DNase I-hypersensitive sites surround the mouse acetylcholine receptor delta-subunit gene.

abstract：:The development of cardiac hypertrophy is mediated, in part, by increase in NADPH oxidase activity and myocardial oxidative stress. The Rho GTPase, Rac, regulates NADPH oxidase activity through interaction with gp91(phox) and p67(phox) (in which "phox" is phagocyte oxidase). However, it is not known which Rac isoform ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satoh M,Ogita H,Takeshita K,Mukai Y,Kwiatkowski DJ,Liao JK

更新日期：2006-05-09 00:00:00

abstract：:The recent demonstration of K+ channel dysfunction in fibroblasts from Alzheimer disease (AD) patients and past observations of Ca(2+)-mediated K+ channel modulation during memory storage suggested that AD, which is characterized by memory loss and other cognitive deficits, might also involve dysfunction of intracellu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ito E,Oka K,Etcheberrigaray R,Nelson TJ,McPhie DL,Tofel-Grehl B,Gibson GE,Alkon DL

更新日期：1994-01-18 00:00:00

abstract：:CYP1B1-null mice, created by targeted gene disruption in embryonic stem cells, were born at the expected frequency from heterozygous matings with no observable phenotype, thus establishing that CYP1B1 is not required for mouse development. CYP1B1 was not detectable in cultured embryonic fibroblast (EF) or in different...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buters JT,Sakai S,Richter T,Pineau T,Alexander DL,Savas U,Doehmer J,Ward JM,Jefcoate CR,Gonzalez FJ

更新日期：1999-03-02 00:00:00

abstract：:Wild-type newborn mice are characterized by the ability of certain liver-specific genes encoding various enzymes and mapping on different chromosomes to respond to glucocorticoid induction. Newborn mice homozygous for deletions at and around the albino locus on chromosome 7 fail to develop this competence for hormone-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeFranco D,Bali D,Torres R,DePinho RA,Erickson RP,Gluecksohn-Waelsch S

更新日期：1991-07-01 00:00:00

abstract：:To assess the contribution to immunoglobulin heavy chain diversity made by recombination between variable region (VH) genes and joining region (JH) genes, we have determined the sequence of about 2000 nucleotides spanning the rearranged JH gene cluster associated with the VH gene expressed in plasmacytoma HPC76. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gough NM,Bernard O

更新日期：1981-01-01 00:00:00

abstract：:The type specimen for Australopithecus africanus (Taung) includes a natural endocast that reproduces most of the external morphology of the right cerebral hemisphere and a fragment of fossilized face that articulates with the endocast. Despite the fact that Taung died between 3 and 4 y of age, the endocast reproduces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Falk D,Zollikofer CP,Morimoto N,Ponce de León MS

更新日期：2012-05-29 00:00:00

abstract：:I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the matur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutcliffe JG

更新日期：1978-08-01 00:00:00

abstract：:The accuracy of the 3'-end processing by cleavage and polyadenylation is essential for mRNA biogenesis and transcription termination. In yeast, two poorly conserved neighboring elements upstream of cleavage sites are important for accuracy and efficiency of this process. These two RNA sequences are recognized by the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnwal RP,Lee SD,Moore C,Varani G

更新日期：2012-12-26 00:00:00

abstract：:Phosphorylation of ADP to ATP was induced in nonrespiring submitochondrial particles (SMP) from rat liver by the application of electric pulses with field strengths of 10-35 kV/cm and a decay time of 60 mus. In all cases respiration was inhibited completely by using cyanide or rotenone. Newly formed ATP was measured b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teissie J,Knox BE,Tsong TY,Wehrle J

更新日期：1981-12-01 00:00:00

abstract：:Highly pathogenic avian influenza A viruses of subtypes H5 and H7 are the causative agents of fowl plague in poultry. Influenza A viruses of subtype H5N1 also caused severe respiratory disease in humans in Hong Kong in 1997 and 2003, including at least seven fatal cases, posing a serious human pandemic threat. Between...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fouchier RA,Schneeberger PM,Rozendaal FW,Broekman JM,Kemink SA,Munster V,Kuiken T,Rimmelzwaan GF,Schutten M,Van Doornum GJ,Koch G,Bosman A,Koopmans M,Osterhaus AD

