Selective translation by alternative bacterial ribosomes.

abstract：:A double-antibody radioimmunoassay has been developed for the detection of Australia antigen and antibodies to Australia antigen in human sera. The assay is simple to perform, and can be completed in 18 hr. Based on parallel studies of human sera, it is approximately 2000 times more sensitive than agar gel diffusion a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aach RD,Grisham JW,Parker CW

更新日期：1971-05-01 00:00:00

abstract：:Molecular oxygen (O(2)) began to accumulate in the atmosphere and surface ocean ca. 2,400 million years ago (Ma), but the persistent oxygenation of water masses throughout the oceans developed much later, perhaps beginning as recently as 580-550 Ma. For much of the intervening interval, moderately oxic surface waters ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnston DT,Wolfe-Simon F,Pearson A,Knoll AH

更新日期：2009-10-06 00:00:00

abstract：:Growth inhibition assays indicated that the IC50 values for methotrexate (MTX) and 5-fluorodeoxyuridine (FdUrd) in HS-18, a liposarcoma cell line lacking retinoblastoma protein (pRB), and SaOS-2, an osteosarcoma cell line with a truncated and nonfunctional pRB, were 10- to 12-fold and 4- to 11-fold higher, respectivel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li W,Fan J,Hochhauser D,Banerjee D,Zielinski Z,Almasan A,Yin Y,Kelly R,Wahl GM,Bertino JR

更新日期：1995-10-24 00:00:00

abstract：:The LSD1-CoREST histone demethylase complex is required to repress neuronal genes in nonneuronal tissues. Here we show that sumoylation of Braf35, one of the subunits of the complex, is required to maintain full repression of neuron-specific genes and for occupancy of the LSD1-CoREST complex at its gene targets. Inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ceballos-Chávez M,Rivero S,García-Gutiérrez P,Rodríguez-Paredes M,García-Domínguez M,Bhattacharya S,Reyes JC

更新日期：2012-05-22 00:00:00

abstract：:Vertebrate primary cilium is a Hedgehog signaling center but the extent of its involvement in other signaling systems is less well understood. This report delineates a mechanism by which fibroblast growth factor (FGF) controls primary cilia. Employing proteomic approaches to characterize proteins associated with the F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kunova Bosakova M,Nita A,Gregor T,Varecha M,Gudernova I,Fafilek B,Barta T,Basheer N,Abraham SP,Balek L,Tomanova M,Fialova Kucerova J,Bosak J,Potesil D,Zieba J,Song J,Konik P,Park S,Duran I,Zdrahal Z,Smajs D,Jans

更新日期：2019-03-05 00:00:00

abstract：:Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) belong to the Cucumovirus genus. They have a tripartite genome consisting of single-stranded RNAs, designated 1, 2, and 3. Previous studies have shown that viable pseudorecombinants could be created in vitro by reciprocal exchanges between CMV and TAV RNA 3, b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuta C,Ueda S,Suzuki M,Uyeda I

更新日期：1998-09-01 00:00:00

abstract：:Monospecific antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a BALB/c mouse immunized with rat skeletal myofibrils. After cloning 3 times on agarose, two stable clones were obtained and chosen for further characterization. The first clone, JLB1, produced an antibody that recogniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin JJ

更新日期：1981-04-01 00:00:00

abstract：:T cells react to extremely small numbers of activating agonist peptides. Spatial organization of T-cell receptors (TCR) and their peptide-major histocompatibility complex (pMHC) ligands into microclusters is correlated with T-cell activation. Here we have designed an experimental strategy that enables control over the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manz BN,Jackson BL,Petit RS,Dustin ML,Groves J

更新日期：2011-05-31 00:00:00

abstract：:Cognitive studies of memory processes demonstrate that memory for stimuli is a function of how they are encoded; stimuli processed semantically are better remembered than those processed in a perceptual or shallow fashion. This study investigates the neural correlates of this cognitive phenomenon. Twelve subjects perf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kapur S,Craik FI,Tulving E,Wilson AA,Houle S,Brown GM

更新日期：1994-03-15 00:00:00

abstract：:Pristine meteoritic materials carry light element isotopic fractionations that constrain physiochemical conditions during solar system formation. Here we report the discovery of a unique xenolith in the metal-rich chondrite Isheyevo. Its fine-grained, highly pristine mineralogy has similarity with interplanetary dust ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Briani G,Gounelle M,Marrocchi Y,Mostefaoui S,Leroux H,Quirico E,Meibom A

