Abstract：
:Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 mug/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 mug/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Aramwit P,Kanokpanont S,Nakpheng T,Srichana Tdoi
10.3390/ijms11052200subject
Has Abstractpub_date
2010-05-20 00:00:00pages
2200-11issue
5issn
1422-0067journal_volume
11pub_type
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