Abstract：
:Neurodegenerative disorders can induce modifications of several proteins; one of which is ceruloplasmin (Cp), a ferroxidase enzyme found modified in the cerebrospinal fluid (CSF) of neurodegenerative diseases patients. Cp modifications are caused by the oxidation induced by the pathological environment and are usually associated with activity loss. Together with oxidation, deamidation of Cp was found in the CSF from Alzheimer's and Parkinson's disease patients. Protein deamidation is a process characterized by asparagine residues conversion in either aspartate or isoaspartate, depending on protein sequence/structure and cellular environment. Cp deamidation occurs at two Asparagine-Glycine-Arginine (NGR)-motifs which, once deamidated to isoAspartate-Glycine-Arginine (isoDGR), bind integrins, a family of receptors mediating cell adhesion. Therefore, on the one hand, Cp modifications lead to loss of enzymatic activity, while on the other hand, these alterations confer gain of function to Cp. In fact, deamidated Cp binds to integrins and triggers intracellular signaling on choroid plexus epithelial cells, changing cell functioning. Working in concert with the oxidative environment, Cp deamidation could reach different target cells in the brain, altering their physiology and causing detrimental effects, which might contribute to the pathological mechanism.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zanardi A,Alessio Mdoi
10.3390/ijms22020663subject
Has Abstractpub_date
2021-01-11 00:00:00issue
2issn
1422-0067pii
ijms22020663journal_volume
22pub_type
杂志文章,评审abstract：:Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030758
更新日期：2018-03-07 00:00:00
abstract：:The aim of this study was to determine the effect and mechanism of tamoxifen (TAM)-induced steatosis in vitro. HepG 2 (Human hepatocellular liver carcinoma cell line) cells were treated with different concentrations of TAM for 72 h. Steatosis of hepatocytes was determined after Oil Red O staining and measurement of tr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15034019
更新日期：2014-03-05 00:00:00
abstract：:Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently devel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020323
更新日期：2017-02-04 00:00:00
abstract：:Vulvar squamous cell carcinoma (VSCC) originates from the progression of either a high-grade squamous intraepithelial lesion (HSIL) or differentiated-type vulvar intraepithelial neoplasia (dVIN), often in a background of lichen sclerosus (LS). The mechanisms leading to the progression of these premalignant lesions to ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144880
更新日期：2020-07-10 00:00:00
abstract：:Short-term abiotic stress treatment before harvest can enhance the quality of horticultural crops cultivated in controlled environments. Here, we investigated the effects of combined chilling and UV-A treatment on the accumulation of phenolic compounds in kale (Brassica oleracea var. acephala). Five-week-old plants we...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194950
更新日期：2019-10-08 00:00:00
abstract：:Agricultural production continues to be constrained by a number of biotic and abiotic factors that can reduce crop yield quantity and quality. Potassium (K) is an essential nutrient that affects most of the biochemical and physiological processes that influence plant growth and metabolism. It also contributes to the s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14047370
更新日期：2013-04-02 00:00:00
abstract：:The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has not been completely...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113525
更新日期：2018-11-09 00:00:00
abstract：:Dysregulation of blood sphingolipids is an emerging topic in clinical science. The objective of this study was to determine preanalytical biases that typically occur in clinical and translational studies and that influence measured blood sphingolipid levels. Therefore, we collected blood samples from four healthy male...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19051390
更新日期：2018-05-07 00:00:00
abstract：:Macroautophagy, hereafter referred to as autophagy, is a bulk degradation process performed by lysosomes in which aggregated and altered proteins as well as dysfunctional organelles are decomposed. Autophagy is a basic cellular process that maintains homeostasis and is crucial for postmitotic neurons. Thus, impaired a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161125990
更新日期：2015-11-09 00:00:00
abstract：:Glutamine synthetase 2 (GS2) is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160612713
更新日期：2015-06-04 00:00:00
abstract：:Riboswitches are naturally occurring RNA aptamers that control the expression of essential bacterial genes by binding to specific small molecules. The binding with both high affinity and specificity induces conformational changes. Thus, riboswitches were proposed as a possible molecular target for developing antibioti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21061926
更新日期：2020-03-11 00:00:00
abstract：:Molecular characterization of unsequenced plant species with complex genomes is now possible by genotyping-by-sequencing (GBS) using recent next generation sequencing technologies. This study represents the first use of GBS application to sample genome-wide variants of crested wheatgrass [Agropyron cristatum (L.) Gaer...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092587
更新日期：2018-08-31 00:00:00
abstract：:Thioredoxin fold proteins (TFPs) form a family of diverse proteins involved in thiol/disulfide exchange in cells from all domains of life. Leptospirillum spp. are bioleaching bacteria naturally exposed to extreme conditions like acidic pH and high concentrations of metals that can contribute to the generation of react...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051880
更新日期：2020-03-10 00:00:00
abstract：:The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studies depends on the approaches used to account for non-biological variations. In order to find a proper normalization strategy for the study of genes related to growth hormone signaling in skeletal muscle of growing mice,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051060
