Abstract：
:Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure-function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional protein are always required for biochemical and structural studies. Many modern biophysical tools are available today that provide critical information regarding to the characterisation and behaviour of integral membrane proteins in solution. These biophysical approaches play an important role in both basic research and in early-stage drug discovery processes. In this review, it is not our objective to present a comprehensive list of all existing biophysical methods, but a selection of the most useful and easily applied to basic integral membrane protein research.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kwan TOC,Reis R,Siligardi G,Hussain R,Cheruvara H,Moraes Idoi
10.3390/ijms20102605subject
Has Abstractpub_date
2019-05-27 00:00:00issue
10issn
1422-0067pii
ijms20102605journal_volume
20pub_type
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