当前位置： SCI文献检索 > Nature Communications期刊下所有文献 > Behavior control of membrane-less protein liquid condensates with metal ion-induced phase separation.

Behavior control of membrane-less protein liquid condensates with metal ion-induced phase separation.

Abstract：

:Phase separation of specific biomolecules into liquid droplet-like condensates is a key mechanism to form membrane-less organelles, which spatio-temporally organize diverse biochemical processes in cells. To investigate the working principles of these biomolecular condensates as dynamic reaction centers, precise control of diverse condensate properties is essential. Here, we design a strategy for metal ion-induced clustering of minimal protein modules to produce liquid protein condensates, the properties of which can be widely varied by simple manipulation of the protein clustering systems. The droplet forming-minimal module contains only a single receptor protein and a binding ligand peptide with a hexahistidine tag for divalent metal ion-mediated clustering. A wide range of protein condensate properties such as droplet forming tendency, droplet morphology, inside protein diffusivity, protein recruitment, and droplet density can be varied by adjusting the nature of receptor/ligand pairs or used metal ions, metal/protein ratios, incubation time, binding motif variation on recruited proteins, and even spacing between receptor/ligand pairs and the hexahistidine tag. We also demonstrate metal-ion-induced protein phase separation in cells. The present phase separation strategy provides highly versatile protein condensates, which will greatly facilitate investigation of molecular and structural codes of droplet-forming proteins and the monitoring of biomolecular behaviors inside diverse protein condensates.

journal_name

Nat Commun

journal_title

Nature communications

authors

Hong K,Song D,Jung Y

doi

10.1038/s41467-020-19391-8

subject

Has Abstract

pub_date

2020-11-03 00:00:00

pages

5554

issue

1

issn

2041-1723

pii

10.1038/s41467-020-19391-8

journal_volume

11

pub_type

杂志文章

相关文献

Nature Communications文献大全
  • Mutualism supports biodiversity when the direct competition is weak.

    abstract：:A key question of theoretical ecology is which properties of ecosystems favour their stability and help maintaining biodiversity. This question recently reconsidered mutualistic systems, generating intense controversy about the role of mutualistic interactions and their network architecture. Here we show analytically ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms14326

    authors： Pascual-García A,Bastolla U

    更新日期：2017-02-24 00:00:00

  • Quantum nuclear dynamics in the photophysics of diamondoids.

    abstract：:The unusual electronic properties of diamondoids, the nanoscale relatives of diamond, make them attractive for applications ranging from drug delivery to field emission displays. Identifying the fundamental origin of these properties has proven highly challenging, with even the most advanced quantum many-body calculat...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms3006

    authors： Patrick CE,Giustino F

    更新日期：2013-01-01 00:00:00

  • Printable enzyme-embedded materials for methane to methanol conversion.

    abstract：:An industrial process for the selective activation of methane under mild conditions would be highly valuable for controlling emissions to the environment and for utilizing vast new sources of natural gas. The only selective catalysts for methane activation and conversion to methanol under mild conditions are methane m...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11900

    authors： Blanchette CD,Knipe JM,Stolaroff JK,DeOtte JR,Oakdale JS,Maiti A,Lenhardt JM,Sirajuddin S,Rosenzweig AC,Baker SE

    更新日期：2016-06-15 00:00:00

  • Spatial expression analyses of the putative oncogene ciRS-7 in cancer reshape the microRNA sponge theory.

    abstract：:Circular RNAs (circRNAs) have recently gained substantial attention in the cancer research field where most, including the putative oncogene ciRS-7 (CDR1as), have been proposed to function as competitive endogenous RNAs (ceRNAs) by sponging specific microRNAs. Here, we report the first spatially resolved cellular expr...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-18355-2

    authors： Kristensen LS,Ebbesen KK,Sokol M,Jakobsen T,Korsgaard U,Eriksen AC,Hansen TB,Kjems J,Hager H

    更新日期：2020-09-11 00:00:00

  • SIRT1 mediates obesity- and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression.

