BingleSeq: a user-friendly R package for bulk and single-cell RNA-Seq data analysis.

Abstract:

Background:RNA sequencing is an indispensable research tool used in a broad range of transcriptome analysis studies. The most common application of RNA Sequencing is differential expression analysis and it is used to determine genetic loci with distinct expression across different conditions. An emerging field called single-cell RNA sequencing is used for transcriptome profiling at the individual cell level. The standard protocols for both of these approaches include the processing of sequencing libraries and result in the generation of count matrices. An obstacle to these analyses and the acquisition of meaningful results is that they require programing expertise. Although some effort has been directed toward the development of user-friendly RNA-Seq analysis analysis tools, few have the flexibility to explore both Bulk and single-cell RNA sequencing. Implementation:BingleSeq was developed as an intuitive application that provides a user-friendly solution for the analysis of count matrices produced by both Bulk and Single-cell RNA-Seq experiments. This was achieved by building an interactive dashboard-like user interface which incorporates three state-of-the-art software packages for each type of the aforementioned analyses. Furthermore, BingleSeq includes additional features such as visualization techniques, extensive functional annotation analysis and rank-based consensus for differential gene analysis results. As a result, BingleSeq puts some of the best reviewed and most widely used packages and tools for RNA-Seq analyses at the fingertips of biologists with no programing experience. Availability:BingleSeq is as an easy-to-install R package available on GitHub at https://github.com/dbdimitrov/BingleSeq/.

journal_name

PeerJ

journal_title

PeerJ

authors

Dimitrov D,Gu Q

doi

10.7717/peerj.10469

subject

Has Abstract

pub_date

2020-12-22 00:00:00

pages

e10469

issn

2167-8359

pii

10469

journal_volume

8

pub_type

杂志文章

相关文献

PeerJ文献大全
  • Correction of estimation bias of predictive equations of energy expenditure based on wrist/waist-mounted accelerometers.

    abstract:Background:Using wearable inertial sensors to accurately estimate energy expenditure (EE) during an athletic training process is important. Due to the characteristics of inertial sensors, however, the positions in which they are worn can produce signals of different natures. To understand and solve this issue, this stu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7973

    authors: Ho CS,Chang CH,Lin KC,Huang CC,Hsu YJ

    更新日期:2019-11-04 00:00:00

  • Using 3D printed eggs to examine the egg-rejection behaviour of wild birds.

    abstract::The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and p...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.965

    authors: Igic B,Nunez V,Voss HU,Croston R,Aidala Z,López AV,Van Tatenhove A,Holford ME,Shawkey MD,Hauber ME

    更新日期:2015-05-26 00:00:00

  • The advanced lung cancer inflammation index predicts short and long-term outcomes in patients with colorectal cancer following surgical resection: a retrospective study.

    abstract:Background and Purpose:Several studies have proposed that the advanced lung cancer inflammation index (ALI), a new inflammation-related index, can be used for the prognosis assessment of various malignancies. However, few studies have reported its prognostic value in colorectal cancer (CRC). Therefore, this study explo...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10100

    authors: Xie H,Huang S,Yuan G,Kuang J,Yan L,Wei L,Tang S,Gan J

    更新日期:2020-10-08 00:00:00

  • A comprehensive investigation of mesophotic coral ecosystems in the Hawaiian Archipelago.

    abstract::Although the existence of coral-reef habitats at depths to 165 m in tropical regions has been known for decades, the richness, diversity, and ecological importance of mesophotic coral ecosystems (MCEs) has only recently become widely acknowledged. During an interdisciplinary effort spanning more than two decades, we c...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2475

    authors: Pyle RL,Boland R,Bolick H,Bowen BW,Bradley CJ,Kane C,Kosaki RK,Langston R,Longenecker K,Montgomery A,Parrish FA,Popp BN,Rooney J,Smith CM,Wagner D,Spalding HL

    更新日期:2016-10-04 00:00:00

  • Taxonomic richness and abundance of cryptic peracarid crustaceans in the Puerto Morelos Reef National Park, Mexico.

    abstract:BACKGROUND AND AIMS:Cryptic peracarids are an important component of the coral reef fauna in terms of diversity and abundance, yet they have been poorly studied. The aim of this study was to evaluate the taxonomic richness and abundance of cryptic peracarids in coral rubble in the Puerto Morelos Reef National Park, Mex...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3411

    authors: Monroy-Velázquez LV,Rodríguez-Martínez RE,Alvarez F

    更新日期:2017-06-15 00:00:00

  • Long-term administration of vitamin B12 and adenosine triphosphate for idiopathic sudden sensorineural hearing loss: a retrospective study.