更新日期：2004-02-03 00:00:00

abstract：:Clostridium difficile has rapidly emerged as the leading cause of antibiotic-associated diarrheal disease, with the transcontinental spread of various PCR ribotypes, including 001, 017, 027 and 078. However, the genetic basis for the emergence of C. difficile as a human pathogen is unclear. Whole genome sequencing was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He M,Sebaihia M,Lawley TD,Stabler RA,Dawson LF,Martin MJ,Holt KE,Seth-Smith HM,Quail MA,Rance R,Brooks K,Churcher C,Harris D,Bentley SD,Burrows C,Clark L,Corton C,Murray V,Rose G,Thurston S,van Tonder A,Walker D

更新日期：2010-04-20 00:00:00

abstract：:We introduced radioactive precursors directly into identified neurons of Aplysia californica. [(3)H]-Choline and L-[(3)H]tryptophan were injected with pressure into nerve cell bodies to study synthesis of acetylcholine and serotonin. We confirmed the cholinergic nature of R2, L10, and L11, identified neurons of the ab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenstadt M,Goldman JE,Kandel ER,Koike H,Koester J,Schwartz JH

更新日期：1973-12-01 00:00:00

abstract：:Reactive oxygen species (ROS) are well-known accelerants of aging, but, paradoxically, we show that physiological levels of ROS extend life span in pupae of the moth Helicoverpa armigera, resulting in the dormant state of diapause. This developmental switch appears to operate through a variant of the conventional insu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang XS,Wang T,Lin XW,Denlinger DL,Xu WH

更新日期：2017-09-12 00:00:00

abstract：:Double-stranded RNA (dsRNA) recently has been shown to give rise to genetic interference in Caenorhabditis elegans and also is likely to be the basis for phenotypic cosuppression in plants in certain instances. While constructing a plasmid vector for transfection of trypanosome cells, we serendipitously discovered tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ngô H,Tschudi C,Gull K,Ullu E

更新日期：1998-12-08 00:00:00

abstract：:Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of exce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niyogi KK,Björkman O,Grossman AR

更新日期：1997-12-09 00:00:00

abstract：:Studies on the structure-function relationship of diphtheria toxin are reported. New methods are described for the preparation of pure intact ("unnicked") toxin and for the preparation of the individual A and B chains. A biological assay method for the B chain is also presented, as well as a method for the labelling o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Everse J,Lappi DA,Beglau JM,Lee CL,Kaplan NO

更新日期：1977-02-01 00:00:00

abstract：:The C-terminal 179-aa region of yeast (Saccharomyces cerevisiae) TATA-binding protein (TBP), phylogenetically conserved and sufficient for many functions, formed crystals diffracting to 1.7-A resolution. The structure of the protein, determined by molecular replacement with coordinates from Arabidopsis TBP and refined...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chasman DI,Flaherty KM,Sharp PA,Kornberg RD

更新日期：1993-09-01 00:00:00

abstract：:The ability to intercept uncooperative targets is key to many diverse flight behaviors, from courtship to predation. Previous research has looked for simple geometric rules describing the attack trajectories of animals, but the underlying feedback laws have remained obscure. Here, we use GPS loggers and onboard video ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brighton CH,Thomas ALR,Taylor GK

更新日期：2017-12-19 00:00:00

abstract：:The rat brain-derived neurotropic factor (BDNF) gene consists of four 5' exons linked to separate promoters and one 3' exon encoding the prepro-BDNF protein. To gain insights into the regulation of BDNF mRNA expression, probes specific for the different 5' exons were used to study the expression of BDNF mRNA in the br...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Metsis M,Timmusk T,Arenas E,Persson H

更新日期：1993-10-01 00:00:00

abstract：:pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F and ColE1. Fragment B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Figurski DH,Helinski DR