    abstract：:Puberty is regulated by epigenetic mechanisms and is highly sensitive to metabolic and nutritional cues. However, the epigenetic pathways mediating the effects of nutrition and obesity on pubertal timing are unknown. Here, we identify Sirtuin 1 (SIRT1), a fuel-sensing deacetylase, as a molecule that restrains female p...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-06459-9

    authors： Vazquez MJ,Toro CA,Castellano JM,Ruiz-Pino F,Roa J,Beiroa D,Heras V,Velasco I,Dieguez C,Pinilla L,Gaytan F,Nogueiras R,Bosch MA,Rønnekleiv OK,Lomniczi A,Ojeda SR,Tena-Sempere M

    更新日期：2018-10-10 00:00:00

  • Crystal structure of an amphiphilic foldamer reveals a 48-mer assembly comprising a hollow truncated octahedron.

    abstract：:Foldamers provide an attractive medium to test the mechanisms by which biological macromolecules fold into complex three-dimensional structures, and ultimately to design novel protein-like architectures with properties unprecedented in nature. Here, we describe a large cage-like structure formed from an amphiphilic ar...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms4581

    authors： Pavone V,Zhang SQ,Merlino A,Lombardi A,Wu Y,DeGrado WF

    更新日期：2014-04-07 00:00:00

  • Molecular switches from benzene derivatives adsorbed on metal surfaces.

    abstract：:Transient precursor states are often experimentally observed for molecules adsorbing on surfaces. However, such precursor states are typically rather short-lived, quickly yielding to more stable adsorption configurations. Here we employ first-principles calculations to systematically explore the interaction mechanism ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms3569

    authors： Liu W,Filimonov SN,Carrasco J,Tkatchenko A

    更新日期：2013-01-01 00:00:00

  • Ultralow-threshold multiphoton-pumped lasing from colloidal nanoplatelets in solution.

    abstract：:Although multiphoton-pumped lasing from a solution of chromophores is important in the emerging fields of nonlinear optofluidics and bio-photonics, conventionally used organic dyes are often rendered unsuitable because of relatively small multiphoton absorption cross-sections and low photostability. Here, we demonstra...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms9513

    authors： Li M,Zhi M,Zhu H,Wu WY,Xu QH,Jhon MH,Chan Y

    更新日期：2015-09-30 00:00:00

  • Quantum spin Hall phase in 2D trigonal lattice.

    abstract：:The quantum spin Hall (QSH) phase is an exotic phenomena in condensed-matter physics. Here we show that a minimal basis of three orbitals (s, px, py) is required to produce a QSH phase via nearest-neighbour hopping in a two-dimensional trigonal lattice. Tight-binding model analyses and calculations show that the QSH p...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12746

    authors： Wang ZF,Jin KH,Liu F

    更新日期：2016-09-07 00:00:00

  • Energetics of activation of GTP hydrolysis on the ribosome.

    abstract：:Several of the steps in protein synthesis on the ribosome utilize hydrolysis of guanosine triphosphate (GTP) as the driving force. This reaction is catalyzed by translation factors that become activated upon binding to the ribosome. The recently determined crystal structure of an elongation factor-Tu ternary complex b...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms2741

    authors： Wallin G,Kamerlin SC,Aqvist J

    更新日期：2013-01-01 00:00:00

  • Structure of the mouse TRPC4 ion channel.

    abstract：:Members of the transient receptor potential (TRP) ion channels conduct cations into cells. They mediate functions ranging from neuronally mediated hot and cold sensation to intracellular organellar and primary ciliary signaling. Here we report a cryo-electron microscopy (cryo-EM) structure of TRPC4 in its unliganded (...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-05247-9

    authors： Duan J,Li J,Zeng B,Chen GL,Peng X,Zhang Y,Wang J,Clapham DE,Li Z,Zhang J

    更新日期：2018-08-06 00:00:00

  • Role of syn-eruptive plagioclase disequilibrium crystallization in basaltic magma ascent dynamics.