更新日期：2017-05-16 00:00:00
abstract：:Ataxia is a common clinical feature in inherited metabolic disorders. There are more than 150 inherited metabolic disorders in patients presenting with ataxia in addition to global developmental delay, encephalopathy episodes, a history of developmental regression, coarse facial features, seizures, and other types of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155519
更新日期：2020-08-01 00:00:00
abstract：:Osteosarcoma (OS) is the most common type of primary bone tumor. Currently, there are limited treatment options for metastatic OS. Alpha-ketoglutarate (AKG), i.e., a multifunctional intermediate of the Krebs cycle, is one of the central metabolic regulators of tumor fate and plays an important role in cancerogenesis a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249406
更新日期：2020-12-10 00:00:00
abstract：:Patients suffering from liver cirrhosis are often complicated with the formation of portosystemic collateral vessels, which is associated with the progression of a splanchnic hyperdynamic circulatory state. Alleviating pathological angiogenesis has thus been proposed to be a feasible treatment strategy. Indole-3-carbi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174161
更新日期：2019-08-26 00:00:00
abstract：:Viral persistency in latently infected CD4+ T cells despite antiretroviral therapy (ART) represents a major drawback in the fight against HIV-1. Efforts to purge latent HIV-1 have been attempted using latency reversing agents (LRAs) that activate expression of the quiescent virus. However, initial trials have shown th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20184490
更新日期：2019-09-11 00:00:00
abstract：:Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Phy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041417
更新日期：2020-02-19 00:00:00
abstract：:When Clostridium tetani was discovered and identified as a Gram-positive anaerobic bacterium of the genus Clostridium, the possibility of turning its toxin into a valuable biological carrier to ameliorate neurodegenerative processes was inconceivable. However, the non-toxic carboxy-terminal fragment of the tetanus tox...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms13066883
更新日期：2012-01-01 00:00:00
abstract：:Two mesenchymal zinc transporters, ZIP7 and ZIP13, play critical roles in dermal development. ZIP7 and ZIP13 are the closest among the conserved mammalian zinc transporters. However, whether their functions are complementary remains a controversial issue. In the present study, we found that the expression of ZIP13, bu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20163941
更新日期：2019-08-13 00:00:00
abstract：:Human Ribonuclease 6 is a secreted protein belonging to the ribonuclease A (RNaseA) superfamily, a vertebrate specific family suggested to arise with an ancestral host defense role. Tissue distribution analysis revealed its expression in innate cell types, showing abundance in monocytes and neutrophils. Recent evidenc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17040552
更新日期：2016-04-13 00:00:00
abstract：:Transcription factors (TFs) play essential roles in the transcriptional regulation of functional genes, and are involved in diverse physiological processes in living organisms. The fruit fly Drosophila melanogaster, a simple and easily manipulated organismal model, has been extensively applied to study the biological ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19113691
更新日期：2018-11-21 00:00:00
abstract：:Several studies have demonstrated that basic fibroblast growth factor (bFGF) can induce neural differentiation of mesenchymal stem cells. In this study, we investigated the neural differentiation of muscle-derived stem cells (MDSCs) following treatment with bFGF and ethosuximide, a small molecule used as an anticonvul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14046614
更新日期：2013-03-25 00:00:00
abstract：:Brassinosteroids (BRs) are a class of phytohormones, which regulate various processes during plant life cycle. Intensive studies conducted with genetic, physiological and molecular approaches allowed identification of various components participating in the BR signaling-from the ligand perception, through cytoplasmic ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19092675
更新日期：2018-09-09 00:00:00
abstract：:Here is presented an investigation of the use of normal modes in protein-protein docking, both in theory and in practice. Upper limits of the ability of normal modes to capture the unbound to bound conformational change are calculated on a large test set, with particular focus on the binding interface, the subset of r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11103623
更新日期：2010-09-28 00:00:00
abstract：:The effects of various inhibitors on crude, commercial and partially purified commercial mushroom tyrosinase were examined by comparing IC(50) values. Kojic acid, salicylhydroxamic acid, tropolone, methimazole, and ammonium tetrathiomolybdate had relatively similar IC(50) values for the crude, commercial and partially...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10093811
更新日期：2009-09-02 00:00:00
abstract：:Verticillium dahliae (V. dahliae) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by knocking out the corresponding VdTHI20...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041378
更新日期：2020-02-18 00:00:00
abstract：:Frizzled receptors (FZDs) are a family of seven-span transmembrane receptors with hallmarks of G protein-coupled receptors (GPCRs) that serve as receptors for secreted Wingless-type (WNT) ligands in the WNT signaling pathway. Functionally, FZDs play crucial roles in regulating cell polarity, embryonic development, cel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19051543
更新日期：2018-05-22 00:00:00
abstract：:Due to the present scenario of climate change, plants have to evolve strategies to survive and perform under a plethora of biotic and abiotic stresses, which restrict plant productivity. Maintenance of plant protein functional conformation and preventing non-native proteins from aggregation, which leads to metabolic d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215321
更新日期：2019-10-25 00:00:00
