Abstract:
:Glutamine synthetase 2 (GS2) is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate the chlorosis mechanism, we systematically analyzed the plant growth, carbon-nitrogen metabolism and gene expressions between the GS2-cosuppressed rice and wild-type plants. The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems. Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage. The analysis of the metabolic profile showed a significant increase of sugars and organic acids. Additionally, gene expression patterns were different in root and leaf of GS2-cosuppressed plants between the seedling and tillering stage. These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Bao A,Zhao Z,Ding G,Shi L,Xu F,Cai Hdoi
10.3390/ijms160612713subject
Has Abstractpub_date
2015-06-04 00:00:00pages
12713-36issue
6issn
1422-0067pii
ijms160612713journal_volume
16pub_type
杂志文章abstract::Oxidative stress is considered to be a key factor of the pathogenesis of Parkinson's disease, a multifactorial neurodegenerative disorder characterized by reduced dopaminergic neurons in the substantia nigra pars compacta and accumulated protein aggregates. Rotenone is a worldwide-used pesticide that induces the most ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082761
更新日期:2020-04-16 00:00:00
abstract:BACKGROUND:Nowadays, the molecular mechanisms governing TSD (temperature-dependent sex determination) or GSD + TE (genotypic sex determination + temperature effects) remain a mystery in fish. METHODS:We developed three all-female families of Nile tilapia (Oreochromis niloticus), and the family with the highest male ra...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030689
更新日期:2018-02-28 00:00:00
abstract::Spinocerebellar ataxia type 3 (SCA3) is a devastating neurodegenerative disease for which there is currently no cure, nor effective treatment strategy. One of nine polyglutamine disorders known to date, SCA3 is clinically heterogeneous and the main feature is progressive ataxia, which in turn affects speech, balance a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215434
更新日期:2019-10-31 00:00:00
abstract::Integration of multiple sources and data levels provides a great insight into the complex associations between human and malaria systems. In this study, a meta-analysis framework was developed based on a heterogeneous network model for integrating human-malaria protein similarities, a human protein interaction network...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041310
更新日期:2020-02-15 00:00:00
abstract::Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249380
更新日期:2020-12-09 00:00:00
abstract::As a response to environmental changes driven by the Earth's axial rotation, most organisms evolved an internal biological timer-the so called circadian clock-which regulates physiology and behavior in a rhythmic fashion. Emerging evidence suggests an intimate interplay between the circadian clock and another fundamen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18040873
更新日期:2017-04-20 00:00:00
abstract::Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218354
更新日期:2020-11-07 00:00:00
abstract::Spinal cord injury is a chronic and debilitating neurological condition that is currently being managed symptomatically with no real therapeutic strategies available. Even though there is no consensus on the best time to start interventions, the chronic phase is definitely the most stable target in order to determine ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19061776
更新日期:2018-06-15 00:00:00
abstract::Sabicea species are used in the Amazon for treatment of fever and malaria, which suggests that its chemical constituents may have some effect on pain and inflammation. Phytochemical analysis of the hexane fraction obtained from the crude ethanol extract from Sabicea grisea var. grisea Cham. & Schltdl (Rubiaceae), an e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13021598
更新日期:2012-01-01 00:00:00
abstract::Understanding molecular interactions and regulatory mechanisms in tumor initiation, progression, and treatment response are key requirements towards advanced cancer diagnosis and novel treatment procedures in personalized medicine. Beyond decoding the gene expression, malfunctioning and cancer-related epigenetic pathw...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18102066
更新日期:2017-09-28 00:00:00
abstract::In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. He...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112348
更新日期:2017-11-07 00:00:00
abstract::Tuberculosis (TB) is a major health threat to the human population worldwide. The etiology of the disease is Mycobacterium tuberculosis (Mtb), a highly successful intracellular pathogen. It has the ability to manipulate the host immune response and to make the intracellular environment suitable for its survival. Many ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122725
更新日期:2017-12-16 00:00:00
abstract::Prostate cancer is the most prevalent disease affecting males in many Western countries, with an estimated 29,480 deaths in 2014 in the US alone. Incidence rates for prostate cancer deaths have been decreasing since the early 1990s in men of all races/ethnicities, though they remain about 60% higher in African America...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms16023350
更新日期:2015-02-03 00:00:00
abstract::Autoimmune diabetes is a complex multifactorial disease with genetic and environmental factors playing pivotal roles. While many genes associated with the risk of diabetes have been identified to date, the mechanisms by which external triggers contribute to the genetic predisposition remain unclear. Here, we derived e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165743
更新日期:2020-08-11 00:00:00
