Abstract：
:Non-ionic, low-osmolar contrast agents (CAs) used for computed tomography, such as Optiray (ioversol) and Iomeron (iomeprol), are associated with the reduced risk of adverse reactions and toxicity in comparison with ionic CAs, such as Hexabrix. Hexabrix has previously been used for imaging articular cartilage but has been commercially discontinued. This study aimed to evaluate the efficacy of Optiray and Iomeron as alternatives for visualisation of articular cartilage in small animal joints using contrast-enhanced micro-computed tomography (CECT). For this purpose, mouse femora were immersed in different concentrations (20%-50%) of Optiray 350 or Iomeron 350 for periods of time starting at five minutes. The femoral condyles were scanned ex vivo using CECT, and regions of articular cartilage manually contoured to calculate mean attenuation at each time point and concentration. For both CAs, a 30% CA concentration produced a mean cartilage attenuation optimally distinct from both bone and background signal, whilst 5-min immersion times were sufficient for equilibration of CA absorption. Additionally, plugs of bovine articular cartilage were digested by chondroitinase ABC to produce a spectrum of glycosaminoglycan (GAG) content. These samples were immersed in CA and assessed for any correlation between mean attenuation and GAG content. No significant correlation was found between attenuation and cartilage GAG content for either CAs. In conclusion, Optiray and Iomeron enable high-resolution morphological assessment of articular cartilage in small animals using CECT; however, they are not indicative of GAG content.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Ter Voert CEM,Kour RYN,van Teeffelen BCJ,Ansari N,Stok KSdoi
10.1111/joa.13271subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1062-1071issue
6eissn
0021-8782issn
1469-7580journal_volume
237pub_type
杂志文章abstract：:Retrograde fluorescent transport of Fast Blue (FB) and Diamidino Yellow (DY) was used to study the localisation of neurons that innervate the palpebral conjunctiva and the superior tarsal muscle in the cynomolgous monkey. Labelled cell bodies of sensory neurons including a few double labelled cell bodies were found in...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:During ageing, a progressive loss of skeletal muscle mass and a decrease in muscle strength and endurance take place, in the condition termed sarcopenia. The mechanisms of sarcopenia are complex and still unclear; however, it is known that muscle atrophy is associated with a decline in the number and/or efficiency of ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12429
更新日期：2016-05-01 00:00:00
abstract：:The ultrastructure of the basal lamina of histologically normal human breast tissue was determined in 19 women undergoing operations for removal of a fibroadenoma or reduction mammoplasty. The day of the menstrual cycle was determined by hormone assay and direct questioning. Previously documented ultrastructural appea...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:A study has been made, using light and electron microscopy, of GMG cells in the labyrinthine placenta of the mouse. GMG cells were found lying within the lumina of the maternal blood spaces showed degenerative changes and GMG cells were usually found close to these degenerate trophoblast cells. Sometimes the GMG cells...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:The morphological transition from the simple epidermis that contacts the amniotic fluid of embryonic crocodilians to the adult epidermis required in a terrestrial environment has never been described. We used light and electron microscopy to study the development, differentiation and keratinisation of the epidermis of...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2001.19830265.x
更新日期：2001-03-01 00:00:00
abstract：:The unusual occurrence of skeletal muscle in the pineal gland of the bat Rhinopoma microphyllum is described. Individual muscle fibres were centrally placed and were incompletely encapsulated by a labyrinthine network of widened spaces filled with electron-dense material. The 2.4 micron long sarcomeres consisted of we...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The presence of nerve fibres in the medulla of the chick thymus has been investigated ultrastructurally. Unmyelinated nerve fibres were found not only in the perivascular space but also among the myoid cells. The nerve fibres were situated in close proximity to the myoid cells. The unmyelinated axons, containing predo...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Lateral cephalographs from 220 normal adolescent white orthodontic patients, and of individuals with craniofacial dysostosis and oculodento-osseous dysplasia were studied. Variations were observed in the morphology of the posterior margins of the superior articular processes, vertebral artery canals being present in 8...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:Pancreatic duct glands (PDGs) are tubule-alveolar glands associated with the pancreatic duct system and can be considered the anatomical counterpart of peribiliary glands (PBGs) found within the biliary tree. Recently, we demonstrated that endodermal precursor niches exist fetally and postnatally and are composed func...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12418
更新日期：2016-03-01 00:00:00
abstract：:A study has been made of the distribution of granulated metrial gland cells in the non-pregnant uterus, in the pre-implantation uterus and in the post-implantation interconceptual uterus of pregnant albino mice. Granulated cells were only rarely seen in the non-pregnant uterus. In the pre-implantation uterus, they wer...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:Amoeboid microglial cells (AMC) in the corpus callosum were selectively labelled following a single intraperitoneal (i.p.) injection of the fluorescent dye, rhodamine isothiocyanate (RhIc) into postnatal rats. The frequency of RhIc-labelled cells varied with age, with the largest number occurring in 7-d-old animals. T...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The rete testis in the domestic fowl (Gallus gallus domesticus), Japanese quail (Coturnix coturnix japonica), guinea-fowl (Numida meleagris galeata) and drake (Anas platyrhynchos) was studied histologically and with both the scanning and transmission electron microscopes. All the birds have rete epithelial cells varyi...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:The examination of endocranial data of archosauriforms has led to advances on the evolution of body size, nerve pathways, and sensory abilities. However, much of that research has focused on bird-line archosaurs, resulting in a skewed view of Archosauria. Phytosauria, a hypothesized sister taxon to or early-branching ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12668
