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Cell-free Stem Cell-Derived Extract Formulation for Regenerative Medicine Applications.

Abstract：

:Stem cells for regenerative medicine purposes offer therapeutic benefits, but disadvantages are still ill defined. The benefit of stem cells may be attributed to their secretion of growth factors (GFs), cytokines (CKs), and extracellular vesicles (EVs), including exosomes. We present a novel cell-free stem cell-derived extract (CCM), formulated from human progenitor endothelial stem cells (hPESCs), characterized for biologically active factors using ELISA, nanoparticle tracking analysis and single particle interferometric reflectance imaging sensing. The effect on fibroblast proliferation and ability to induce stem cell migration was analyzed using Alamar Blue proliferation and Transwell migration assays, respectively. GFs including IGFBP 1, 2, 3, and 6, insulin, growth hormone, PDGF-AA, TGF-α, TGF-β1, VEGF, and the anti-inflammatory cytokine, IL-1RA were detected. Membrane enclosed particles within exosome size range and expressing exosome tetraspanins CD81 and CD9 were identified. CCM significantly increased cell proliferation and induced stem cell migration. Analysis of CCM revealed presence of GFs, CKs, and EVs, including exosomes. The presence of multiple factors including exosomes within one formulation, the ability to promote cell proliferation and induce stem cell migration may reduce inflammation and pain, and augment tissue repair.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Gupta A,Cady C,Fauser AM,Rodriguez HC,Mistovich RJ,Potty AGR,Maffulli N

doi

10.3390/ijms21249364

subject

Has Abstract

pub_date

2020-12-09 00:00:00

issue

24

issn

1422-0067

pii

ijms21249364

journal_volume

21

pub_type

杂志文章

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