abstract::Impaired NO-cGMP signaling has been linked to several neurological disorders. NO-sensitive guanylyl cyclase (NO-GC), of which two isoforms-NO-GC1 and NO-GC2-are known, represents a promising drug target to increase cGMP in the brain. Drug-like small molecules have been discovered that work synergistically with NO to s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082313
更新日期:2018-08-07 00:00:00
abstract:INTRODUCTION:Targeted agents such as bevacizumab (BEV) or poly (ADP-ribose) polymerase inhibitors (PARPi) which have been added as concomitant or maintenance therapies have been shown to improve progression-free survival (PFS) in patients with platinum-sensitive recurrent ovarian cancer (PS rOC). In the absence of dire...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113805
更新日期:2020-05-27 00:00:00
abstract::Psoriasis is a chronic inflammatory skin disease characterized by excessive growth of keratinocytes and hyperkeratosis in the epidermis. An abnormality of the non-lesional epidermis at an early stage of psoriasis is involved in triggering inflammatory cell infiltration into the dermis. Integrin α5β1 acts as a receptor...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20133144
更新日期:2019-06-27 00:00:00
abstract::Despite the critical role of melanin in the protection of skin against UV radiation, excess production of melanin can lead to hyperpigmentation and skin cancer. Pear fruits are often used in traditional medicine for the treatment of melasma; therefore, we investigated the effects of pear extract (PE) and its component...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081809
更新日期:2017-08-21 00:00:00
abstract::The objectives of this study were to evaluate the binding behavior of Cu(II) and Eu(III) with water extractable organic matter (WEOM) in soil, and assess the competitive effect of the cations. Excitation-emission matrix (EEM) fluorescence spectrometry was used in combination with parallel factor analysis (PARAFAC) to ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160714464
更新日期:2015-06-25 00:00:00
abstract::The core of micelles self-assembled from amphiphiles is hydrophobic and contains little water, whereas complex coacervate core micelles co-assembled from oppositely charged hydrophilic polymers have a hydrophilic core with a high water content. Co-assembly of ionic surfactants with ionic-neutral copolymers yields surf...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010337
更新日期:2020-12-30 00:00:00
abstract::The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity wa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12053366
更新日期:2011-01-01 00:00:00
abstract::Carbamazepine (CBZ) binds adenosine receptors, but detailed effects of CBZ on astroglial transmission associated with adenosine receptor still need to be clarified. To clarify adenosinergic action of CBZ on astroglial transmission, primary cultured astrocytes were acutely or chronically treated with CBZ, proinflammato...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20153727
更新日期:2019-07-30 00:00:00
abstract::Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure-function relationships. However, the study of integral membrane proteins is still in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20102605
更新日期:2019-05-27 00:00:00
abstract::Sleep-wake disorders are highly prevalent disorders, which can lead to negative effects on cognitive, emotional and interpersonal functioning, and can cause maladaptive metabolic changes. Recent studies support the notion that metabolic processes correlate with sleep. The study of metabolite biomarkers (metabolomics) ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197244
更新日期:2020-09-30 00:00:00
abstract::The Gram-negative Campylobacter jejuni is a major cause of foodborne gastroenteritis in humans worldwide. The cytotoxic effects of Campylobacter have been mainly ascribed to the actions of the cytolethal distending toxin (CDT): it is mandatory to put in evidence risk factors for sequela development, such as reactive a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062207
更新日期:2020-03-23 00:00:00
abstract::The adsorption of amyloidogenic peptides, amyloid beta 1-40 (Aβ1-40), alpha-synuclein (α-syn), and beta 2 microglobulin (β2m), was attempted over the surface of nano-gold colloidal particles, ranging from d = 10 to 100 nm in diameter (d). The spectroscopic inspection between pH 2 and pH 12 successfully extracted the c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215354
更新日期:2019-10-28 00:00:00
abstract::Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype's chief characteristics are dep...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249676
更新日期:2020-12-18 00:00:00
abstract::Vulvar squamous cell carcinoma (VSCC) originates from the progression of either a high-grade squamous intraepithelial lesion (HSIL) or differentiated-type vulvar intraepithelial neoplasia (dVIN), often in a background of lichen sclerosus (LS). The mechanisms leading to the progression of these premalignant lesions to ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144880
更新日期:2020-07-10 00:00:00
abstract::In this issue, we are pleased and honored to have an interview with Professor Martin Pichler, who is the Collection Editor for the International Journal of Molecular Sciences Topical Collection of "Regulation by Non-Coding RNAs" [1].[...]. ...
journal_title:International journal of molecular sciences
pub_type: 社论,面试
doi:10.3390/ijms17040605
更新日期:2016-04-21 00:00:00
abstract::Neurodegenerative disorders can induce modifications of several proteins; one of which is ceruloplasmin (Cp), a ferroxidase enzyme found modified in the cerebrospinal fluid (CSF) of neurodegenerative diseases patients. Cp modifications are caused by the oxidation induced by the pathological environment and are usually...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020663
更新日期:2021-01-11 00:00:00