    abstract:Background:In idiopathic sudden sensorineural hearing loss (ISSNHL), the relationship between the administration duration of vitamin B12 (vit B12) with adenosine triphosphate (ATP) and their therapeutic effect is not fully understood. Objective:To investigate the therapeutic effect of long-term 16 (≥weeks) administrat...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10406

    authors: Kurioka T,Sano H,Furuki S,Yamashita T

    更新日期:2020-12-16 00:00:00

  • The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development.

    abstract::The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3390

    authors: Kostrouchová M,Kostrouch D,Chughtai AA,Kaššák F,Novotný JP,Kostrouchová V,Benda A,Krause MW,Saudek V,Kostrouchová M,Kostrouch Z

    更新日期:2017-06-06 00:00:00

  • Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos.

    abstract:Background and Purpose:Oxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of R. coriaria sumac fruit extract on human myoblasts and zebrafish embryos in condition...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4144

    authors: Najjar F,Rizk F,Carnac G,Nassar R,Jabak S,Sobolev AP,Bou Saada Y,El Sabban M,Hamade A

    更新日期:2017-12-12 00:00:00

  • Evaluation of ddRADseq for reduced representation metagenome sequencing.

    abstract:BACKGROUND:Profiling of microbial communities via metagenomic shotgun sequencing has enabled researches to gain unprecedented insight into microbial community structure and the functional roles of community members. This study describes a method and basic analysis for a metagenomic adaptation of the double digest restr...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3837

    authors: Liu MY,Worden P,Monahan LG,DeMaere MZ,Burke CM,Djordjevic SP,Charles IG,Darling AE

    更新日期:2017-09-19 00:00:00

  • Identification of a native Bacillus thuringiensis strain from Sri Lanka active against Dipel-resistant Plutella xylostella.

    abstract:Background:Biopesticides based on strains of the bacterium Bacillus thuringiensis (Bt) are used globally for effective and environmentally friendly pest control. The most serious threat to the sustainable use of these microbial pesticides is the development of resistance on targeted pests. Populations of Plutella xylos...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7535

    authors: Baragamaarachchi RY,Samarasekera JKRR,Weerasena OVDSJ,Lamour K,Jurat-Fuentes JL

    更新日期:2019-08-23 00:00:00

  • Historical reconstruction of climatic and elevation preferences and the evolution of cloud forest-adapted tree ferns in Mesoamerica.

    abstract:BACKGROUND:Cloud forests, characterized by a persistent, frequent or seasonal low-level cloud cover and fragmented distribution, are one of the most threatened habitats, especially in the Neotropics. Tree ferns are among the most conspicuous elements in these forests, and ferns are restricted to regions in which minimu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2696

    authors: Sosa V,Ornelas JF,Ramírez-Barahona S,Gándara E

    更新日期:2016-11-16 00:00:00

  • Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash.

    abstract::Background. Two non-tuberculous mycobacterial strains, UM_3 and UM_11, were isolated from the trunk wash of captive elephants in Malaysia. As they appeared to be identical phenotypes, they were investigated further by conventional and whole genome sequence-based methods of strain differentiation. Methods. Multiphasic ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1367

    authors: Chan KG,Loke MF,Ong BL,Wong YL,Hong KW,Tan KH,Kaur S,Ng HF,Abdul Razak M,Ngeow YF

    更新日期:2015-11-10 00:00:00

  • A collection of hexactinellids (Porifera) from the deep South Atlantic and North Pacific: new genus, new species and new records.

    abstract::This article describes or redescribes four hexactinellid sponges, namely Poliopogon amadou, Euplectella sanctipauli sp. nov., Bolosoma perezi sp. nov. and Advhena magnifica gen. et sp. nov. P. amadou, E. sanctipauli sp. nov. and B. perezi sp. nov. represent new findings for the South Atlantic deep-sea fauna, including...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9431

    authors: Castello-Branco C,Collins AG,Hajdu E

    更新日期:2020-07-09 00:00:00

  • Histological analysis of post-eruption tooth wear adaptations, and ontogenetic changes in tooth implantation in the acrodontan squamate Pogona vitticeps.

