Abstract:
:STIM1 is an endoplasmic reticulum (ER) protein that modulates the activity of a number of Ca2+ transport systems. By direct physical interaction with ORAI1, a plasma membrane Ca2+ channel, STIM1 activates the ICRAC current, whereas the binding with the voltage-operated Ca2+ channel CaV1.2 inhibits the current through this latter channel. In this way, STIM1 is a key regulator of Ca2+ signaling in excitable and non-excitable cells, and altered STIM1 levels have been reported to underlie several pathologies, including immunodeficiency, neurodegenerative diseases, and cancer. In both sporadic and familial Alzheimer's disease, a decrease of STIM1 protein levels accounts for the alteration of Ca2+ handling that compromises neuronal cell viability. Using SH-SY5Y cells edited by CRISPR/Cas9 to knockout STIM1 gene expression, this work evaluated the molecular mechanisms underlying the cell death triggered by the deficiency of STIM1, demonstrating that STIM1 is a positive regulator of ITPR3 gene expression. ITPR3 (or IP3R3) is a Ca2+ channel enriched at ER-mitochondria contact sites where it provides Ca2+ for transport into the mitochondria. Thus, STIM1 deficiency leads to a strong reduction of ITPR3 transcript and ITPR3 protein levels, a consequent decrease of the mitochondria free Ca2+ concentration ([Ca2+]mit), reduction of mitochondrial oxygen consumption rate, and decrease in ATP synthesis rate. All these values were normalized by ectopic expression of ITPR3 in STIM1-KO cells, providing strong evidence for a new mode of regulation of [Ca2+]mit mediated by the STIM1-ITPR3 axis.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Pascual-Caro C,Orantos-Aguilera Y,Sanchez-Lopez I,de Juan-Sanz J,Parys JB,Area-Gomez E,Pozo-Guisado E,Martin-Romero FJdoi
10.3390/ijms21186598subject
Has Abstractpub_date
2020-09-09 00:00:00issue
18issn
1422-0067pii
ijms21186598journal_volume
21pub_type
杂志文章abstract::Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult car...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160714511
更新日期:2015-06-26 00:00:00
abstract::Modern antipsychotic drugs are employed increasingly in the treatment of mood disorders as well as psychoses, stimulating interest in their possible contributions to altering suicidal risk. Clozapine remains the only treatment with an FDA-recognized indication for reducing suicidal risk (in schizophrenia). We carried ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17101700
更新日期:2016-10-11 00:00:00
abstract::Circulating microRNAs (miRNAs) hold great potential as novel diagnostic markers for acute coronary syndrome (ACS). This study sought to identify plasma miRNAs that are differentially expressed in young ACS patients (mean age of 38.5 ± 4.3 years) and evaluate their diagnostic potentials. Small RNA sequencing (sRNA-seq)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051467
更新日期:2018-05-15 00:00:00
abstract::The ongoing pandemic of coronavirus disease-2019 (COVID-19) is being caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The disease continues to present significant challenges to the health care systems around the world. This is primarily because of the lack of vaccines to protect against the infe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21155224
更新日期:2020-07-23 00:00:00
abstract::The microRNAs are small RNAs that regulate gene expression at the post-transcriptional level and can be involved in the onset of neurodegenerative diseases and cancer. They are emerging as possible targets for antisense-based therapy, even though the in vivo stability of miRNA analogues is still questioned. We tested ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062001
更新日期:2020-03-15 00:00:00
abstract::Spontaneous beating of the heart pacemaker, the sinoatrial node, is generated by sinoatrial node cells (SANC) and caused by gradual change of the membrane potential called diastolic depolarization (DD). Submembrane local Ca2+ releases (LCR) from sarcoplasmic reticulum (SR) occur during late DD and activate an inward N...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19082173
更新日期:2018-07-25 00:00:00
abstract::To evaluate whether chronic cocaine abuse will increase cardiac Fas-dependent and mitochondria-dependent apoptotic pathways, thirty-two male Wistar rats at 3-4 months of age were randomly divided into a vehicle-treated group (phosphate-buffered saline, PBS, 0.5 mL, SQ per day) and a cocaine-treated group (Cocaine, 10 ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15045988
更新日期:2014-04-09 00:00:00
abstract:BACKGROUND:CD73 is an ectonucleotidase regulating extracellular adenosine concentration and plays an important role in adenosine-mediated immunosuppressive pathways. The efficacy of CD73-targeted therapy depends on the expression levels of CD73; therefore, monitoring CD73 status in cancer patients would provide helpful...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072304
更新日期:2020-03-26 00:00:00
abstract::Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112458
更新日期:2017-11-18 00:00:00
abstract::Human Ribonuclease 6 is a secreted protein belonging to the ribonuclease A (RNaseA) superfamily, a vertebrate specific family suggested to arise with an ancestral host defense role. Tissue distribution analysis revealed its expression in innate cell types, showing abundance in monocytes and neutrophils. Recent evidenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17040552
更新日期:2016-04-13 00:00:00
abstract::Neonatal hypoxia-ischemia (HI) is a major cause of death and disability in neonates. HI leads to a dramatic rise in intracellular calcium levels, which was originally thought to be detrimental to the brain. However, it has been increasingly recognized that this calcium signaling may also play an important protective r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092063
更新日期:2019-04-26 00:00:00
abstract::Prostate cancer (PC) is a frequent male malignancy and represents the second most diagnosed cancer in men. Since pre-cancerous lesions, i.e., the high-grade prostatic intraepithelial neoplasia (HGPIN), can be detected years before progression to PC, early diagnosis and chemoprevention are targeted strategies to reduce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15011433
更新日期:2014-01-21 00:00:00