    abstract：:Timescales of magma ascent in conduit models are typically assumed to be much longer than crystallization and gas exsolution for basaltic eruptions. However, it is now recognized that basaltic magmas may rise fast enough for disequilibrium processes to play a key role on the ascent dynamics. The quantification of the ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13402

    authors： La Spina G,Burton M,De' Michieli Vitturi M,Arzilli F

    更新日期：2016-12-12 00:00:00

  • Stagnant forearc mantle wedge inferred from mapping of shear-wave anisotropy using S-net seafloor seismometers.

    abstract：:Shear-wave anisotropy in Earth's mantle helps constrain the lattice-preferred orientation of anisotropic minerals due to viscous flow. Previous studies at the Japan Trench subduction zone using land-based seismic networks identified strong anisotropy in the mantle wedge, reflecting viscous flow induced by the subducti...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19541-y

    authors： Uchida N,Nakajima J,Wang K,Takagi R,Yoshida K,Nakayama T,Hino R,Okada T,Asano Y

    更新日期：2020-11-10 00:00:00

  • Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation.

    abstract：:Most DNA in the genomes of higher organisms does not encode proteins, yet much is transcribed by RNA polymerase II (RNAPII) into long non-coding RNAs (lncRNAs). The biological significance of most lncRNAs is largely unclear. Here, we identify a lncRNA (SVALKA) in a cold-sensitive region of the Arabidopsis genome. Muta...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07010-6

    authors： Kindgren P,Ard R,Ivanov M,Marquardt S

    更新日期：2018-11-01 00:00:00

  • MIS-11 duration key to disappearance of the Greenland ice sheet.

    abstract：:Palaeo data suggest that Greenland must have been largely ice free during Marine Isotope Stage 11 (MIS-11). However, regional summer insolation anomalies were modest during this time compared to MIS-5e, when the Greenland ice sheet likely lost less volume. Thus it remains unclear how such conditions led to an almost c...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms16008

    authors： Robinson A,Alvarez-Solas J,Calov R,Ganopolski A,Montoya M

    更新日期：2017-07-06 00:00:00

  • Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification.

    abstract：:Chromatin looping allows enhancer-bound regulatory factors to influence transcription. Large domains, referred to as topologically associated domains, participate in genome organization. However, the mechanisms underlining interactions within these domains, which control gene expression, are not fully understood. Here...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-10318-6

    authors： Magli A,Baik J,Pota P,Cordero CO,Kwak IY,Garry DJ,Love PE,Dynlacht BD,Perlingeiro RCR

    更新日期：2019-05-24 00:00:00

  • PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation.

    abstract：:Despite the global impact of macrophage activation in vascular disease, the underlying mechanisms remain obscure. Here we show, with global proteomic analysis of macrophage cell lines treated with either IFNγ or IL-4, that PARP9 and PARP14 regulate macrophage activation. In primary macrophages, PARP9 and PARP14 have o...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12849

    authors： Iwata H,Goettsch C,Sharma A,Ricchiuto P,Goh WW,Halu A,Yamada I,Yoshida H,Hara T,Wei M,Inoue N,Fukuda D,Mojcher A,Mattson PC,Barabási AL,Boothby M,Aikawa E,Singh SA,Aikawa M

    更新日期：2016-10-31 00:00:00

  • Novel pleiotropic risk loci for melanoma and nevus density implicate multiple biological pathways.

    abstract：:The total number of acquired melanocytic nevi on the skin is strongly correlated with melanoma risk. Here we report a meta-analysis of 11 nevus GWAS from Australia, Netherlands, UK, and USA comprising 52,506 individuals. We confirm known loci including MTAP, PLA2G6, and IRF4, and detect novel SNPs in KITLG and a regio...

    journal_title：Nature communications

    pub_type： 杂志文章,meta分析

    doi：10.1038/s41467-018-06649-5

    authors： Duffy DL,Zhu G,Li X,Sanna M,Iles MM,Jacobs LC,Evans DM,Yazar S,Beesley J,Law MH,Kraft P,Visconti A,Taylor JC,Liu F,Wright MJ,Henders AK,Bowdler L,Glass D,Ikram MA,Uitterlinden AG,Madden PA,Heath AC,Nelson EC,

