Abstract：
:We present the first complete, closed genome sequences of Streptococcus pyogenes strains NCTC 8198T and CCUG 4207T, the type strain of the type species of the genus Streptococcus and an important human pathogen that causes a wide range of infectious diseases. S. pyogenes NCTC 8198T and CCUG 4207T are derived from deposit of the same strain at two different culture collections. NCTC 8198T was sequenced, using a PacBio platform; the genome sequence was assembled de novo, using HGAP. CCUG 4207T was sequenced and a de novo hybrid assembly was generated, using SPAdes, combining Illumina and Oxford Nanopore sequence reads. Both strategies yielded closed genome sequences of 1,914,862 bp, identical in length and sequence identity. Combining short-read Illumina and long-read Oxford Nanopore sequence data circumvented the expected error rate of the nanopore sequencing technology, producing a genome sequence indistinguishable to the one determined with PacBio. Sequence analyses revealed five prophage regions, a CRISPR-Cas system, numerous virulence factors and no relevant antibiotic resistance genes. These two complete genome sequences of the type strain of S. pyogenes will effectively serve as valuable taxonomic and genomic references for infectious disease diagnostics, as well as references for future studies and applications within the genus Streptococcus.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Salvà-Serra F,Jaén-Luchoro D,Jakobsson HE,Gonzales-Siles L,Karlsson R,Busquets A,Gomila M,Bennasar-Figueras A,Russell JE,Fazal MA,Alexander S,Moore ERBdoi
10.1038/s41598-020-68249-ysubject
Has Abstractpub_date
2020-07-15 00:00:00pages
11656issue
1issn
2045-2322pii
10.1038/s41598-020-68249-yjournal_volume
10pub_type
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