    abstract::Teeth have been a focus of research in both extinct and extant taxa alike; a significant portion of dental literature is concerned with dental patterning and replacement. Most non-mammalian vertebrates continuously replace their dentition but an anomalous group of squamates has forgone this process in only having one ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5923

    authors: Haridy Y

    更新日期:2018-11-08 00:00:00

  • Stability of commercial glucanase and β-glucosidase preparations under hydrolysis conditions.

    abstract::The cost of enzymes makes enzymatic hydrolysis one of the most expensive steps in the production of lignocellulosic ethanol. Diverse studies have used commercial enzyme cocktails assuming that change in total protein concentration during hydrolysis was solely due to adsorption of endo- and exoglucanases onto the subst...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.402

    authors: Rosales-Calderon O,Trajano HL,Duff SJ

    更新日期:2014-06-10 00:00:00

  • Taxonomic reassessment of the genus Dichotomius (Coleoptera: Scarabaeinae) through integrative taxonomy.

    abstract::Dung beetles of the subfamily Scarabaeinae are widely recognised as important providers of multiple ecosystem services and are currently experiencing revisions that have improved our understanding of higher-level relationships in the subfamily. However, the study of phylogenetic relationships at the level of genus or ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7332

    authors: Pardo-Diaz C,Lopera Toro A,Peña Tovar SA,Sarmiento-Garcés R,Sanchez Herrera M,Salazar C

    更新日期:2019-08-05 00:00:00

  • Why the long face? Comparative shape analysis of miniature, pony, and other horse skulls reveals changes in ontogenetic growth.

    abstract:Background:Much of the shape variation found in animals is based on allometry and heterochrony. Horses represent an excellent model to investigate patterns of size-shape variation among breeds that were intentionally bred for extreme small and large sizes. Methods:We tested whether ponies (wither height < 148 cm) have...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7678

    authors: Heck L,Sanchez-Villagra MR,Stange M

    更新日期:2019-09-16 00:00:00

  • Short-term starvation at low temperature prior to harvest does not impact the health and acute stress response of adult Atlantic salmon.

    abstract::A period of starvation is regarded as a sound practice in aquaculture prior to handling, transportation and harvest, to minimise impacts on welfare and ensure proper hygiene after harvest. However, documentation of welfare issues such as stress following starvation and handling in adult Atlantic salmon are lacking. Th...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3273

    authors: Waagbø R,Jørgensen SM,Timmerhaus G,Breck O,Olsvik PA

    更新日期:2017-04-27 00:00:00

  • N-acetylcysteine protects against motor, optomotor and morphological deficits induced by 6-OHDA in zebrafish larvae.

    abstract:Background:Parkinson's disease (PD) is the second most common neurodegenerative disorder. In addition to its highly debilitating motor symptoms, non-motor symptoms may precede their motor counterparts by many years, which may characterize a prodromal phase of PD. A potential pharmacological strategy is to introduce neu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4957

    authors: Benvenutti R,Marcon M,Reis CG,Nery LR,Miguel C,Herrmann AP,Vianna MRM,Piato A

    更新日期:2018-06-01 00:00:00

  • Identification and prognostic value of DLGAP5 in endometrial cancer.

    abstract:Background:Endometrial cancer poses a serious threat to women's health worldwide, and its pathogenesis, although actively explored, is not fully understood. DLGAP5 is a recently identified cell cycle-regulation gene not reported in endometrial cancer. This study was aiming to analyze the role of DLGAP5 in tumorigenesis...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10433

    authors: Zheng R,Shi Z,Li W,Yu J,Wang Y,Zhou Q

    更新日期:2020-11-27 00:00:00

  • 3D skeletal muscle fascicle engineering is improved with TGF-β1 treatment of myogenic cells and their co-culture with myofibroblasts.

    abstract:Background:Skeletal muscle wound healing is dependent on complex interactions between fibroblasts, myofibroblasts, myogenic cells, and cytokines, such as TGF-β1. This study sought to clarify the impact of TGF-β1 signaling on skeletal muscle cells and discern between the individual contributions of fibroblasts and myofi...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4939

    authors: Krieger J,Park BW,Lambert CR,Malcuit C

    更新日期:2018-07-11 00:00:00

  • Geriatric syndromes are potential determinants of the medication adherence status in prevalent dialysis patients.

    abstract::Background. Geriatric syndromes (GS) exhibit high prevalence in patients with end-stage renal disease (ESRD) under chronic dialysis irrespective of age. We sought to determine whether GS influences medication adherence in ESRD patients. Methods. A prospective cohort of chronic dialysis patients was assembled. The pres...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2122

    authors: Chao CT,Huang JW,COGENT (COhort of GEriatric Nephrology in NTUH) study group.