abstract::DNA damage-regulated autophagy modulator protein 1 (DRAM1), a multi-pass membrane lysosomal protein, is reportedly a tumor protein p53 (TP53) target gene involved in autophagy. During cerebral ischemia/reperfusion (I/R) injury, DRAM1 protein expression is increased, and autophagy is activated. However, the functional ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151019253
更新日期:2014-10-23 00:00:00
abstract::Polymer-based piezoelectric biomaterials have already proven their relevance for tissue engineering applications. Furthermore, the morphology of the scaffolds plays also an important role in cell proliferation and differentiation. The present work reports on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), a biocompat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082149
更新日期:2018-07-24 00:00:00
abstract::Genome instability is an essential hallmark in tumor development, including colorectal cancer. We have recently identified the oxysterol binding protein-related protein 3 (ORP3), also known as oxysterol binding protein-like 3 (OSBPL3), as a novel ploidy-control gene, whose knock-out leads to aneuploidy induction and p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165894
更新日期:2020-08-16 00:00:00
abstract::Virus-like nanoparticles (VLNPs) have been studied extensively as nanocarriers for targeted drug delivery to cancer cells. However, VLNPs have intrinsic drawbacks, in particular, potential antigenicity and immunogenicity, which hamper their clinical applications. Thus, they can be eliminated easily and rapidly by host...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194903
更新日期:2019-10-03 00:00:00
abstract::RNA editing aims to treat genetic disease through altering gene expression at the transcript level. Pairing site-directed RNA-targeting mechanisms with engineered deaminase enzymes allows for the programmable correction of G>A and T>C mutations in RNA. This offers a promising therapeutic approach for a range of geneti...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030777
更新日期:2020-01-25 00:00:00
abstract::The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active prot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051635
更新日期:2020-02-27 00:00:00
abstract::Amino acid transporters alanine-serine-cysteine transporter 2 (ASCT2) and L-Type Amino Acid Transporter 1 (LAT1) are coordinately enhanced in human cancers where among other roles, they are thought to drive mechanistic target-of-rapamycin (mTOR) growth signaling. To assess ASCT2 and LAT1 as therapeutic targets, nine u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072093
更新日期:2018-07-19 00:00:00
abstract::Citral is well known for its antimicrobial, antifungal, and insecticidal activities. Natural sesquiterpene α-methylenelactones also exhibit a broad spectrum of biological activities. The aim of the study was to explore the effect of structural changes to citral molecules on citral behavior-modifying activity towards M...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218029
更新日期:2020-10-28 00:00:00
abstract::Advances in the use of targeted and immune therapies have revolutionized the clinical management of melanoma patients, prolonging significantly their overall and progression-free survival. However, both targeted and immune therapies suffer limitations due to genetic mutations and epigenetic modifications, which determ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124544
更新日期:2020-06-26 00:00:00
abstract::Of the multiple sources of reactive oxygen species (ROS) in the blood vessel, NADPH oxidases are the primary source. Whereas several studies have implicated NADPH oxidases in the initiation of atherosclerosis, their roles in disease progression are incompletely understood. Our objective was to determine the potential ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140817017
更新日期:2013-08-19 00:00:00
abstract::The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cyto...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197038
更新日期:2020-09-24 00:00:00
abstract::Nonylphenol polyethoxylates (NPnEOs), a major class of nonionic surfactants, can easily enter into aquatic environments through various pathways due to their wide applications, which leads to the extensive existence of their relative stable metabolites, namely nonylphenol (NP) and mono- to tri-ethoxylates. This study ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15011255
更新日期:2014-01-17 00:00:00
abstract::The pancreas is a key organ involved in digestion and endocrine functions in the body. The major diseases of the pancreas include pancreatitis, pancreatic cancer, cystic diseases, pancreatic divisum, islet cell tumors, endocrine tumors, diabetes mellitus, and pancreatic pain induced by these diseases. While various th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19113415
更新日期:2018-10-31 00:00:00
abstract::Dietary fats and sugars were identified as risk factors for overweight and neurodegeneration, especially in middle-age, an earlier stage of the aging process. Therefore, our aim was to study the metabolic response of both white adipose tissue and brain in middle aged rats fed a typical Western diet (high in saturated ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030786
更新日期:2020-01-25 00:00:00
abstract::Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11052200
更新日期:2010-05-20 00:00:00
abstract::The lipophilic composition of wild Arbutus unedo L. berries, collected from six locations in Penacova (center of Portugal), as well as some general chemical parameters, namely total soluble solids, pH, titratable acidity, total phenolic content and antioxidant activity was studied in detail to better understand its po...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160614194
更新日期:2015-06-23 00:00:00
abstract::Therapeutic efficacy of cisplatin-based chemotherapy for advanced-stage urothelial carcinoma (UC) is limited by drug resistance. The nuclear factor (erythroid-derived 2)-like 2 (NRF2) pathway is a major regulator of cytoprotective responses. We investigated its involvement in cisplatin resistance in long-term cisplati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081680
更新日期:2017-08-02 00:00:00
abstract::Glutamate is not only a neurotransmitter but also an important neurotoxin in central nervous system (CNS). Chronic elevation of glutamate induces both neuronal and glial cell apoptosis. However, its effect on astrocytes is complex and still remains unclear. In this study, we investigated whether morphine, a common opi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101523
更新日期:2016-10-24 00:00:00