更新日期：2009-06-30 00:00:00

abstract：:The emergence of bacterial multidrug resistance to antibiotics threatens to cause regression to the preantibiotic era. Here we present the crystal structure of the large ribosomal subunit from Staphylococcus aureus, a versatile Gram-positive aggressive pathogen, and its complexes with the known antibiotics linezolid a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyal Z,Matzov D,Krupkin M,Wekselman I,Paukner S,Zimmerman E,Rozenberg H,Bashan A,Yonath A

更新日期：2015-10-27 00:00:00

abstract：:Genomes exhibit diverse patterns of species-specific GC content, GC and AT skews, codon bias, and mutation bias. Despite intensive investigations and the rapid accumulation of sequence data, the causes of these a priori different genome biases have not been agreed on and seem multifactorial and idiosyncratic. We show ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vetsigian K,Goldenfeld N

更新日期：2009-01-06 00:00:00

abstract：:The rise of peptides with secondary structures and functions would have been a key step in the chemical evolution which led to life. As with modern biology, amino acid sequence would have been a primary determinant of peptide structure and activity in an origins-of-life scenario. It is a commonly held hypothesis that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Forsythe JG,Petrov AS,Millar WC,Yu SS,Krishnamurthy R,Grover MA,Hud NV,Fernández FM

更新日期：2017-09-12 00:00:00

abstract：:Computers are organized into hardware and software. Using a theoretical approach to identify patterns in gene expression in a variety of species, organs, and cell types, we found that biological systems similarly are comprised of a relatively unchanging hardware-like gene pattern. Orthogonal patterns of software-like ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kravchenko-Balasha N,Levitzki A,Goldstein A,Rotter V,Gross A,Remacle F,Levine RD

更新日期：2012-03-20 00:00:00

abstract：:DNA synthesized in vitro from a phage M13 template has been cleaved at either guanine, adenine, or cytosine residues by ribosubstitution techniques. Fingerprints of the fragments obtained suggest that DNA sequencing will be possible with this technique. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salser W,Fry K,Brunk C,Poon R

更新日期：1972-01-01 00:00:00

abstract：:Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival. We have used NMR to investigate the interaction of Pin1 with three related ligands that include a pS-P substrate peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Namanja AT,Wang XJ,Xu B,Mercedes-Camacho AY,Wilson KA,Etzkorn FA,Peng JW

更新日期：2011-07-26 00:00:00

abstract：:The SCF ubiquitin ligase complex of budding yeast triggers DNA replication by catalyzing ubiquitination of the S phase cyclin-dependent kinase inhibitor SIC1. SCF is composed of three proteins-ySKP1, CDC53 (Cullin), and the F-box protein CDC4-that are conserved from yeast to humans. As part of an effort to identify co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyapina SA,Correll CC,Kipreos ET,Deshaies RJ

更新日期：1998-06-23 00:00:00

abstract：:The microbiota is now recognized as a key influence on the host immune response in the central nervous system (CNS). As such, there has been some progress toward therapies that modulate the microbiota with the aim of limiting immune-mediated demyelination, as occurs in multiple sclerosis. However, remyelination-the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMurran CE,Guzman de la Fuente A,Penalva R,Ben Menachem-Zidon O,Dombrowski Y,Falconer J,Gonzalez GA,Zhao C,Krause FN,Young AMH,Griffin JL,Jones CA,Hollins C,Heimesaat MM,Fitzgerald DC,Franklin RJM

更新日期：2019-12-10 00:00:00

abstract：:The number of x-irradiated mammalian cells surviving is markedly increased when the cells are incubated with an uncoupler of oxidative phosphorylation prior to or immediately after irradiation. This increase is greater in plateau-phase cells than in exponentially growing cells. The increase in survival is related to t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laval F

更新日期：1980-05-01 00:00:00

abstract：:Experiments were designed with the aim of understanding the mechanism whereby the lambda repressor inhibits messenger RNA synthesis. Complexes containing lambda DNA and RNA polymerase were isolated from repressed and derepressed lysogens and analyzed for the relative amounts of RNA polymerase per phage genome. The dat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayward WS,Green MH