    更新日期:2016-06-14 00:00:00

  • Single cell transcriptional analysis reveals novel innate immune cell types.

    abstract::Single-cell analysis has the potential to provide us with a host of new knowledge about biological systems, but it comes with the challenge of correctly interpreting the biological information. While emerging techniques have made it possible to measure inter-cellular variability at the transcriptome level, no consensu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.452

    authors: Kippner LE,Kim J,Gibson G,Kemp ML

    更新日期:2014-06-24 00:00:00

  • Gene buddies: linked balanced polymorphisms reinforce each other even in the absence of epistasis.

    abstract::The fates of genetic polymorphisms maintained by balancing selection depend on evolutionary dynamics at linked sites. While coevolution across linked, epigenetically-interacting loci has been extensively explored, such supergenes may be relatively rare. However, genes harboring adaptive variation can occur in close ph...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5110

    authors: Tennessen JA

    更新日期:2018-06-28 00:00:00

  • BioWorkbench: a high-performance framework for managing and analyzing bioinformatics experiments.

    abstract::Advances in sequencing techniques have led to exponential growth in biological data, demanding the development of large-scale bioinformatics experiments. Because these experiments are computation- and data-intensive, they require high-performance computing techniques and can benefit from specialized technologies such ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5551

    authors: Mondelli ML,Magalhães T,Loss G,Wilde M,Foster I,Mattoso M,Katz D,Barbosa H,de Vasconcelos ATR,Ocaña K,Gadelha LMR Jr

    更新日期:2018-08-29 00:00:00

  • A new species of Atrimitra Dall, 1918 (Gastropoda: Mitridae) from seamounts of the recently created Nazca-Desventuradas Marine Park, Chile.

    abstract::We describe Atrimitra isolata sp. n. (Gastropoda: Mitridae), collected on the summit of seamounts (~200 m water depth) in the vicinity of Desventuradas Islands, Chile insular territory. Additionally, we provide some insight into the habitat of this new species based on underwater imagery taken with a remotely operated...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8279

    authors: Sellanes J,Salisbury RA,Tapia JM,Asorey CM

    更新日期:2019-12-20 00:00:00

  • Widespread mitovirus sequences in plant genomes.

    abstract::The exploration of the evolution of RNA viruses has been aided recently by the discovery of copies of fragments or complete genomes of non-retroviral RNA viruses (Non-retroviral Endogenous RNA Viral Elements, or NERVEs) in many eukaryotic nuclear genomes. Among the most prominent NERVEs are partial copies of the RNA d...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.876

    authors: Bruenn JA,Warner BE,Yerramsetty P

    更新日期:2015-04-09 00:00:00

  • Parents know best: transgenerational predator recognition through parental effects.

    abstract::In highly biodiverse systems, such as coral reefs, prey species are faced with predatory threats from numerous species. Recognition of predators can be innate, or learned, and can help increase the chance of survival. Research suggests that parental exposure to increased predatory threats can affect the development, b...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9340

    authors: Atherton JA,McCormick MI

    更新日期:2020-06-18 00:00:00

  • In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation.

    abstract::One of the best ex situ conservation strategies for wild germplasm is in vitro conservation of genetic banks. The success of in vitro conservation relies heavily on the micropropagation or performance of the species of interest. In the context of global change, crop production challenges and climate change, we face a ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7017

    authors: Hernández-Terán A,Wegier A,Benítez M,Lira R,Sosa Fuentes TG,Escalante AE

    更新日期:2019-06-10 00:00:00

  • Biodiversity of carapace epibiont diatoms in loggerhead sea turtles (Caretta caretta Linnaeus 1758) in the Aegean Sea Turkish coast.

    abstract:Background:The Aegean Sea coast of Turkey hosts one of the most important nesting grounds for loggerhead sea turtles (Caretta caretta) in the Mediterranean Sea. Previous studies have revealed that the sea turtle carapace provides favourable conditions for various epibiontic organisms. Epibionts occurring on the carapac...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9406

    authors: Kaleli A,Car A,Witkowski A,Krzywda M,Riaux-Gobin C,Solak CN,Kaska Y,Zgłobicka I,Płociński T,Wróbel R,Kurzydłowski K

    更新日期:2020-07-17 00:00:00