Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2.

Abstract:

:Defined by monoclonal antibody GA733, the GA733-2 antigen is a cell surface 40-kDa glycoprotein associated with human carcinomas of various origins. Molecular clones for the GA733-2 antigen were isolated from a colorectal carcinoma cell line cDNA library using the high-efficiency COS cell expression system. A 1.4-kilobase cDNA species was enriched by immunoselection with monoclonal antibody. The authenticity of individual clones was established by immunologic and sequence criteria. At the amino acid sequence level, GA733-2 was found to be greater than 99% identical to the previously described KSA antigen defined by monoclonal antibody KS1/4. The amino acid sequence derived from the previously described GA733-related gene, GA733-1, was found to be 49% identical to GA733-2. The positions of 12 cysteine residues in the extracellular domains of the two GA733 antigens are conserved, as is the overall distribution of hydrophobic and hydrophilic residues. A 1.45-kilobase transcript of the GA733-2/KSA gene was found to be expressed in cell lines derived from colorectal and pancreatic carcinoma.

authors

Szala S,Froehlich M,Scollon M,Kasai Y,Steplewski Z,Koprowski H,Linnenbach AJ

doi

10.1073/pnas.87.9.3542

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

3542-6

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

87

pub_type

杂志文章
  • Amphiregulin: a bifunctional growth-modulating glycoprotein produced by the phorbol 12-myristate 13-acetate-treated human breast adenocarcinoma cell line MCF-7.

    abstract::A glycoprotein, termed amphiregulin (AR), inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. It has been purified to apparent homogeneity from serum-free conditioned medium of MCF-7 human breast carcinoma cells that had been trea...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.17.6528

    authors: Shoyab M,McDonald VL,Bradley JG,Todaro GJ

    更新日期:1988-09-01 00:00:00

  • Platelet-derived growth factor gene expression in human atherosclerotic plaques and normal artery wall.

    abstract::We previously demonstrated that the B chain of platelet-derived growth factor (PDGF-B) is transcribed in human atherosclerotic plaques, indicating that production of growth factors within plaques could occur during atherogenesis. However, since atherosclerotic plaques are composed of several cell types and three of th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.8.2810

    authors: Barrett TB,Benditt EP

    更新日期:1988-04-01 00:00:00

  • Franck-Condon picture of incoherent neutron scattering.

    abstract::A spectroscopic interpretation of incoherent neutron scattering experiments is presented which is based on Franck-Condon-type probabilities for scattering-induced transitions between quantum states of the target. The resulting expressions for the scattering functions enable an energy landscape-oriented analysis of neu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1718720115

    authors: Kneller GR

    更新日期:2018-09-18 00:00:00

  • Four hundred-million-year-old vesicular arbuscular mycorrhizae.

    abstract::The discovery of arbuscules in Aglaophyton major, an Early Devonian land plant, provides unequivocal evidence that mycorrhizae were established >400 million years ago. Nonseptate hyphac and arbuscules occur in a specialized meristematic region of the cortex that continually provided new cells for fungal infection. Arb...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.25.11841

    authors: Remy W,Taylor TN,Hass H,Kerp H

    更新日期:1994-12-06 00:00:00

  • Glucose-dependent insulinotropic peptide: structure of the precursor and tissue-specific expression in rat.

    abstract::Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid gastrointestinal regulatory peptide that stimulates insulin secretion from pancreatic beta cells in the presence of glucose. Approximately 7.8 x 10(5) recombinant clones of a neonatal rat intestinal cDNA library were screened by using plaque hybridizati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.5.1992

    authors: Tseng CC,Jarboe LA,Landau SB,Williams EK,Wolfe MM

    更新日期:1993-03-01 00:00:00

  • Structure of the rare archaeal biosphere and seasonal dynamics of active ecotypes in surface coastal waters.

    abstract::Marine Archaea are important players among microbial plankton and significantly contribute to biogeochemical cycles, but details regarding their community structure and long-term seasonal activity and dynamics remain largely unexplored. In this study, we monitored the interannual archaeal community composition of abun...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1216863110

    authors: Hugoni M,Taib N,Debroas D,Domaizon I,Jouan Dufournel I,Bronner G,Salter I,Agogué H,Mary I,Galand PE