    更新日期：2018-11-14 00:00:00

  • Repurposing DNA-binding agents as H-bonded organic semiconductors.

    abstract：:Organic semiconductors are usually polycyclic aromatic hydrocarbons and their analogs containing heteroatom substitution. Bioinspired materials chemistry of organic electronics promises new charge transport mechanism and specific molecular recognition with biomolecules. We discover organic semiconductors from deoxyrib...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-12248-9

    authors： Zhang F,Lemaur V,Choi W,Kafle P,Seki S,Cornil J,Beljonne D,Diao Y

    更新日期：2019-09-16 00:00:00

  • Structural basis of subtype-selective competitive antagonism for GluN2C/2D-containing NMDA receptors.

    abstract：:N-Methyl-D-aspartate receptors (NMDARs) play critical roles in the central nervous system. Their heterotetrameric composition generates subtypes with distinct functional properties and spatio-temporal distribution in the brain, raising the possibility for subtype-specific targeting by pharmacological means for treatme...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-14321-0

    authors： Wang JX,Irvine MW,Burnell ES,Sapkota K,Thatcher RJ,Li M,Simorowski N,Volianskis A,Collingridge GL,Monaghan DT,Jane DE,Furukawa H

    更新日期：2020-01-22 00:00:00

  • Targeted sequencing reveals clonal genetic changes in the progression of early lung neoplasms and paired circulating DNA.

    abstract：:Lungs resected for adenocarcinomas often harbour minute discrete foci of cytologically atypical pneumocyte proliferations designated as atypical adenomatous hyperplasia (AAH). Evidence suggests that AAH represents an initial step in the progression to adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MI...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms9258

    authors： Izumchenko E,Chang X,Brait M,Fertig E,Kagohara LT,Bedi A,Marchionni L,Agrawal N,Ravi R,Jones S,Hoque MO,Westra WH,Sidransky D

    更新日期：2015-09-16 00:00:00

  • BRCA2 abrogation triggers innate immune responses potentiated by treatment with PARP inhibitors.

    abstract：:Heterozygous germline mutations in BRCA2 predispose to breast and ovarian cancer. Contrary to non-cancerous cells, where BRCA2 deletion causes cell cycle arrest or cell death, tumors carrying BRCA2 inactivation continue to proliferate. Here we set out to investigate adaptation to loss of BRCA2 focusing on genome-wide ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-11048-5

    authors： Reisländer T,Lombardi EP,Groelly FJ,Miar A,Porru M,Di Vito S,Wright B,Lockstone H,Biroccio A,Harris A,Londoño-Vallejo A,Tarsounas M

    更新日期：2019-07-17 00:00:00

  • Mechano-responsive hydrogen-bonding array of thermoplastic polyurethane elastomer captures both strength and self-healing.

    abstract：:Self-repairable materials strive to emulate curable and resilient biological tissue; however, their performance is currently insufficient for commercialization purposes because mending and toughening are mutually exclusive. Herein, we report a carbonate-type thermoplastic polyurethane elastomer that self-heals at 35 °...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-021-20931-z

    authors： Eom Y,Kim SM,Lee M,Jeon H,Park J,Lee ES,Hwang SY,Park J,Oh DX

    更新日期：2021-01-27 00:00:00

  • Fine-mapping of prostate cancer susceptibility loci in a large meta-analysis identifies candidate causal variants.

    abstract：:Prostate cancer is a polygenic disease with a large heritable component. A number of common, low-penetrance prostate cancer risk loci have been identified through GWAS. Here we apply the Bayesian multivariate variable selection algorithm JAM to fine-map 84 prostate cancer susceptibility loci, using summary data from a...

    journal_title：Nature communications

    pub_type： 杂志文章,meta分析

    doi：10.1038/s41467-018-04109-8

    authors： Dadaev T,Saunders EJ,Newcombe PJ,Anokian E,Leongamornlert DA,Brook MN,Cieza-Borrella C,Mijuskovic M,Wakerell S,Olama AAA,Schumacher FR,Berndt SI,Benlloch S,Ahmed M,Goh C,Sheng X,Zhang Z,Muir K,Govindasami K,Lophatan