更新日期：2017-11-01 00:00:00
abstract：:Little is known about the embryonic development and variation of the chondrocranium in Crocodylia and there are no works on any Caiman species. Due to the importance of cranial features in the systematics of this clade, investigating the development of the skull in embryonic stages is essential. In this study, we pres...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12968
更新日期：2019-06-01 00:00:00
abstract：:The present paper describes a novel structure, termed the peri-albumen layer, in the egg-envelopes of the quail Coturnix japonica. It reacts with Alcian blue and exists between the egg white and the shell membrane. Ultrastructurally, it is of fine granular structure and forms a fenestrate sheet, the width of which is ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2003.00197.x
更新日期：2003-07-01 00:00:00
abstract：:Although the development of the avian skeleton has attracted considerable attention, most of the studies have been concentrated on the embryonic period, while studies on the postnatal period are rare. We studied the postnatal development of the skeleton in two phylogenetically distant birds, an altricial passerine Acr...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13303
更新日期：2021-02-01 00:00:00
abstract：:The total number of muscle fibres, as well as the numbers of Type I, Type II and intermediate muscle fibre types, in transverse sections of the pectineus muscles of dogs of 2 and 12 months of age was determined by an indirect fibre counting method. The total nubmers of fibres in all muscles decreased very significantl...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Scales of snakes contain hard proteins (beta-keratins), now referred to as keratin-associated beta-proteins. In the present study we report the isolation, sequencing, and expression of a new group of these proteins from snake epidermis, designated cysteine-glycine-proline-rich proteins. One deduced protein from expres...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2009.01192.x
更新日期：2010-03-01 00:00:00
abstract：:A quantitative and morphological study was made of the corpus callosum in children to assess possible sex differences. The shape of the splenium of the corpus callosum was observed to vary between bulbous and cylindrical types with respect to the body of the callosum. The variation was not gender-specific and covariat...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:We previously identified a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present on the surface of human embryonal carcinoma stem cells, cells whose differentiation mimics early development. Antibodies reactive with various epitopes on this molecule define a cluster of differentiation markers ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2002.00034.x
更新日期：2002-03-01 00:00:00
abstract：:Blind mole-rats (Spalax ehrenbergi) are fossorial solitary rodents that present striking morphological, physiological and behavioural adaptations to the subterranean environment in which they live. Previous studies have shown that mole-rats are specialised in tooth-digging. The rapid eruption-rate of their incisors ha...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2001.19950591.x
更新日期：2001-11-01 00:00:00
abstract：:Integrating microdensitometry was used to study changes in the intracellular activity of 4 enzymes during macrophage development. Suspension cultures of blood monocytes from 19 healthy human subjects were examined at 0, 2, 4 and 6 d. Mononuclear phagocytes were harvested by glass adherence and standard methods were us...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The evolution of animal morphology can be understood as a series of changes in developmental programs. Among vertebrates, some developmental stages are conserved across species, representing particular developmental constraints. One of the most conserved stages is the vertebrate pharyngula, in which similar embryonic ...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/j.1469-7580.2005.00483.x
更新日期：2005-11-01 00:00:00
abstract：:The objective of the current study was to determine the tissue distribution of cells derived from the area opaca in heterospecific quail-chick blastodermal chimeras. Quail-chick chimeras were constructed by transferring dissociated cells from the area opaca of the stage X-XII (EG&K) quail embryo into the subgerminal c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2009.01168.x
更新日期：2010-01-01 00:00:00
abstract：:Cell abutments in the ovarian bursal epithelium of the golden hamster included tight junctions, desmosome-like junctions, gap junctions and zonulae adhaerentes. The plasma membranes of adjacent epithelial cells were often closely apposed forming a zonula adherens at the apex of the intercellular space, but these did n...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Trabecular bone microstructural parameters, including trabecular thickness, spacing, and number, have been reported to scale with animal size with negative allometry, whereas bone volume fraction is animal size-invariant in terrestrial mammals. As for the majority of scaling patterns described in animals, its underlyi...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12278
更新日期：2015-03-01 00:00:00
abstract：:The fine structure of the intercellular junctions of the hen parathyroid gland was studied using freeze-fracture replicas and thin sections. In the conventional thin sections, desmosomes, intermediate junctions (maculae adherentes) and gap junctions were observed, and in the lanthanum-fixed sections, tight junctions (...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:The mean fibre areas of samples from m. semitendinosus, m. diaphragma and m. pectoralis transversus of thoroughbred horses and greyhounds have been compared with those in similar samples from other members of their species. Athletes (greyhounds and thoroughbreds) have larger fibres in the semitendinosus and diaphragm ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：:A morphometric analysis was made of nuclei and cytoplasmic structures in electron micrographs of chondrocytes in the non-calcified layer of articular cartilage of the femoral condyles in adult mouse and dog and of the human incus. Mitochondrial volume density (% cytoplasm) was lower in dog than in mouse cells or cells...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The present study analyses the organization and selected neurochemical features of the claustrum and visual cortex of the sheep, based on the patterns of calcium-binding proteins expression. Connections of the claustrum with the visual cortex have been studied by tractography. Parvalbumin-immunoreactive (PV-ir) and Ca...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13302
更新日期：2021-01-01 00:00:00