    更新日期:2013-04-09 00:00:00

  • Nonsensory target-dependent organization of piriform cortex.

    abstract::The piriform cortex (PCX) is the largest component of the olfactory cortex and is hypothesized to be the locus of odor object formation. The distributed odorant representation found in PCX contrasts sharply with the topographical representation seen in other primary sensory cortices, making it difficult to test this v...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1411266111

    authors: Chen CF,Zou DJ,Altomare CG,Xu L,Greer CA,Firestein SJ

    更新日期:2014-11-25 00:00:00

  • Wide distribution of immunoreactive renin in nerve cells of human brain.

    abstract::By use of the indirect peroxidase-antiperoxidase complex immunocytochemical technique, antibody to purified human renal renin was applied to formalin-fixed paraffin sections of human cadaver brain. Immune reaction products were observed in most nerve cells in all areas of the brain examined; staining was limited to th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.9.5458

    authors: Slater EE,Defendini R,Zimmerman EA

    更新日期:1980-09-01 00:00:00

  • Inducer-mediated commitment of murine erythroleukemia cells to terminal cell division: the expression of commitment.

    abstract::Murine erythroleukemia cells (MELC) are transformed cells that can be induced to differentiate by a variety of agents, such as hexamethylenebisacetamide (HMBA) and dimethyl sulfoxide. Dexamethasone suppresses HMBA-mediated MELC differentiation, but MELC retain a memory for their exposure to HMBA since, on transfer fro...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.11.3394

    authors: Murate T,Kaneda T,Rifkind RA,Marks PA

    更新日期:1984-06-01 00:00:00

  • Real time observation of anaphase in vitro.

    abstract::We used digital fluorescence microscopy to make real-time observations of anaphase chromosome movement and changes in microtubule organization in spindles assembled in Xenopus egg extracts. Anaphase chromosome movement in these extracts resembled that seen in living vertebrate cells. During anaphase chromosomes moved ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.22.12327

    authors: Murray AW,Desai AB,Salmon ED

    更新日期:1996-10-29 00:00:00

  • Desoxyepothilone B is curative against human tumor xenografts that are refractory to paclitaxel.

    abstract::The epothilones are naturally occurring, cytotoxic macrolides that function through a paclitaxel (Taxol)-like mechanism. Although structurally dissimilar, both classes of molecules lead to the arrest of cell division and eventual cell death by stabilizing cellular microtubule assemblies. The epothilones differ in thei...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.26.15798

    authors: Chou TC,Zhang XG,Harris CR,Kuduk SD,Balog A,Savin KA,Bertino JR,Danishefsky SJ

    更新日期:1998-12-22 00:00:00

  • The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

    abstract::d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.150116497

    authors: Roper DI,Huyton T,Vagin A,Dodson G

    更新日期:2000-08-01 00:00:00

  • R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae.

    abstract::Plant cells sense environmental nitrogen levels and alter their gene expression accordingly to survive; however, the underlying regulatory mechanisms still remains to be elucidated. Here, we identified and characterized a transcription factor that is responsible for expression of nitrogen assimilation genes in a unice...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0902790106

    authors: Imamura S,Kanesaki Y,Ohnuma M,Inouye T,Sekine Y,Fujiwara T,Kuroiwa T,Tanaka K

    更新日期:2009-07-28 00:00:00

  • Recombination involving transposable elements: role of target molecule replication in Tn1 delta Ap-mediated replicon fusion.

    abstract::Donor DNA molecules carrying Tn1 or Tn3 deletion mutants do not need to replicate in order to participate in replicon fusion recombination events during which the Tn1/Tn3 element is duplicated. We have assayed Tn1 delta Ap-mediated replicon fusion events involving plasmid R388 and the bacteriophage lambda-derived plas...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.8.2314

    authors: Muster CJ,Shapiro JA,MacHattie LA

    更新日期:1983-04-01 00:00:00

  • Disease-causing missense mutations in actin binding domain 1 of dystrophin induce thermodynamic instability and protein aggregation.

