminal segments of the four core histones H2A, H2B, H3 and H4, until a well-defined limit digest is reached. This work addresses the case of histone H4. Two intermediate proteolytic sites ...

journal_title：Journal of molecular biology

authors： Banères JL,Essalouh L,Jariel-Encontre I,Mesnier D,Garrod S,Parello J

更新日期：1994-10-14 00:00:00

abstract：:Acireductone dioxygenase (ARD) catalyzes different reactions between O2 and 1,2-dihydroxy-3-oxo-5-(methylthio)pent-1-ene (acireductone) depending upon the metal bound in the active site. Ni2+ -ARD cleaves acireductone to formate, CO and methylthiopropionate. If Fe2+ is bound (ARD'), the same substrates yield methylthi...

journal_title：Journal of molecular biology

authors： Ju T,Goldsmith RB,Chai SC,Maroney MJ,Pochapsky SS,Pochapsky TC

更新日期：2006-11-03 00:00:00

abstract：:DNA polymerases that initiate replication by protein-priming are able to catalyze terminal protein (TP)-primed initiation, the following transition steps and finally DNA-primed elongation. Therefore, their structures must be able to position sequentially both primers, TP and DNA, at a common binding site. For DNA-temp...

journal_title：Journal of molecular biology

authors： Truniger V,Lázaro JM,Blanco L,Salas M

更新日期：2002-04-19 00:00:00

abstract：:Human cytidine deaminase apolipoprotein B mRNA-editing catalytic polypeptide-like 3F (APOBEC3F, or A3F), like APOBEC3G, has broad antiviral activity against diverse retroelements, including Vif-deficient human immunodeficiency virus (HIV)-1. Its antiviral functions are known to rely on its virion encapsidation and be ...

journal_title：Journal of molecular biology

authors： Wang T,Tian C,Zhang W,Sarkis PT,Yu XF

更新日期：2008-01-25 00:00:00

abstract：:The amide proton exchange rates of Escherichia coli trp repressor have been measured through their effects on the longitudinal relaxation rates of the amide protons. Three types of exchange regimes have been observed: (1) slow exchange (on a minute/hour time-scale), measurable by isotope exchange, but not by relaxatio...

journal_title：Journal of molecular biology

authors： Gryk MR,Finucane MD,Zheng Z,Jardetzky O

更新日期：1995-03-10 00:00:00

abstract：:Bovine leukemia virus (BLV) is a deltaretrovirus that infects domestic cattle. The structural protein Gag, found in all retroviruses, is a polyprotein comprising three major functional domains: matrix (MA), capsid (CA), and nucleocapsid (NC). Previous studies have shown that both mature BLV MA and NC are able to bind ...

journal_title：Journal of molecular biology

authors： Qualley DF,Cooper SE,Ross JL,Olson ED,Cantara WA,Musier-Forsyth K

更新日期：2019-03-15 00:00:00

abstract：:Computational searches for DNA binding sites often utilize consensus sequences. These search models make assumptions that the frequency of a base pair in an alignment relates to the base pair's importance in binding and presume that base pairs contribute independently to the overall interaction with the DNA-binding pr...

journal_title：Journal of molecular biology

authors： Wozniak CE,Hughes KT

更新日期：2008-06-20 00:00:00

abstract：:The expression of antibodies inside cells to ablate protein function has the potential for disease therapy and for target validation in functional genomics. However, due to inefficient expression or folding, only a few antibodies or antibody fragments, usually as single-chain Fv antibody fragments (scFv), bind their a...

journal_title：Journal of molecular biology

authors： Tse E,Lobato MN,Forster A,Tanaka T,Chung GT,Rabbitts TH

更新日期：2002-03-15 00:00:00

abstract：:We have developed a computer model for the simulation of simultaneous SELEX against multiple targets. The model assumes equilibrium behavior for the formation of binary ligand:target complexes, and that there is no ligand:ligand or target:target interaction. Target concentrations, ligand concentrations, and affinity d...

journal_title：Journal of molecular biology

authors： Vant-Hull B,Payano-Baez A,Davis RH,Gold L

更新日期：1998-05-08 00:00:00

abstract：:X-ray data have been recorded to 1.0 A resolution from a crystal of Fusarium solani cutinase using synchrotron radiation and an imaging-plate scanner. The anisotropic treatment of thermal motion led to a fivefold increase in accuracy and to a considerable quality improvement in the electron density maps with respect t...

