Abstract：
:Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4-70 μmol Cs+ (L RBC)-1 s-1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kuchel PW,Karlsson M,Lerche MH,Shishmarev D,Ardenkjaer-Larsen JHdoi
10.1038/s41598-019-56250-zsubject
Has Abstractpub_date
2019-12-23 00:00:00pages
19726issue
1issn
2045-2322pii
10.1038/s41598-019-56250-zjournal_volume
9pub_type
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