Abstract：
:Spinal cord ischemia-reperfusion injury(SCII)affects nerve function through many mechanisms, which are complex and not fully understood. Recently, accumulating evidence has indicated that long noncoding RNAs (lncRNAs) play an increasingly important role in SCII. We investigated the role of lncRNA growth arrest-specific 5(Gas5) in a rat SCII model, and its effects on apoptosis and inflammation possibly by modulating MMP-7, cleaved caspase-3 and IL-1β. LncRNA Gas5 and MMP-7 were knocked down by intrathecal siRNA injection. Neurological assessment and TUNEL assay were performed. The RNA and protein expression levels of lncRNA Gas5, MMP-7, cleaved caspase-3 and IL-1β were determined by PCR and Western blotting, respectively. MMP-7 localization was visualized by double-immunofluorescence. SCII induced functional impairment in the hind limb, and the expression of lncRNA Gas5 was highest at 24 h after SCII. LncRNA Gas5 downregulation inhibited the RNA and protein expression of MMP-7, as well as the protein expression of cleaved caspase-3 and IL-1β. LncRNA Gas5 downregulation reduced the number of TUNEL-positive and MMP-7-positive double-labeled cells. Therefore, lncRNA Gas5 downregulation alleviated hind limb functional impairment and improved neuronal apoptosis after SCII. MMP-7 downregulation also inhibited apoptosis and inflammation and alleviated damage. Pretreatment with intrathecal injection of si-lncRNA Gas5 and si-MMP-7 reduced the expression levels of cleaved caspase-3 and IL-1β, protecting nerve function after SCII. These results show that lncRNA Gas5 plays an important role in SCII, perhaps by inhibiting MMP-7, cleaved caspase-3 and IL-1β. LncRNA Gas5 downregulation could be a promising therapeutic approach in the SCII treatment.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Zhang Z,Li X,Chen F,Li Z,Wang D,Ren X,Ma Hdoi
10.1016/j.brainresbull.2020.12.005subject
Has Abstractpub_date
2020-12-11 00:00:00pages
110-119eissn
0361-9230issn
1873-2747pii
S0361-9230(20)30714-0journal_volume
168pub_type
杂志文章abstract：:In people with Huntington's disease, an expanded CAG repeat sequence on the HTT gene confers a toxic gain function resulting in a progressive and fatal neurodegeneration. The Hdh((CAG)Q150) Huntington's disease mouse line is a knock-in model of the disease that carries ∼150 CAG repeats on the normal mouse Htt locus. T...
journal_title：Brain research bulletin
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journal_title：Brain research bulletin
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2008.02.028
更新日期：2008-07-01 00:00:00
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.06.010
更新日期：2016-06-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：1984-04-01 00:00:00
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更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
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journal_title：Brain research bulletin
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Brain research bulletin
pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：1992-02-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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