    更新日期：2018-06-11 00:00:00

  • Copolymer dielectrics with balanced chain-packing density and surface polarity for high-performance flexible organic electronics.

    abstract：:The ever-increasing demand for flexible electronics calls for the development of low-voltage and high-mobility organic thin-film transistors (OTFTs) that can be integrated into emerging display and labeling technologies. Polymer dielectrics with comprehensive and balanced dielectric properties (i.e., a good balance be...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04665-z

    authors： Ji D,Li T,Zou Y,Chu M,Zhou K,Liu J,Tian G,Zhang Z,Zhang X,Li L,Wu D,Dong H,Miao Q,Fuchs H,Hu W

    更新日期：2018-06-14 00:00:00

  • Long-range transport of airborne microbes over the global tropical and subtropical ocean.

    abstract：:The atmosphere plays a fundamental role in the transport of microbes across the planet but it is often neglected as a microbial habitat. Although the ocean represents two thirds of the Earth's surface, there is little information on the atmospheric microbial load over the open ocean. Here we provide a global estimate ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-00110-9

    authors： Mayol E,Arrieta JM,Jiménez MA,Martínez-Asensio A,Garcias-Bonet N,Dachs J,González-Gaya B,Royer SJ,Benítez-Barrios VM,Fraile-Nuez E,Duarte CM

    更新日期：2017-08-04 00:00:00

  • A strategy to discover new organizers identifies a putative heart organizer.

    abstract：:Organizers are regions of the embryo that can both induce new fates and impart pattern on other regions. So far, surprisingly few organizers have been discovered, considering the number of patterned tissue types generated during development. This may be because their discovery has relied on transplantation and ablatio...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12656

    authors： Anderson C,Khan MAF,Wong F,Solovieva T,Oliveira NMM,Baldock RA,Tickle C,Burt DW,Stern CD

    更新日期：2016-08-25 00:00:00

  • A clinically applicable deep-learning model for detecting intracranial aneurysm in computed tomography angiography images.

    abstract：:Intracranial aneurysm is a common life-threatening disease. Computed tomography angiography is recommended as the standard diagnosis tool; yet, interpretation can be time-consuming and challenging. We present a specific deep-learning-based model trained on 1,177 digital subtraction angiography verified bone-removal co...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19527-w

    authors： Shi Z,Miao C,Schoepf UJ,Savage RH,Dargis DM,Pan C,Chai X,Li XL,Xia S,Zhang X,Gu Y,Zhang Y,Hu B,Xu W,Zhou C,Luo S,Wang H,Mao L,Liang K,Wen L,Zhou L,Yu Y,Lu GM,Zhang LJ

    更新日期：2020-11-30 00:00:00

  • H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis.

    abstract：:Dynamic changes in histone modifications under various physiological cues play important roles in gene transcription and cancer. Identification of new histone marks critical for cancer development is of particular importance. Here we show that, in a glucose-dependent manner, E3 ubiquitin ligase NEDD4 ubiquitinates his...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms14799

    authors： Zhang X,Li B,Rezaeian AH,Xu X,Chou PC,Jin G,Han F,Pan BS,Wang CY,Long J,Zhang A,Huang CY,Tsai FJ,Tsai CH,Logothetis C,Lin HK

    更新日期：2017-03-16 00:00:00

  • Single spin localization and manipulation in graphene open-shell nanostructures.

    abstract：:Turning graphene magnetic is a promising challenge to make it an active material for spintronics. Predictions state that graphene structures with specific shapes can spontaneously develop magnetism driven by Coulomb repulsion of π-electrons, but its experimental verification is demanding. Here, we report on the observ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-08060-6

    authors： Li J,Sanz S,Corso M,Choi DJ,Peña D,Frederiksen T,Pascual JI

    更新日期：2019-01-14 00:00:00