    abstract::Mutations in the dystrophin gene cause Duchenne muscular dystrophy (DMD) most commonly through loss of protein expression. In a small subpopulation of patients, missense mutations can cause DMD, Becker muscular dystrophy, or X-linked cardiomyopathy. Nearly one-half of disease-causing missense mutations are located in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1001517107

    authors: Henderson DM,Lee A,Ervasti JM

    更新日期:2010-05-25 00:00:00

  • Additivity of mutant effects assessed by binomial mutagenesis.

    abstract::Eleven amino acid positions in the helix-turn-helix of lambda repressor have been mutagenized by using a combinatorial method in which alanine is substituted at each position with a probability of 0.5. Approximately 25% of the 2048 proteins in the resulting binomial library are active, including some variants with as ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.9.4246

    authors: Gregoret LM,Sauer RT

    更新日期:1993-05-01 00:00:00

  • Partial similarities between yeast and liver alcohol dehydrogenases.

    abstract::The primary structure of about half of the protein chain of yeast alcohol dehydrogenase has been determined and compared with the amino-acid sequences of other dehydrogenases. The enzyme is found to be distantly related to horse-liver alcohol dehydrogenase, although these two proteins have different quaternary structu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.8.2295

    authors: Jörnvall H

    更新日期:1973-08-01 00:00:00

  • Temperature-compensated circadian clock in the pineal of Anolis.

    abstract::The pineal organ of the lizard Anolis carolinensis can be maintained for up to 10 days in superfused organ culture. During this time it synthesizes and releases melatonin into the medium flowing slowly over it. Collection of timed aliquots of medium and subsequent analysis for melatonin by radioimmunoassay reveal circ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.19.6119

    authors: Menaker M,Wisner S

    更新日期:1983-10-01 00:00:00

  • Imaging of a glioma using peripheral benzodiazepine receptor ligands.

    abstract::Two types of benzodiazepine receptors have been demonstrated in mammalian tissues, one which is localized on neuronal elements in brain and the other, on glial cells and in peripheral tissues such as kidney. In vivo administration of 3H-labeled PK 11195 [1-(2-chlorophenyl-N-methyl-N-(1-methylpropyl)-3-isoquinoline car...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.3.891

    authors: Starosta-Rubinstein S,Ciliax BJ,Penney JB,McKeever P,Young AB

    更新日期:1987-02-01 00:00:00

  • Ca2+ bursts occur around a local minimal concentration of attractant and trigger sperm chemotactic response.

    abstract::Ca(2+) is known to have important roles in sperm chemotaxis, although the relationship between intracellular Ca(2+) concentration ([Ca(2+)](i)) and modulation of the swimming and chemotactic behavior of spermatozoa has not been elucidated. Using a high-speed Ca(2+) imaging system, we examined the chemotactic behavior ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0808580105

    authors: Shiba K,Baba SA,Inoue T,Yoshida M

    更新日期:2008-12-09 00:00:00

  • Maternal intestinal HIF-2α is necessary for sensing iron demands of lactation in mice.

    abstract::The mechanisms that are essential for the maintenance of nutrient status in breast milk are unclear. Our data demonstrate that the intestine via hypoxia-inducible factor (HIF)-2α is an essential regulatory mechanism for maintaining the quality of breast milk. During lactation, intestinal HIF-2α is highly increased, le...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1504891112

    authors: Ramakrishnan SK,Anderson ER,Martin A,Centofanti B,Shah YM

    更新日期:2015-07-14 00:00:00

  • Acetylcholine receptor-alpha-bungarotoxin interactions: determination of the region-to-region contacts by peptide-peptide interactions and molecular modeling of the receptor cavity.

    abstract::In previous studies from this laboratory, the binding regions of alpha-neurotoxins on human and Torpedo acetylcholine (AcCho) receptors (AcChoRs) and the binding regions for the receptor on the toxin were characterized with synthetic peptides of the respective molecules. In the present work, peptides representing the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.16.6156

    authors: Ruan KH,Spurlino J,Quiocho FA,Atassi MZ

    更新日期:1990-08-01 00:00:00

  • Isolation of Drosophila cyclin D, a protein expressed in the morphogenetic furrow before entry into S phase.