journal_title：Journal of molecular biology

authors： Longhi S,Czjzek M,Lamzin V,Nicolas A,Cambillau C

更新日期：1997-05-16 00:00:00

abstract：:The Spo0A protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of Spo0A is absolutely necessary for the initiation of sporulation. We now show that phospho-Spo0A is a dimer, binds specifically ...

journal_title：Journal of molecular biology

authors： Asayama M,Yamamoto A,Kobayashi Y

更新日期：1995-06-30 00:00:00

abstract：:At low ionic strength, the SfiI restriction enzyme cleaved at similar rates both supercoiled and linear DNA with two SfiI sites and linear DNA with one SfiI site. For the substrates with two sites, the majority of the DNA was converted directly to products cut at both sites; the enzyme appears to bind to two sites bef...

journal_title：Journal of molecular biology

authors： Nobbs TJ,Halford SE

更新日期：1995-09-29 00:00:00

abstract：:High amphiphilicity is a hallmark of interfacial helices in membrane proteins and membrane-active peptides, such as toxins and antimicrobial peptides. Although there is general agreement that amphiphilicity is important for membrane-interface binding, an unanswered question is its importance relative to simple hydroph...

journal_title：Journal of molecular biology

authors： Fernández-Vidal M,Jayasinghe S,Ladokhin AS,White SH

更新日期：2007-07-13 00:00:00

abstract：:Chromatin compacts genomic DNA in eukaryotes. The primary chromatin unit is the nucleosome core particle, composed of four pairs of the core histones, H2A, H2B, H3, and H4, and 145-147 base pairs of DNA. Since replication, recombination, repair, and transcription take place in chromatin, the structure and dynamics of ...

journal_title：Journal of molecular biology

authors： Kurumizaka H,Kujirai T,Takizawa Y

更新日期：2020-10-14 00:00:00

abstract：:Catalytic and other functionally important residues in proteins can often be mutated to yield more stable proteins. Many of these residues are charged residues that are located in electrostatically unfavorable environments. Here it is demonstrated that because continuum electrostatics methods can identify these destab...

journal_title：Journal of molecular biology

authors： Elcock AH

更新日期：2001-09-28 00:00:00

abstract：:We had previously used in vitro RNA selection techniques to describe a consensus RNA pseudoknot that binds and inhibits HIV-1 reverse transcriptase (HIV-RT). In this work we constructed variants of this consensus pseudoknot in order to evaluate the contributions of individual nucleotide identities and secondary struct...

journal_title：Journal of molecular biology

authors： Green L,Waugh S,Binkley JP,Hostomska Z,Hostomsky Z,Tuerk C

更新日期：1995-03-17 00:00:00

abstract：:Long-standing questions on how peptides fold are addressed by the simulation at different temperatures of the reversible folding of a peptide in solution in atomic detail. Molecular dynamics simulations correctly predict the structure that is thermodynamically stable at 298 K, irrespective of the initial peptide confo...

journal_title：Journal of molecular biology

authors： Daura X,Jaun B,Seebach D,van Gunsteren WF,Mark AE

更新日期：1998-07-31 00:00:00

abstract：:Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see tex...

journal_title：Journal of molecular biology

authors： Miller M,Wlodawer A,Appella E,Sussman JL

更新日期：1987-06-20 00:00:00

abstract：:Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inacti...

journal_title：Journal of molecular biology

authors： Gordon-Smith DJ,Carbajo RJ,Yang JC,Videler H,Runswick MJ,Walker JE,Neuhaus D

更新日期：2001-04-27 00:00:00

abstract：:The crystal structure of the staphylococcal nuclease mutant V66K, in which valine 66 is replaced by lysine, has been solved at 1.97 A resolution. Unlike lysine residues in previously reported protein structures, this residue appears to bury its side-chain in the hydrophobic core without salt bridging, hydrogen bonding...

journal_title：Journal of molecular biology

authors： Stites WE,Gittis AG,Lattman EE,Shortle D

更新日期：1991-09-05 00:00:00

abstract：:Numerous mitochondrial quality control mechanisms exist within cells, but none have been shown to effectively assess and control the quality of mitochondrial DNA (mtDNA). One reason such mechanisms have yet to be elucidated is that they do not appear to be particularly active in most somatic cells, where many studies ...

journal_title：Journal of molecular biology

authors： Palozzi JM,Jeedigunta SP,Hurd TR

更新日期：2018-12-07 00:00:00