更新日期：1979-04-01 00:00:00

abstract：:Defined by monoclonal antibody GA733, the GA733-2 antigen is a cell surface 40-kDa glycoprotein associated with human carcinomas of various origins. Molecular clones for the GA733-2 antigen were isolated from a colorectal carcinoma cell line cDNA library using the high-efficiency COS cell expression system. A 1.4-kilo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szala S,Froehlich M,Scollon M,Kasai Y,Steplewski Z,Koprowski H,Linnenbach AJ

更新日期：1990-05-01 00:00:00

abstract：:We have carried out free energy perturbation calculations on DNA double-stranded hexanucleotides. The sequence d(CGCGCG)2 has been "mutated" into d(CGTGCG).d(CGCACG) with the oligonucleotide in the A, B, and Z structural forms, both in vacuo and in aqueous solution. In addition, model free energy calculations have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dang LX,Pearlman DA,Kollman PA

更新日期：1990-06-01 00:00:00

abstract：:One can imagine a variety of mechanisms that should result in self-perpetuating biological states. It is generally assumed that cytosine methylation is propagated in eukaryotes by enzymes that specifically methylate hemimethylated symmetrical sites (e.g., (5')CpGGpC(5') or (5')CpNpGGpNpC(5')). Although there is wide s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Selker EU,Freitag M,Kothe GO,Margolin BS,Rountree MR,Allis CD,Tamaru H

更新日期：2002-12-10 00:00:00

abstract：:Insertion mutations previously constructed within the proximal region of the yeast invertase signal sequence did not interfere with secretion or glycosylation of the enzyme. We now describe deletion mutations within the same signal sequence. Large deletions truncating the hydrophobic core of the signal peptide prevent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perlman D,Raney P,Halvorson HO

更新日期：1986-07-01 00:00:00

abstract：:Two-dimensional molecular aggregate (2DMA), a thin sheet of strongly interacting dipole molecules self-assembled at close distance on an ordered lattice, is a fascinating fluorescent material. It is distinctively different from the conventional (single or colloidal) dye molecules and quantum dots. In this paper, we ve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Q,Jin D,Xiao J,Nam SH,Liu X,Liu Y,Zhang X,Fang NX

更新日期：2017-09-19 00:00:00

abstract：:Using data from the North American Breeding Bird Survey, we determined that most neotropical migrant bird species that breed in forests of the eastern United States and Canada have recently (1978-1987) declined in abundance after a period of stable or increasing populations. Most permanent residents and temperate-zone...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robbins CS,Sauer JR,Greenberg RS,Droege S

更新日期：1989-10-01 00:00:00

abstract：:A previous study demonstrated that the translational inhibitor from lysates of heme-deficient rabbit reticulocytes is associated with a protein kinase activity. Chromatography of this inhibitor preparation on phosphocellulose yields two distinct protein kinase activities, PC1 and PC2. PC1, which consitutes about 90% o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levin D,Ranu RS,Ernst V,London IM

更新日期：1976-09-01 00:00:00

abstract：:Disruption of the blood-brain barrier (BBB) underlies the development of experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis. Environmental factors, such as Bordetella pertussis, are thought to sensitize central endothelium to biogenic amines like histamine, thereby leading to increased BBB permeabi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu C,Diehl SA,Noubade R,Ledoux J,Nelson MT,Spach K,Zachary JF,Blankenhorn EP,Teuscher C

更新日期：2010-11-02 00:00:00

abstract：:The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of repli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami Y,Wobbe CR,Weissbach L,Dean FB,Hurwitz J

更新日期：1986-05-01 00:00:00

abstract：:Developmental and physiological responses are regulated by light throughout the entire life cycle of higher plants. To sense changes in the light environment, plants have developed various photoreceptors, including the red/far-red light-absorbing phytochromes and blue light-absorbing cryptochromes. A wide variety of p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bognár LK,Hall A,Adám E,Thain SC,Nagy F,Millar AJ

更新日期：1999-12-07 00:00:00