更新日期：1969-11-01 00:00:00

abstract：:A major challenge facing the genetics of autism spectrum disorders (ASDs) is the large and growing number of candidate risk genes and gene variants of unknown functional significance. Here, we used Caenorhabditis elegans to systematically functionally characterize ASD-associated genes in vivo. Using our custom machine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McDiarmid TA,Belmadani M,Liang J,Meili F,Mathews EA,Mullen GP,Hendi A,Wong WR,Rand JB,Mizumoto K,Haas K,Pavlidis P,Rankin CH

更新日期：2020-01-07 00:00:00

abstract：:Cell adhesion molecules (CAMs) may act as regulators of morphogenesis by constraining cell motion, forming borders, and controlling intercellular communications that lead to embryonic induction. This postulated causal role of CAMs in inductive events was tested here in an in vitro system of feather induction. In the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallin WJ,Chuong CM,Finkel LH,Edelman GM

更新日期：1986-11-01 00:00:00

abstract：:The advent of molecular tension probes for real-time mapping of piconewton forces in living systems has had a major impact on mechanobiology. For example, DNA-based tension probes have revealed roles for mechanics in platelet, B cell, T cell, and fibroblast function. Nonetheless, imaging short-lived forces transmitted...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma R,Kellner AV,Ma VP,Su H,Deal BR,Brockman JM,Salaita K

更新日期：2019-08-20 00:00:00

abstract：:Cells from the spleens and thymuses of BALB/c mice whose Moloney sarcoma virus (MSV)-induced, primary sarcomas have regressed 2-3 months earlier ("MSV regressors") or are in the process of regressing can, when adoptively transferred to syngeneic mice given MSV at the age of 20 days, prevent the natural regression of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hellström I,Hellström KE,Bernstein ID

更新日期：1979-10-01 00:00:00

abstract：:The relationship between (187)Os/(186)Os and (143)Nd/(144)Nd in different manganese nodule fields is used to determine the (187)Os/(186)Os ratio of the continental terrains bounding the major ocean basins. The Atlantic Ocean drainages yield (187)Os/(186)Os of about 11; the Pacific Ocean, between 25 and 36; and the wes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turekian KK,Luck JM

更新日期：1984-12-01 00:00:00

abstract：:The nucleotide sequences of eight origin-defective mutants of simian virus 40 have been determined. All of the mutants have suffered deletions, which range in size from 4 to 241 nucleotides. Some of the mutants induce the synthesis of tumor (T) antigen, others do not. Viral mRNA extracted from rat cells transformed by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluzman Y,Sambrook JF,Frisque RJ

更新日期：1980-07-01 00:00:00

abstract：:The blanket resistance of methicillin-resistant Staphylococcus aureus to all beta-lactam antibiotics--which had such a devastating impact on chemotherapy of staphylococcal infections--is related to the properties of the key component of this resistance mechanism: the "acquired" penicillin-binding protein (PBP)-2A, whi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pinho MG,de Lencastre H,Tomasz A

更新日期：2001-09-11 00:00:00

abstract：:We have shown that a scanning transmission electron microscope with a high brightness field emission source is capable of obtaining better than 3 A resolution using 30 to 40 keV electrons. Elastic dark field images of single atoms of uranium and mercury are shown which demonstrate this fact as determined by a modified...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wall J,Langmore J,Isaacson M,Crewe AV

更新日期：1974-01-01 00:00:00

abstract：:The functional capacities of CD8(+) T cells important for virus clearance are influenced by interactions with antigen presenting cells (APCs) and CD4(+) T cells during initial selection, subsequent expansion, and development of memory. Recently, investigators have shown that polyfunctional T cells correlate best with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ndhlovu ZM,Oelke M,Schneck JP,Griffin DE

更新日期：2010-02-23 00:00:00

abstract：:The bacterial pathogen Vibrio parahaemolyticus utilizes a type III secretion system to cause death of host cells within hours of infection. We report that cell death is completely independent of apoptosis and occurs by a mechanism in which injection of multiple type III effectors causes induction of autophagy, cell ro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burdette DL,Yarbrough ML,Orvedahl A,Gilpin CJ,Orth K

更新日期：2008-08-26 00:00:00