Abstract:
:Stem cells harbor significant potential for regenerative medicine as well as basic and clinical translational research. Prior to harnessing their reparative nature for degenerative diseases, concerns regarding their genetic integrity and mutation acquisition need to be addressed. Here we review pluripotent and multipotent stem cell response to DNA damage including differences in DNA repair kinetics, specific repair pathways (homologous recombination vs. non-homologous end joining), and apoptotic sensitivity. We also describe DNA damage and repair strategies during reprogramming and discuss potential genotoxic agents that can reduce the inherent risk for teratoma formation and mutation accumulation. Ensuring genomic stability in stem cell lines is required to achieve the quality control standards for safe clinical application.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wyles SP,Brandt EB,Nelson TJdoi
10.3390/ijms151120948subject
Has Abstractpub_date
2014-11-14 00:00:00pages
20948-67issue
11issn
1422-0067pii
ijms151120948journal_volume
15pub_type
杂志文章,评审abstract::The spatiotemporal regulation of calcium (Ca2+) storage in late endosomes (LE) and lysosomes (Lys) is increasingly recognized to influence a variety of membrane trafficking events, including endocytosis, exocytosis, and autophagy. Alterations in Ca2+ homeostasis within the LE/Lys compartment are implicated in human di...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19051444
更新日期:2018-05-12 00:00:00
abstract::Sertoli cells are somatic supporting cells in spermatogenic niche and play critical roles in germ cell development, but it is yet to be understood how epigenetic modifiers regulate Sertoli cell development and contribution to spermatogenesis. BRG1 (Brahma related gene 1) is a catalytic subunit of the mammalian SWI/SNF...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124358
更新日期:2020-06-19 00:00:00
abstract::Thlaspi arvense is a well-known annual farmland weed with worldwide distribution, which can be found from sea level to above 4000 m high on the Qinghai-Tibetan Plateau (QTP). In this paper, a phylogeographic history of T. arvense including 19 populations from China was inferred by using three chloroplast (cp) DNA segm...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160613339
更新日期:2015-06-11 00:00:00
abstract::The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141224087
更新日期:2013-12-11 00:00:00
abstract::Natural killer (NK) cells play a significant and vital role in the first line of defense against infection through their ability to target cells without prior sensitization. They also contribute significantly to the activation and recruitment of both innate and adaptive immune cells through the production of a range o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228864
更新日期:2020-11-23 00:00:00
abstract::Cytokines are signaling proteins that were first characterized as components of the immune response, but have been found to have pleiotropic effects in diverse aspects of body function in health and disease. They are secreted by numerous cells and are used extensively in intercellular communications to produce differe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140713931
更新日期:2013-07-04 00:00:00
abstract::Sabicea species are used in the Amazon for treatment of fever and malaria, which suggests that its chemical constituents may have some effect on pain and inflammation. Phytochemical analysis of the hexane fraction obtained from the crude ethanol extract from Sabicea grisea var. grisea Cham. & Schltdl (Rubiaceae), an e...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13021598
更新日期:2012-01-01 00:00:00
abstract::In recent years, ongoing interest in ischemic brain injury research has provided data showing that ischemic episodes are involved in the development of Alzheimer's disease-like neuropathology. Brain ischemia is the second naturally occurring neuropathology, such as Alzheimer's disease, which causes the death of neuron...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19124002
更新日期:2018-12-12 00:00:00
abstract::Major depression is one of the leading causes of disability, morbidity, and mortality worldwide. The brain⁻gut axis functions are disturbed, revealed by a dysfunction of the brain, immune system, endocrine system, and gut. Traditional depression treatments all target the brain, with different drugs and/or psychotherap...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19061592
更新日期:2018-05-29 00:00:00
abstract::The variable nature of vascular dysfunction in diabetes is not well understood. We explored the functional adaptation of different arteries in db/db mice in relation to increased severity and duration of diabetes. We compared endothelium-dependent and -independent vasodilation in the aortae, as well as the carotid and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176147
更新日期:2020-08-26 00:00:00
abstract::This study was conducted to monitor the macrophage infiltration of atopic dermatitis (AD)-like skin lesions and to evaluate the effects of anti-AD therapeutic agents in immunocompetent mice via optical reporter-gene-based molecular imaging. The enhanced firefly luciferase (effluc)-expressing macrophage cell line (Raw2...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176205
更新日期:2020-08-27 00:00:00
abstract::Many studies have addressed several plant-insect interaction topics at nutritional, molecular, physiological, and evolutionary levels. However, it is still unknown how flexible the metabolism and the nutritional content of specialist insect herbivores feeding on different closely related plants can be. We performed el...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010059
更新日期:2018-12-24 00:00:00
abstract::Different concentrations of asafoetida extract were added to the medium of Pleurotus ferulic and the effects of the extract on growth of P. ferulic mycelium and fruiting bodies was observed. As the amount of asafoetida extract additive was increased, the growth of Pleurotus mycelium was faster, the time formation of b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11010041
更新日期:2009-12-29 00:00:00
abstract::Several studies indicate that bisphenol A (BPA) and phthalates may have a role in the development of metabolic diseases using different molecular pathways, including epigenetic regulatory mechanisms. However, it is unclear whether exposure to these chemicals modifies serum levels of miRNAs associated with gestational ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20133343
