Abstract：
:Molecular characterization of unsequenced plant species with complex genomes is now possible by genotyping-by-sequencing (GBS) using recent next generation sequencing technologies. This study represents the first use of GBS application to sample genome-wide variants of crested wheatgrass [Agropyron cristatum (L.) Gaertn.] and assess the genetic diversity present in 192 genotypes from 12 tetraploid lines. Bioinformatic analysis identified 45,507 single nucleotide polymorphism (SNP) markers in this outcrossing grass species. The model-based Bayesian analysis revealed four major clusters of the samples assayed. The diversity analysis revealed 15.8% of SNP variation residing among the 12 lines, and 12.1% SNP variation present among four genetic clusters identified by the Bayesian analysis. The principal coordinates analysis and dendrogram were able to distinguish four lines of Asian origin from Canadian cultivars and breeding lines. These results serve as a valuable resource for understanding genetic variability, and will aid in the genetic improvement of this outcrossing polyploid grass species for forage production. These findings illustrate the potential of GBS application in the characterization of non-model polyploid plants with complex genomes.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Baral K,Coulman B,Biligetu B,Fu YBdoi
10.3390/ijms19092587subject
Has Abstractpub_date
2018-08-31 00:00:00issue
9issn
1422-0067pii
ijms19092587journal_volume
19pub_type
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2017-11-15 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-07-26 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-03-21 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-04-03 00:00:00
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journal_title：International journal of molecular sciences
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abstract：:Our previous study showed that a single lipopolysaccharide (LPS) treatment to neonatal rats could induce a long-lasting neuroinflammatory response and dopaminergic system injury late in life. This is evidenced by a sustained activation of microglia and elevated interleukin-1β (IL-1β) levels, as well as reduced tyrosin...
journal_title：International journal of molecular sciences
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更新日期：2015-04-17 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2019-08-12 00:00:00
abstract：:Homologous recombination (HR) is often used to achieve targeted gene integration because of its higher precision and operability compared with microhomology-mediated end-joining (MMEJ) or non-homologous end-joining (NHEJ). It appears to be inefficient for gene integration in animal cells and embryos due to occurring o...
journal_title：International journal of molecular sciences
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更新日期：2020-08-13 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2016-11-01 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2018-01-11 00:00:00
abstract：BACKGROUND:EphrinA1-Fc abolishes acute I/R injury and attenuates nonreperfused cardiac injury 4 days after permanent occlusion in mice. The goal of this study was to assess the capacity of a single intramyocardial administration of ephrinA1-Fc at the time of coronary artery ligation, to determine the degree to which ea...
journal_title：International journal of molecular sciences
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更新日期：2020-08-13 00:00:00
