Abstract:
:The proliferative response of human white cells to stimuli such as foreign histocompatibility antigens or mitogens is traditionally assessed by measuring the amount of [3H]thymidine which the cells can incorporate in culture. In the present paper a new approach is described in which bromodeoxyuridine (BrdUrd) is used in place of thymidine and measurement of incorporation of this molecule is performed by an immunoenzymatic assay (using monoclonal anti-BrdUrd). The procedure avoids the expense, time and hazards associated with scintillation counting, and is simpler to perform. It also appears to be particularly sensitive to low levels of proliferation, and may thus detect such responses more effectively than thymidine incorporation.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Magaud JP,Sargent I,Mason DYdoi
10.1016/0022-1759(88)90276-1subject
Has Abstractpub_date
1988-01-21 00:00:00pages
95-100issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(88)90276-1journal_volume
106pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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