更新日期:2019-07-07 00:00:00
abstract::Of the multiple sources of reactive oxygen species (ROS) in the blood vessel, NADPH oxidases are the primary source. Whereas several studies have implicated NADPH oxidases in the initiation of atherosclerosis, their roles in disease progression are incompletely understood. Our objective was to determine the potential ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140817017
更新日期:2013-08-19 00:00:00
abstract::Breast cancer is the most common malignancy in women worldwide and can be categorized into several subtypes according to histopathological parameters or genomic signatures. Such heterogeneity of breast cancer can arise from the reactivation of mammary stem cells in situ during tumorigenesis. Moreover, different breast...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20081827
更新日期:2019-04-12 00:00:00
abstract::Keloids occur after failure of the wound healing process; inflammation persists, and various treatments are ineffective. Keloid pathogenesis is still unclear. We have previously analysed the gene expression profiles in keloid tissue and found that HtrA1 was markedly up-regulated in the keloid lesions. HtrA1 is a serin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051275
更新日期:2018-04-24 00:00:00
abstract::The particle sizes of pharmaceutical substances are important for their bioavailability. Bioavailability can be improved by reducing the particle size of the drug. In this study, artemisinin was micronized by the rapid expansion of supercritical solutions (RESS). The particle size of the unprocessed white needle-like ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13045060
更新日期:2012-01-01 00:00:00
abstract::Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of Polygonum multiflorum. We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the production of phyt...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17111912
更新日期:2016-11-16 00:00:00
abstract::Carotenoid cleavage dioxygenases (CCDs) selectively catalyze carotenoids, forming smaller apocarotenoids that are essential for the synthesis of apocarotenoid flavor, aroma volatiles, and phytohormone ABA/SLs, as well as responses to abiotic stresses. Here, 19, 11, and 10 CCD genes were identified in Nicotiana tabacum...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225796
更新日期:2019-11-18 00:00:00
abstract::Glycation is a non-enzymatic post-translational modification of proteins, formed by the reaction of reducing sugars and α-dicarbonyl products of their degradation with amino and guanidino groups of proteins. Resulted early glycation products are readily involved in further transformation, yielding a heterogeneous grou...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122557
更新日期:2017-11-28 00:00:00
abstract::The use of nanotechnology could play a significant role in the agriculture sector, especially in the preparation of new-generation agronanochemicals. Currently, the economically important plant of Malaysia, the oil palm, faces the threat of a devastating disease which is particularly caused by a pathogenic fungus, Gan...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092247
更新日期:2019-05-07 00:00:00
abstract::Arrhythmias in Brugada syndrome patients originate in the right ventricular outflow tract (RVOT). Over the past few decades, the characterization of the unique anatomy and electrophysiology of the RVOT has revealed the arrhythmogenic nature of this region. However, the mechanisms that drive arrhythmias in Brugada synd...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197051
更新日期:2020-09-25 00:00:00
abstract::This paper reports the rapid melt quenching technique preparation for the new family of bismuth-lead germanate glass (BPG) systems in the form of (GeO(2))(60)-(PbO)(40-) (x)-(½Bi(2)O(3))(x) where x = 0 to 40 mol%. Their densities with respect of Bi(2)O(3) concentration were determined using Archimedes' method with ace...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13044632
更新日期:2012-01-01 00:00:00
abstract::Oral mucositis is the most frequently occurring early side effect of head-and-neck cancer radiotherapy. Systemic dermatan sulfate (DS) treatment revealed a significant radioprotective potential in a preclinical model of oral mucositis. This study was initiated to elucidate the mechanistic effects of DS in the same mod...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19061684
更新日期:2018-06-06 00:00:00
abstract::Dry fruits consist of two types, dehiscent and indehiscent, whereby the fruit is splitting open or remains closed at maturity, respectively. The seed, the dispersal unit (DU) of dehiscent fruits, is composed of three major parts, the embryo and the food reserve, encapsulated by the maternally-derived organ, the seed c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21218024
更新日期:2020-10-28 00:00:00
abstract::In a previous study on chromate toxicity, an increase in the 2Fe2S electron paramagnetic resonance (EPR) signal from mitochondria was found upon addition of chromate to human bronchial epithelial cells and bovine airway tissue ex vivo. This study was undertaken to show that a chromate-induced increase in the 2Fe2S EPR...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051143
更新日期:2019-03-06 00:00:00
abstract::The glutamatergic system is a key point in pathogenesis of schizophrenia. Sarcosine (N-methylglycine) is an exogenous amino acid that acts as a glycine transporter inhibitor. It modulates glutamatergic transmission by increasing glycine concentration around NMDA (N-methyl-d-aspartate) receptors. In patients with schiz...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,随机对照试验
doi:10.3390/ijms161024475
更新日期:2015-10-15 00:00:00
abstract::miRNAs are small noncoding RNAs capable of regulating gene expression at the post-transcriptional level. A growing body of evidence demonstrated that let-7 family of miRNAs, as one of the highly conserved miRNAs, plays an important role in cell differentiation and development, as well as tumor suppressor function depe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151019119
更新日期:2014-10-21 00:00:00
abstract::5-Fluorouracil (5-FU) is an important chemotherapeutic agent for the systemic treatment of colorectal cancer (CRC), but its effectiveness against CRC is limited by increased 5-FU resistance caused by the hypoxic tumor microenvironment. The purpose of our study was to assess the feasibility of using quinacrine (QC) to ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184366
更新日期:2019-09-05 00:00:00