    abstract::During Drosophila development, nuclear and cell divisions are coordinated in response to developmental signals. In yeast and mammalian cells, signals that control cell division regulate the activity of cyclin-dependent kinases (Cdks) through proteins such as cyclins that interact with the Cdks. Here we describe two Dr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.7.3011

    authors: Finley RL Jr,Thomas BJ,Zipursky SL,Brent R

    更新日期:1996-04-02 00:00:00

  • A thermogenic fat-epithelium cell axis regulates intestinal disease tolerance.

    abstract::Disease tolerance, the capacity of tissues to withstand damage caused by a stimulus without a decline in host fitness, varies across tissues, environmental conditions, and physiologic states. While disease tolerance is a known strategy of host defense, its role in noninfectious diseases has been understudied. Here, we...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2012003117

    authors: Man K,Bowman C,Braverman KN,Escalante V,Tian Y,Bisanz JE,Ganeshan K,Wang B,Patterson A,Bayrer JR,Turnbaugh PJ,Chawla A

    更新日期:2020-12-15 00:00:00

  • Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes.

    abstract::Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0905469106

    authors: Byun JS,Wong MM,Cui W,Idelman G,Li Q,De Siervi A,Bilke S,Haggerty CM,Player A,Wang YH,Thirman MJ,Kaberlein JJ,Petrovas C,Koup RA,Longo D,Ozato K,Gardner K

    更新日期:2009-11-17 00:00:00

  • C-terminal region of activation-induced cytidine deaminase (AID) is required for efficient class switch recombination and gene conversion.

    abstract::Activation-induced cytidine deaminase (AID) introduces single-strand breaks (SSBs) to initiate class switch recombination (CSR), gene conversion (GC), and somatic hypermutation (SHM). CSR is mediated by double-strand breaks (DSBs) at donor and acceptor switch (S) regions, followed by pairing of DSB ends in two S regio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1324057111

    authors: Sabouri S,Kobayashi M,Begum NA,Xu J,Hirota K,Honjo T

    更新日期:2014-02-11 00:00:00

  • Association of mouse liver adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels with histocompatibility-2 genotype.

    abstract::When the content of cyclic AMP (cAMP) was compared in livers of a series of congenic mouse strains differing at the H-2 locus, significant variation in concentration of cAMP per unit wet weight was found among strains, and also for animals of a given strain with increasing age. For a given age, from 8 to 22 weeks, cAM...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.7.2644

    authors: Meruelo D,Edidin M

    更新日期:1975-07-01 00:00:00

  • UV excitation of single DNA and RNA strands produces high yields of exciplex states between two stacked bases.

    abstract::Excited electronic states created by UV excitation of the diribonucleoside monophosphates ApA, ApG, ApC, ApU, and CpG were studied by the femtosecond transient-absorption technique. Bleach recovery signals recorded at 252 nm show that long-lived excited states are formed in all five dinucleosides. The lifetimes of the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0802079105

    authors: Takaya T,Su C,de La Harpe K,Crespo-Hernández CE,Kohler B

    更新日期:2008-07-29 00:00:00

  • Sex-dependent expression of mouse testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha): cDNA cloning and pretranslational regulation.

    abstract::By using both double-colony hybridization and an in situ immunostaining assay for transformants, 39 cDNA clones (clone p-16 alpha) encoding mouse liver microsomal testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha) were isolated from a cDNA library constructed in the cloning vector pUC-9 with poly(A)+ RNA i...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.7.2024

    authors: Harada N,Negishi M

    更新日期:1985-04-01 00:00:00

  • Synthetic derivatives of polyethyleneimine with enzyme-like catalytic activity (synzymes).

    abstract::A synthetic polymer has been prepared which contains dodecyl groups (to bind small substrate molecules) and methyleneimidazole side chains (as nucleophilic catalytic sites) linked to a polyethyleneimine framework. This macromolecule, with a high local concentration of binding and catalytic groups, catalyzes the hydrol...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.68.2.263

    authors: Klotz IM,Royer GP,Scarpa IS

    更新日期:1971-02-01 00:00:00